Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Enterobacter Ludwigii ◽  
Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

scholarly journals Characterisation of Plant Growth-Promoting Endophytic Bacteria from Sugarcane and Their Antagonistic Activity against Fusarium moniliforme

2021 ◽  
Vol 32 (3) ◽  
pp. 97-118
Author(s):  
Nittaya Pitiwittayakul ◽  
Duanpen Wongsorn ◽  
Somboon Tanasupawat
Keyword(s):  
Plant Growth ◽  
Endophytic Bacteria ◽  
Fusarium Moniliforme ◽  
Growth Promotion ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Burkholderia Gladioli ◽  
Plant Growth Promoting ◽  
Chemical Usage

The use of endophytic bacteria in agriculture provides an effective way of improving crop yield and significantly reducing chemical usage, such as fungicides. This research was conducted to explore endophytic bacteria with plant growth promotion (PGP) and antifungal activities against Fusarium moniliforme AIT01. In this study, we obtained 52 isolates of endophytic bacteria associated with the roots and stems of sugarcane from Nakhon Ratchasima province, Thailand. In vitro antagonistic activity test showed that 14 out of 52 isolates had antagonistic activity against the fungal pathogen F. moniliforme AIT01. These antagonistic endophytic bacteria were identified as belonging to six different species as follows: Nguyenibacter vanlangensis, Acidomonas methanolica, Asaia bogorensis, Tanticharoenia aidae, Burkholderia gladioli and Bacillus altitudinis based on phenotypic characteristics, along with phylogenetic analysis of their 16S rRNA gene sequences. Seven isolates effectively inhibited F. moniliforme AIT01 mycelial growth by up to 40%. The volatile compounds of six isolates reduced the growth of F. moniliforme AIT01 by over 23%. Moreover, riceberry rice seedlings previously treated with B. gladioli CP28 were found to strongly reduce infection with phytopathogen by 80% in comparison to the non-treated control. Furthermore, the isolates also showed relevant PGP features, including ammonia production, zinc and phosphate solubilisation, auxin and siderophore biosynthesis. These results demonstrated that the tested endophytic bacteria could be successfully utilised as a source of PGP and biocontrol agent to manage diseases caused by F. moniliforme.

scholarly journals Arsenic-Redox Transformation and Plant Growth Promotion by Purple Nonsulfur Bacteria Rhodopseudomonas palustris CS2 and Rhodopseudomonas faecalis SS5

2017 ◽  
Vol 2017 ◽  
pp. 1-8 ◽  
Author(s):  
Kanza Batool ◽  
Fatima tuz Zahra ◽  
Yasir Rehman
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Length ◽  
Growth Promotion ◽  
Rhodopseudomonas Palustris ◽  
Shoot Length ◽  
Rrna Gene ◽  
Purple Nonsulfur Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

Arsenic (As) is a well-known toxic metalloid found naturally and released by different industries, especially in developing countries. Purple nonsulfur bacteria (PNSB) are known for wastewater treatment and plant growth promoting abilities. As-resistant PNSB were isolated from a fish pond. Based on As-resistance and plant growth promoting attributes, 2 isolates CS2 and SS5 were selected and identified as Rhodopseudomonas palustris and Rhodopseudomonas faecalis, respectively, through 16S rRNA gene sequencing. Maximum As(V) resistance shown by R. faecalis SS5 and R. palustris CS2 was up to 150 and 100 mM, respectively. R. palustris CS2 showed highest As(V) reduction up to 62.9% (6.29±0.24 mM), while R. faecalis SS5 showed maximum As(III) oxidation up to 96% (4.8±0.32 mM), respectively. Highest auxin production was observed by R. palustris CS2 and R. faecalis SS, up to 77.18±3.7 and 76.67±2.8 μg mL−1, respectively. Effects of these PNSB were tested on the growth of Vigna mungo plants. A statistically significant increase in growth was observed in plants inoculated with isolates compared to uninoculated plants, both in presence and in absence of As. R. palustris CS2 treated plants showed 17% (28.1±0.87 cm) increase in shoot length and 21.7% (7.07±0.42 cm) increase in root length, whereas R. faecalis SS5 treated plants showed 12.8% (27.09±0.81 cm) increase in shoot length and 18.8% (6.9±0.34 cm) increase in root length as compared to the control plants. In presence of As, R. palustris CS2 increased shoot length up to 26.3% (21.0±1.1 cm), while root length increased up to 31.3% (5.3±0.4 cm), whereas R. faecalis SS5 inoculated plants showed 25% (20.7 ± 1.4 cm) increase in shoot length and 33.3% (5.4 ± 0.65 cm) increase in root length as compared to the control plants. Bacteria with such diverse abilities could be ideal for plant growth promotion in As-contaminated sites.

