Novel drought-inducible Cys2/His2-type zinc finger protein STF-2 from soybean (Glycine max) enhances drought tolerance in transgenic plants

2019 ◽  
Vol 51 (3) ◽  
Author(s):  
Bing Song ◽  
Yu Zhang ◽  
Yu Li ◽  
Yongping Fu ◽  
Piwu Wang
Keyword(s):  
Glycine Max ◽  
Transgenic Plants ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

Zinc-finger protein MdBBX7/MdCOL9, a target of MdMIEL1 E3 ligase, confers drought tolerance in apple

2021 ◽  
Author(s):  
Pengxiang Chen ◽  
Fang Zhi ◽  
Xuewei Li ◽  
Wenyun Shen ◽  
Mingjia Yan ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
E3 Ligase ◽  
26S Proteasome ◽  
Negative Regulator ◽  
Binding Motif ◽  
Ethylene Response Factor ◽  
Finger Protein

Abstract Water deficit is one of the main challenges for apple (Malus × domestica) growth and productivity. Breeding drought-tolerant cultivars depends on a thorough understanding of the drought responses of apple trees. Here, we identified the zinc-finger protein B-BOX 7/CONSTANS-LIKE 9 (MdBBX7/MdCOL9), which plays a positive role in apple drought tolerance. The overexpression of MdBBX7 enhanced drought tolerance, whereas knocking down MdBBX7 expression reduced it. Chromatin immunoprecipitation-sequencing (ChIP-seq) analysis identified one cis-element of MdBBX7, CCTTG, as well as its known binding motif, the T/G box. ChIP-seq and RNA-seq identified 1,197 direct targets of MdBBX7, including ETHYLENE RESPONSE FACTOR (ERF1), EARLY RESPONSIVE TO DEHYDRATION 15 (ERD15), and GOLDEN2-LIKE 1 (GLK1) and these were further verified by ChIP-qPCR and electronic mobility shift assays. Yeast two-hybrid screen identified an interacting protein of MdBBX7, RING-type E3 ligase MYB30-INTERACTING E3 LIGASE 1 (MIEL1). Further examination revealed that MdMIEL1 could mediate the ubiquitination and degradation of MdBBX7 by the 26S proteasome pathway. Genetic interaction analysis suggested that MdMIEL1 acts as an upstream factor of MdBBX7. In addition, MdMIEL1 was a negative regulator of the apple drought stress response. Taken together, our results illustrate the molecular mechanisms by which the MdMIEL1–MdBBX7 module influences the response of apple to drought stress.

scholarly journals Overexpression of Two CCCH-type Zinc-Finger Protein Genes Leads to Pollen Abortion in Brassica campestris ssp. chinensis

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1287
Author(s):  
Liai Xu ◽  
Tingting Liu ◽  
Xingpeng Xiong ◽  
Weimiao Liu ◽  
Youjian Yu ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Zinc Finger ◽  
Pollen Development ◽  
Brassica Campestris ◽  
Zinc Finger Protein ◽  
Function Analysis ◽  
Pollen Grains ◽  
Functional Redundancy ◽  
Pollen Abortion ◽  
Finger Protein

The pollen grains produced by flowering plants are vital for sexual reproduction. Previous studies have shown that two CCCH-type zinc-finger protein genes in Brassica campestris, BcMF30a and BcMF30c, are involved in pollen development. Due to their possible functional redundancy, gain-of-function analysis is helpful to reveal their respective biological functions. Here, we found that the phenotypes of BcMF30a and BcMF30c overexpression transgenic plants driven by their native promoters were similar, suggesting their functional redundancy. The results showed that the vegetative growth was not affected in both transgenic plants, but male fertility was reduced. Further analysis found that the abortion of transgenic pollen was caused by the degradation of pollen contents from the late uninucleate microspore stage. Subcellular localization analysis demonstrated that BcMF30a and BcMF30c could localize in cytoplasmic foci. Combined with the studies of other CCCH-type genes, we speculated that the overexpression of these genes can induce the continuous assembly of abnormal cytoplasmic foci, thus resulting in defective plant growth and development, which, in this study, led to pollen abortion. Both the overexpression and knockout of BcMF30a and BcMF30c lead to abnormal pollen development, indicating that the appropriate expression levels of these two genes are critical for the maintenance of normal pollen development.

