scholarly journals NATURAL NOCARDIOSIS IN CATTLE: A SURVEY AND PATHOLOGIGIAL STUDY

2000 ◽  
Vol 24 (2) ◽  
pp. 149-168
Author(s):  
M. J. ALWAN
Keyword(s):  
Microscopic Examination ◽  
Pure Culture ◽  
Nocardia Asteroides ◽  
Bacteriological Examination ◽  
Milk Samples ◽  
Granulomatous Lesions ◽  
Nodular Lesions ◽  
Gross Examination

This work was done to investigate Nocardiosis in cattle in order to evaluate the incidence of nocardiali mastitis in Al- dejiala station, bacteriological examination was conducted on 90 milk samples obtaited from mastitic cows Nocardia asteroides was found in 25 out of 90 (27. 77% mastitic cows. This M.O was recorered in pure culture in 5. cases and as mixed with other microorganisms in 20 cases . The gross examination of the affected udders showed multiple nodular lesions in firm fibrotic udders, some of them formed draining sinus which exuded pus to the exterior multifocal nodules of varying size were seen in the lungs Microscopic examination of the affected  udders revealed pyogranulomatous lesions in different areas of the glandular parenchyma multiple classical granulomatous lesions were also noticed in the lung tissues.

scholarly journals Recovery of members of the Pasteurella-Actinobacillus-group from guineapigs

1983 ◽  
Vol 17 (4) ◽  
pp. 285-289 ◽  
Author(s):  
R. Boot ◽  
J. Oosterom ◽  
H. C. Walvoort
Keyword(s):  
Mammary Gland ◽  
Pure Culture ◽  
Post Mortem ◽  
Bacteriological Examination

In the course of post-mortem bacteriological examination of conventional guineapigs, 88 isolates belonging to the Pasteurella-Actinobacillus-group were recovered from 69 of 279 animals (25%). Most isolates were recovered from pneumonic lung, enteritic jejunum and inflamed mammary gland. No relationship was found between biotype and source of isolation. About 50% of isolates were recovered in pure culture or as the predominant micro-organism. It is concluded that members of the Pasteurella-Actinobacillus-group must be considered potentially pathogenic for guineapigs.

COMPARISON OF PLATE LOOP AND AGAR PLATE METHODS FOR BACTERIOLOGICAL EXAMINATION OF MANUFACTURING GRADE RAW MILK1,2

1967 ◽  
Vol 30 (4) ◽  
pp. 112-115 ◽  
Author(s):  
Sita Ramayya Tatini ◽  
Roger Dabbah ◽  
J. C. Olson
Keyword(s):  
Agar Plate ◽  
Bacterial Count ◽  
Raw Milk ◽  
Substantial Improvement ◽  
Plate Count ◽  
Bacteriological Examination ◽  
Standard Plate Count ◽  
Milk Samples ◽  
Standard Plate ◽  
Bulk Tank

Summary Plate loop counts and standard plate counts on each of several manufacturing grade raw milk samples (handled in cans or in farm bulk tanks) have been compared. On the average, the plate loop count (PLC) was lower than the standard plate count (SPC) regardless of the type of handling of milk on the farm, can or bulk tank. Agreement between the SPC and PLC seemed to depend upon the bacterial-count levels present in milk. Statistical analyses indicated significant differences, at 1% level of probability, between the average bacterial count by SPC and PLC methods regardless of count level (≤100,000/ml or >100,000/ml) in case of can milk samples. On the other hand, in case of farm bulk tank milk samples, no significant differences, at the 1% level of probability, between the average bacterial count by SPC and PLC methods were obtained, when the counts were equal to or less than 100,000 per ml; when the counts exceeded 100,000 per ml, significant differences were present. Since the bacterial counts of manufacturing grade raw milk samples are likely to exceed 100,000/ml, the data presented in this investigation indicate that, until the bacteriological quality of manufacturing grade milk supplies undergoes substantial improvement, the PLC method does not appear to be a suitable substitute for the SPC method for routine bacteriological examination of such milk supplies.