Characterization of Mesorhizobium strains for salt tolerance and wilt control: Their potential for plant growth promotion of chickpea (Cicer arietinum L.)

2018 ◽  
Author(s):  
Anju Sehrawat ◽  
Aakanksha Khandelwal ◽  
Satyavir Singh Sindhu
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Effect ◽  
Maximum Increase ◽  
Shoot Dry Weight ◽  
Plant Growth Promoting

Mesorhizobium sp. indirectly promote the growth of plants as a biocontrol agent by inhibiting the growth of pathogens particularly Fusarium wilt of chickpea. Out of 24 Mesorhizobium isolates obtained from chickpea nodules, eight isolates showed antagonistic effect against Fusarium oxysporum. Salinity stress severely affects growth, nodulation and yield of chickpea. Mesorhizobium isolates were tested for their salt tolerance capacity at 1, 2, 4, 6 and 8% NaCl concentrations. Only two Mesorhizobium isolates MCA5 and MCA22 were found salt-tolerant upto 8% of salt concentration. Maximum increase (45.5%) in shoot dry weight was observed by inoculation of isolate MCA20 at 40 days of chickpea growth under chillum jar conditions, whereas isolate MCA23 resulted in 166.2% increase in root dry weight. Likewise, 112.6% increase in shoot dry weight was observed on inoculation of MCA14 isolate at 80th day of observation. Further extensive research is required to understand the mechanism of potential Mesorhizobium isolates of chickpea in controlling Fusarium wilt disease and salt tolerance. Selection of mesorhizobia with twin functional traits (plant growth promoting and biocontrol agent) can be exploited as future biofertilizer in chickpea.

scholarly journals Bacteria Isolated from Wastewater Irrigated Agricultural Soils Adapt to Heavy Metal Toxicity While Maintaining Their Plant Growth Promoting Traits

2021 ◽  
Vol 13 (14) ◽  
pp. 7792
Author(s):  
Abdul Wahab Ajmal ◽  
Saleha Saroosh ◽  
Shah Mulk ◽  
Muhammad Nadeem Hassan ◽  
Humaira Yasmin ◽  
...  
Keyword(s):  
Plant Growth ◽  
Agricultural Soils ◽  
Culture Media ◽  
Growth Promotion ◽  
Metal Resistance ◽  
Rrna Gene ◽  
Gene Sequence Analysis ◽  
Plant Growth Promoting ◽  
Growth Promoting

The present study explored the plant growth promotion and bioremediation potential of bacteria inhabiting wastewater irrigated agricultural soils. Thirty out of 75 bacterial isolates (40%), 29/75 (39%) and 28/75 (37%) solubilized Zn, K and PO4 during plate essays respectively. Fifty-six percent of the isolates produced siderophores, while 30% released protease in vitro. Seventy-four percent of bacteria resisted Pb, Ni and Cd at various concentrations added to the culture media plates. Sixteen out of 75 (26%) isolates were able to fix N in Nbf medium. Among these 16 N fixers, N fixing nifH, nifD and nifK genes was detected through PCR in 8, 7 and 1 strain respectively using gene specific primers designed in the study with Enterobacter sp. having all three (nifHKD) genes. Isolated bacteria showed resemblance to diverse genera such as Bacillus, Pseudomonas, Enterobacter, Citrobacter, Acinetobacter, Serratia, Klebsiella and Enterococcus based on 16S rRNA gene sequence analysis. In addition to showing the best mineral solubilization and metal resistance potential, Citrobacter sp. and Enterobacter sp. also removed 87%, 79% and 43% and 86%, 78% and 51% of Ni, Cd and Pb, respectively, from aqueous solution. These potent bacteria may be exploited both for bioremediation and biofertilization of wastewater irrigated soils leading to sustainable agriculture.

