scholarly journals Recovery of members of the Pasteurella-Actinobacillus-group from guineapigs

1983 ◽  
Vol 17 (4) ◽  
pp. 285-289 ◽  
Author(s):  
R. Boot ◽  
J. Oosterom ◽  
H. C. Walvoort

In the course of post-mortem bacteriological examination of conventional guineapigs, 88 isolates belonging to the Pasteurella-Actinobacillus-group were recovered from 69 of 279 animals (25%). Most isolates were recovered from pneumonic lung, enteritic jejunum and inflamed mammary gland. No relationship was found between biotype and source of isolation. About 50% of isolates were recovered in pure culture or as the predominant micro-organism. It is concluded that members of the Pasteurella-Actinobacillus-group must be considered potentially pathogenic for guineapigs.

2000 ◽  
Vol 24 (2) ◽  
pp. 149-168
Author(s):  
M. J. ALWAN

This work was done to investigate Nocardiosis in cattle in order to evaluate the incidence of nocardiali mastitis in Al- dejiala station, bacteriological examination was conducted on 90 milk samples obtaited from mastitic cows Nocardia asteroides was found in 25 out of 90 (27. 77% mastitic cows. This M.O was recorered in pure culture in 5. cases and as mixed with other microorganisms in 20 cases . The gross examination of the affected udders showed multiple nodular lesions in firm fibrotic udders, some of them formed draining sinus which exuded pus to the exterior multifocal nodules of varying size were seen in the lungs Microscopic examination of the affected  udders revealed pyogranulomatous lesions in different areas of the glandular parenchyma multiple classical granulomatous lesions were also noticed in the lung tissues.


2016 ◽  
Vol 1 (1) ◽  
pp. 20-22
Author(s):  
Коссе ◽  
Vera Kosse ◽  
Полозюк ◽  
Olga Polozyuk ◽  
Лапина ◽  
...  

The aim of the research is justification of causal relationship patterns of Salmonella and Eimeria, and their role in the pathology of the disease. The authors analyzed the incidence of salmonellosis nutria and eimeriosis depending on the way of the content. 82 the corpses of nutria was conducted post-mortem autopsy and bacteriological examination. For bacteriological examination were selected the heart, lungs, liver with gallbladder, spleen, kidney, brain, mesenteric lymph nodes, bone from fresh corpses. It is established that in conditions of the same economy under the cellular content of the disease were not recorded, and if outdoor, how and when the content of the deep litter, and on the concrete floor had equally widespread. In the study of pathologic material derived from carcasses of dead nutria, have the following distribution of serovars: S. dublin – 22.8%, S. typhimurium – 40.3%, S. choleraesuis – is 36.9%. For bacteriological tests crops held at IBA, with marked diffuse haze. On Endo agar grew transparent slightly bluish or pinkish colonies 2-4 mm in diameter, on the environment Ploskirev – turbid, more dense, Levin – transparent with slight purple tint on the Wilson-Blair – black with a metallic luster. The culture obtained were studied by optical microscopy, detecting single spaced, do not form spores and capsules with a length of 1-4 µm, with a diameter of 0.5-0.8 μm, sticks. For associative flow of Salmonella and Eimeria nutria characteristic of chronic catarrhal gastritis, acute catarrhal-gemorragicescom-necrotic enterocolitis, congestive redness of the serous membranes of the small and large intestine, mesentery, granular and adipose dystrophy of liver, granular dystrophy of kidneys, myocardium, gemorragicescom-necrotic splenic, acute serous lymphadenitis, congestive hyperemia and edema of the lungs, brain and other organs, hemorrhagic diathesis.


