scholarly journals A preliminarıy work on the cold sensitivity of Casuarina cunninghamiana (Casuarinaceae) saplings

2021 ◽  
Author(s):  
Ian Timothy RILEY ◽  
Ayşe Hayriye SAYGI
Keyword(s):  
Cold Sensitivity ◽  
Casuarina Cunninghamiana

Cytokine analysis in patients with different stages of thromboangiitis obliterans

2020 ◽  
Vol 20 ◽  
Author(s):  
Abbas Shapouri-Moghaddam ◽  
Seyed Jalil Tavakkol Afshari ◽  
Mohammad-Hadi Saeed Modaghegh ◽  
Hamid Reza Rahimi ◽  
Mahmoud Mahmoudi ◽  
...  
Keyword(s):  
Transforming Growth Factor ◽  
Vascular Diseases ◽  
Transforming Growth Factor Β ◽  
Serum Levels ◽  
Interferon Γ ◽  
Analysis Of Covariance ◽  
Cold Sensitivity ◽  
Thromboangiitis Obliterans ◽  
Burning Pain ◽  
Cytokine Analysis

Background: Studies suggest that cytokines are involved in the development of both inflammatory disorders and vascular diseases. Objective: The changes in transforming growth factor β (TGFβ), interleukin 6 (IL6), tumor necrosis factor α (TNFα), and interferon γ (IFNγ) with the progression of the thromboangiitis obliterans (TAO) symptomswereinvestigated in this research. Methods: This study included 80 patients with TAO, who were selected from the Vascular and Endovascular Research Center in Alavi Hospital between 2012 and 2016. They were then categorized into three groups: mild (migratory thrombophlebitis, cold sensitivity or Raynaud's phenomenon, and skin discoloration), moderate (chronic ulcers, claudication, and burning pain of the feet at night), and severe (pain at rest and spontaneous gangrene) symptoms. The serum levels of TGFβ, IL6, TNFα, and IFNγwere determinedby the ELISA method and compared among the groups. Results: The first three predominant symptoms were pulse disorder (n = 76, 95.00%), cold intolerance (n = 61, 76.25%), and claudication (n = 59, 73.75%). A comparison of the analysis of covariance (ANCOVA) revealed that both TGFβ and IL6 were dysregulatedas the severity of the symptoms increased from the moderate to the severe stages; however, such changes were not significant(p > 0.05). In the multiple logistic regression model, increased TNFαlevelswere seen in the presence of the moderatesymptoms as compared to the severe ones (p < 0.05). Conclusion: It could be concluded that TNFα, as part of the defining cytokine-production profile of Thelper cells, can be significantly involved in the progression of TAO from the moderate to the severe stages.

Suppressors of a Cold-Sensitive Mutation in Yeast U4 RNA Define Five Domains in the Splicing Factor Prp8 That Influence Spliceosome Activation

Genetics ◽  
2000 ◽  
Vol 155 (4) ◽  
pp. 1667-1682 ◽  
Author(s):  
Andreas N Kuhn ◽  
David A Brow
Keyword(s):  
Large Scale ◽  
Splicing Factor ◽  
Cold Sensitivity ◽  
Splicing Factors ◽  
Loss Of Function ◽  
U1 Snrnp ◽  
Snrnp Protein ◽  
Cold Sensitive ◽  
Spliceosome Activation ◽  
U4 Rna

AbstractThe highly conserved splicing factor Prp8 has been implicated in multiple stages of the splicing reaction. However, assignment of a specific function to any part of the 280-kD U5 snRNP protein has been difficult, in part because Prp8 lacks recognizable functional or structural motifs. We have used a large-scale screen for Saccharomyces cerevisiae PRP8 alleles that suppress the cold sensitivity caused by U4-cs1, a mutant U4 RNA that blocks U4/U6 unwinding, to identify with high resolution five distinct regions of PRP8 involved in the control of spliceosome activation. Genetic interactions between two of these regions reveal a potential long-range intramolecular fold. Identification of a yeast two-hybrid interaction, together with previously reported results, implicates two other regions in direct and indirect contacts to the U1 snRNP. In contrast to the suppressor mutations in PRP8, loss-of-function mutations in the genes for two other splicing factors implicated in U4/U6 unwinding, Prp44 (Brr2/Rss1/Slt22/Snu246) and Prp24, show synthetic enhancement with U4-cs1. On the basis of these results we propose a model in which allosteric changes in Prp8 initiate spliceosome activation by (1) disrupting contacts between the U1 snRNP and the U4/U6-U5 tri-snRNP and (2) orchestrating the activities of Prp44 and Prp24.

scholarly journals Cold Sensitivity and Other Phenotypes Resulting from Mutation in PyrA Gene

