Identification of Anthocyanins in Red Grape, Plum and Capulin by MALDI-ToF MS

A simple and fast method was developed to identify anthocyanins in red grapes, plums and capulins by MALDI-ToF MS. Three different matrices were used: α-CHCA (α-cyano-4-hydroxycinnamic acid), THA (2’,4’,6’-trihydroxyacetophenone) and DHBA (2,5-dihydroxybenzoic acid). Post-Source Decay (PSD) fragment detection allowed studying the structure of compounds detected. Deprotonated anthocyanin species were assigned in capulin ([Cyanidin-3-glucosidedeprotonated] K<sup>+</sup> and [Cyanidin-3-rutinoside-deprotonated]K<sup>+</sup>), due to these compounds also can be ionized in form of potassium adducts combined with a deprotonation.

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Characterization of a novel Sepia officinalis neuropeptide using MALDI-TOF MS and post-source decay analysis

Peptides ◽

10.1016/s0196-9781(01)00480-6 ◽

2001 ◽

Vol 22 (9) ◽

pp. 1391-1396 ◽

Cited By ~ 2

Author(s):

L.F. Marvin ◽

C. Zatylny ◽

J. Leprince ◽

H. Vaudry ◽

J. Henry

Keyword(s):

Sepia Officinalis ◽

Post Source Decay ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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A fast method for the determination of the PC/LPC ratio in intact serum by MALDI-TOF MS: An easy-to-follow lipid biomarker of inflammation

Chemistry and Physics of Lipids ◽

10.1016/j.chemphyslip.2014.07.001 ◽

2014 ◽

Vol 183 ◽

pp. 169-175 ◽

Cited By ~ 15

Author(s):

Roberto Angelini ◽

Gerrit Vortmeier ◽

Angela Corcelli ◽

Beate Fuchs

Keyword(s):

Fast Method ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Lipid Biomarker ◽

Tof Ms

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Combination of Microwave-Assisted Girard Derivatization with Ionic Liquid Matrix for Sensitive MALDI-TOF MS Analysis of Human Serum N-Glycans

Journal of Analytical Methods in Chemistry ◽

10.1155/2018/7832987 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Hoa Thi Le ◽

Kyu H. Park ◽

Woong Jung ◽

Hyung Soon Park ◽

Tae Woo Kim

Keyword(s):

Ionic Liquid ◽

Human Serum ◽

Solid Phase ◽

Liquid Matrix ◽

Dihydroxybenzoic Acid ◽

Established Method ◽

Maldi Tof Ms ◽

Microwave Assisted ◽

Maldi Tof ◽

Tof Ms

We developed a new method for MALDI-TOF MS detection of N-glycans derived from human serum. The synergistic combination of microwave-assisted Girard T derivatization, solid-phase extraction desalting, and an ionic liquid matrix (2, 5-dihydroxybenzoic acid/aniline) (GT-SPE-DHB/An) allowed of more sensitive N-glycans detection than a conventional ionic liquid matrix in MALDI-TOF MS. The superior sensitivity of our method was confirmed by the number of assigned N-glycans in 900–2,000 m/z range. Using our GT-SPE-DHB/An method, we were successfully able to assign 31 glycans. However, with the established method, i.e., DHB/An method, only 15 glycans were assigned. To the best of our knowledge, this GT-SPE-DHB/An method is the first to combine cationic derivatization of N-glycan and ionic liquid matrix for N-glycan analysis in MALDI-TOF MS.

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A boronic acid modified binary matrix consisting of boron nitride and α-cyano-4-hydroxycinnamic acid for determination of cis-diols by MALDI-TOF MS

Microchimica Acta ◽

10.1007/s00604-019-3711-3 ◽

2019 ◽

Vol 186 (8) ◽

Cited By ~ 1

Author(s):

Ya-Shun Chen ◽

Hua-Ming Xiao ◽

Tian-Qi Wang ◽

Lei Yu ◽

Yu-Qi Feng

Keyword(s):

Boron Nitride ◽

Boronic Acid ◽

Hydroxycinnamic Acid ◽

Binary Matrix ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Tof Ms

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Detection of Adducts with Matrix Clusters in the Positive and Negative Ion Mode MALDI-TOF Mass Spectra of Phospholipids

Zeitschrift für Naturforschung B ◽

10.1515/znb-2009-0314 ◽

2009 ◽

Vol 64 (3) ◽

pp. 331-334 ◽

Cited By ~ 9

Author(s):

Marijana Petković ◽

Jürgen Schiller ◽

Matthias Müller ◽

Rosmarie Süß ◽

Klaus Arnold ◽

...

Keyword(s):

Mass Spectra ◽

Negative Ion ◽

Dihydroxybenzoic Acid ◽

Maldi Tof Ms ◽

Maldi Tof ◽

Maldi Tof Mass Spectra ◽

Negative Ion Mode ◽

Charged Ions ◽

Positively Charged ◽

Tof Ms

It is usually accepted that neutral phospholipids (PLs) generate singly positively charged ions, whereas negative PLs are easily detectable in the negative ion mode when analysed by matrix-assisted laser desorption and ionisation time-offlight mass spectrometry (MALDI-TOF MS). In this study, we demonstrate that some caution is required in the interpretation of MALDI-TOF mass spectra of PLs, since also neutral PLs have appeared to be detectable in the negative ion mode as well. Neutral and negatively charged phospholipids can generate adducts with the most commonly used matrix - 2,5-dihydroxybenzoic acid - yielding singly negatively charged ions that are detectable in the spectra. This further contributes to the complexity of the spectra and potentially leads to severe misinterpretation, particularly when unknown mixtures of PLs are analysed by MALDI-TOF MS.

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