Sub Clinical Pregnancy Toxaemia Diagnostic Indicators and its Therapeutic Evaluation in Goats

Background: Periparturient mortality in goats have a great economic impact on the livelihood of marginal farmers. Pregnancy toxaemia, a metabolic disease in small ruminants occurs as a result of negative energy balance consequent to enhanced requirement for glucose by the developing fetuses in the last trimester (last 6 to 4 weeks) of gestation. The present study was aimed to identify diagnostic and prognostic indicators of pregnancy toxaemia. Methods: During the period October 2016 to September 2018, a total of 516 adult non descriptive does were brought to Veterinary University Peripheral Hospital, Madhavaram Milk Colony, Chennai - 51, of which 264 (51.16%) were treated for medical conditions. Among the does treated for various medical conditions, 72 does were in their last six weeks of gestation carrying twins/triplets and presented with the history of off feed. They were subjected to determination of blood β-hydroxybutyric acid (BHBA) level by means of a portable blood ketone and glucose monitoring system and qualitative urinalysis using urine dip stick. Does with BHBA level greater than 0.8 mmol/L and less than 1.6 mmol/L were classified as sub-clinical pregnancy toxaemic group (n=12) and BHBA level greater than 1.6 mmol/L were classified as clinical pregnancy toxaemic group (n=12) and subjected to therapy while the remaining 48 does had BHBA levels less than 0.8 mmol/L. The control animals were selected from adult Tellicherry does in the age group of 2 to 4 years maintained at Livestock Farm Complex (LFC), Madhavaram Milk Colony, Chennai - 600 051. Result: All the twelve does of sub-clinical pregnancy toxaemic group recovered completely with a cure rate of 100%, while in the clinical pregnancy toxaemic group the cure rate was only 33%. Reliable diagnostic indicators of pregnancy toxaemia include blood â-hyroxybutyric acid concentration (³ 0.8 mmol/L) and presence of ketone body, glucose and protein in urine, while hyperglycaemia in advanced pregnancy toxaemic does indicate fetal death.

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Experience of using time-lapse microscopy in IVF and ICSI programs

Medical alphabet ◽

10.33667/2078-5631-2020-16-47-50 ◽

2020 ◽

pp. 47-50

Author(s):

N. V. Saraeva ◽

N. V. Spiridonova ◽

M. T. Tugushev ◽

O. V. Shurygina ◽

A. I. Sinitsyna

Keyword(s):

Pregnancy Rate ◽

Embryo Transfer ◽

Selection Procedure ◽

Time Lapse ◽

Single Embryo Transfer ◽

Main Group ◽

Clinical Pregnancy ◽

Control Group ◽

Time Lapse Microscopy

In order to increase the pregnancy rate in the assisted reproductive technology, the selection of one embryo with the highest implantation potential it is very important. Time-lapse microscopy (TLM) is a tool for selecting quality embryos for transfer. This study aimed to assess the benefits of single-embryo transfer of autologous oocytes performed on day 5 of embryo incubation in a TLM-equipped system in IVF and ICSI programs. Single-embryo transfer following incubation in a TLM-equipped incubator was performed in 282 patients, who formed the main group; the control group consisted of 461 patients undergoing single-embryo transfer following a traditional culture and embryo selection procedure. We assessed the quality of transferred embryos, the rates of clinical pregnancy and delivery. The groups did not differ in the ratio of IVF and ICSI cycles, average age, and infertility factor. The proportion of excellent quality embryos for transfer was 77.0% in the main group and 65.1% in the control group (p = 0.001). In the subgroup with receiving eight and less oocytes we noted the tendency of receiving more quality embryos in the main group (р = 0.052). In the subgroup of nine and more oocytes the quality of the transferred embryos did not differ between two groups. The clinical pregnancy rate was 60.2% in the main group and 52.9% in the control group (p = 0.057). The delivery rate was 45.0% in the main group and 39.9% in the control group (p > 0.050).

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