The Potency of Bacillus siamensis LDR as Biocontrol Agent Against Fungal Phytopathogen

Anthracnose caused by Colletotrichum gloeosporioides is one of most serious fungal diseases on Chinese fir (Cunninghamia lanceolata). Eight fungal endophytes were isolated from a young heathy branch of Chinese fir and screened against the pathogen in vitro. One isolate, designated as SMEL1 and subsequently identified as Epicoccum dendrobii based on morphological and phylogenetic analyses, suppressed mycelial growth of C. gloeosporioides on dual culture plates. Additionally, the metabolites of E. dendrobii significantly decreased the biomass of C. gloeosporioides. E. dendrobii was able to enter the internal tissues of the host plant via stomatal cells. The metabolites of E. dendrobii significantly inhibited conidial germination and appressorium formation, which at least partly explained why the endophyte significantly inhibited lesion development caused by C. gloeosporioides on various host plants. We further confirmed that some components with antifungal activity could be extracted from E. dendrobii using ethyl acetate as an organic solvent. To our knowledge, this is the first report of E. dendrobii as a potential biocontrol agent against a fungal phytopathogen.

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Biology of the Introduced Biocontrol Agent Microctonus hyperodae (Hymenoptera: Braconidae) and Its Host Listronotus bonariensis (Coleoptera: Curculionidae) in Northern New Zealand

Environmental Entomology ◽

10.1603/en11246 ◽

2013 ◽

Keyword(s):

New Zealand ◽

Biocontrol Agent ◽

Listronotus Bonariensis ◽

Microctonus Hyperodae

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Incorporating olfactory cues into risk assessment of the root weevil,Mogulones crucifer,as a biocontrol agent ofCynoglossum officinale

10.1603/ice.2016.111534 ◽

2016 ◽

Author(s):

Basu D. Kafle

Keyword(s):

Risk Assessment ◽

Biocontrol Agent ◽

Olfactory Cues

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Preemptive assessment of the biosafety of a classical biocontrol agent: Responses ofTrissolcus japonicusto New Zealand’s native Pentatomidae prior to the arrival of BMSB

10.1603/ice.2016.110850 ◽

2016 ◽

Cited By ~ 2

Author(s):

John Gordon Charles

Keyword(s):

Biocontrol Agent ◽

Classical Biocontrol

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Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

Hellenic Plant Protection Journal ◽

10.2478/hppj-2020-0006 ◽

2020 ◽

Vol 13 (2) ◽

pp. 54-65 ◽

Cited By ~ 1

Author(s):

M.E.A. Bendaha ◽

H.A. Belaouni

Keyword(s):

Root Length ◽

Biocontrol Agent ◽

Growth Promotion ◽

Dry Weight ◽

Antagonistic Activity ◽

Rrna Gene ◽

Plant Growth Promoting ◽

Enterobacter Ludwigii ◽

Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

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What habitat does spiny snout mite occupy in Tasmania

New Zealand Plant Protection ◽

10.30843/nzpp.2015.68.5854 ◽

2015 ◽

Vol 68 ◽

pp. 446-446

Author(s):

D.J. Wilson ◽

P.J. Gerard

Keyword(s):

New Zealand ◽

Sand Dune ◽

Biocontrol Agent ◽

Predatory Mites ◽

Mite Species ◽

Predatory Mite ◽

Habitat Specificity ◽

Nontarget Organisms ◽

Flea Control ◽

Potential Biocontrol Agent

Spiny snout mite (Neomolgus capillatus) is a potential biocontrol agent for clover flea (Sminthurus viridis) a white clover pest on dairy farms in warmer and wetter parts of New Zealand In the 1990s this mite was introduced from Brittany France into Tasmania for clover flea control Results during the release programme were highly promising and subsequent anecdotal farmer reports indicate widespread decreases in damage As N capillatus is a predatory mite and already known to attack nontarget organisms habitat specificity will determine whether it could be introduced into New Zealand without risk to native insects To assess this pastures on nine of the original Tasmanian release farms and adjacent nontarget habitats ranging from bush wetlands eucalypt stands to sand dune country were sampled in April 2014 Litter samples were collected heat extracted and mite species identified Neomolgus capillatus was found at effective densities in pastures that had good clover cover Where present it displaced Bdellodes spp mites that are ineffective against clover flea No N capillatus were found in the nontarget habitats all of which lacked clover and contained other predatory mites including Bdellodes spp Therefore the preference by N capillatus for lush pastures makes it an excellent prospect for introduction as a biocontrol agent into clover flea prone regions of New Zealand

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Lecanicillium lecanii (Zimmermann) Zare & Gams, as an efficient biocontrol agent of tea thrips, Scirtothrips bispinosus Bagnall (Thysanoptera: Thripidae)

Egyptian Journal of Biological Pest Control ◽

10.1186/s41938-021-00380-y ◽

2021 ◽

Vol 31 (1) ◽

Author(s):

