scholarly journals DIABETIC FOOT INFECTION

2015 ◽  
Vol 22 (11) ◽  
pp. 1415-1422
Author(s):  
Abdul Ghani Rahimoon ◽  
Muhammad Tanveer Alam ◽  
Muhammad Saeed Talpur
Keyword(s):  
Diabetic Foot ◽  
Tertiary Care ◽  
Life Forms ◽  
Resistance Rate ◽  
Care Hospital ◽  
Gram Negative ◽  
Diabetic Foot Infection ◽  
Staph Aureus ◽  
Culture Growth ◽  
Grade 3

Objectives: To determine the frequency of common bacterial isolates culturedfrom diabetic foot infection in patients with type 2 diabetes mellitus (DM) falling in Wagner’sgrade-2 and grade-3 classification of diabetic foot (DF) infection. Study Design: Descriptivestudy. Period: A six months. Setting: Dow University of health sciences and civil hospital Karachi.Methods: Completed to examine the bacterial identification in cases admitted with the infectiondiabetic foot along with gave Wagner’s evaluation 2 and 3 at tertiary care hospital Karachi.Bacteriological finding and anti-biotic affectability profiles were completed and analyzed withutilizing standard strategies. Results: Out of 115 cases, 82 (71%) were male and 23 (29%) werefemale. The mean age of patients was 51.7 ±9.45 years, mean duration of diabetes was 10.6± 4.73 years, similarly mean length of time of diabetes foot wound was 46.15±23.75 days. 45(39%) patients had Wegner’s evaluation 2 and 70 (61%) patients had Wagner’s evaluation 3. 99cases indicated with culture growth, out of which 65 (65.65%) with gram negative microbes and25 (25.25%) gram-positive microbes. The most successive bacteria’s were Proteus (35.35%),Staph. Aureus (25.25%), Klebsiella (16.16%) and Pseudomonas (15.15%). Both gram positiveand gram negative showed frequent resistance to Cloxacillin, Amoxacillin, Levofloxacin, andLinezolid, gram negative life forms likewise indicated high resistance rate to Clindamycin,Vancomycin, and Cefotaxime. Tienam (Imipenem), Sulzone (salbactam in addition tocefoperazone) and Amikacin were the best effective against gram -ve and gram +vemicrobes.Staph. Aureus and Staph. Epidermidis were profoundly susceptible to Ciprofloxacin, Ceftriaxone,Clindamycin, and Vancomycin. Conclusion: Gram negative microbes were more common thangram positive living beings. Proteus, Staph Aureus, Klebsiella and Pseudomonas aeruginosawere the most widely recognized microorganisms of DF infection. Tienam (Imipenem), Sulzone(salbactam in addition to cefoperazone), and Amikacin were best effective agents.

scholarly journals COMMONLY ISOLATED ORGANISM IN DIABETIC FOOT AND ITS ANTIBIOTIC SENSITIVITY, AN EXPERIENCE AT TERTIARY CARE HOSPITAL

2020 ◽  
Vol 11 (1) ◽  
pp. 23-30
Author(s):  
Muhammad Iqbal Khan ◽  
Riffat Arbab. ◽  
Abdullah Khan ◽  
Maria Mehmood ◽  
Aisha Arshad ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Gram Negative Bacteria ◽  
Care Hospital ◽  
Gram Negative ◽  
Culture Sensitivity ◽  
Ethical Criteria ◽  
Aseptic Conditions

Abstract BACKGROUND & OBJECTIVE: To determine the commonly isolated organism in ulcers of diabetic foot and its sensitivity to antibiotics. METHODOLOGY: A total of 167 patients of diabetic foot were included in this descriptive Crosssectional study. All the patients were informed and consent was obtained according to ethical criteria approved by the ethical committee. The use of antibiotics in last 72 hours was strictly observed. The samples were obtained under aseptic conditions by applying the swap slightly to the exudate or base of the ulcer and were then carefully transferred in to the container and were then sent to the laboratory on the same date. The culture sensitivity was performed. Data was analyzed using SPSS. 20. RESULTS: A total of 140 samples were positive for 8 types of bacteria out of 167. 94 samples were monomicrobial were as 46 were polymicrobial. Over all Staphylococcus aureus 63(40.3%) was the most commonly isolated bacteria followed by Pseudomonas aeruginosa 40 (25.6%). S. aureus was most sensitive to imipenem/ meropenem (79.3%) followed by vancomycin (71%), linezolid (69.8%) and moxifloxacin (69.8%). P. aeruginosa was sensitive to impenem/ meropeneum (90%) followed by Ticarcilline/ clavulante (92.5%), amikacin (87.5%) and pipracilline / tazobactom (80%). Most of the gram positive and negative bacteria were resistant to commonly available antibiotic like ampiciline/ cloxacillin, amoxicilline/ clavulante and cephradine.  CONCLUSION: Most of the commonly used antibiotics had developed resistance. was S. aureus most common bacteria from the isolates and was sensitive to impenem/meropenem, vancomycin and linezolid. Gram-negative bacteria showed sensitivity to impenem/meropeneum, pipracilline/ tazobactom, Ticarcilline/ clavulante and amikacin.

