scholarly journals DETECTION OF GENETIC DETERMINANTS OF PATHOGENICITY OF STRAINS OF KLEBSIELLA SPP. ISOLATED FROM THE INTESTINAL BIOTOPE OF CHILDREN WITH FUNCTIONAL GASTROINTESTINAL DISORDERS

2018 ◽  
Vol 3 (5) ◽  
pp. 60-65
Author(s):  
E. V. Grigorova ◽  
L. V. Rychkova ◽  
E. I. Ivanova ◽  
U. M. Nemchenko ◽  
M. V. Savelkaeva
Keyword(s):  
Functional Gastrointestinal Disorders ◽  
Gastrointestinal Disorders ◽  
First Year ◽  
Genetic Determinants ◽  
Pathogenic Potential ◽  
Bacteriological Study ◽  
Industry Standard ◽  
Comparison Groups ◽  
First Year Of Life ◽  
Klebsiella Spp

Functional gastrointestinal disorders (FGID) are one of the most common problems in children of the first year of life. The aim of the study was to assess the pathogenic potential of Klebsiella spp. strains, isolated from the colon in children of the first year of life with FGID. Material for the study included 61 coprological samples. The biological material was divided into comparison groups, depending on the type of Klebsiella excreted at a concentration of 105–108 CFU/g: 1st – with vegetation in the colon K. pneumoniae (n = 30); 2nd – with vegetation K. oxytoca (n = 31). Bacteriological study composition of the intestinal contents was carried out according to the Industry standard “Protocol of management of patients. Intestinal dysbiosis” (2003). Identification was carried out according to generally accepted schemes using commercial test systems for biochemical identification of bacteria. Statistical data processing was performed using licensed applications “MS Office Excel 2003 for Windows 7”. The data on the quantitative and qualitative changes in the composition microbiota in the comparison groups were obtained. The results of detection genetic determinants of pathogenicity in the samples of Klebsiella of two species show that among the strains of Klebsiella spp., vegetating in the intestines of children as a component of an allochthonous microbiota, a sufficiently high and virulent potential can be concentrated. Detection of pathogenicity genes in bacteria of the genus Klebsiella will expand and deepen the problem of finding the structures of adaptation of strains of bacteria that cause FGID in children of the first year of life.

scholarly journals Neonatal Antibiotics and Prematurity Are Associated with an Increased Risk of Functional Gastrointestinal Disorders in the First Year of Life

2019 ◽  
Vol 212 ◽  
pp. 44-51 ◽  
Author(s):  
Silvia Salvatore ◽  
Maria Elisabetta Baldassarre ◽  
Antonio Di Mauro ◽  
Nicola Laforgia ◽  
Silvio Tafuri ◽  
...  
Keyword(s):  
Functional Gastrointestinal Disorders ◽  
Gastrointestinal Disorders ◽  
First Year ◽  
Increased Risk ◽  
First Year Of Life

Еvaluation of the sensitivity of bacteriophage preparations to Klebsiella pneumoniae strains isolated from the colon microbiota in children with functional gastrointestinal disorders

2021 ◽  
Vol 66 (4) ◽  
pp. 217-222
Author(s):  
E. V. Grigorova ◽  
L. V. Rychkova ◽  
N. L. Belkova ◽  
U. M. Nemchenko ◽  
M. V. Savelkaeva ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Functional Gastrointestinal Disorders ◽  
Lytic Activity ◽  
Gastrointestinal Disorders ◽  
Low Level ◽  
Industry Standard ◽  
Comparison Groups ◽  
Symptom Complex ◽  
Defecation Disorder

