scholarly journals Studi in silico potensi anthelmintik rambusa (Passiflora foetida) sebagai inhibitor produksi ATP pada Ascaris suum

2019 ◽  
Vol 3 (4) ◽  
pp. 79-80
Author(s):  
Dede Rival Novian ◽  
Aji Winarso
Keyword(s):  
In Silico ◽  
Ascaris Suum ◽  
Fumarate Reductase ◽  
Passiflora Foetida

Resistensi terhadap anthelmintika memacu upaya penemuan anthelmintika baru yang bekerja melalui penghambatan produksi energi ATP anaerobik pada sel parasit. Parasit melakukan metabolisme menggunakan enzim fumarat reduktase yang berfungsi untuk mengubah fumarat menjadi suksinat. Reaksi fumarat reduktase terjadi di mitokondria dengan bantuan rhodoquinone. Mitochondrial rhodoquinol-fumarate reductase  dianggap sebagai target reseptor yang baik untuk pengembangan anthelmintik baru. Penelitian ini bertujuan untuk memprediksi interaksi antara senyawa Passiflora foetida dengan reseptor mitochondrial rhodoquinol-fumarate reductase dari cacing Ascaris suum dengan metode in silico. Hasil penelitian menunjukkan bahwa senyawa bioaktif ermanin dan deidadlin dari Passiflora foetida memiliki potensi sebagai senyawa obat anthelmintik. Aktivitas senyawa ermanin dan deidaclin memiliki ernegi afinitias berturut turut-turut sebesar -8,2 kcal/mol dan -7,8 kcal/mol, bahkan lebih rendah dari senyawa Atpenin (-7,6kcal/mol)

scholarly journals Pharmacological Properties and Chemical Profiles of Passiflora foetida L. Extracts: Novel Insights for Pharmaceuticals and Nutraceuticals

Processes ◽  
2020 ◽  
Vol 8 (9) ◽  
pp. 1034
Author(s):  
Annalisa Chiavaroli ◽  
Simonetta Cristina Di Simone ◽  
Kouadio Ibrahime Sinan ◽  
Maria Chiara Ciferri ◽  
Giancarlo Angeles Flores ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
In Silico ◽  
Ex Vivo ◽  
Mouse Skin ◽  
Skin Inflammation ◽  
Water Extracts ◽  
Methanolic Extracts ◽  
In Silico Studies ◽  
Passiflora Foetida

In the present study, Passiflora foetida extracts characterized by different polarities were studied for their phytochemical profile, enzyme inhibitory, and antioxidant potentials. In silico, in vitro and ex vivo studies were also carried out on methanol and water extracts for predicting pharmacokinetics and pharmacodynamics. In this regard, neuronal HypoE22 cells, isolated mouse skin tissues, and pathogen dermatophytes strains were exposed to extracts. Emphasis was given to the preventing effects induced by the extracts on hydrogen peroxide-induced alterations of prostaglandin E2 (PGE2), l-dopa, and serotonin. Chemical analysis revealed the presence of similar compounds in infusion and methanolic extracts. The ex vivo studies also showed protective skin properties by P. foetida water and methanol extracts, as evidenced by the decrease of hydrogen peroxide-induced PGE2 level. Additionally, the blunting effects on hydrogen peroxide-induced l-dopa levels are consistent with the anti-tyrosinase effect exerted by both extracts. In silico studies demonstrated the affinity of extracts’ phytochemicals, namely apigenin, chrysoeriol, loliolide, luteolin, quercetin, and vitexin, towards cyclo-oxygenase-2 and tyrosinase. Finally, microbiological tests demonstrated the efficacy of P. foetida methanol and water extracts as anti-mycotic agents against Trichophyton and Arthroderma species, involved in skin inflammation. Hence, P. foetida L. extracts could represent potential sources of pharmaceuticals and nutraceuticals.

Decursin and Decursinol Angelate Selectively Inhibit NADH-Fumarate Reductase of Ascaris suum

Planta Medica ◽  
2007 ◽  
Vol 73 (14) ◽  
pp. 1478-1481 ◽  
Author(s):  
Kazuro Shiomi ◽  
Hiroko Hatano ◽  
Hiromi Morimoto ◽  
Hideaki Ui ◽  
Kimitoshi Sakamoto ◽  
...  
Keyword(s):  
Ascaris Suum ◽  
Fumarate Reductase ◽  
Decursinol Angelate

scholarly journals Cloning of a cDNA encoding the small subunit of cytochrome b558 (cybS) of mitochondrial fumarate reductase (complex II) from adult Ascaris suum

1996 ◽  
Vol 1276 (1) ◽  
pp. 1-5 ◽  
Author(s):  
Fumiko Saruta ◽  
Hiroko Hirawake ◽  
Shinzaburo Takamiya ◽  
Yu-Chang Ma ◽  
Takashi Aoki ◽  
...  
Keyword(s):  
Ascaris Suum ◽  
Small Subunit ◽  
Fumarate Reductase ◽  
Complex Ii ◽  
Cytochrome B558

The fumarate reductase system as a site of anthelmintic attack in Ascaris suum

1984 ◽  
Vol 4 (10) ◽  
pp. 879-883 ◽  
Author(s):  
Margaret M. Barrowman ◽  
Susan E. Marriner ◽  
James A. Bogan
Keyword(s):  
Enzyme Activity ◽  
Ascaris Suum ◽  
Fumarate Reductase ◽  
A Site

Various benzimidazole compounds have been shown to be highly eIIective as inhibitors (up to 50% reduction of activity) in vitro of the helminth-specilic enzyme, fumarate reductase, of Ascaris suum. Anthelmintically active and inactive benzimidazoles were similarly effective as inhibitors of enzyme activity. Albendazole-induced inhibition of Iumarate reductase was not observed when the enzyme was preincubated with NADH.

Crystal structure of mitochondrial quinol-fumarate reductase from the parasitic nematode Ascaris suum

2012 ◽  
Vol 151 (6) ◽  
pp. 589-592 ◽  
Author(s):  
H. Shimizu ◽  
A. Osanai ◽  
K. Sakamoto ◽  
D. K. Inaoka ◽  
T. Shiba ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Parasitic Nematode ◽  
Ascaris Suum ◽  
Fumarate Reductase
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