1998 ◽  
Vol 35 (4) ◽  
pp. 235-240 ◽  
Author(s):  
V. Pérez ◽  
J. M. Corpa ◽  
J. F. García Marín ◽  
rizJ. J. Adú ◽  
H. E. Jensen

Mammary aspergillosis was diagnosed in four flocks of dairy sheep, comprising a total of 1,750 ewes. These animals had been treated prophylactically by intramammary infusion with cloxacillin 5 months prior to lambing. Mammary aspergillosis with concomitant spread to the regional lymph nodes was present in these flocks in a percentage ranging from 2% to 36.4% of treated sheep. Pathologic, bacteriologic, and mycologic studies were performed in seven of the affected ewes. Some of them also had lung, kidney, and liver involvement. The pathologic reaction within lesions ranged from the acute to subacute type, dominated by necrosis and vasculitis with thrombosis, to the chronic granulomatous type, with macrophages and giant cells. The distribution of lesions and the presence of a remarkable vasculitis with fungal thrombi in the mammary gland suggested a hematogenous dissemination of the infection from this organ. Immunologic staining with monoclonal antibody MAb-WF-AF-1, which reacts specifically with Aspergillus hyphae, identified the causative agent in histologic sections of the different affected tissues. The etiologic diagnosis was further supported by the isolation of Aspergillus fumigatus in pure culture from affected tissues and from eight samples of mammary secretions.


1945 ◽  
Vol 35 (2) ◽  
pp. 98-100 ◽  
Author(s):  
T. Barton Mann

In his observations on six-day disease, Taylor (1944) reported that the chicks which died on his preliminary experiments gave negative results on bacteriological examination.Many workers hold that for routine examination of poultry, a quick, convenient and inexpensive method of detecting certain specific bacterial infection is to inoculate slopes of nutrient agar with liver or other tissue, and incubate at 37° C. It is claimed that some organisms often grow in pure culture from diseased chicks, and that this method is especially suitable in the detection of S. pullorum or S. gallinarum infections. The resultant growth can be washed off in saline solution and tested by specific agglutinating sera or by testing on certain carbohydrates.


1966 ◽  
Vol 3 (6) ◽  
pp. 616-632 ◽  
Author(s):  
R. S. Hirth ◽  
S. W. Nielsen ◽  
W. N. Plastridge

A Mycoplasma, which was a natural pathogen for the bovine mammary gland was added to bull semen prior to processing and freezing in liquid nitrogen. Twelve virgin heifers were inseminated with frozen semen containing live Mycoplasma, and 6 with semen containing heat-killed organisms. Mycoplasma were recovered from cervico-vaginal mucus for up to 8 months after insemination, and also in pure culture from lesions observed at necropsy. Ten of 12 heifers receiving semen containing live Mycoplasma required multiple inseminations, and 4 of the 12 failed to conceive after as many as 5 inseminations. All heifers that received killed organisms conceived. At necropsy, the 4 heifers that failed to conceive had varying degrees of chronic suppurative salpingitis, chronic endometritis, and ovarian adhesions. The Mycoplasma was considered to be the cause of the genital lesions and the resultant infertility.


1927 ◽  
Vol 23 (9) ◽  
pp. 973-974
Author(s):  
M. Venetsianova-Grudkova

The author cites 58 cases of treatment of Ozena with a mixed vaccine, which included Coccobacillus Abel'i and Bacillus scleroma. Preliminary bacteriological examination of the nasal secretions of these patients yielded a pure culture of the Abelʹya microbe in 13 cases and in 22 cases a mixture with other bacteria; in 11 cases a coccus similar to Peretzʹa coccobacillus was found and in 3 cases a true Peretzʹa coccobacillus.