The level of sensitivity of Klebsiella pneumoniae strains isolated from children of the first half of life with functional gastrointestinal disorders (FGID) to commercial bacteriophage preparations was assessed. The material was the feces of 67 children of the first half of life with FGID who are breastfed. Culture of K. pneumoniae isolated from faeces of children, amounted to two comparison groups, depending on the age of the patients. The first group included 43 K. pneumoniae strains isolated from the colon of children aged from birth to three months, in the second group - 24 strains, from children aged three to six months. The composition of the colon microbiota was studied using standard methods, and the results were evaluated in accordance with Industry Standard 91500.11.0004-2003. Identification of K. pneumoniae was performed by bacteriological methods. Determination of the level of lytic activity of K. pneumoniae, Klebsiella polyvalent and sextaphage bacteriophages to K. pneumoniae strains was conducted by the drip method (spot-test) according to clinical recommendations. It is shown that the formation of FGID symptoms in children correlates with age - the frequency of regurgitation decreased in children up to six months (from 23.3% to 4.2%) against the background of the formation of a symptom complex associated with defecation disorder. Bacteriological analysis showed that in General, phages show a low level of lytic activity, sensitivity to them Klebsiella also correlated with age and was higher in children of three to six months. Thus, in the first comparison group, the absence of Klebsiella lysis in relation to the Klebsiella pneumoniae bacteriophage was registered three times more often (30.2%, p<0.05) and twice less often (30.2%, p<0.05), the low level of lysis of K. pneumoniae strains to the Klebsiella polyvalent phage compared to the second group. The sensitivity of K. pneumoniae strains to sextaphage was comparable in comparison groups and varied from 2 to 10%. Thus, among the studied K. pneumoniae observed a low level of sensitivity to specific drugs - bacteriophage Klebsiella pneumoniae, Klebsiella polyvalent and sextaphage. This fact reflects the insufficient activity of phages and predicts low effectiveness of empirical phage therapy without elimination from the intestinal biocenosis of K. pneumoniae in children with FGID who are breastfed.

scholarly journals Features of the Composition of the Colon Microbiota in Children of the First Year of Life with Functional Gastrointestinal Disorders

2021 ◽  
Vol 5 (6) ◽  
pp. 106-112
Author(s):  
E. V. Grigorova ◽  
L. V. Rychkova ◽  
L. F. Sholokhov ◽  
N. L. Belkova ◽  
U. M. Nemchenko ◽  
...  
Keyword(s):  
Functional Gastrointestinal Disorders ◽  
Gastrointestinal Disorders ◽  
First Year ◽  
First Year Of Life

Clostridium perfringens – antibiotic-associated and sporadic diarrhea in children: age, anamnestic and clinical and laboratory features

2021 ◽  
pp. 10-15
Author(s):  
A. S. Kvetnaya ◽  
L. I. Zhelezova
Keyword(s):  
Clostridium Perfringens ◽  
Enzyme Analysis ◽  
First Year ◽  
Saint Petersburg ◽  
Clinical Center ◽  
Colony Forming Units ◽  
Antibiotic Associated Diarrhea ◽  
First Year Of Life ◽  
Diarrhea Syndrome ◽  
Klebsiella Spp

The article presents the data of the research on the problem of two clinical forms of enteroproducing Clostridium perfringens (C. perfringens) – associated infection in the children who are not connected with food – antibiotic-associated and sporadic forms. Within the period of 2019–2021 a retrospective analysis of the results of complex clinical and laboratory study of children aged from 2 months to 18 years with diarrhea (acute intestinal infection, n = 406 children) was carried out within the framework of the algorithm developed by Pediatric Research and Clinical Center for Infectious Diseases (Russia, Saint Petersburg). The material for the study included stool tests, coprofiltrates and strains of the microorganisms determinating microbiocoenosis of the intestines including enterotoxigenic strains of C. perfringens. The main group included children with diarrhea (n = 38 of 406 children with diarrhea) who at admission presented C. perfringens enterotoxin in coprofiltrates found by immune-enzyme analysis and there were identified the cultures of enterotoxigenic strains of C. perfringens by tests of intestinal contents. The first group included children (n = 30 of 406 children; 7 %) with antibiotic-associated diarrhea (AAD). The second group included children (n = 8 of 406 children; 2 %) with sporadic diarrhea. An overwhelming majority of patients with ААD (n = 20; 92.3 %) of the first 8 (100 %) years of life presented intensity of diarrhea syndrome in direct dependence on colonization activity of enterotoxigenic C perfringens (colony-forming units/g feces) (r = 0.78). The disease proceeded mainly with the syndromes of gastroenteritis, enterocolitis and hemorrhagic colitis. There was noted a reliable high frequency of severe forms of both ААD, and sporadic diarrhea (р < 0.05) caused by enterotoxigenic strains of C perfringens in children of the first year of life. Deep decompensated disorders of large intestine microbiota associated with Klebsiella spp., Staphylococcus aureus and Candida albicans, were characterized by severity and prolonged character of the course (р < 0.05). The received results determine the tactics of differential diagnosis and treatment of AAD and sporadic diarrhea caused by enteroproducing strains of C. perfringens.

