scholarly journals The Effect of Variations in Concentration Plasticizer Sorbitol on the Characteristics of Edible Film Made from Tilapia Fish Skin Gelatin (Oreochromis niloticus)

Author(s):  
Lina Anggraini ◽  
Eddy Suprayitno
Author(s):  
László G. Kömüves ◽  
Donna S. Turner ◽  
Kathy S. McKee ◽  
Buford L. Nichols ◽  
Julian P. Heath

In this study we used colloidal gold probes to detect the intracellular localization of colostral immunoglobulins in intestinal epithelial cells of newborn piglets.Tissues were obtained from non-suckled newborn and suckled piglets aged between 1 hour to 1 month. Samples were fixed in 2.5 % glutaraldehyde, osmicated and embedded into Spurr’s resin. Thin (80 nm) sections were etched with 5% sodium ethoxide for 5 min, washed and treated with 4 % sodium-m-periodate in distilled water for 30 min. The sections were then first incubated with blocking buffer (2 % BSA, 0.25 % fish skin gelatin, 0.5 % Tween 20 in 10 mM Trizma buffer, pH=7.4 containing 500 mM NaCl) for 30 min followed by the immunoreagents diluted in the same buffer, 1 hr each. For the detection of pig immunoglobulins a rabbit anti-pig IgG antiserum was used followed by goat anti-rabbit IgG-Au10 or protein A-Au15 probes.


2015 ◽  
Vol 48 ◽  
pp. 248-259 ◽  
Author(s):  
Phakawat Tongnuanchan ◽  
Soottawat Benjakul ◽  
Thummanoon Prodpran ◽  
Krisana Nilsuwan

2019 ◽  
Vol 19 ◽  
pp. 7-15 ◽  
Author(s):  
Maria do Livramento Linhares Rodrigues Menezes ◽  
Natália da Rocha Pires ◽  
Pablyana Leila Rodrigues da Cunha ◽  
Morsyleide de Freitas Rosa ◽  
Bartolomeu Warlene Silva de Souza ◽  
...  

2020 ◽  
Vol 101 ◽  
pp. 105537 ◽  
Author(s):  
Xiangjun Li ◽  
Xiang Liu ◽  
Keqiang Lai ◽  
Yuxia Fan ◽  
Yongle Liu ◽  
...  

2016 ◽  
Vol 56 ◽  
pp. 93-107 ◽  
Author(s):  
Phakawat Tongnuanchan ◽  
Soottawat Benjakul ◽  
Thummanoon Prodpran ◽  
Supachai Pisuchpen ◽  
Kazufumi Osako

2018 ◽  
Vol 19 (2) ◽  
pp. 618 ◽  
Author(s):  
Fei Liu ◽  
Furkan Türker Saricaoglu ◽  
Roberto Avena-Bustillos ◽  
David Bridges ◽  
Gary Takeoka ◽  
...  

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