scholarly journals Effects of Exercise Preconditioning on Neurotrophin-4 and Tropomyosin Receptor Kinase B Expression in the Hippocampal CA1 Region Following Transient Global Cerebral Ischemia/Reperfusion in Wistar Rats

2019 ◽  
Vol 13 (6) ◽  
pp. 23-28
Author(s):  
Mohtaram Yazdanian ◽  
Mahtab Moazami ◽  
Mohammad Shabani ◽  
Sadegh Cheragh Birjandi ◽  
◽  
...  
Keyword(s):  
Wistar Rats ◽  
Ischemia Reperfusion ◽  
Global Cerebral Ischemia ◽  
Receptor Kinase ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Transient Global Cerebral Ischemia ◽  
Cerebral Ischemia Reperfusion ◽  
Hippocampal Ca1 ◽  
Exercise Preconditioning

scholarly journals Photobiomodulation Promotes Hippocampal CA1 NSC Differentiation Toward Neurons and Facilitates Cognitive Function Recovery Involving NLRP3 Inflammasome Mitigation Following Global Cerebral Ischemia

2021 ◽  
Vol 15 ◽  
Author(s):  
Sihan Guo ◽  
Ruimin Wang ◽  
Jiewei Hu ◽  
Liping Sun ◽  
Xinru Zhao ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Cerebral Ischemia ◽  
Ischemia Reperfusion ◽  
Neurological Recovery ◽  
Male Rats ◽  
Global Cerebral Ischemia ◽  
Inflammasome Activation ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Hippocampal Ca1

Our recent study revealed that photobiomodulation (PBM) inhibits delayed neuronal death by preserving mitochondrial dynamics and function following global cerebral ischemia (GCI). In the current study, we clarified whether PBM exerts effective roles in endogenous neurogenesis and long-lasting neurological recovery after GCI. Adult male rats were treated with 808 nm PBM at 20 mW/cm2 irradiance for 2 min on cerebral cortex surface (irradiance ∼7.0 mW/cm2, fluence ∼0.8 J/cm2 on the hippocampus) beginning 3 days after GCI for five consecutive days. Cognitive function was evaluated using the Morris water maze. Neural stem cell (NSC) proliferation, immature neurons, and mature neurons were examined using bromodeoxyuridine (BrdU)-, doublecortin (DCX)-, and NeuN-staining, respectively. Protein expression, such as NLRP3, cleaved IL1β, GFAP, and Iba1 was detected using immunofluorescence staining, and ultrastructure of astrocyte and microglia was observed by transmission electron microscopy. The results revealed that PBM exerted a markedly neuroprotective role and improved spatial learning and memory ability at 58 days of ischemia/reperfusion (I/R) but not at 7 days of reperfusion. Mechanistic studies revealed that PBM suppressed reactive astrocytes and maintained astrocyte regeneration at 7 days of reperfusion, as well as elevated neurogenesis at 58 days of reperfusion, as evidenced by a significant decrease in the fluorescence intensity of GFAP (astrocyte marker) but unchanged the number of BrdU-GFAP colabeled cells at the early timepoint, and a robust elevation in the number of DCX-NeuN colabeled cells at the later timepoint in the PBM-treated group compared to the GCI group. Notably, PBM treatment protected the ultrastructure of astrocyte and microglia cells at 58 days but not 7 days of reperfusion in the hippocampal CA1 region. Furthermore, PBM treatment significantly attenuated the GCI-induced immunofluorescence intensity of NLRP3 (an inflammasome component), cleaved IL1β (reflecting inflammasome activation) and Iba1, as well as the colocalization of NLRP3/GFAP or cleaved IL-1β/GFAP, especially in animals subjected to I/R at 58 days. Taken together, PBM treatment performed postischemia exerted a long-lasting protective effect on astrocytes and promoted endogenous neurogenesis in the hippocampal CA1 region, which might contribute to neurological recovery after GCI.

scholarly journals Rat B2Sequences Are Induced in the Hippocampal CA1 Region After Transient Global Cerebral Ischemia

1999 ◽  
Vol 274 (40) ◽  
pp. 28674-28681 ◽  
Author(s):  
Xiaodong Liu ◽  
James A. Clemens ◽  
Tinggui Yin ◽  
Diane T. Stephenson ◽  
Edward M. Johnstone ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Global Cerebral Ischemia ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Transient Global Cerebral Ischemia ◽  
Hippocampal Ca1

scholarly journals Effects of rhodioloside on the neurological functions of rats with total cerebral ischemia/reperfusion and cone neuron injury in the hippocampal CA1 region

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10056
Author(s):  
Yue Zhang ◽  
Xinqing Guo ◽  
Guohua Wang ◽  
Jidan Liu ◽  
Peiyu Liang ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Hippocampal Neurons ◽  
P53 Protein ◽  
Ischemia Reperfusion ◽  
Sham Operation ◽  
Protective Effects ◽  
Ca1 Region ◽  
Hippocampal Ca1 Region ◽  
Cerebral Ischemia Reperfusion ◽  
Hippocampal Ca1

Rhodioloside, the main effective constituent of Rhodiola rosea, demonstrates antiaging and antioxidative stress functions and inhibits calcium overloading in cells. These functions imply that rhodioloside may exert protective effects on hippocampal neurons after total cerebral ischemia/reperfusion injury. In this study, male Wistar rat models of total cerebral ischemia were constructed and randomly divided into four groups: sham-operation, ischemia/reperfusion, low-dosage, and high-dosage groups. The result showed that rhodioloside treatment reduced the apoptosis rates of hippocampal neurons and the histological grades of cone cells in the hippocampal CA1 region, but neuronal density was significantly increased. Besides, the protein expressions of Bcl-2/Bax and p53 were measured and found Bcl-2/Bax was increased and p53 protein level was reduced. Therefore, rhodioloside might have protective effects on rats with ischemia/reperfusion brain injury.

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