scholarly journals Antibiotic Resistance Pattern and Prevalence of blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX, tetA and tetB Genes in Acinetobacter baumannii Isolated from Clinical Specimens of Hospitals in Tabriz city, Iran

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Abolfazl Jafari Sales ◽  
Sara Naebi ◽  
Hossein Bannazadeh-Baghi ◽  
Morteza Saki
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Pathogenic Bacteria ◽  
Resistance Pattern ◽  
Cross Sectional ◽  
Beta Lactamases ◽  
Clinical Specimens ◽  
Antibiotic Resistance Pattern ◽  
Extended Spectrum Beta Lactamases ◽  
Tabriz City

Background: Nowadays, the resistance of pathogenic bacteria to antibiotics has become a global problem. Acinetobacter baumannii is an important opportunistic nosocomial pathogen. Acinetobacter baumannii plays a significant role in antibiotic resistance. Objectives: The purpose of this study was to investigate the prevalence of the blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes and antibiotic resistance pattern of A. baumannii isolated from hospitals in Tabriz city, Iran. Methods: This study was descriptive cross-sectional research, performed on 129 isolates of Acinetobacter from different clinical specimens. The Isolates were identified using standard laboratory methods and culture in selective mediums. The antibiotic resistance pattern of isolates was also determined by the Kirby-Bauer disk diffusion susceptibility test. Phenotypic and genotypic detection of blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes in the isolates was carried out by a combined disk test (CDT) and polymerase chain reaction (PCR), respectively. Results: The highest resistance of isolates was determined to cefotaxime (100%) and ceftazidime (100%). The results of CDT showed that 14 (12.96%) isolates could produce extended-spectrum Beta-lactamases (ESBLs). However, the PCR results blaOXA-51, blaNDM, blaVIM, blaPER, blaVEB, blaCTX-M, tetA and tetB genes showed that these genes were in 100%, 18.51%, 16.66%, 32.40%, 16.66%, 31.48%, 32.40% and 21.29% of isolates, respectively. Conclusions: Due to the high prevalence of antimicrobial resistance in strains, rapid and timely detection of antibiotic-resistant A. baumannii strains is necessary for the selection of an appropriate therapeutic approach and prevention of their prevalence.

The Antibiotic Resistance Pattern and Prevalence of blaTEM, blaSHV, blaCTX-M, blaPSE-1, sipB/C, and cmlA/tetR Genes in Salmonella typhimurium Isolated from Children with Diarrhea in Tabriz, Iran

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Abolfazl Jafari Sales ◽  
Sara Naebi ◽  
Rozita Nasiri ◽  
Hossein Bannazadeh-Baghi
Keyword(s):  
Antibiotic Resistance ◽  
Salmonella Typhimurium ◽  
Resistance Genes ◽  
Health Concern ◽  
Resistance Pattern ◽  
Cross Sectional ◽  
Fecal Samples ◽  
Beta Lactamases ◽  
Extended Spectrum Beta Lactamases ◽  
Synergy Test

Background: Salmonella gastroenteritis is a global health concern. Recently, increased resistance to Salmonella typhimurium has been reported in several countries. Objectives: The present study aimed to evaluate the prevalence of blaTEM, blaSHV, blaCTX-M, blaPSE-1, sipB/C, and cmlA/tetR genes in S. typhimurium isolates and determine their antibiotic resistance. Methods: This cross-sectional descriptive study was conducted on 110 fecal samples, which were collected from the patients referred to the hospitals and medical centers in Tabriz, Iran during eight months. After phenotypic identification, the antibiogram test and double-disc synergy test were performed on the isolates. Following that, the prevalence of resistance genes was evaluated using multiplex PCR and specific primers. Results: Out of 110 fecal samples, 26 samples (23.63%) were positive for S. typhimurium. The highest resistance of the isolates was against ceftazidime, cefotaxime, amikacin, and tetracycline (100%), and the lowest resistance was against imipenem (3.85%) and nalidixic acid (7.69%). In total, 15 S. typhimurium isolates (57.69%) were positive for the extended-spectrum beta-lactamases. In addition, the most common resistance genes in the isolates were cmlA/tetR (38.46%), blaTEM (34.61%), and blaCTX-M (26.92%). Four isolates (15.38%) carried sipB, three isolates (11.53%) contained blaSHV, and two isolates (7.69%) carried blaPSE-1. Conclusions: The obtained results indicated the high prevalence of antibiotic-resistant S. typhimurium. Therefore, the identification of resistance genes is an important strategy for identifying and counteracting antibiotic resistance.