scholarly journals Genomic and Metabolomic Insights into Secondary Metabolites of the Novel Bacillus halotolerans Hil4, an Endophyte with Promising Antagonistic Activity against Gray Mold and Plant Growth Promoting Potential

2021 ◽  
Vol 9 (12) ◽  
pp. 2508
Author(s):  
Eirini-Evangelia Thomloudi ◽  
Polina C. Tsalgatidou ◽  
Eirini Baira ◽  
Konstantinos Papadimitriou ◽  
Anastasia Venieraki ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Gene Clusters ◽  
Gray Mold ◽  
Antagonistic Activity ◽  
Future Research ◽  
Single Culture ◽  
Plant Growth Promoting ◽  
Growth Promoting

The endophytic bacterial strain Hil4 was isolated from leaves of the medicinal plant Hypericum hircinum. It exhibited antifungal activity against Botrytis cinerea and a plethora of plant growth promoting traits in vitro. Whole genome sequencing revealed that it belongs to Bacillus halotolerans and possesses numerous secondary metabolite biosynthetic gene clusters and genes involved in plant growth promotion, colonization, and plant defense elicitation. The Mojavensin cluster was present in the genome, making this strain novel among plant-associated B. halotolerans strains. Extracts of secreted agar-diffusible compounds from single culture secretome extracts and dual cultures with B. cinerea were bioactive and had the same antifungal pattern on TLC plates after bioautography. UHPLC-HRMS analysis of the single culture secretome extract putatively annotated the consecutively produced antimicrobial substances and ISR elicitors. The isolate also proved efficient in minimizing the severity of gray mold post-harvest disease on table grape berries, as well as cherry tomatoes. Finally, it positively influenced the growth of Arabidopsis thaliana Col-0 and Solanum lycopersicum var. Chondrokatsari Messinias after seed biopriming in vitro. Overall, these results indicate that the B. halotolerans strain Hil4 is a promising novel plant growth promoting and biocontrol agent, and can be used in future research for the development of biostimulants and/or biological control agents.

scholarly journals Genome-guided insights of tropical Bacillus strains efficient in maize growth promotion

2020 ◽  
Vol 96 (9) ◽  
Author(s):  
Camila Cristina Vieira Velloso ◽  
Christiane Abreu de Oliveira ◽  
Eliane Aparecida Gomes ◽  
Ubiraci Gomes de Paula Lana ◽  
Chainheny Gomes de Carvalho ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Dry Weight ◽  
Phenotypic Characterization ◽  
Plant Growth Promoting Bacteria ◽  
Maize Genotypes ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Bacillus Strains

ABSTRACT Plant growth promoting bacteria (PGPB) are an efficient and sustainable alternative to mitigate biotic and abiotic stresses in maize. This work aimed to sequence the genome of two Bacillus strains (B116 and B119) and to evaluate their plant growth-promoting (PGP) potential in vitro and their capacity to trigger specific responses in different maize genotypes. Analysis of the genomic sequences revealed the presence of genes related to PGP activities. Both strains were able to produce biofilm and exopolysaccharides, and solubilize phosphate. The strain B119 produced higher amounts of IAA-like molecules and phytase, whereas B116 was capable to produce more acid phosphatase. Maize seedlings inoculated with either strains were submitted to polyethylene glycol-induced osmotic stress and showed an increase of thicker roots, which resulted in a higher root dry weight. The inoculation also increased the total dry weight and modified the root morphology of 16 out of 21 maize genotypes, indicating that the bacteria triggered specific responses depending on plant genotype background. Maize root remodeling was related to growth promotion mechanisms found in genomic prediction and confirmed by in vitro analysis. Overall, the genomic and phenotypic characterization brought new insights to the mechanisms of PGP in tropical Bacillus.

scholarly journals Rice-Associated Rhizobacteria as a Source of Secondary Metabolites against Burkholderia glumae

