Modern approach to the treatment of atopic dermatitis with preserved fetal liver cells (experimental study)

2021 ◽  
Vol 42 (2) ◽  
pp. 32-38
Author(s):  
L. A. Leonova ◽  
◽  
L. V. Ostankova ◽  
M. O. Bondarovych ◽  
M. V. Ostankov ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Immune System ◽  
Fetal Liver ◽  
Liver Cells ◽  
Biologically Active ◽  
Immunological Parameters ◽  
Modern Approach ◽  
Wide Range ◽  
Amplifying Effect ◽  
Fetal Liver Cells

One of the urgent problems of modern dermatology is atopic dermatitis (AD), which has multifactorial pathogenesis, the significant prevalence of the disease, the increased frequency of the complicated course, the lack of radical methods of therapy. The expediency to use cryopreserved fetal liver cells (cFLCs) for the treatment of AD is proved by a wide range of produced by them biologically active substances with immunomodulatory and anti-inflammatory activity. Disclosure of the mechanisms of the therapeutic action of biotherapeutic drugs in AD provides for the determination of the state of the cellular and humoral links of the immune system (IS). In this regard, the aim of the work was to assess the effectiveness of cFLCs injection by characteristic clinical and immunological parameters in rats with AD. The results of the study in rats with AD revealed disorders in the IS, manifested in a decrease in the total number of T-lymphocytes and their subpopulations in the spleen, in an increase in the level of circulating immune complexes and a number of immunoglobulins in the blood serum, and in a decrease in the phagocytic activity of the peritoneal cavity cells. Therapy with cFLCs, in contrast to the standard treatment with prednisolone, significantly improves the therapeutic effect, which is demonstrated by the restoration of the parameters of the cellular and humoral links of the immune system in animals with AD. The amplifying effect of the combined use of cFLCs and prednisolone on a number of parameters of the immune system in AD was shown.

Use of human fetal liver cells for treatment of patients with lower limb peripheral artery disease

2014 ◽  
Vol 2 (1) ◽  
pp. 10-13
Author(s):  
R. Salyutin ◽  
D. Dombrowski ◽  
M. Komarov ◽  
N. Sokolov ◽  
S. Palyanitsya ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Lower Limb ◽  
Fetal Liver ◽  
Life Quality ◽  
Liver Cells ◽  
Surgical Reconstruction ◽  
Clinical Effects ◽  
Doppler Flowmetry ◽  
Human Fetal Liver ◽  
Fetal Liver Cells

In the group of patients (n = 21, mean age 54 ± 5.8 years) with chronic lower limb ischemia stage IIB who were non-liable for reconstructiverestoration surgery, we have established positive clinical effects of local transplantation of human fetal liver progenitor cells. Complex examination following 1, 3, 6 and 12 months after transplantation included duplex scanning of limb arteries, x-ray contrast arteriography and laser Doppler flowmetry as well as measuring pain-free walking and evaluating life quality based on individual questionnaire data.Owing to the transplant “Cryopreserved human fetal liver progenitor cells” the patients demonstrated stable increase of life quality index and pain-free walking as well as improvement of general health allowing assign them to the group of patients with lower ischemia stage,  quicker social rehabilitation and lesser risk of disabling surgery (р < 0.05). Also, there were observations of improved microcirculation in the ischemic extremities owing to activation of endothelium-independent mechanisms of vasodilatation, reduced myotonus and neurotonus of the pre-capillaries and improved endothelium-dependent influence on the microhaemodynamic and, hence, an increased reserve capillary blood flow (p < 0.05).Analysis of the obtained results indicates prospects and effectiveness of using fetal liver cells transplantation in the patients who are not liable for surgical reconstruction of the vascular bed.

Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF β-receptor null mice

Blood ◽  
2001 ◽  
Vol 97 (7) ◽  
pp. 1990-1998 ◽  
Author(s):  
Wolfgang E. Kaminski ◽  
Per Lindahl ◽  
Nancy L. Lin ◽  
Virginia C. Broudy ◽  
Jeffrey R. Crosby ◽  
...  
Keyword(s):  
Growth Factor ◽  
Fetal Liver ◽  
Liver Cells ◽  
Platelet Derived Growth Factor ◽  
Wild Type ◽  
Liver Growth ◽  
Hematologic Disorders ◽  
Fetal Liver Cells ◽  
Β Receptor

Abstract Platelet-derived growth factor (PDGF)-B and PDGF β-receptor (PDGFRβ) deficiency in mice is embryonic lethal and results in cardiovascular, renal, placental, and hematologic disorders. The hematologic disorders are described, and a correlation with hepatic hypocellularity is demonstrated. To explore possible causes, the colony-forming activity of fetal liver cells in vitro was assessed, and hematopoietic chimeras were demonstrated by the transplantation of mutant fetal liver cells into lethally irradiated recipients. It was found that mutant colony formation is equivalent to that of wild-type controls. Hematopoietic chimeras reconstituted with PDGF-B−/−, PDGFRβ−/−, or wild-type fetal liver cells show complete engraftment (greater than 98%) with donor granulocytes, monocytes, B cells, and T cells and display none of the cardiovascular or hematologic abnormalities seen in mutants. In mouse embryos, PDGF-B is expressed by vascular endothelial cells and megakaryocytes. After birth, expression is seen in macrophages and neurons. This study demonstrates that hematopoietic PDGF-B or PDGFRβ expression is not required for hematopoiesis or integrity of the cardiovascular system. It is argued that metabolic stress arising from mutant defects in the placenta, heart, or blood vessels may lead to impaired liver growth and decreased production of blood cells. The chimera models in this study will serve as valuable tools to test the role of PDGF in inflammatory and immune responses.

