Donors of spring common wheat resistance to leaf rust and powdery mildew with genetic material of the species Aegilops speltoides L., Aegilops triuncialis L., and Triticum kiharae Dorof. et Migusch

Synthetic recombination form RS7 (BBAAUS), in which the first two genomes, A and B, originate from common wheat, and the third recombinant genome consists of Aegilops speltoides (S) and Ae. umbellulata (U) chromosomes, was obtained from crossing synthetic forms Avrodes (BBAASS) and Avrolata (BBAAUU). Resistant to leaf rust, yellow rust and powdery mildew, introgression lines have been obtained from backcrosses with the susceptible varieties of common wheat Krasnodarskaya 99, Fisht and Rostislav. PCR analysis showed the presence of amplification fragments with marker SCS421 specific for the Lr28 gene in the line 4991n17. The cytological study (С-banding and FISH) of 14 lines has revealed chromosomal modifications in 12 of them. In most cases, the lines carry translocations from Ae. speltoides, which were identified in chromosomes 1D, 2D, 3D, 2B, 4B, 5B and 7B. Also, lines with the substituted chromosomes 1S (1B), 4D (4S), 5D (5S) and 7D (7S) were identified. Lines that have genetic material from Ae. speltoides and Ae umbellulata at once were revealed. In the line 3379n14, translocations in the short arm of chromosome 7D from Ae. umbellulata and chromosomes 5BL, 1DL, 2DL from Ae. speltoides were revealed. The line 4626p16 presumably has a translocation on the long arm of chromosome 2D from Ae. umbellulata and the T7SS.7SL-7DL translocation from Ae. speltoides. The T1DS.1DL-1SL and T3DS.3DL-3SL translocations from Ae. speltoides, and T2DS.2DL-2UL and T7DL.7DS-7US from Ae. umbellulata have been obtained for the first time. These lines may carry previously unidentified disease resistance genes and, in particular, leaf rust resistance genes from Ae. speltoides and Ae. umbellulata.

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A tendency towards leaf rust resistance decrease in common wheat introgression lines with genetic material from Aegilops speltoides Tausch

Vavilov Journal of Genetics and Breeding ◽

10.18699/vj18.395 ◽

2018 ◽

Vol 22 (5) ◽

pp. 560-567

Author(s):

L. Ya. Plotnikova ◽

L. V. Meshkova ◽

E. I. Gultyaeva ◽

O. Р. Mitrofanova ◽

I. F. Lapochkina

Keyword(s):

Leaf Rust ◽

Common Wheat ◽

Rust Resistance ◽

Genetic Material ◽

Leaf Rust Resistance ◽

Introgression Lines ◽

Aegilops Speltoides ◽

Resistance Decrease

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Study of common wheat lines with genetic material Aegilops speltoides for leaf rust resistance

Abstract book of the 2nd International Scientific Conference "Plants and Microbes: the Future of Biotechnology" PLAMIC2020 ◽

10.28983/plamic2020.057 ◽

2020 ◽

Author(s):

E. R. Davoyan ◽

R. O. Davoyan ◽

Y. S. Zubanova ◽

D. S. Mikov ◽

D. M. Boldakov

Keyword(s):

Molecular Markers ◽

Leaf Rust ◽

Common Wheat ◽

Resistance Genes ◽

Rust Resistance ◽

Genetic Material ◽

Leaf Rust Resistance ◽

Aegilops Speltoides ◽

Wheat Lines ◽

Introgressive Lines

The results of evaluating introgressive lines by resistance to leaf rust and the presence of molecular markers in them linked to the known resistance genes Lr28, Lr35, Lr51, Lr10, Lr26, Lr34 are presented.

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Using the synthetic form RS5 to obtain new introgressive lines of common wheat

Vavilov Journal of Genetics and Breeding ◽

10.18699/vj21.088 ◽

2021 ◽

Vol 25 (7) ◽

pp. 770-777

Author(s):

R. O. Davoyan ◽

I. V. Bebyakina ◽

E. R. Davoyan ◽

Y. S. Zubanova ◽

D. M. Boldakov ◽

...