Mariappan Sankara Rama Subramaniam ◽

Azariah Babu ◽

Bhabesh Deka

Keyword(s):

Tamil Nadu ◽

South India ◽

Biocontrol Agent ◽

Uv Light ◽

Biological Control Agents ◽

Optimum Conditions ◽

Lecanicillium Lecanii ◽

Wettable Powder ◽

Label Claim ◽

Yeast Powder

Abstract Background Continuous and non-judicial application of synthetic insecticides to control the tea thrips, Scirtothrips bispinosus (Bagnall), one of the major tea pests in South India has led to certain undesirable issues in the ecosystem besides the presence of the pesticide residues in manufactured tea. Biological control agents are of immense importance in tea cultivation. The present study was designed to isolate Lecanicillium lecanii (Zimmermann) Zare & Gama from the field-collected cadavers of the insects/mites infected by fungi of tea growing areas of Anamallais (Tamil Nadu, South India), and to evaluate their field bio-efficacy against the tea thrips. Results Lecanicillium lecanii isolated from the tea ecosystem had been formulated into a wettable powder (WP) formulation and evaluated against tea thrips under both laboratory and field conditions. Among the several media evaluated, the PDAY (Potato Dextrose Agar + 1% Yeast powder) was found to be the best suitable medium for the growth and germination of spores. Optimum conditions for the growth of L. lecanii were found in PDAY medium at the pH 6-7, temperature 25-30°C and 90-95% RH. Exposure to UV light for more than 30 min significantly inhibited the growth of the fungus. Lecanicillium lecanii at (1 × 107 spore/ha) was found significantly effective against thrips. Fungal development index (FDI) of L. lecanii + jaggery significantly differed than other treatments. Lecanicillium lecanii at 1500g (1×107 conidia/ml) mixed in 400 l of water was effective against the tea thrips. Addition of equal amount of jaggery with L. lecanii wettable powder in the tank mixture could increase the efficacy of the mycopesticide against tea thrips. Conclusion The powder formulation of L. lecanii was found safer to natural enemies present in the tea ecosystem. After fulfilling the requirements for its registration and label claim on tea, this strain of L. lecanii could be commercialized for the benefit of the tea industry for the management of tea thrips in an eco-friendly manner.

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GroEL protein from the potential biocontrol agent Rhodopseudomonas palustris enhances resistance to rice blast disease

Pest Management Science ◽

10.1002/ps.6584 ◽

2021 ◽

Author(s):

Xiyang Wu ◽

Yue Chen ◽

Chenggang Li ◽

Xin Zhang ◽

Xinqiu Tan ◽

...

Keyword(s):

Rice Blast ◽

Biocontrol Agent ◽

Rhodopseudomonas Palustris ◽

Rice Blast Disease ◽

Blast Disease ◽

Groel Protein ◽

Potential Biocontrol Agent

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A Preliminary Assessment of Amblyseius andersoni (Chant) as a Potential Biocontrol Agent against Phytophagous Mites Occurring on Coniferous Plants

Insects ◽

10.3390/insects12080664 ◽

2021 ◽

Vol 12 (8) ◽

pp. 664

Author(s):

Ewa Puchalska ◽

Stanisław Kamil Zagrodzki ◽

Marcin Kozak ◽

Brian G. Rector ◽

Anna Mauer

Keyword(s):

Biocontrol Agent ◽

Mass Rearing ◽

Predator Population ◽

Preliminary Assessment ◽

Amblyseius Andersoni ◽

Food Items ◽

Oligonychus Ununguis ◽

Potential Biocontrol Agent ◽

Pine Pollen ◽

Survival And Reproduction

Development, survival and reproduction of Ambyseius andersoni (Chant), a predatory mite widely distributed in Europe, were assessed on different food items. These included two key pests of ornamental coniferous plants, i.e., Oligonychus ununguis (Jacobi) and Pentamerismus taxi (Haller) and pollen of Pinus sylvestris L. The rationale behind these experiments was to provide a preliminary assessment of the potential of A. andersoni as a biocontrol agent of the above phytophagous arthropods and evaluate pine pollen as an alternative food source for the predator. Under laboratory conditions (23 ± 0.5 °C, 70 ± 10% RH and 16L:8D) A. andersoni was able to feed, develop and reproduce on all tested diets. The shortest development time (egg to female) was obtained when the predator fed on P. taxi (mean = 5.12 d) and the longest was on pine pollen (mean = 6.55 d). The rm value was significantly higher on both tested prey (0.166 on P. taxi and 0.160 on O. ununguis) than on pollen (0.139). Thus, we do not recommend pine pollen for mass rearing of A. andersoni; however, we conclude that pollen may provide sufficient sustenance for the predator population under field conditions when prey are absent. The potential of A. andersoni as a biocontrol agent of O. ununguis and P. taxi is discussed.

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