scholarly journals The fungal endophyte Epicoccum dendrobii as a potential biocontrol agent against Colletotrichum gloeosporioides

2020 ◽  
Author(s):  
Jin-Yue Bian ◽  
Yu-Lan Fang ◽  
Qing Song ◽  
Mei-Ling Sun ◽  
Ji-Yun Yang ◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Biocontrol Agent ◽  
Phylogenetic Analyses ◽  
Fungal Endophytes ◽  
Fungal Endophyte ◽  
Chinese Fir ◽  
Dual Culture ◽  
Potential Biocontrol Agent ◽  
Fungal Phytopathogen

Anthracnose caused by Colletotrichum gloeosporioides is one of most serious fungal diseases on Chinese fir (Cunninghamia lanceolata). Eight fungal endophytes were isolated from a young heathy branch of Chinese fir and screened against the pathogen in vitro. One isolate, designated as SMEL1 and subsequently identified as Epicoccum dendrobii based on morphological and phylogenetic analyses, suppressed mycelial growth of C. gloeosporioides on dual culture plates. Additionally, the metabolites of E. dendrobii significantly decreased the biomass of C. gloeosporioides. E. dendrobii was able to enter the internal tissues of the host plant via stomatal cells. The metabolites of E. dendrobii significantly inhibited conidial germination and appressorium formation, which at least partly explained why the endophyte significantly inhibited lesion development caused by C. gloeosporioides on various host plants. We further confirmed that some components with antifungal activity could be extracted from E. dendrobii using ethyl acetate as an organic solvent. To our knowledge, this is the first report of E. dendrobii as a potential biocontrol agent against a fungal phytopathogen.

scholarly journals Cladobotryum mycophilum as Potential Biocontrol Agent

Agronomy ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 891
Author(s):  
Mila Santos ◽  
Fernando Diánez ◽  
Alejandro Moreno-Gavíra ◽  
Brenda Sánchez-Montesinos ◽  
Francisco J. Gea
Keyword(s):  
Biological Control ◽  
Fusarium Oxysporum ◽  
Biocontrol Agent ◽  
Biological Control Agent ◽  
Control Agent ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Dual Culture ◽  
Potential Biocontrol Agent

A study was conducted to explore the efficacy of potential biocontrol agent Cladobotryum mycophilum against different phytopathogenic fungi. The growth rates of 24 isolates of C. mycophilum were determined, and their antagonistic activity was analysed in vitro and in vivo against Botrytis cinerea, Fusarium oxysporum f. sp. radicis-lycopersici, Fusarium oxysporum f.sp. cucumerinum, Fusarium solani, Phytophthora parasitica, Phytophthora capsici, Pythium aphanidermatum and Mycosphaerella melonis. Most isolates grow rapidly, reaching the opposite end of the Petri dish within 72–96 h. Under dual-culture assays, C. mycophilum showed antagonistic activity in vitro against all phytopathogenic fungi tested, with mycelial growth inhibition ranging from 30 to 90% against all the different phytopathogens tested. Similarly, of all the selected isolates, CL60A, CL17A and CL18A significantly (p < 0.05) reduced the disease incidence and severity in the plant assays compared to the controls for the different pathosystems studied. Based on these results, we conclude that C. mycophilum can be considered as a potential biological control agent in agriculture. This is the first study of Cladobotryum mycophilum as a biological control agent for different diseases caused by highly relevant phytopathogens in horticulture.

In vitro biological management of Anthracnose disease in Morinda citrifolia Linn.

2019 ◽  
Vol 26 (3) ◽  
pp. 137-139
Author(s):  
P. Swetha ◽  
◽  
S. Renuka ◽  
R. Sundararaj ◽  
◽  
...  
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Biocontrol Agent ◽  
Antagonistic Effect ◽  
Morinda Citrifolia ◽  
Trichoderma Virens ◽  
Anthracnose Disease ◽  
Potential Biocontrol Agent ◽  
The Family ◽  
Indian Mulberry