Use of milk amyloid A in the diagnosis of subclinical mastitis in dairy ewes

2013 ◽  
Vol 80 (4) ◽  
pp. 496-502 ◽  
Author(s):  
Arianna Miglio ◽  
Livia Moscati ◽  
Gabriele Fruganti ◽  
Michela Pela ◽  
Eleonora Scoccia ◽  
...  
Keyword(s):  
Health Status ◽  
Subclinical Mastitis ◽  
Farm Income ◽  
Control Group ◽  
Bacteriological Examination ◽  
Udder Health ◽  
Elisa Kit ◽  
Amyloid A ◽  
Milk Samples ◽  
Dairy Ewes

Subclinical mastitis (SM) is one of the most important diseases affecting dairy ewes worldwide, with negative impact on the animal health, farm income and public health. Animals with SM often remain untreated because the disease may not be revealed. Increase in somatic cell count (SCC) and positive bacteriology for mastitis pathogens in milk samples are indicative of SM but the evidence of only one of these alterations must suggest an uncertain SM (UM). UM is defined when positive bacteriological examination (Latent-SM) or SCC>500 000 cells/ml (non-specific-SM) are detected in milk. Nevertheless, SCC and bacteriological examination are expensive, time consuming and are not yet in use at the farm level in dairy ewes. Recently, a sensitive acute phase protein, amyloid A, displaying multiple isoforms in plasma and different body fluids including mammary secretion (milk amyloid A-MAA), has been investigated as a marker of mastitis in cows and, in a few studies, in sheep. The aim of this trial was to compare the concentration of MAA of single udder-halves in ewes with healthy udder-halves (HU-control group) and naturally occurring subclinical mastitis, both confirmed (SM group) and uncertain (UM groups: Latent-SM and non-specific-SM), for monitoring udder health. The reliability of a specific ELISA kit for the measurement of MAA was also tested. During a 3-month trial period, 153 udder halves were assigned to the experimental groups based on their health status: 25 with SM, 40 with UM (11 with latent-SM and 29 with non-specific-SM) and 88 HU. SCC and bacteriological analysis were performed to establish the control and subclinical mastitis groups. MAA concentrations in milk samples were measured using a specific commercially milk ELISA kit. The data were submitted to statistical analysis. Significant (P<0·05) differences among the groups SM, non-specific-SM and HU were detected with the SM having the highest level and HU the lowest. MAA concentration is affected by the udder health status and is a useful indicator of subclinical mastitis and increased SCC in sheep.

scholarly journals Tuberculosis in Kafue lechwe (Kobus leche kafuensis) and in a bushbuck (Tragelaphus scriptus) on a game ranch in Central Province, Zambia : case report

1998 ◽  
Vol 69 (3) ◽  
Author(s):  
U. Zieger ◽  
G.S. Pandey ◽  
N.P.J. Kriek ◽  
A.E. Cauldwell
Keyword(s):  
Neck Region ◽  
Wild Ungulates ◽  
Bacterial Isolation ◽  
Acid Fast Bacilli ◽  
Granulomatous Lesions ◽  
Kafue Lechwe ◽  
Nodular Lesions ◽  
Free Ranging ◽  
First Time

Mycobacteriosis was diagnosed for the first time outside a national park in free-ranging wild animals on a game ranch in Zambia. A Kafue lechwe (Kobus leche kafuensis) was found dead with tuberculous lesions on a ranch near Lusaka. Acid-fast bacilli were found in the affected organs. Mycobacteria were isolated from these tissues. A bushbuck (Tragelaphus scriptus) was found dead on the same ranch with multiple superficial abscesses in the neck region, extensive granulomatous lesions in the lung, the bronchial and mediastinal lymph nodes and several nodular lesions in the spleen. Few acid-fast bacilli were found in the exudate from the abscesses and lesions in the affected organs. Histologically the lesions resembled those of tuberculosis, but mycobacteria could not be isolated. In addition, 1 Kafue lechwe among 37 wild ungulates of 13 species shot on the ranch showed typical tuberculous lesions in the lungs, but the diagnosis was not confirmed by bacterial isolation. The role of the Kafue lechwe as maintenance host for tuberculosis as well as in the possible spread of this disease to other wildlife are discussed.