1899 ◽  
Vol 4 (3-4) ◽  
pp. 425-450 ◽  
Author(s):  
Franklin Warren White

Our conclusions from the literature and our own experiments may be summarized as follows: I. Blood for bacteriological examination during life should be taken directly from the veins and in considerable quantity. II. Resorption of toxines is the most important feature in cases of sepsis; pyogenie bacteria invade the general circulation in a rather small proportion even of severe eases, and, as a rule, late in the course of the disease. III. A general infection by the pnenmococcus can be demonstrated occasionally in the late stages of acute lobar pneumonia. IV. The value of blood cultures as a means of diagnosis in obscure cases of sepsis is limited by the fact that invasion of the blood by the specific organism cannot be demonstrated during life in the majority of cases. Positive cultures are very valuable; negative cultures do not exclude local septic infections. V. The detection of specific bacteria in the blood of cases of sepsis and of pneumonia gives a very unfavorable prognosis in most cases. VI. General terminal infections with pyogenic cocci occasionally occur as an immediate cause of death in chronic disease. Local infections processes play this part more frequently. VII. As far as our experiments have shown, invasion of the blood by bacteria during the death agony, with subsequent distribution of the genus to the organs by the circulation, is a rather uncommon occurrence. VIII. Owing to the relative infrequency of agonal invasion, we believe that in the majority of cases where the autopsy is performed promptly after death, the bacteria which are found in the organs succeeded in reaching these organs previously to the death agony, and are associated with the course of the disease. IX. The presence of bacteria in the organs of late autopsies is due in many cases to post-mortem extension from one organ to another, and in some cases to the post-mortem growth of small numbers of genus which were distributed to the organs by means of the circulation.


2017 ◽  
Vol 73 (8) ◽  
pp. 510-512 ◽  
Author(s):  
Aleksandra Ledwoń ◽  
Izabella Dolka ◽  
Beata Dolka ◽  
Andrzej Życzyński ◽  
Wojciech Bielecki

Mycobacterium fortuitum infection and adenocarcinoma were associated with cloacal prolapse in a White’s tree frog (Litoria caerulea). The disease was characterized by the presence of nodules in a cloacal mucous membrane. Bacteriological examination of the cloacal tissue revealed the presence of Enterococcus sp., E. coli and numerous acid-fast bacteria (AFB). The presence of AFB was confirmed by a Ziehl-Neelsen stain of the cloacal tissue and culture on the Löwenstein–Jensen medium. Post-mortem examination showed no changes typical of mycobacteriosis or neoplasia in other organs.


Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1851
Author(s):  
Daria Urbańska ◽  
Ryszard Puchała ◽  
Justyna Jarczak ◽  
Michał Czopowicz ◽  
Jarosław Kaba ◽  
...  

The aim of this study was to determine whether asymptomatic small ruminant lentivirus seropositive (SRLV-SP) goats were more susceptible to bacterial infection of the udder when lactating by comparing the presence and species of pathogenic bacteria in their milk with the values for seronegative goats (SRLV-SN). Milk samples were collected during morning milking on days 20, 40, 60, 150, and 210 of lactation for three consecutive years and subjected to bacteriological examination. Staphylococcus caprae and S. xylosus were the most frequent strains identified in both SRLV-SP and SRLV-SN goats. The prevalence of pathogenic bacteria was the highest in the 1st lactation, regardless of SRLV status. Moreover, the prevalence of pathogenic bacteria was significantly higher in SRLV-SP goats, but only those in the 5th or further lactation (p = 0.010). This suggests a relationship between long-lasting SRLV infection and susceptibility to bacterial infections of the udder.


Author(s):  
C.M. Mulei ◽  
T.A. Ngatia

From a group of 11 recently weaned pigs, 4 were reported to be sick. Clinical examination of the sick pigs revealed marked dyspnoea, bluish-red discolouration of the skin, incoordination and difficulty in walking. Bacteriological examination of the gut contents of 2 pigs that had died earlier yielded pure cultures of haemolytic Escherichia coli. Post mortem examination of the remaining 2 pigs that died subsequently revealed progressive pulmonary collapse. One of these also showed subcutaneous oedema of the head and marked oedema of the mesentery of the spiral colon and oedema of the brain. Microscopically there was pulmonary alveolar collapse and degenerative changes in the liver.On the basis of the clinical signs, isolation of haemolytic E. coli and the post mortem findings, a diagnosis of oedema disease was made.


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