Morinda citrifolia Linn., also called as Indian mulberry or Noni is a widely used medicinal plant belonging to the family, Rubiaceae. It suffers from severe diseases which hinder the healing properties of the plant. Hence, there is a significant need to prevent and manage the diseases which the plants are prone to. In this paper, we are reporting the in vitro biological control of Anthracnose disease caused by the pathogen Colletotrichum gloeosporioides (Penz.) Penz. & Sacc. in M. citrifolia. The identity of the pathogen was confirmed through microscopic observation and molecular characterization. Furthermore, an in-vitro bioassay was carried out to study the antagonistic effect of Trichoderma virens (J.H. Mill., Giddens & A.A. Foster) Arx. against C. gloeosporioides and the result showed 72.60 % inhibition of C. gloeosporioides by T. virens. Hence, T. virens can be recommended as a potential biocontrol agent against C. gloeosporioides causing Anthracnose disease of M. citrifolia.

scholarly journals Two new species of Undifilum, fungal endophytes of Astragalus (locoweeds) in the United States

Botany ◽  
2012 ◽  
Vol 90 (9) ◽  
pp. 866-875 ◽  
Author(s):  
Deana L. Baucom ◽  
Marie Romero ◽  
Robert Belfon ◽  
Rebecca Creamer
Keyword(s):  
New Species ◽  
New Mexico ◽  
Random Amplified Polymorphic Dna ◽  
Phylogenetic Analyses ◽  
Fungal Endophytes ◽  
Morphological Characteristics ◽  
Its Region ◽  
The United States ◽  
Neighbor Joining

New species of Undifilum , from locoweeds Astragalus lentiginosus Vitman and Astragalus mollissimus Torr., are described using morphological characteristics and molecular phylogenetic analyses as Undifilum fulvum Baucom & Creamer sp. nov. and Undifilum cinereum Baucom & Creamer sp. nov. Fungi were isolated from dried plants of A. lentiginosus var. araneosus , diphysus , lentiginosus , and wahweapensis collected from Arizona, Oregon, and Utah, USA, and A. mollissimus var. biglovii , earleii , and mollissimus collected from New Mexico, Oklahoma, and Texas, USA. Endophytic fungi from Astragalus locoweeds were compared to Undifilum oxytropis isolates obtained from dried plant material of Oxytropis lamberteii from New Mexico and Oxytropis sericea from Arizona, Colorado, New Mexico, Utah, and Wyoming. Extremely slow growth in vitro was observed for all, and conidia, if present, were ellipsoid with transverse septa. However, in vitro color, growth on four different media, and conidium size differed between fungi from Astragalus spp. and U. oxytropis. Neighbor-joining analyses of internal transcribed spacer (ITS) region and glyceraldehyde-3-phosphate dehydrogenase (GPD) gene sequences revealed that U. fulvum and U. cinereum formed a clade distinct from U. oxytropis. This was supported by neighbor-joining analyses of results generated from random amplified polymorphic DNA (RAPD) fragments using two different primers.

scholarly journals Effects of post-emergent herbicides on Trichoderma harzianum , a potential biocontrol agent against Sclerotinia sclerotiorum in soybean cropping

2007 ◽  
Vol 55 (3) ◽  
pp. 355-362
Author(s):  
M. Rollán ◽  
C. Mónaco ◽  
G. Lampugnani ◽  
N. Arteta ◽  
D. Bayo ◽  
...  
Keyword(s):  
Sclerotinia Sclerotiorum ◽  
Trichoderma Harzianum ◽  
Mycelial Growth ◽  
Detrimental Effect ◽  
Biocontrol Agent ◽  
Selective Medium ◽  
Potential Biocontrol Agent ◽  
Colony Forming Units ◽  
Soil Dilution

Trichoderma harzianum is a potential biocontrol agent against Sclerotinia sclerotiorum in soybean. Information is needed on the compatibility of this biocontrol agent and the post-emergent herbicides used in soybean cropping.Haloxyfop R Methyl (EC 10.4%), Glyphosate (SL 48%), Imazamox (WG 70%) and Imazethapyr (SL 10%) were evaluated for their effects on the mycelial growth of T. harzianum on in vitro agar plates. Glyphosate (2000 ppm), Imazethapyr (500 and 250 ppm) and Haloxyfop R Methyl (1000, 500 and 100 ppm) reduced the mycelial growth of T. harzianum . Imazamox had no effect at any concentration.Subsequently, all the herbicides were assessed for their effect on soil populations of T. harzianum . Greenhouse assays conducted with non-sterile soil inoculated with T. harzianum and a specific herbicide were sampled before pesticide application and after 30 days. The number of colony forming units per gram of soil (c.f.u./g of soil) was evaluated with a soil dilution technique using Trichoderma selective medium (TSM). No detrimental effect was revealed.