Keyword(s):

Leaf Rust ◽

Common Wheat ◽

Yellow Rust ◽

Aegilops Tauschii ◽

Genetic Material ◽

Leaf Rust Resistance ◽

Wild Relatives ◽

Aegilops Speltoides ◽

D Genome ◽

Synthetic Form

The use of the gene pool of wild relatives, which have a significant reserve of genetic diversity, is of immediate interest for breeding common wheat. The creation and use of synthetic forms as “bridges” is an effective method of transferring valuable genetic material from wild relatives to cultivated wheat. For this purpose, genome addition, genome substitution and recombinant “secondary” synthetic forms have been created in the P.P. Lukyanenko National Center of Grain. The synthetic recombination form RS5 (BBAASDt ), in which the third genome consists of chromosomes of Aegilops speltoides (S) and Aegilops tauschii (Dt ), was obtained from crossing the synthetic forms Avrodes (BBAASS) and M.it./Ae. tauschii (BBAADt Dt ), in which the D genome from Ae. tauschii was added to the BBAA genomes of the durum wheat cultivar Mutico italicum. Introgression lines resistant to leaf rust, yellow rust and powdery mildew have been obtained from backcrosses with the susceptible common wheat cultivars Krasnodarskaya 99, Rostislav and Zhirovka. Twelve resistant lines that additionally have high technological characteristics of grain and flour have been selected. The cytological study (С-banding) has revealed chromosomal modifications in 6 of 8 lines under study. The rearrangements mainly affected the chromosomes of the D genome, 1D, 3D, 4D, 6D and 7D. It was found that in most cases the genetic material from the synthetic form RS5 in the studied lines was represented by substituted chromosomes from Ae. tauschii. In line 5791p17, the substitution of chromosomes 6D from Ae. tauschii and 7D from Ae. speltoides was revealed. Substitutions 4D(4Dt ), 6D(6Dt ) from Ae. tauschii and 7D(7S) from Ae. speltoides were obtained for the first time. Molecular analysis of 12 lines did not reveal effective leaf rust resistance genes, presumably present in synthetic forms of M.it./Ae. tauschii and Avrodes. It is assumed that the lines may carry previously unidentified genes for fungal disease resistance, in particular for resistance to leaf rust, from Ae. tauschii and Ae. speltoides.

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Construction and study of leaf rust-resistant common wheat lines with translocations of Aegilops speltoides Tausch. Genetic material

Russian Journal of Genetics ◽

10.1134/s1022795412020020 ◽

2012 ◽

Vol 48 (4) ◽

pp. 404-409 ◽

Cited By ~ 11

Author(s):

I. G. Adonina ◽

N. V. Petrash ◽

E. M. Timonova ◽

Yu. A. Khristov ◽

E. A. Salina

Keyword(s):

Leaf Rust ◽

Common Wheat ◽

Genetic Material ◽

Aegilops Speltoides ◽

Wheat Lines

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Effectiveness of major resistance genes and identification of new sources for disease resistance in wheat

Acta Agronomica Hungarica ◽

10.1556/aagr.59.2011.3.7 ◽

2011 ◽

Vol 59 (3) ◽

pp. 241-248 ◽

Cited By ~ 2

Author(s):

G. Vida ◽

M. Cséplő ◽

G. Gulyás ◽

I. Karsai ◽

T. Kiss ◽

...

Keyword(s):

Disease Resistance ◽

Powdery Mildew ◽

Leaf Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Genetic Material ◽

Leaf Rust Resistance ◽

Wheat Varieties ◽

Major Resistance Genes ◽

Greenhouse Tests

Among the factors which determine yield reliability an important role is played by disease resistance. One of the breeding aims in the Martonvásár institute is to develop wheat varieties with resistance to major diseases. The winter wheat varieties bred in Martonvásár are examined in artificially inoculated nurseries and greenhouses for resistance to economically important pathogens. The effectiveness of designated genes for resistance to powdery mildew and leaf rust has been monitored over a period of several decades. None of the designated major resistance genes examined in greenhouse tests is able to provide complete resistance to powdery mildew; however, a number of leaf rust resistance genes provide full protection against pathogen attack (Lr9, Lr19, Lr24, Lr25, Lr28 and Lr35). In the course of marker-assisted selection, efficient resistance genes (Lr9, Lr24, Lr25 and Lr29) have been incorporated into Martonvásár wheat varieties. The presence of Lr1, Lr10, Lr26, Lr34 and Lr37 in the Martonvásár gene pool was identified using molecular markers. New sources carrying alien genetic material have been tested for powdery mildew and leaf rust resistance. Valuable Fusarium head blight resistance sources have been identified in populations of old Hungarian wheat varieties. Species causing leaf spots (Pyrenophora tritici-repentis, Septoria tritici and Stagonospora nodorum) have gradually become more frequent over the last two decades. Tests on the resistance of the host plant were begun in Martonvásár four years ago and regular greenhouse tests on seedlings have also been initiated.

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