OsMSR15 encoding a rice C2H2-type zinc finger protein confers enhanced drought tolerance in transgenic Arabidopsis

2016 ◽  
Vol 59 (3) ◽  
pp. 271-281 ◽  
Author(s):  
Xin Zhang ◽  
Bin Zhang ◽  
Ming Juan Li ◽  
Xu Ming Yin ◽  
Li Fang Huang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Transgenic Arabidopsis ◽  
Finger Protein

A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants

2001 ◽  
Vol 25 (3) ◽  
pp. 247-259 ◽  
Author(s):  
Jong Cheol Kim ◽  
Sang Hyoung Lee ◽  
Yong Hwa Cheong ◽  
Cheol-Min Yoo ◽  
Soo In Lee ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Cold Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Finger Protein

scholarly journals The Barley Stripe Mosaic Virus expression system reveals the wheat C2H2 zinc finger protein TaZFP1B as a key regulator of drought tolerance

2019 ◽  
Author(s):  
Mario Houde ◽  
Arnaud Cheuk ◽  
François Ouellet
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Mosaic Virus ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Expression System ◽  
Functional Characterization ◽  
Barley Stripe Mosaic Virus ◽  
Finger Protein

Abstract Drought stress is one of the major factors limiting wheat production globally. Improving drought tolerance is important for agriculture sustainability. Although various morphological, physiological and biochemical responses associated with drought tolerance have been documented, the molecular mechanisms and regulatory genes that are needed to improve drought tolerance in crops require further investigation. We have used a novel 4-component version (for overexpression) and a 3-component version (for underexpression) of a barley stripe mosaic virus-based (BSMV) system for functional characterization of the C2H2-type zinc finger protein TaZFP1B in wheat. These expression systems avoid the need to produce transgenic plant lines and greatly speeds up functional gene characterization.Results We show that overexpression of TaZFP1B stimulates plant growth and up-regulates different oxidative stress-responsive genes under well-watered conditions. Plants that overexpress TaZFP1B are more drought tolerant at critical periods of the plant’s life cycle. Furthermore, RNA-Seq analysis revealed that plants overexpressing TaZFP1B reprogram their transcriptome, resulting in physiological and physical modifications that help wheat to grow and survive under drought stress. In contrast, plants transformed to underexpress TaZFP1B are significantly less tolerant to drought and growth is negatively affected.Conclusions This study clearly shows that the two versions of the BSMV system can be used for fast and efficient functional characterization of genes in crops. The extent of transcriptome reprogramming in plants that overexpress TaZFP1B indicates that the encoded transcription factor is a key regulator of drought tolerance in wheat.

Overexpression of the novel Zygophyllum xanthoxylum C2H2-type zinc finger gene ZxZF improves drought tolerance in transgenic Arabidopsis and poplar

Biologia ◽  
2016 ◽  
Vol 71 (7) ◽  
Author(s):  
Yanguang Chu ◽  
Weixi Zhang ◽  
Bin Wu ◽  
Qinjun Huang ◽  
Bingyu Zhang ◽  
...  
Keyword(s):  
Drought Tolerance ◽  
Zinc Finger ◽  
Zinc Finger Protein ◽  
Transgenic Arabidopsis ◽  
The Novel ◽  
Gene Encoding ◽  
Zinc Finger Gene ◽  
Drought Tolerant ◽  
Finger Protein ◽  
Zygophyllum Xanthoxylum

Abstract(Bunge) is a perennial woody succulent xerophyte that is one of the most drought-tolerant plant species identified to date. In this study, the gene encoding the novel C2H2-type zinc finger protein (ZFP)

Sign in / Sign up

Export Citation Format

Share Document