scholarly journals Antibacterial, Antibiofilm, and Antioxidant Activity of Polysaccharides Obtained from Fresh Sarcotesta of Ginkgo biloba: Bioactive Polysaccharide that Can Be Exploited as a Novel Biocontrol Agent

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Hong Jiang ◽  
Zuxiang Luan ◽  
Zhaobing Fan ◽  
Xinliang Wu ◽  
Ziheng Xu ◽  
...  
Keyword(s):  
Ginkgo Biloba ◽  
High Performance ◽  
Biocontrol Agent ◽  
Glucuronic Acid ◽  
Antibacterial Effect ◽  
Natural Antioxidants ◽  
Potential Biocontrol Agent ◽  
Polysaccharide Intercellular Adhesion ◽  
Icad Gene

Staphylococcus aureus (S. aureus) biofilm plays an important role in the persistence of chronic infection due to its resistance to antibiotics. Because of their functional diversity, active polysaccharide is increasingly being applied as a biocontrol agent to inhibit the formation of biofilm by pathogens. In this study, a new polysaccharide, GBSPII-1, isolated from the fresh sarcotesta of Ginkgo biloba L. (G. biloba) was characterized and its effect on antibiofilm formation of S. aureus was examined in vitro. High-Performance Liquid Chromatography (HPLC) analysis showed that GBSPII-1 is an acidic heteropolysaccharide composed of mannose, rhamnose, glucose, glucuronic acid, and galacturonic acid. GBSPII-1 demonstrated a molecular weight of 34 kDa and may affect the accumulation of polysaccharide intercellular adhesion (PIA) by inhibiting icaA, icaB, icaC, and icaD gene expression at subinhibitory concentrations. Under 10 g/L, GBSPII-1 showed an antioxidant effect on the inhibition rate of H2O2-induced erythrocyte hemolysis and the scavenging rate of DPPH radicals was 76.5 ± 0.5% and 89.2 ± 0.26%, respectively. The findings obtained in this study indicate that GBSPII-1 has antibacterial effect, is a possible source of natural antioxidants, and may be a potential biocontrol agent for the design of new therapeutic strategies for biofilm-related S. aureus infections.

scholarly journals Antagonisme Fungi Endofit Daun Jarak Pagar (Jatropha curcas) terhadap Fusarium oxysporum C2 Penyebab Busuk Umbi pada Bawang Merah in Vitro

2020 ◽  
Vol 4 (1) ◽  
pp. 47
Author(s):  
Fauziyyah Nahdah ◽  
Noorkomala Sari ◽  
Akhmad Rizali ◽  
Rabiatul Wahdah
Keyword(s):  
Fusarium Oxysporum ◽  
Jatropha Curcas ◽  
Linear Trend ◽  
Culture Method ◽  
Fungal Endophyte ◽  
Dual Culture ◽  
Antimicrobial Substances ◽  
Spss Software

<p class="Abstract">Basal plate rot is a major disease on shallot caused by <em>Fusarium oxysporum</em>. Endophytic fungi is promising to use as antagonist agent to the pathogen. Endophyte is microbes that are living in plant cells and have an asymptomatic characteristic. Nowadays, fungal endophyte is believed to produce antimicrobial substances similar with their plant host's natural product. <em>Jatropha curcas</em> is one of the plants containing secondary metabolites that have antifungal activities. The research aimed to study the ability of endophyte from <em>Jatropha curcas</em> to inhibit the growth of <em>Fusarium oxysporum</em>. The dual culture method was used in this research and the data were analyzed by SPSS software. This antagonism test was conducted by 9 isolates endophyte and each plate consisted of 3 replicates. The result revealed endophyte fungal obtaining 9 isolates with the radial growth of 4,5 cm/2 days. Endophytes of <em>Jatropha curcas</em> L. were able to inhibit the growth of <em>Fusarium oxysporum</em> C2. The percentage of inhibition of <em>Fusarium oxysporum </em>causing of root blight diseases was controlled by up 38.27 - 74.48%. The highest percentage of inhibition is gained by B4b and the lowest of it is A2b. Our observations showed that each endophyte has a consistent linear trend. B4b still leaded as the highest strength to inhibit the growth of pathogen on the monitoring of 3, 5, and 7 days. Moreover, the ability of fungi endophyte from <em>Jatropha curcas</em> as antagonist agent to <em>Fusarium oxysporum</em> needs to be further examined by the in vivo method.</p>

scholarly journals PEMANFAATAN KITOSAN DAN Trichoderma sp. Rifai. UNTUK MENGENDALIKAN PENYAKIT ANTRAKNOSA (Colletotrichum gloeosporioides Penz.) PADA BUAH PISANG CAVENDISH