scholarly journals Lactobacillus reuteri DSM 17938 and vitamin D3 in common clinical problems of infants

2021 ◽  
pp. 68-72
Author(s):  
S.P. Kryvopustov ◽  
Keyword(s):  
Vitamin D ◽  
Vitamin D3 ◽  
Lactobacillus Reuteri ◽  
Respiratory Infections ◽  
Functional Gastrointestinal Disorders ◽  
Functional Constipation ◽  
Gastrointestinal Disorders ◽  
First Year ◽  
Infant Colic ◽  
Clinical Problems

Purpose — to demonstrate the clinical significance of Lactobacillus reuteri DSM 17938 and vitamin D3 in common clinical problems in infants. Materials and methods. Clinical cases in pediatrics of infancy in children with infant colic (G4), infant regurgitation (G1), functional constipation (G7) are presented. Some international recommendations regarding a preventive dose of vitamin D in the first year of a child' life are considered. Results. The behavior of an infant is often a concern for parents and is a reason for seeking medical help. In particular, it has been shown when acute crying of a child is associated with threatening conditions. The prevalence of functional gastrointestinal disorders and the importance of using the Rome IV criteria in practice are emphasized. Clinical examples of infant colic (G4), infant regurgitation (G1), functional constipation (G7) demonstrated the management of patients with the importance of counseling, showing the evidence base for the use of Lactobacillus reuteri DSM 17938 and vitamin D3. Works on the importance of vitamin D for the prevention of respiratory infections and its dosage are presented. Conclusions. For children in the first year of life with infant colic (G4), infant regurgitation (G1), functional constipation (G7), counseling is important, as well as the use of, in particular, Lactobacillus reuteri DSM 17938. Additional benefits are provided by the combined use of Lactobacillus reuteri DSM 17938 with vitamin D3, which is also discussed for the prevention of respiratory infections. Provided international recommendations for a daily preventive dose of 400 IU of vitamin D for infants. No conflict of interest was declared by the author. Key words: vitamin D, infants, respiratory infections, functional gastrointestinal disorders, Lactobacillus reuteri DSM 17938.

scholarly journals Analysis of health outcomes in vaccinated and unvaccinated children: Developmental delays, asthma, ear infections and gastrointestinal disorders

2020 ◽  
Vol 8 ◽  
pp. 205031212092534 ◽  
Author(s):  
Brian S Hooker ◽  
Neil Z Miller
Keyword(s):  
Developmental Delays ◽  
International Classification Of Diseases ◽  
International Classification ◽  
Health Conditions ◽  
Gastrointestinal Disorders ◽  
First Year ◽  
Classification Of Diseases ◽  
Ear Infections ◽  
First Year Of Life