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2567
Author(s):  
Giann Carlos Peñaloza Atuesta ◽  
Walter Murillo Arango ◽  
Jordi Eras ◽  
Diego Fernándo Oliveros ◽  
Jonh Jairo Méndez Arteaga
Keyword(s):  
Growth Promotion ◽  
Phylogenetic Analyses ◽  
Inorganic Phosphorus ◽  
Antagonistic Activity ◽  
Diffusion Method ◽  
Rrna Gene ◽  
Growth Promoters ◽  
Burkholderia Glumae ◽  
Main Compound

Various diseases, including bacterial panicle blight (BPB) and sheath rot, threaten rice production. It has been established that Burkholderia glumae (B. glumae) is the causative agent of the above mentioned pathologies. In the present study, antagonistic activity, growth promotion, and the metabolite profiles of two rhizobacteria, isolated in different paddy fields, were assessed against B. glumae. Strains were identified based on 16S rRNA gene sequences, and the phylogenetic analyses showed that both strains belong to the genus Enterobacter, with high similarity to the strain Enterobacter tabaci NR146667.2 (99%). The antagonistic activity was assessed with the disc diffusion method. Active fractions were isolated through a liquid/liquid extraction with ethyl acetate (EtOAc) from the fermentation media, and their antibacterial activities were evaluated following the Clinical and Laboratory Standards Institute (CLSI) guidelines. The Pikovskaya modified medium was used to test the ability of in vitro inorganic phosphorus solubilization, and BSB1 proved to be the best inorganic phosphorus solubilizer, with a solubilization index (SI) of 4.5 ± 0.2. The glass-column fractionation of the EtOAc extracted from BCB11 produced an active fraction (25.9 mg) that inhibited the growth of five B. glumae strains by 85–95%. Further, metabolomic analysis, based on GC–MS, showed 3-phenylpropanoic acid (3-PPA) to be the main compound both in this fraction (46.7%), and in the BSB1 extract (28.6%). This compound showed antibacterial activity against all five strains of B. glumae with a minimum inhibitory concentration (MIC) of 1000 mg/L towards all of them. The results showed that rice rhizosphere microorganisms are a source of compounds that inhibit B. glumae growth and are promising plant growth promoters (PGP).

scholarly journals Isolation of plant growth-promoting Pseudomonas sp. PPR8 from the rhizosphere of Phaseolus vulgaris L.

2016 ◽  
Vol 68 (2) ◽  
pp. 363-374 ◽  
Author(s):  
Pankaj Kumar ◽  
Ramesh Dubey ◽  
Dinesh Maheshwari ◽  
Yong-Ha Park ◽  
Vivek Bajpai
Keyword(s):  
Phaseolus Vulgaris ◽  
Plant Growth ◽  
Growth Promotion ◽  
Rrna Gene ◽  
Sequencing Analysis ◽  
Pseudomonas Sp ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

In vitro screening of plant growth-promoting (PGP) traits was carried out using eight Pseudomonas spp., PPR1 to PPR8, isolated from the rhizosphere of Phaseolus vulgaris growing on the Uttarakhand Himalayan range in India. All the isolates were fast growers, positive for catalase, oxidase and urease activities, and utilized lactose and some amino acids. All the isolates were indole acetic acid (IAA) positive, however PPR8 solubilized potassium and zinc along with various other types of inorganic (tricalcium, dicalcium and zinc phosphate) and organic (calcium phytate) phosphates, as well as producing siderophore and ACC deaminase. PPR8 also produced cyanogens, extracellular chitinase, ?-1,3-glucanase, ?-1,4-glucanase and oxalate oxidase. Based on the PGP traits of all isolates, PPR8 was found to be the most potent plant growth-promoting rhizobacteria (PGPR). Further, PPR8 was identified as Pseudomonas sp. PPR8, based on 16S rRNA gene sequencing analysis. Moreover, the PGP activities of PPR8 confirmed it to be a potent biocontrol agent, inhibiting the growth of various plant pathogenic fungi. This study reveals the potential of Pseudomonas sp. PPR8 to be used as a good bioinoculant for growth promotion of common bean and for the protection of important legume crops from various deleterious phytopathogens.