scholarly journals A Comparison of Tissue versus Swab Culturing of Infected Diabetic Foot Wounds

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Ying Huang ◽  
Ying Cao ◽  
Mengchen Zou ◽  
Xiangrong Luo ◽  
Ya Jiang ◽  
...  
Keyword(s):  
Diabetic Foot ◽  
Prospective Trial ◽  
Punch Biopsy ◽  
Gram Negative Bacteria ◽  
Deep Tissue ◽  
Gram Negative ◽  
Diabetic Foot Infection ◽  
E Coli ◽  
Grade 3 ◽  
Tissue Specimens

Objective. To compare the efficacy of swabbing versus tissue biopsy for microbiological diagnosis of diabetic foot infection.Methods. This was a prospective trial. Fifty-six patients with diabetic foot infection were divided into the following 3 groups according to the PEDIS grading system: grade 2 (n=10), grade 3 (n=29), and grade 4 (n=17). Two specimens were collected from each wound for microbial culturing after debridement, including a superficial swab and a deep tissue punch biopsy specimen.Results. Swab culturing identified all of the microorganisms isolated from the corresponding deep tissue specimens in 9/10 of grade 2 wounds (90.0%), and this proportion decreased to 12/29 (41.4%) and 7/17 (41.2%) for grades 3 and 4 wounds, respectively (p=0.02). Moreover, the sensitivity for identifying Gram-negative bacteria, such asE. coliandCitrobacter, by swabbing was low (33.3%). In addition, some Gram-negative bacteria, such asSerratiaandRalstonia pickettii, were isolated from deep tissues but not from swabs.Conclusions. Swab culturing may be reliable for identification of pathogens in diabetic foot wounds classified as grade 2. However, it is advisable to culture deep tissue specimens for wounds of grade ≥3 because swab culturing is associated with a high risk of missing pathogens, especially Gram-negative bacteria.

scholarly journals Microbiological Profile and Antibiotic Sensitivity Pattern of Bacteria in Diabetic Foot Ulcers in a Tertiary Care Hospital

2021 ◽  
Vol 08 (01) ◽  
pp. 1-8
Author(s):  
Gajender Singh Ranga ◽  
Keyword(s):  
Diabetic Foot ◽  
Tertiary Care ◽  
Klebsiella Pneumonia ◽  
Care Hospital ◽  
Gram Positive ◽  
Cross Sectional ◽  
Gram Negative ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Sensitivity Pattern

Introduction: Diabetic foot is any foot pathology due to diabetes or sequelae of diabetes mellitus. This study was conducted to identify the common microorganisms isolated from diabetic foot and to analyse the antibiotic susceptibility pattern of bacteria isolated from diabetic foot. Materials and Method: The study was a hospital based cross-sectional study where 146 foot ulcer samples (who had type 2 diabetes) were analysed. Swabs were collected from the edge and margins of ulcers, and organisms were identified by gram staining culture and biochemical reactions. Result:Out of 146 patients, 69 specimens showed growth of organisms. Total 84 aerobic organisms were isolated and out of them, 64 cases showed bacterial growth, in which 84 bacteria were isolated, which represented an average of 1.28 organisms per case. Among these organisms, 62 gram-negative and 22 gram-positive organisms were isolated. E. coli was the most common gram-negative isolate (23.81%), followed by Pseudomonas aeruginosa (21.4%), and Klebsiella pneumonia(8.33%), while among gram-positive bacteria, S. aureus was the most common isolate (22.6%). Conclusion: Incidence of growth was 47.2% in which E. coli (23.8%) was the most common isolate. Gram-negative bacteria were more common than gram-positive bacteria. Diabetic foot infections are polymicrobial in nature.

scholarly journals Antibiotic susceptibility profiling in diabetic foot ulcer patients and evaluating treatment outcomes at a tertiary care hospital

2020 ◽  
Vol 7 (5) ◽  
pp. 1607
Author(s):  
T. J. Prasanna Kumar ◽  
Hari Babu Ramineni ◽  
Reshma Shaik ◽  
Suma Navya Yellavula ◽  
Virajitha Chandra
Keyword(s):  
Diabetic Foot ◽  
Tertiary Care ◽  
Foot Ulcer ◽  
Causative Organism ◽  
Care Hospital ◽  
Gram Positive ◽  
Klebsiella Species ◽  
Gram Negative ◽  
E Coli ◽  
Microbial Invasion