Objective: The aim of this study was to compare the health of vaccinated versus unvaccinated pediatric populations. Methods: Using data from three medical practices in the United States with children born between November 2005 and June 2015, vaccinated children were compared to unvaccinated children during the first year of life for later incidence of developmental delays, asthma, ear infections and gastrointestinal disorders. All diagnoses utilized International Classification of Diseases–9 and International Classification of Diseases–10 codes through medical chart review. Subjects were a minimum of 3 years of age, stratified based on medical practice, year of birth and gender and compared using a logistic regression model. Results: Vaccination before 1 year of age was associated with increased odds of developmental delays (OR = 2.18, 95% CI 1.47–3.24), asthma (OR = 4.49, 95% CI 2.04–9.88) and ear infections (OR = 2.13, 95% CI 1.63–2.78). In a quartile analysis, subjects were grouped by number of vaccine doses received in the first year of life. Higher odds ratios were observed in Quartiles 3 and 4 (where more vaccine doses were received) for all four health conditions considered, as compared to Quartile 1. In a temporal analysis, developmental delays showed a linear increase as the age cut-offs increased from 6 to 12 to 18 to 24 months of age (ORs = 1.95, 2.18, 2.92 and 3.51, respectively). Slightly higher ORs were also observed for all four health conditions when time permitted for a diagnosis was extended from ⩾ 3 years of age to ⩾ 5 years of age. Conclusion: In this study, which only allowed for the calculation of unadjusted observational associations, higher ORs were observed within the vaccinated versus unvaccinated group for developmental delays, asthma and ear infections. Further study is necessary to understand the full spectrum of health effects associated with childhood vaccination.

scholarly journals Microbiocenosis of colon and ability to biofi lm formation of strains Klebsiella spp. in children with functional gastrointestinal disorders

2020 ◽  
Vol 174 (4) ◽  
pp. 59-64
Author(s):  
U. M. Nemchenko ◽  
E. A. Kungurtsevа ◽  
M. V. Savelkaeva ◽  
E. V. Grigorova ◽  
E. I. Ivanova ◽  
...  
Keyword(s):  
Functional Gastrointestinal Disorders ◽  
Gastrointestinal Disorders ◽  
Klebsiella Spp

scholarly journals FEATURES OF LARGE INTESTINE MICROBIOCENOSIS IN INFANTS WITH FUNCTIONAL DISORDERS OF GASTROINTESTINAL TRACT AGAINST HIGH POPULATION DENSITY OF BACTERIA OF THE GENUS KLEBSIELLA

2017 ◽  
Vol 1 (66) ◽  
pp. 83-88 ◽  
Author(s):  
Екатерина Григорова ◽  
Ekaterina Grigorova ◽  
Ульяна Немченко ◽  
Ulyana Nemchenko ◽  
Елена Иванова ◽  
...  
Keyword(s):  
Gastrointestinal Tract ◽  
Large Intestine ◽  
High Titer ◽  
Biochemical Properties ◽  
Selective Decontamination ◽  
First Year ◽  
Functional Disorders ◽  
Klebsiella Species ◽  
First Year Of Life ◽  
Klebsiella Spp

The aim of the research was to determine the features of large intestinal microbiocenosis during the vegetation of bacteria of the genus Klebsiella in children with functional disorders of the gastrointestinal tract in different age periods of the first year of life. The material for the study is based on 210 coprological samples isolated from infants of both sexes with functional disorders of the gastrointestinal tract and indication of Klebsiella spp. in the titer of 105-108 CFU/g. The children were grouped according to the age: 1st − children from 1 to 3 months old, 2nd − children from three to six months old, 3rd − children from 6 to 12 months old. All of them were divided into two groups for comparison, depending on the type of isolated Klebsiella species: K. pneumoniae (n=105) and K. oxytoca (n=105). The investigation of the intestinal microflora was performed in accordance with conventional methodology, and the assessment of the results was done according to Industry Standard. Identified bacteria were divided by their morphologic, tinctorial, cultural and biochemical properties. As a result of the study, dysbiotic disturbances of the 3rd degree were observed in children, complicated by the proliferation of bacteria of the genus Klebsiella, especially by its diagnostically important bacterial agent K. oxytoca. Most critical for the development of functional disorders of the gastrointestinal tract for the children of the first year of life is the age of 3-6 months. Long-term persistence of the Klebsiella spp. in high titer (105-108 CFU/g) in the large intestine of the children with functional disorders of the gastrointestinal tract dictates the need for selective decontamination of the Klebsiella from the gut microbiota.

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