scholarly journals The mia mutants of Streptomyces albus J1074 are prone to translational errors and susceptible to certain stressors

Author(s):  
O. Rydkin ◽  
◽  
O. Koshla ◽  
B. Ostas ◽  
◽  
...  

Streptomyces albus J1074 has been established by us as a convenient model to study different aspects of tRNALeuUAA-dependent regulatory mechanisms, that take place in genus Streptomyces. These mechanisms are important for proper morphological and physiological transitions of streptomycete colonies, such as the onset of antibiotic production in stationary phase of growth. The genes for post-transcriptional modification of adenosine residue in 37th position of tRNAXXA family (so called mia genes) were shown to be important for the aforementioned processes, most likely because they impact tRNALeuUAA among other tRNAs. Our results were largely consistent with what is known about mia mutations in the other model systems, such as yeast and enterobacteria. Nevertheless, we also revealed several differences from the model systems, such as decreased susceptibility to hydrogen peroxide. This prompted us to look deeper into the behavior of the mia mutants, particularly their response to different stress factors. Here we report that S. albus mia mutants exhibit increased mistranslation rate as compared to their parental strain. These mutants are more susceptible than the parental strain to disulfide stress inducer diamide and DNA repair stressor caffeine. In summary, although the deficiency in certain tRNA modification appears to cause identical or very similar response (such as elevated mistranslation) across all so far studied bacterial systems, it also induces species- or genus-specific effects (such as disparate effects on H2O2 susceptibility). These differences could be attributed to the peculiarities of organization/function of regulatory pathway governing the response to a given stress. The observed results are further discussed in the wider context of the role of tRNA modification pathway in bacterial biology.

2018 ◽  
Vol 22 ◽  
pp. 203-209
Author(s):  
B. O. Ostash ◽  
O. S. Yushchuk ◽  
O. T. Koshla ◽  
Y. Rebets ◽  
I. S. Ostash ◽  
...  

Aim. Moenomycins are phosphoglycolipid antibiotics produced almost exclusively by representatives of genus Streptomyces. These antibiotics directly inhibit peptidoglycan glycosyltransferases and are extremely active against cocci. Here we studied how antibiotic-producing actinobacteria protect themselves from toxic action of moenomycins. Methods. Microbiological and molecular genetic approaches were combined to reveal intrinsic levels and distribution of moenomycin resistance across actinobacteria genera, and to pinpoint genes contributing to moenomycin resistance in model strain Streptomyces coelicolor M145. Results. Out of 51 actinobacterial species (90 % of which Streptomyces) being tested, only Streptomyces albus J1074 turned out to be highly susceptible to moenomycin A, although resistant variants can be facilely raised. Several classes of mutations increased level of susceptibility of S. coelicolor to moenomycin, although in no case the latter was equal to what we observed in J1074 strain. Conclusions. Moenomycin resistance is widespread across actinobacteria, and it most likely is caused by a combination factors, such as richly decorated cell wall and organization of divisome apparatus. It is possible that moenomycin resistance mechanisms operating in actinobacteria and pathogenic cocci are different. Keywords: moenomycin, antibiotic resistance, peptidoglycan.


2020 ◽  
Vol 63 (9) ◽  
pp. 1-4
Author(s):  
Jun Tian ◽  
Leixin Ye ◽  
Yuling Yang ◽  
Yalin Zhang ◽  
Changhua Hu ◽  
...  

2019 ◽  
Vol 53 (1) ◽  
pp. 1-7 ◽  
Author(s):  
O. T. Koshla ◽  
I. V. Rokytskyy ◽  
I. S. Ostash ◽  
T. Busche ◽  
J. Kalinowski ◽  
...  

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Yousra Ahmed ◽  
Yuriy Rebets ◽  
Bogdan Tokovenko ◽  
Elke Brötz ◽  
Andriy Luzhetskyy