Background: Diabetic foot infections are the predominant complications of diabetes mellitus with uncontrolled hyperglycemia levels. Multiple microbial invasion is the primary cause and the causative organism are aerobic gram positive cocci, gram negative bacilli such as Pseudomonas species, Escherichia coli, Klebsiella species, Proteus species and anaerobes.Methods: This is a prospective, observational study conducted in the Department of General Surgery. 50 patients constituted the sample in our study. Patients pus and wound swab samples were collected using sterile and moist swab sticks under aseptic conditions and processed.Results: More gram negative bacteria (66%) were isolated than gram positive bacteria (34%). E. coli (38%) is the major isolated microorganism. Among the isolated organisms many of them are susceptible to amikacin (60%), imipenem (52%) and ciprofloxacin (46%) respectively.Conclusions: E. coli is the most common cause of infection. The antibiogram study showed an incidence of multiple resistant microorganisms to commonly used antibiotics.

scholarly journals Microbiological profile and Drug Resistant Organism’s pattern in Diabetic Foot Ulcer Patients at Tertiary Care Hospital Puducherry

2020 ◽  
Vol 11 (4) ◽  
pp. 6692-6697
Author(s):  
Jayarani Manikandan ◽  
Jaikumar S ◽  
Sandhya Rani T
Keyword(s):  
Diabetic Foot ◽  
Tertiary Care ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Type Ii Diabetes Mellitus ◽  
Multidrug Resistant ◽  
Gram Negative Bacteria ◽  
Care Hospital ◽  
Drug Resistant ◽  
Gram Negative

Type II diabetes mellitus is a significant health problem that developed globally. This study was carried out on patients with diabetic foot ulcer (DFU) to assess the bacterial and fungal flora, susceptibility, and drug-resistant isolates and devises an empiric antimicrobial therapy. Clinical data and patient samples were collected from 300 diabetic foot ulcer patients between September 2014, and September 2016 and samples were processed as per CLSI guidelines. Most of the pathogenic isolate recovered according to the Wagner classification system in DFU. The most commonly found isolates in our Study was Pseudomonas aeruginosa (22%), Staphylococcus aureus (15%) Escherichia coli (11%) followed by others. Antimicrobial resistance appears in aerobic, anaerobic as well as candida isolates in our study. Our results show most gram-negative bacteria were sensitive to colistin and tigecycline, and 44% of Gram-negative bacteria were ESBL producers, and among 20% of the gram-negative isolates were Multidrug resistant (MDR) organisms. Proper diagnosis of the causative agents, surveillance monitoring on the susceptibility of the isolates and determining the drugs for the empirical treatment of diabetic foot ulcers will prevent prolonged hospital stay and amputation.

scholarly journals A Bacteriological Study of Diabetic Foot Infection in an Urban Tertiary Care Hospital in Dhaka City

1970 ◽  
Vol 3 (2) ◽  
pp. 50-54
Author(s):  
Samir Paul ◽  
Lovely Barai ◽  
Ashraf Jahan ◽  
J Ashraful Haq
Keyword(s):  
Staphylococcus Aureus ◽  
Diabetic Foot ◽  
Diffusion Method ◽  
Foot Ulcers ◽  
Care Hospital ◽  
Esbl Production ◽  
Bacteriological Study ◽  
Gram Negative ◽  
Diabetic Foot Infection ◽  
Gram Negative Organisms

Identification of organisms and effective antibiotic therapy is an important component of treatment of diabetic foot infections. This study was undertaken to determine the organisms associated with diabetic foot infection (DFI) and their antibiotic sensitivity pattern. A total of 75 patients having type 2 diabetes mellitus with Wagner's grade 1-5 foot ulcers attending BIRDEM hospital were included in the study. Specimens were processed for aerobic culture. The bacteriological isolation and antimicrobial sensitivity tests of the isolates were done by standard microbiological methods. Gram negative bacilli were tested for extended spectrum â lactamase (ESBL) production by double disc diffusion method. Culture was positive in 92% of the cases which yielded 135 pathogens. Of the positive culture, 75.3% had multiple organisms. Polymicrobial infection was more in higher grade of foot ulcers. Gram negative organisms were most frequently isolated (80%) bacteria. Pseudomonas (48%) and Proteus sp.(33%) was the most common Gram negative organisms isolated. Staphylococcus aureus was the most commonly isolated gram positive organism (21.3%). ESBL production was noted in 31.5% Gram negative bacilli and methicillin resistance was noted in 43.8% of Staphylococcus aureus. Most of the Gram negative bacilli were resistant to various classes of antibiotics. Imepenem was the most effective agent against Gram negative organisms, while vancomycin was for staphylococcus. The present study has shown that infection with multidrug resistant Gram negative bacilli is the most common cause of DFI in BIRDEM hospital. Ibrahim Med. Coll. J. 2009; 3(2): 50-54   DOI: 10.3329/imcj.v3i2.4216

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