Correlation of the Pathogenic Bacteria Isolated from Sputum Samples with Age, Sex, Seasonal Variation and Determination of Their Antibiotic Resistance Pattern

Background: Staphylococcus aureus (S. aureus) is a potential pathogen responsible for producing various infectious diseases. Determination of the prevalence of S. aureus infections both in animals and humans and elucidation of their antibiotic resistance pattern is crucial. The objective of this study was to find out the prevalence of Staphylococcal diseases in animals and humans, and their resistance pattern to commonly used antibiotics. Methods: A total of 100 animal and 100 human clinical samples were analyzed by traditional method. The S. aureus was identified by their cultural characteristics, gram’s staining, and catalase and coagulase tests. Antibiotic resistance pattern of the isolates was determined by disc diffusion method using various types of antibiotics. Results: The prevalence of S. aureus in animals and humans were 54% and 40%, respectively. S. aureus isolates of animal origin were highly resistant against penicillin (64.81%) and oxytetracycline (42.59%), and the lowest resistance was against oxacillin (7.40 %). S. aureus isolates from human were also showed a higher percentage of resistance against penicillin (87.5%) then oxacillin, cloxacillin, amoxicillin (37.5% each), and lowest resistance was observed against fusidic acid (5%). Conclusion: The findings of this study will certainly help veterinary clinicians or physicians to select appropriate antibiotics like oxacillin and fusidic acid for the treatment of different types of staphylococcal infections.

Download Full-text

Identification and Determination of Antibiotic Resistance Pattern of Stenotrophomonas maltophilia Isolated form Medical Devices and Clinical Samples in Jahrom,s Hospitals by Phenotype and Molecular Methods.

Pars of Jahrom University of Medical Sciences ◽

10.29252/jmj.14.2.43 ◽

2016 ◽

Vol 14 (2) ◽

pp. 43-50

Author(s):

Aboutaleb Nikpour ◽

Manoochehr Shabani ◽

Akbar Kazemi ◽

Maryam Mohandesi ◽

Roya Ershadpour ◽

...

Keyword(s):

Antibiotic Resistance ◽

Medical Devices ◽

Stenotrophomonas Maltophilia ◽

Molecular Methods ◽

Clinical Samples ◽

Resistance Pattern ◽

Antibiotic Resistance Pattern

Download Full-text

Antibiotic Resistance Pattern and Prevalence of blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX, tetA and tetB Genes in Acinetobacter baumannii Isolated from Clinical Specimens of Hospitals in Tabriz city, Iran

Journal of Clinical Research in Paramedical Sciences ◽

10.5812/jcrps.118521 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Abolfazl Jafari Sales ◽

Sara Naebi ◽

Hossein Bannazadeh-Baghi ◽

Morteza Saki

Keyword(s):

Antibiotic Resistance ◽

Acinetobacter Baumannii ◽

Pathogenic Bacteria ◽

Resistance Pattern ◽

Cross Sectional ◽

Beta Lactamases ◽

Clinical Specimens ◽

Antibiotic Resistance Pattern ◽

Extended Spectrum Beta Lactamases ◽

Tabriz City

Background: Nowadays, the resistance of pathogenic bacteria to antibiotics has become a global problem. Acinetobacter baumannii is an important opportunistic nosocomial pathogen. Acinetobacter baumannii plays a significant role in antibiotic resistance. Objectives: The purpose of this study was to investigate the prevalence of the blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes and antibiotic resistance pattern of A. baumannii isolated from hospitals in Tabriz city, Iran. Methods: This study was descriptive cross-sectional research, performed on 129 isolates of Acinetobacter from different clinical specimens. The Isolates were identified using standard laboratory methods and culture in selective mediums. The antibiotic resistance pattern of isolates was also determined by the Kirby-Bauer disk diffusion susceptibility test. Phenotypic and genotypic detection of blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes in the isolates was carried out by a combined disk test (CDT) and polymerase chain reaction (PCR), respectively. Results: The highest resistance of isolates was determined to cefotaxime (100%) and ceftazidime (100%). The results of CDT showed that 14 (12.96%) isolates could produce extended-spectrum Beta-lactamases (ESBLs). However, the PCR results blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes showed that these genes were in 100%, 18.51%, 16.66%, 32.40%, 16.66%, 31.48%, 32.40% and 21.29% of isolates, respectively. Conclusions: Due to the high prevalence of antimicrobial resistance in strains, rapid and timely detection of antibiotic-resistant A. baumannii strains is necessary for the selection of an appropriate therapeutic approach and prevention of their prevalence.

Download Full-text