Protective effect of coded L-amino acids in cytostatic presence on the development of organotypic culture of tissues of different genesis

2021 ◽  
Vol 19 (2) ◽  
pp. 49-53
Author(s):  
Natalia Iosifovna Chalisova ◽  
Galina Anatolevna Ryzhak ◽  
Oleg Mikchailovich Ivko ◽  
Ecaterina Sergeevna Zalomaeva ◽  
Polina Nicolaevna Ivanova
Keyword(s):  
Amino Acids ◽  
Protective Effect ◽  
Organotypic Culture

PROTECTIVE EFFECT OF POLYPEPTIDE AND AMINO ACIDS ON THE DEVELOPMENT OF THE NERVOUS TISSUE CULTURE IN THE PRESENCE OF CYCLOPHOSPHAMIDE

2017 ◽  
pp. 22-26
Author(s):  
V. B. Dolgo-Saburov ◽  
N. I. Chalisova ◽  
L. V. Lyanginen ◽  
E. S. Zalomaeva
Keyword(s):  
Amino Acids ◽  
Cell Proliferation ◽  
Tissue Culture ◽  
Protective Effect ◽  
Organotypic Culture ◽  
Inhibitory Effect ◽  
Mustard Gas ◽  
Synthesis Inhibitor ◽  
Combined Administration ◽  
The Central Nervous System

In an organotypic culture, an investigation was conducted into combined effects of cyclophosphamide DNA as synthesis inhibitor used to model a resorptive action of mustard gas, and cortexin polypeptide or each of 20 encoded amino acids on the development of cell proliferation in cerebral cortex explants of the rat. The combined administration of cyclophosphamide together with cortexin or with each of the 20 encoded amino acids, except glycine, showed suppression of the cytostatic agent inhibitory effect. Thus, cortexin and amino acids have a protective effect on cell proliferation in the tissue culture of the central nervous system under the action of mustardlike substances.

Protective influence of coded amino acids on the development of liver tissue culture in the presence of cytostatic

2020 ◽  
pp. 48-53
Author(s):  
N. I. Chalisova ◽  
V. K. Kozlov ◽  
A. B. Mulik ◽  
E. P. Zatsepin ◽  
T. A. Kostrova
Keyword(s):  
Amino Acids ◽  
Tissue Culture ◽  
Side Effects ◽  
Liver Tissue ◽  
Organotypic Culture ◽  
Growth Zone ◽  
Inhibitory Effect ◽  
Cytostatic Drugs ◽  
Urgent Problem ◽  
Combined Exposure

An urgent problem is the search for substances that can provide a protective effect in cases of DNA synthesis and repair disorders that arise as a result of side effects of cytostatic drugs used in the treatment of cancer. The aim of this work was to study the effect of 20 encoded amino acids in the presence of Cyclophosphane on the development of organotypic culture of rat liver tissue. The results obtained indicate that Cyclophosphane; which simulates the action of such cytostatic substances; inhibits cell proliferation in the liver tissue. It was also found that the encoded amino acids: asparagine; arginine; and glutamic acid; eliminate the inhibitory effect of Cyclophosphane in liver tissue culture. The growth zone of explants after combined exposure to Cyclophosphane (whose isolated action suppressed the growth zone) and these amino acids increased significantly and reached control values. Thus; the experimental data create the basis for the development of methods for the therapeutic use of the three studied amino acids for the removal of side effects in the treatment with cytostatic drugs.

The protective effect of amino acids on the copper(II)-catalyzed autoxidation of ascorbic acid

2020 ◽  
Vol 16 (6) ◽  
Author(s):  
F. Imer ◽  
E. Aldemir ◽  
H. Kiliç ◽  
I. Sonmezoǧlu ◽  
R. Apak
Keyword(s):  
Amino Acids ◽  
Ascorbic Acid ◽  
Protective Effect

scholarly journals Immunogenicity and Safety of a Chemically Synthesized Divalent Group A Streptococcal Vaccine

2018 ◽  
Vol 2018 ◽  
pp. 1-7 ◽  
Author(s):  
Yongxiang Wu ◽  
Suhua Li ◽  
Yanting Luo ◽  
Yunyue Zhao ◽  
Jiarui Wang ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Amino Acids ◽  
Survival Rate ◽  
Protective Effect ◽  
Health Problem ◽  
Group A Streptococcus ◽  
Peptide Vaccine ◽  
Effective Vaccine ◽  
Histopathological Analysis ◽  
Group A

Background. Group A streptococcus (GAS) infections and poststreptococcal sequelae remain a health problem worldwide, which necessitates searching for an effective vaccine, while no licensed GAS vaccine is available. We have developed a divalent peptide vaccine composed of 84 amino acids to cover the main GAS serotypes (M1 and M12 streptococci) in China, and herein, we aimed to evaluate immunogenicity and safety of this vaccine.Methods. Mice were immunized with the vaccine. ELISA, indirect bactericidal test, and immunofluorescent assay were used to study immunogenicity. GAS challenge assay was used to test the protective effect. Safety was tested by histopathological analysis.Results. Immunized group mice (n=16) developed higher titer antibody after immunization than nonimmunized group mice (n=16) did. This antibody can deposit on the surface of GAS and promote killing of GAS, resulting in 93.1% decrease of M1 GAS and 89.5% of M12 GAS. When challenged with M1 and M12 streptococci, immunized group mice had a higher survival rate (87.5% and 75%) than nonimmunized group mice (37.5% and 25%). No autoimmune reactions were detected on organs of mice.Conclusion. The results suggest that this vaccine shows fair immunogenicity and safety, which will lead our research on GAS vaccine into clinical trial.

scholarly journals EFFECT OF AMINO ACID ON THE DEVELOPMENT SKIN TISSUE ORGANOTYPIC CULTURE

2017 ◽  
Vol 19 (3) ◽  
pp. 71-76
Author(s):  
Natal'ya I Chalisova ◽  
Aleksander E Korovin
Keyword(s):  
Adverse Effect ◽  
Amino Acids ◽  
Tissue Culture ◽  
Cellular Proliferation ◽  
Organotypic Culture ◽  
Growth Zone ◽  
Rat Skin ◽  
Inhibiting Effect ◽  
Combined Action ◽  
Organotypic Tissue

The effect of the 20 coded amino acids was investigated on the development of the processes of the proliferation in the organotypic tissue culture of rat skin. The amino acids lysine, arginine and glutamine acid at 0,05 ng/ml concentration stimulated the cellular proliferation in the growth zone of explants by 29-30%. The mustard-like agent cyclophosphane at 1 mg/ml concentration inhibited the cellular proliferation. The delay of this inhibiting effect of cyclophosphane was observed by the combined action of stimulating amino acids with the cyclophosphane. Thus, the amino acids with the charge radicals can be protectors of the cellular proliferation by the toxic effect of the cyclophosphane on the skin. This effect can be used for the treatment of the mustard injury of skin and for the delay of the adverse effect of cytostatic in oncology (1 figure, 1 table, bibliography: 28 refs).

scholarly journals Protective Effect of N-Acyl Amino Acids (NAAs) on Cephaloridine (CER) Nephrotoxicity in Rabbits

1993 ◽  
Vol 63 (4) ◽  
pp. 487-494 ◽  
Author(s):  
Yasukuni Hirouchi ◽  
Hideo Naganuma ◽  
Yukinori Kawahara ◽  
Ryuzo Okada ◽  
Akira Kamiya ◽  
...  
Keyword(s):  
Amino Acids ◽  
Protective Effect
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