scholarly journals Comparison of Basal Gene Expression and Induction of CYP3As in HepG2 and Human Fetal Liver Cells

2007 ◽  
Vol 30 (11) ◽  
pp. 2091-2097 ◽  
Author(s):  
Masataka Maruyama ◽  
Tamihide Matsunaga ◽  
Eri Harada ◽  
Shigeru Ohmori
Keyword(s):  
Gene Expression ◽  
Fetal Liver ◽  
Liver Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

scholarly journals Mechanisms of CYP3A Induction by Glucocorticoids in Human Fetal Liver Cells

2012 ◽  
Vol 27 (6) ◽  
pp. 653-657 ◽  
Author(s):  
Tamihide Matsunaga ◽  
Masataka Maruyama ◽  
Tsutomu Matsubara ◽  
Kiyoshi Nagata ◽  
Yasushi Yamazoe ◽  
...  
Keyword(s):  
Fetal Liver ◽  
Liver Cells ◽  
Human Fetal Liver ◽  
Fetal Liver Cells ◽  
Human Fetal Liver Cells

scholarly journals Hemoglobin switching in sheep: characteristics of BFU-E-derived colonies from fetal liver

Blood ◽  
1980 ◽  
Vol 56 (3) ◽  
pp. 495-500
Author(s):  
JE Barker
Keyword(s):  
Stem Cells ◽  
Fetal Liver ◽  
Fetal Hemoglobin ◽  
Liver Cells ◽  
S Phase ◽  
Hemoglobin Switching ◽  
Fetal Liver Cells ◽  
Beta 2 ◽  
Number Of Cells

Two types of erythroid colonies were generated in vitro from sheep fetal liver cells. The first type consisted of single colonies of 8–256 cells that were well hemoglobinized by 4 days; these are thought to originate from CFU-E. The second type consisted of macroscopic colonies composed of several subcolonies that matured between days 3 and 8 in vitro. At maturity, each contained 256 to > 1000 cells that formed a discrete macroscopic cluster. The macroscopic colonies, not previously described in sheep, are thought to be derived from BFU-E. The characteristics of sheep BFU-E were defined and the production of fetal hemoglobin (HbF, alpha 1, gamma 2) and HbC (alpha 2 beta 2) was compared in colonies derived from CFU-E or BFU-E. Bursts developed at erythropoietin (epo) concentrations as low as 0.1 U/ml, although the number observed increased with epo concentration up to 10 U/ml. The number of bursts observed was approximately proportional to the number of cells plated. As shown by thymidine suicide, approximately 50% of both the BFU e and CFU-E were in S-phase when obtained from the fetus. BFU-E were smaller and partially separable from CFU-E after sedimentation at unit gravity. The beta c/gamma synthetic ratio in colonies derived from BFU-E was greater than in CFU-E-derived colonies. These data suggest that the capacity for generation of erythroblasts making HbC is greater in the earlier or more primitive erythroid stem cells in fetal liver.

Expansion of mouse hematopoietic stem/progenitor cells in three-dimensional cocultures on growth-suppressed stromal cell layer

2019 ◽  
Vol 42 (7) ◽  
pp. 374-379 ◽  
Author(s):  
Hirotoshi Miyoshi ◽  
Chiaki Sato ◽  
Yuichiro Shimizu ◽  
Misa Morita
Keyword(s):  
Progenitor Cells ◽  
Mitomycin C ◽  
Stromal Cells ◽  
Fetal Liver ◽  
Three Dimensional ◽  
Hematopoietic Cells ◽  
Liver Cells ◽  
Hematopoietic Progenitor ◽  
Hematopoietic Stem ◽  
Fetal Liver Cells

With the aim of establishing an effective method to expand hematopoietic stem/progenitor cells for application in hematopoietic stem cell transplantation, we performed ex vivo expansion of hematopoietic stem/progenitor cells derived from mouse fetal liver cells in three-dimensional cocultures with stromal cells. In these cocultures, stromal cells were first cultured within three-dimensional scaffolds to form stromal layers and then fetal liver cells containing hematopoietic cells were seeded on these scaffolds to expand the hematopoietic cells over the 2 weeks of coculture in a serum-containing medium without the addition of cytokines. Prior to coculture, stromal cell growth was suppressed by treatment with the DNA synthesis inhibitor mitomycin C, and its effect on hematopoietic stem/progenitor cell expansion was compared with that in control cocultures in which fetal liver cells were cocultured with three-dimensional freeze-thawed stromal cells. After coculture with mitomycin C-treated stromal cells, we achieved a several-fold expansion of the primitive hematopoietic cells (c-kit+hematopoietic progenitor cells >7.8-fold, and CD34+hematopoietic stem/progenitor cells >3.5-fold). Compared with control cocultures, expansion of hematopoietic stem/progenitor cells tended to be lower, although that of hematopoietic progenitor cells was comparable. Thus, our results suggest that three-dimensional freeze-thawed stromal cells have higher potential to expand hematopoietic stem/progenitor cells compared with mitomycin C-treated stromal cells.

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