ISSR Based Study of Fine Fescue (Festuca ovina L.) Highlighted the Genetic Diversity of Iranian Accessions

2020 ◽  
Vol 54 (3) ◽  
pp. 257-263
Author(s):  
Reza Mohammadi ◽  
Bahman Panahi ◽  
Saleh Amiri
Keyword(s):  
Genetic Diversity ◽  
Festuca Ovina ◽  
Fine Fescue

scholarly journals DNA Content and Ploidy Estimation of Festuca ovina Accessions by Flow Cytometry

2020 ◽  
Author(s):  
Yinjie Qiu ◽  
Sierra Hamernick ◽  
Joan Barreto Ortiz ◽  
Eric Watkins
Keyword(s):  
Flow Cytometry ◽  
Seed Size ◽  
Dna Content ◽  
Breeding Programs ◽  
Festuca Ovina ◽  
Ploidy Levels ◽  
Plant Germplasm ◽  
Wide Range ◽  
National Plant Germplasm System ◽  
Fine Fescue

ABSTRACTFestuca ovina is a fine fescue that is used as a low-input turfgrass. The ploidy levels of F. ovina accessions held by the USDA National Plant Germplasm System (NPGS) are unknown, limiting the use of the germplasm in breeding programs. The objective of this study was to determine DNA content and estimate ploidy of these 127 accessions. Among the accessions, we identified a wide range of ploidy levels from diploid to octoploid. We also found the accessions with higher ploidy levels usually had larger seed size. These results will be informative to plant breeders and researchers using germplasm from the F. ovina collection and point to challenges in maintaining polyploid, outcrossing germplasm seed stocks in common nurseries.

scholarly journals Genetic Structure and Eco-Geographical Differentiation of Wild Sheep Fescue (Festuca ovina L.) in Xinjiang, Northwest China

2017 ◽  
Author(s):  
Chenglin Zhang ◽  
Jianbo Zhang ◽  
Yan Fan ◽  
Ming Sun ◽  
Wendan Wu ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Gene Flow ◽  
Abiotic Factors ◽  
Northwest China ◽  
Group Method ◽  
Principal Coordinates Analysis ◽  
High Genetic Diversity ◽  
Festuca Ovina ◽  
Principal Coordinates ◽  
Genetic Diversity And Structure

Glaciation and mountain orogeny have generated new ecologic opportunities for plants, favoring an increase in the speciation rate. Moreover, they also act as corridors or barriers for plant lineages and populations. High genetic diversity ensures that species are able to survive and adapt. Gene flow is one of the most important determinants of the genetic diversity and structure of out-crossed species, and it is easily affected by biotic and abiotic factors. The aim of this study was to characterize the genetic diversity and structure of an alpine species, Festuca ovina L., in Xingjiang, China. A total of 100 individuals from 10 populations were analyzed using six amplified fragment length polymorphism (AFLP) primer pairs. A total of 583 clear bands were generated, of which 392 were polymorphic; thus, the percentage of polymorphic bands (PPB) was 67.24%. The total and average genetic diversities were 0.2722 and 0.2006 (0.1686-0.2225), respectively. The unweighted group method with arithmetic mean (UPGMA) tree, principal coordinates analysis (PCoA) and STRUCTURE analyses revealed that these populations or individuals could be clustered into two groups. The analysis of molecular variance analysis (AMOVA) suggested that most of the genetic variance existed within a population, and the genetic differentiation (Fst) among populations was 20.71%. The Shannon differentiation coefficient (G’st) among populations was 0.2350. Limited gene flow (Nm = 0.9571) was detected across all sampling sites. The Fst and Nm presented at different levels under the genetic barriers due to fragmentation. The population genetic diversity was significant relative to environmental factors such as temperature, altitude and precipitation.

Genetic diversity of Peucedanum japonicum using Internal Transcribed Spacer (ITS) sequence

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
YH Kim ◽  
JA Ryuk ◽  
BS Ko ◽  
JW Lee ◽  
SE Oh ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Internal Transcribed Spacer ◽  
Its Sequence

Genetic diversity revealed by ISSR molecular marker in different species of Ocimum

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
K Shinde ◽  
V Shinde ◽  
J Kurane ◽  
A Harsulkar ◽  
K Mahadik
Keyword(s):  
Genetic Diversity ◽  
Molecular Marker

Genetic diversity and phytochemical characterisation of Sideritis scardica populations from Greece

2019 ◽  
Author(s):  
EV Avramidou ◽  
E Sarrou ◽  
P Papaporfiriou ◽  
E Abraham
Keyword(s):  
Genetic Diversity

GENETIC DIVERSITY IN BAMBARA GROUNDNUT (Vigna subterranean) AS REVEALED BY MOLECULAR WEIGHTS OF THE SEEDS’ PROTEINS

2018 ◽  
Vol 30 (2) ◽  
pp. 19-28
Author(s):  
A. J. Oludare ◽  
J. I. Kioko ◽  
A. A. Akeem ◽  
A. T. Olumide ◽  
K. R. Justina ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Seed Proteins ◽  
Electrophoretic Analysis ◽  
Protein Characterization ◽  
Bambara Groundnut ◽  
Vigna Subterranea ◽  
Phylogenetic Relatedness ◽  
Molecular Weights ◽  
National Centre ◽  
Standard Marker

Nine accessions of Bambara groundnut (Vigna subterranea (L.) Verdc.,syn. Voandzeia subterranea (L.) Thouars ex DC.)  obtained from National Centre for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo state, were assessed for their genetic and phylogenetic relatedness through electrophoretic analysis of the seed proteins. 0.2g of the seeds were weighed and macerated with mortar and pestle in 0.2M phosphate buffer containing 0.133M of acid (NaH2PO4) and 0.067 of base (Na2HPO4) at pH 6.5. Protein characterization with standard marker revealed that the seeds of the nine accessions contained proteins (B.S.A, Oval Albumin, Pepsinogen, Trypsinogen and Lysozyme) with molecular weights ranging from 66kda and above, 45 – 65 kDa, 44 – 33 kda, 32-24 kDa and 23-14 kDa, respectively. The student T-test revealed that accessions B, C, E, F, H and I have molecular weights not significantly different from one another (P<0.05) while samples A, D and G showed significantly different values (P>0.05). All the accessions had at least two proteins and two major bands in common. The study revealed intra-specific similarities and genetic diversity in protein contents among the nine accessions of Bambara groundnut (Vigna subterraranea (L.) Verdc.syn

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