2014 ◽  
Vol 2 (2) ◽  
Author(s):  
Fikriyah Shofiah Mawaddah ◽  
Joko Prasetyo ◽  
Muhammad Nurdin
Keyword(s):  
Colletotrichum Gloeosporioides ◽  
Culture Method ◽  
Dual Culture ◽  
Trichoderma Sp

Antraknosa yang disebabkan oleh Colletotrichum gloeosporioides merupakan penyakit pascapanen penting pada buahbuahan. Penelitian ini bertujuan untuk mengetahui efektifitas kitosan dan Trichoderma sp. dalam menghambat pertumbuhan koloni jamur C. gloeosporioides secara in vitro dan mengetahui efektifitas kitosan dan Trichoderma sp. terhadap intensitas penyakit antraknosa yang disebabkan oleh C. gloeosporioides pada buah pisang cavendish. Penelitian dilakukan di Laboratorium Penyakit Tumbuhan, Fakultas Pertanian, Universitas Lampung dari bulan Mei 2013 sampai dengan September 2013. Percobaan ini disusun dalam rancangan acak lengkap (RAL) yang terdiri atas lima perlakuan dan lima ulangan, yaitu kontrol (P0), kitosan (P1), Trichoderma sp. (P2), kombinasi kitosan dan Trichoderma sp. (P3) dan fungisida mankozeb (P4). C. gloeosporioides diperoleh dari isolasi buah pisang yang bergejala antraknosa. Pengujian secara in vitro, perlakuan kitosan konsentrasi 0,75% dicampurkan ke dalam media potato sukrose agar, perlakuan Trichoderma sp. (dual culture method), perlakuan kombinasi kitosan dan Trichoderma sp. dengan mencampurkan kitosan pada media dan dikombinasikan dengan Trichoderma sp. (dual culture method) serta perlakuan fungisida mankozeb konsentrasi 0,1% dicampurkan ke dalam media. Metode tersebut juga digunakan untuk pengujian secara in vivo pada buah pisang. Hasil pengujian secara in vitro, perlakuan kitosan, Trichoderma sp. dan kombinasi kitosan dan Trichoderma sp. efektif menghambat pertumbuhan koloni jamur C. gloeosporioides. Pada uji in vivo, masing-masing perlakuan tidak efektif menghambat intensitas penyakit antraknosa.

scholarly journals Novel Antifungal Activity of Lolium-Associated Epichloë Endophytes

2020 ◽  
Vol 8 (6) ◽  
pp. 955 ◽  
Author(s):  
Krishni Fernando ◽  
Priyanka Reddy ◽  
Inoka K. Hettiarachchige ◽  
German C. Spangenberg ◽  
Simone J. Rochfort ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Insect Pests ◽  
Fungal Endophytes ◽  
Dual Culture ◽  
The Novel ◽  
In Planta ◽  
Pasture Production ◽  
Fusarium Sp ◽  
Invertebrate Pests

Asexual Epichloë spp. fungal endophytes have been extensively studied for their functional secondary metabolite production. Historically, research mostly focused on understanding toxicity of endophyte-derived compounds on grazing livestock. However, endophyte-derived compounds also provide protection against invertebrate pests, disease, and other environmental stresses, which is important for ensuring yield and persistence of pastures. A preliminary screen of 30 strains using an in vitro dual culture bioassay identified 18 endophyte strains with antifungal activity. The novel strains NEA12, NEA21, and NEA23 were selected for further investigation as they are also known to produce alkaloids associated with protection against insect pests. Antifungal activity of selected endophyte strains was confirmed against three grass pathogens, Ceratobasidium sp., Dreschlera sp., and Fusarium sp., using independent isolates in an in vitro bioassay. NEA21 and NEA23 showed potent activity against Ceratobasidium sp. and NEA12 showed moderate inhibition against all three pathogens. Crude extracts from liquid cultures of NEA12 and NEA23 also inhibited growth of the phytopathogens Ceratobasidium sp. and Fusarium sp. and provided evidence that the compounds of interest are stable, constitutively expressed, and secreted. Comparative analysis of the in vitro and in planta metabolome of NEA12 and NEA23 using LCMS profile data revealed individual metabolites unique to each strain that are present in vitro and in planta. These compounds are the best candidates for the differential bioactivity observed for each strain. Novel endophyte strains show promise for endophyte-mediated control of phytopathogens impacting Lolium spp. pasture production and animal welfare.