1969 ◽  
Vol 244 (15) ◽  
pp. 4039-4045
Author(s):  
A Abd-El-Al ◽  
J L Ingraham
Keyword(s):  
Cold Sensitivity

scholarly journals The tubulin cofactor A is involved in hyphal growth, conidiation and cold sensitivity in Fusarium asiaticum

2015 ◽  
Vol 15 (1) ◽  
pp. 35 ◽  
Author(s):  
Xiaoping Zhang ◽  
Xiang Chen ◽  
Jinhua Jiang ◽  
Menghao Yu ◽  
Yanni Yin ◽  
...  
Keyword(s):  
Hyphal Growth ◽  
Cold Sensitivity ◽  
Fusarium Asiaticum

Sequential cold-sensitive mutations in Aspergillus fumigatus

1978 ◽  
Vol 24 (2) ◽  
pp. 84-88 ◽  
Author(s):  
Ole Nielsen ◽  
Kenneth F. Gregory
Keyword(s):  
Aspergillus Fumigatus ◽  
Type A ◽  
Maximum Growth ◽  
Cold Sensitivity ◽  
Nonpermissive Temperature ◽  
Wild Type ◽  
Cold Sensitive ◽  
Wild Type Parent ◽  
Thermotolerant Fungus

Mutants of the thermotolerant fungus Aspergillus fumigatus I-21 (ATCC 32722) unable to grow at 37 °C were sought. Cold-sensitive mutants were enriched from progeny spores of γ-irradiated conidia by two or more incubations at various nonpermissive temperatures alternating with filtrations through cheesecloth. The approximate minimum, optimum, and maximum growth temperatures of the parent were 12, 40, and 50 °C, respectively. Mutants unable to grow at 37 °C were not successfully isolated directly from the wild type. A mutant unable to grow at 25 °C was isolated and mutations further increasing the cold sensitivity by increments of 3–5 °C were found to occur. Mutants completely unable to grow at 37 °C were obtained by five sequential mutations. All mutants grew as fast as the wild-type parent at 45 °C and higher. Each mutant produced revenants able to grow not only at the nonpermissive temperature used for its isolation but also at lower temperatures.

Endothelial damage in perfused dog kidney and cold sensitivity of vascular NaK-ATPase

Cryobiology ◽  
1972 ◽  
Vol 9 (5) ◽  
pp. 457-460 ◽  
Author(s):  
Folkert O. Belzer ◽  
Robert Hoffman ◽  
Josephine Huang ◽  
Glenn Downes
Keyword(s):  
Endothelial Damage ◽  
Cold Sensitivity ◽  
Dog Kidney

scholarly journals Super Cooling Point Phenotypes and Cold Resistance in Hyles euphorbiae Hawk Moths from Different Climate Zones

Diversity ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 207
Author(s):  
Hana Daneck ◽  
Matthias Benjamin Barth ◽  
Martin Geck ◽  
Anna K. Hundsdoerfer
Keyword(s):  
Environmental Conditions ◽  
Cold Hardiness ◽  
Cold Treatment ◽  
Underlying Mechanism ◽  
Frost Tolerance ◽  
Cold Sensitivity ◽  
Cold Hardy ◽  
Hyles Euphorbiae

The spurge hawkmoth Hyles euphorbiae L. (Sphingidae) comprises a remarkable species complex with still not fully resolved taxonomy. Its extensive natural distribution range covers diverse climatic zones. This predestinates particular populations to cope with different local seasonally unfavorable environmental conditions. The ability of the pupae to overcome outer frosty conditions is well known. However, the differences between two main ecotypes (‘euphorbiae’ and ‘tithymali’) in terms of the inherent degree of frost tolerance, its corresponding survival strategy, and underlying mechanism have not been studied in detail so far. The main aim of our study was to test the phenotypic exhibition of pupae (as the relevant life cycle stadia to outlast unfavorable conditions) in response to combined effects of exogenous stimuli, such as daylight length and cooling regime. Namely, we tested the turnout of subitan (with fast development, unadapted to unfavorable conditions) or diapause (paused development, adapted to unfavorable external influences and increased resistance) pupae under different conditions, as well as their mortality, and we measured the super cooling point (SCP) of whole pupae (in vivo) and pupal hemolymph (in vitro) as phenotypic indicators of cold acclimation. Our results show higher cold sensitivity in ‘tithymali’ populations, exhibiting rather opportunistic and short-termed cold hardiness, while ‘euphorbiae’ produces a phenotype of seasonal cold-hardy diapause pupae under a combined effect of short daylight length and continuous cold treatment. Further differences include the variability in duration and mortality of diapause pupae. This suggests different pre-adaptations to seasonal environmental conditions in each ecotype and may indicate a state of incipient speciation within the H. euphorbiae complex.

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