scholarly journals In vitro Evaluation of Bacterial Endophytes for Biocontrol of Pythium aphanidermatum and Plant Growth Promotion in Setaria italica L. Grown in Seedling Trays

2019 ◽  
pp. 1-14 ◽  
Author(s):  
M. Raveendra Reddy ◽  
M. K. Shivaprakash ◽  
S. Adithya
Keyword(s):  
Growth Promotion ◽  
Disease Incidence ◽  
Foxtail Millet ◽  
Dry Weight ◽  
Pythium Aphanidermatum ◽  
Antagonistic Activity ◽  
Setaria Italica ◽  
Bacterial Endophytes ◽  
Causal Organism

An investigation study was carried to test the ability of endophytic bacteria isolated from small millets as a biocontrol agent against the fungal pathogen Pythium aphanidermatum, the causal organism of sheath blight in foxtail millet (Setaria italica L.) grown in seedling trays under greenhouse conditions. In total twelve bacterial endophytes were isolated out of which six isolates produced β -1, 3 glucanases, 11 isolates produced chitinases and 12 isolates produced siderophores under in-vitro conditions. All these bacterial endophytic isolates inhibited mycelial growth of Pythium aphanidermatum in plate assay and liquid culture and the highest percent inhibition of mycelium was recorded in KMS5 (44.44%) followed by KMS1 (38.89%). In seedling trays, BMR7 and KMS5 (81.66%) had recorded the highest percent germination followed by KMS1 (79.62%) and KMS1 and KMS5 took 10.85 and 10.55 days respectively for 50% seed germination compared to control (13.50 days) which was treated with pathogen alone. Lowest pre-emergence disease incidence was noticed in KMS5 (14.03%) followed by KMS1 (16.18%) whereas T1 (Control) recorded maximum pre-emergence disease incidence (39.82%) was observed. Least post-emergence disease incidence (11.36) and biocontrol efficacy (68.74%) was recorded with KMS5. Apart from showing antagonistic activity, KMS5 had recorded a significantly higher seedling vigor index (2712.97), root length (18.12 cm), shoot length (15.10 cm), root dry weight (0.158 g) and shoot dry weight (0.76 g) compared to other bacterial endophytic isolates.

scholarly journals Endophytic Bacterial Isolates From Halophytes Demonstrate Phytopathogen Biocontrol and Plant Growth Promotion Under High Salinity

2021 ◽  
Vol 12 ◽  
Author(s):  
Christos A. Christakis ◽  
Georgia Daskalogiannis ◽  
Anastasia Chatzaki ◽  
Emmanouil A. Markakis ◽  
Glykeria Mermigka ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
High Salinity ◽  
Growth Promotion ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
In Planta ◽  
Promoting Effect ◽  
Cakile Maritima

Halophytic endophytes potentially contribute to the host’s adaptation to adverse environments, improving its tolerance against various biotic and abiotic stresses. Here, we identified the culturable endophytic bacteria of three crop wild relative (CWR) halophytes: Cakile maritima, Matthiola tricuspidata, and Crithmum maritimum. In the present study, the potential of these isolates to improve crop adaptations to various stresses was investigated, using both in vitro and in-planta approaches. Endophytic isolates were identified by their 16S rRNA gene sequence and evaluated for their ability to: grow in vitro in high levels of NaCl; inhibit the growth of the economically important phytopathogens Verticillium dahliae, Ralstonia solanacearum, and Clavibacter michiganensis and the human pathogen Aspergillus fumigatus; provide salt tolerance in-planta; and provide growth promoting effect in-planta. Genomes of selected isolates were sequenced. In total, 115 endophytic isolates were identified. At least 16 isolates demonstrated growth under increased salinity, plant growth promotion and phytopathogen antagonistic activity. Three showed in-planta suppression of Verticillium growth. Furthermore, representatives of three novel species were identified: two Pseudomonas species and one Arthrobacter. This study provides proof-of-concept that the endophytes from CWR halophytes can be used as “bio-inoculants,” for the enhancement of growth and stress tolerance in crops, including the high-salinity stress.

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