scholarly journals Trichoderma species as Biocontrol Agent against Soil Borne Fungal Pathogens

2017 ◽  
Vol 5 (1) ◽  
pp. 39-45 ◽  
Author(s):  
Srijana Bastakoti ◽  
Shiva Belbase ◽  
Shrinkhala Manandhar ◽  
Charu Arjyal
Keyword(s):  
Rhizoctonia Solani ◽  
Fusarium Solani ◽  
Fungal Pathogens ◽  
Plant Pathogens ◽  
Biocontrol Agent ◽  
Sclerotium Rolfsii ◽  
Dual Culture ◽  
Trichoderma Species ◽  
Biocontrol Efficacy

Soil borne pathogenic fungi are of major concern in agriculture which significantly decreases the plant yield. Chemically controlled plant imposes environmental threats potentially dangerous to humans as well as other animals. Thus, application of biological methods in plant disease control is more effective alternative technique. This study was carried out to isolate Trichoderma species from soil sample and to assess its in vitro biocontrol efficacy against fungal pathogens viz. Sclerotium rolfsii, Sclerotionia sclerotiorum, Fusarium solani and Rhizoctonia solani. Biocontrol efficacy testing of isolates against different fungal pathogens was performed by dual culture technique.In this study, 5 different Trichoderma species were isolated from 26 various soil samples and were tested against four fungal soil-borne pathogens. Inhibition percentage of radial growth of Sclerotium rolfsii by three of the Trichoderma isolates was found to be 100%; about 62% and 68% of maximum inhibition was observed against Rhizoctonia solani and Fusarium solani respectively whereas Sclerotionia sclerotiorum was inhibited maximum up to 23%. This in vitro study revealed that although Trichoderma species plays an important role in controlling all type of soil borne fungal plant pathogens, however, isolates as biocontrol agent against Sclerotium rolfsii was found to be more efficient in comparison to other pathogens.Nepal Journal of Biotechnology. Dec. 2017 Vol. 5, No. 1: 39-49

Endophytic fungi as a promising biocontrol agent to protect wheat from Fusarium graminearum head blight

Plant Disease ◽  
2021 ◽  
Author(s):  
Zachary Albert Noel ◽  
Ludmilla Roze ◽  
Mikaela Breunig ◽  
Frances Trail
Keyword(s):  
Microbial Community ◽  
Fusarium Graminearum ◽  
Endophytic Fungi ◽  
Seed Weight ◽  
Biocontrol Agent ◽  
Fungal Endophytes ◽  
In Planta ◽  
Head Blight ◽  
Species Definitions

The search for beneficial endophytes that can be part of a constructed microbial community has increased in recent years. We characterized three endophytic fungi previously isolated from wheat for their in vitro and in planta antagonism toward the Fusarium head blight pathogen, Fusarium graminearum. The endophytes were phylogenetically characterized and shown to be Alternaria destruens, Fusarium commune, and Fusarium oxysporum. Individual fungal endophytes significantly increased seed weight and lowered the accumulation of the mycotoxin deoxynivalenol compared to F. graminearum infected wheat heads without endophyte pretreatment. Investigation into the mechanism of competition in vitro showed that endophytes competitively excluded F. graminearum by pre-emptive colonization and possible inhibition over a distance. Investigations on the use of these endophytes in the field are in progress. Identification of these three endophytes highlights a common quandary in searching for beneficial microbes to use in agriculture: species definitions often do not separate individual isolates’ lifestyles. A greater understanding of the risks in using intraspecies variants for biocontrol is needed and should be examined in the context of the ecology of the individuals being investigated.

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