2017 ◽  
Vol 11 (2) ◽  
pp. 62-69
Author(s):  
Esraa Gh. Al-Sammak

يهدف البحث الى تسليط الضوء على دور التصنيف المتعدد Polyphasic والذي يضم كل من التصنيف المظهري والجيني لتحديد وتثبيت صفات النوع ضمن جنس الـStreptomyces   الصعب التصنيف نتيجة كثرة الانواع والتغاير الكبير في الصفات المظهرية اضافة لدورها في مجالات عدة منها البيئة من خلال تحملها العديد من املاح العناصرالثقيلة الموجودة في البيئات الملوثة بالهيدروكاربونات واشتراكها في التخلص من الملوثات. تم عزل وتشخيص ست  عزلات من البكتريا الخيطية  التابعة لجنس Streptomyces من عشرين عينة تربة ملوثة بالهيدروكاربونات وترب حدائق .  شخصت الى ثلاثة انواع اعتمادا على دراسة الصفات المظهرية والجينية من خلال دراسة تتابع جزء الـ16SrDNA باستخدام البادئ 27f  و 1392r  وشخصت سلالتين على انها تابعة للنوع     Streptomyces flavogriseus ATCC 33331 وسلالتين للنوع Streptomyces coelicolor A3(2) واخيرا سلالتين تابعة للنوع  Streptomyces albus J1074  . اعتمادا على الصفات المظهرية والبالغة 48 صفة  و باستخدام التصنيف العددي والربط باستخدام المعدل الموزون و معامل التشابه البسيط Simple matching coefficient (Ssm)  باستخدام البرنامج الاحصائي SPSS  , تعنقدت السلالات في ثلاثة عناقيد ضمن المخطط الشجري ضم  العنقود A سلالتين للنوعStreptomyces coelicolor   مرتبطة عند نسبة تشابه 99%  في حين تعنقدت سلالتي النوع Streptomyces albus  ضمن العنقود B  وعند نسبة تشابه 95% كما تعنقدت سلالتي النوع Streptomyces flavogriseus  ضمن العنقود C  وبنسبة تشابه 95% . اظهرت الانواع المعزولة مستعمرات طباشيرية رصاصية الى بيضاء وتميز الغزل الهوائي للنوع   Streptomyces albus بكونه حلزوني مكبوس متفرع وكثيف في حين تميز بكون خيوط الغزل الهوائي متعرج وغير متفرع للنوع  Streptomyces flavogriseus وظهر الغزل الهوائي للنوع  Streptomyces coelicolor متفرع متحلزن ومتعرج و جميعها منتجة لرائحة  التربة. اظهرت جميع الانواع قيد الدراسة حساسية  لاملاح كل من كلوريد وكبريتات الزئبق , في حين اظهرت جميع الانواع مقاومة لكل من كلوريد ونترات الكوبلت و كبريتات الزنك و نترات  وخلات الرصاص و كبريتات النيكل و خلات الفضة وثنائي اوكسيد التيتانيوم . اعطت جميع الانواع حساسية للمضاد الحيوي,Ciprofloxacin  10 مايكروغرام  , 10 Tobramycinمايكروغرام  ,  Gentamicin  10 مايكروغرام , Vancomycin 30 مايكروغرام , Amikacin10مايكروغرام, Imipenem 10مايكروغرام. كما  تعنقدت الانواع ايضا عند استخدام التصنيف الجيني الى ثلاثة عناقيد  ضمن الشجرة  التطورية وبنسب تشابه تراوحت بين  96.7- 99.4  %باستخدام  Clustal W  وطريقة maximum likelihood method  باستخدام برنامج Mega 5.


2013 ◽  
Vol 79 (20) ◽  
pp. 6447-6451 ◽  
Author(s):  
Jung-Hoon Lee ◽  
Marcha L. Gatewood ◽  
George H. Jones

ABSTRACTUsing insertional mutagenesis, we have disrupted the RNase III gene,rnc, of the actinomycin-producing streptomycete,Streptomyces antibioticus. Disruption was verified by Southern blotting. The resulting strain grows more vigorously than its parent on actinomycin production medium but produces significantly lower levels of actinomycin. Complementation of therncdisruption with the wild-typerncgene fromS. antibioticusrestored actinomycin production to nearly wild-type levels. Western blotting experiments demonstrated that the disruptant did not produce full-length or truncated forms of RNase III. Thus, as is the case inStreptomyces coelicolor, RNase III is required for antibiotic production inS. antibioticus. No differences in the chemical half-lives of bulk mRNA were observed in a comparison of theS. antibioticus rncmutant and its parental strain.


BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 97 ◽  
Author(s):  
Nestor Zaburannyi ◽  
Mariia Rabyk ◽  
Bohdan Ostash ◽  
Victor Fedorenko ◽  
Andriy Luzhetskyy

2017 ◽  
Vol 199 (8) ◽  
pp. 1175-1183 ◽  
Author(s):  
Oksana Koshla ◽  
Maria Lopatniuk ◽  
Ihor Rokytskyy ◽  
Oleksandr Yushchuk ◽  
Yuriy Dacyuk ◽  
...  

Molecules ◽  
2017 ◽  
Vol 22 (10) ◽  
pp. 1758 ◽  
Author(s):  
Jorge Fernández-De la Hoz ◽  
Carmen Méndez ◽  
José A. Salas ◽  
Carlos Olano

1982 ◽  
Vol 28 (6) ◽  
pp. 705-709 ◽  
Author(s):  
D. A. Zuberer ◽  
Mark Roth

Eighty-five isolates of actinomycetes were obtained from the rhizospheres of 10 indigenous grasses of central Texas. Of these, 10 were classified as members of the genus Nocardia; the remainder were members of the genus Streptomyces. To determine whether antibiotic production might be a significant factor in the establishment of associative symbioses between grasses and diazotrophic bacteria, 12 strains of free-living nitrogen-fixing bactria were cross streaked against each actinomycete isolate. This in vitro assay indicated that 78% of the 85 isolates were antagonistic to at least one of the 12 nitrogen-fixing bacterial strains.


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