Chick Rearing II. The bacterial syndrome arising from a diet which is conducive to six-day disease

1945 ◽  
Vol 35 (2) ◽  
pp. 98-100 ◽  
Author(s):  
T. Barton Mann
Keyword(s):  
Bacterial Infection ◽  
Pure Culture ◽  
Saline Solution ◽  
Nutrient Agar ◽  
Bacteriological Examination ◽  
Inexpensive Method ◽  
Routine Examination ◽  
Negative Results

In his observations on six-day disease, Taylor (1944) reported that the chicks which died on his preliminary experiments gave negative results on bacteriological examination.Many workers hold that for routine examination of poultry, a quick, convenient and inexpensive method of detecting certain specific bacterial infection is to inoculate slopes of nutrient agar with liver or other tissue, and incubate at 37° C. It is claimed that some organisms often grow in pure culture from diseased chicks, and that this method is especially suitable in the detection of S. pullorum or S. gallinarum infections. The resultant growth can be washed off in saline solution and tested by specific agglutinating sera or by testing on certain carbohydrates.

scholarly journals Accessory sple­en in a dog: Macroscopic and microscopic findings

2021 ◽  
Vol 24 (2) ◽  
pp. 297-302
Author(s):  
M. N. Nazem ◽  
S. M. Sajjadian ◽  
R. Kheirandish ◽  
M. A. Shamshirgaran
Keyword(s):  
Microscopic Examination ◽  
Splenic Artery ◽  
Splenic Tissue ◽  
Variable Size ◽  
Mixed Breed ◽  
Microscopic Findings ◽  
Gross Examination ◽  
Accessory Spleens

Accessory spleens are one or more areas of normal ectopic splenic tissue of variable size supplied by the branches of the splenic artery. A 7 year-old mixed breed male dog was fixed for use as a model in anatomy hall. During the abdominal dissection, the gross examination showed a 6.3×3.1×1 cm mass that was situated on the caudodorsal border of the spleen. Its appearance and firm consistency were similar to those of spleen. There was no connection between the spleen and this mass. It was supplied by accessory splenic artery. Microscopic examination of the mass revealed some structures similar to spleen.

Ultrastructure of a Paratesticular Rhabdomyosarcoma

1990 ◽  
Vol 48 (3) ◽  
pp. 246-247
Author(s):  
Shirley Siew ◽  
Stanley M. Katlein
Keyword(s):  
Malignant Tumor ◽  
Microscopic Examination ◽  
Striated Muscle ◽  
Definitive Diagnosis ◽  
Single Site ◽  
Clinical Impression ◽  
Cystic Change ◽  
Neoplastic Tissue ◽  
Scrotal Mass ◽  
Gross Examination

Rhabdomyosarcoma, a malignant tumor of striated muscle, was described first in 1849 by Rokitansky. Since that time, another 150 cases have been documented. In recent years, it has been recognized that the paratesticular location of rhabdomyosarcoma is an important single site, particularly in adolescents. As a combination of surgery and chemotherapy can affect a cure, a definitive diagnosis should be established as early as possible.We report the case of an 18 year old boy, who presented with a scrotal mass, which was removed surgically, with the clinical impression of a hydrocele. On gross examination, the mass measured 4cm. He showed the presence of cystic change,foci of hemorrhage and solid white neoplastic tissue. Sheets of large polygonal cells were noted on histologic examination and there was infiltration of the epididymis.Microscopic examination of Epon embedded material cut at micron and stained with to luidine blue confirmed the presence of a diffuse infiltrate of large polygonal cells. The cells were arranged in sheets, which were separated by irregular fibrous bands.

Ozena treatment

1927 ◽  
Vol 23 (9) ◽  
pp. 973-974
Author(s):  
M. Venetsianova-Grudkova
Keyword(s):  
Pure Culture ◽  
Bacteriological Examination ◽  
Nasal Secretions

The author cites 58 cases of treatment of Ozena with a mixed vaccine, which included Coccobacillus Abel'i and Bacillus scleroma. Preliminary bacteriological examination of the nasal secretions of these patients yielded a pure culture of the Abelʹya microbe in 13 cases and in 22 cases a mixture with other bacteria; in 11 cases a coccus similar to Peretzʹa coccobacillus was found and in 3 cases a true Peretzʹa coccobacillus.

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