scholarly journals Antibiotic resistance pattern of Klebsiella pneumoniae isolates producing extended spectrum beta-lactamases in Zanjan city, Iran

2020 ◽  
pp. 1-6
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Resistance Pattern ◽  
Beta Lactamases ◽  
Antibiotic Resistance Pattern ◽  
Extended Spectrum ◽  
Resistance Patterns ◽  
Extended Spectrum Beta Lactamases ◽  
Antibiotic Resistance Patterns

Extended spectrum beta-lactamases (ESBL) in Klebsiella pneumoniae isolates have led to increased antibiotic resistance and mortality in patients. Therefore, the present study was performed to investigate the susceptibility and antibiotic resistance patterns of ESBL- producing K. pneumoniae strains isolated from patients referred to Zanjan hospitals.

Molecular Characterization and Antibiotic Resistance Pattern of Isolated Acinetobacter baumannii in Iran

Gene Reports ◽  
2021 ◽  
pp. 101195
Author(s):  
Hossein Masoumi-Asl ◽  
Fatemah Sadeghpour Heravi ◽  
Ali Badamchi ◽  
Khadijeh Khanaliha ◽  
Marziye Farsimadan ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Molecular Characterization ◽  
Resistance Pattern ◽  
Antibiotic Resistance Pattern

scholarly journals The Frequency of Antibiotic Resistance and ESBLs Among Clinically Acinetobacter baumannii Strains Isolated from Patients in a Major Hospital in Tehran, Iran

2018 ◽  
Vol 12 (1) ◽  
pp. 254-260 ◽  
Author(s):  
Reza Ranjbar ◽  
Sajjad S. Tolon ◽  
Shahin Zayeri ◽  
Mehrdad Sami
Keyword(s):  
Antibiotic Resistance ◽  
Acinetobacter Baumannii ◽  
Bacterial Resistance ◽  
Treatment Options ◽  
Clinical Samples ◽  
Limiting Factor ◽  
Beta Lactamases ◽  
Microbiological Methods ◽  
Extended Spectrum Beta Lactamases ◽  
Amoxicillin Clavulanic Acid

Background:Bacterial resistance to antibiotics limits treatment options, increases morbidity and mortality, and raises the risk of antibiotic-associated adverse events. Antibacterial resistance emerges rapidly following an increase in the consumption of antibiotics against infectious diseases. The spread of ESBL producing strains has a limiting factor based on antibiotic function for the treatment of infections particularly caused byAcinetobacter baumannii(A. baumannii).Objective:This study was conducted to evaluate the prevalence of antimicrobial resistance and distribution ofblaTEM,blaCTX, andblaSHVgenes amongA. baumanniistrains isolated from clinical samples at a major hospital in Teheran, Iran.Methods:A. baumanniistrains were isolated and identified using standard microbiological methods. The disc diffusion and combined discs methods were used for testing antimicrobial susceptibility and to identify the strains producing Extended-Spectrum Beta-Lactamases (ESBL), respectively. DNA extraction was done by boiling method. Finally, the frequency of resistant genes includingblaTEM,blaCTX, andblaSHVin ESBL producing isolates was studied by PCR.Results:Gender distribution in this study was 53 (53%) samples for men and 47 (47%) for women. Totally, one hundredA. baumanniistrains were isolated. More than 93% of the isolates were multi drug resistant. The highest to lowest antibiotic resistance was observed against amoxicillin/clavulanic acid (98%), ceftriaxone (96%), cefotaxime (94%), and ceftazidime (93%), respectively. The frequency of positive phenotypic test of ESBL was 19% and 16% for CAZ-C and CTX-C, respectively. The frequency ofblaTEM,blaCTX, andblaSHVgenes was 52.1, 43.4, and 21.7, respectively.Conclusion:A. baumanniiisolates exhibited an extremely worrying level of antibiotic resistance, and a high percentage of the isolates showed MDR in this study. This is a serious warning because ESBLs are a major threat to the effectiveness of antibiotics that are currently available for medical uses. The frequency of genes encoded ESBL isolates ofA. baumanniimay be due to overuse and misuse of antibiotics.

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