scholarly journals The role of adipose tissue in the healing of rat uterine wall after a full-thickness surgical incision

2021 ◽  
Vol 10 (4) ◽  
pp. 72-80
Author(s):  
N.B. Tikhonova ◽  
◽  
A.P. Milovanov ◽  
V.V. Aleksankina ◽  
T.V. Fokina ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Uterine Wall ◽  
Uterine Horn ◽  
Morphometric Study ◽  
Full Thickness ◽  
Sprague Dawley ◽  
Surgical Incision ◽  
Laboratory Rodents ◽  
The Right

Introduction. Data about the role of components of adipose tissue in the repair of damaged uterine walls are limited, although a number of authors claim that cell-based drugs from adipose tissue have a positive effect on the repair of damaged uterine walls in laboratory rodents. Meanwhile, stem cells derived from adipose tissue are considered to be the most promising type of cells in regenerative medicine. The aim of the studywas to evaluate the adipocyte components in the uterine wall of rats in healing after a full-thickness surgi-cal incision. Materials and methods. We conducted the study on 40 female Sprague Dawley rats. The animals were sub-jected to a full-thickness longitudinal incision in the wall of the right uterine horn, with the left one serving as an intact control. We carried out morphological examinations of the uterine walls daily in 5 animals from day 1 to 7 and on day 15. The sections from paraffin blocks were stained with hematoxylin and eosin and Mallory’s trichrome staining. Immunohistochemistry detected FABP4+ adipocytes and CD68+ macrophages. The morphometric study was carried out using the Leica system (Leica, Germany). The results underwent processing in the Statistica 12 software (StatSoft). Results. We noticed the period of the most active interaction of adipose tissue with the damaged horn to last from day 3 to 15 and coincide with the macrophage activation in the healing zone. The intact left uterine horn was not involved in the interaction processes with the mesenteric adipose tissue. Starting from day 3 FABP4+ cells in the uterine wall of the operated horn formed groups, creating rounded nest-like structures. Clusters of FABP4+ cells were localized in the healing zone, near the suture material, and in the perime-trium near the mesentery attachment sites. The changes over time of the indicators of the area of cell nests depended on the localization and duration of healing. There were no FABP4+ cells in the left intact horn. Conclusion. We characterized the morphological interaction of adipose tissue with the damaged uterine wall during the first two weeks after a full-thickness surgical incision of the rat uterine horn. The results of the study indicate that adipocytes take an active part in the healing after a surgical incision of the rat uterine wall at the earliest stages. Keywords: rat uterus, hysterotomy, healing, adipocytes, macrophages

Measurement of subscellular calcium distribution in cardiac muscle in the presence of caffeine and elevated extracellular calcium

1990 ◽  
Vol 48 (2) ◽  
pp. 170-171
Author(s):  
B. J. Hurley ◽  
M. E. Cantino
Keyword(s):  
Cardiac Muscle ◽  
Papillary Muscle ◽  
Myocardial Contraction ◽  
Extracellular Calcium ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Tension Transducer ◽  
The Right ◽  
Rat Papillary Muscle

Calcium is thought to play a major role in the activation of mammalian cardiac muscle. Several investigators have utilized Electron Probe X-ray Microanalysis (EPMA) to study the role of calcium in myocardial contraction. The purpose of this study is to measure subcellular elemental concentrations in rat papillary muscle under the influence of caffeine or elevated extracellular calcium. Of particular interest is the concentration of calcium in the junctional sarcoplasmic reticulum (jSR) under these conditions.Male Sprague-Dawley rats weighing approximately 300 g were anesthetized using I.P. Na-pentabarbitol injection. The hearts were carefully removed and back-perfused with a modified Tyrodes solution via cannulation of the aorta. The wall of the right ventricle was cut away revealing the papillary muscles. The tendon and wall ends of the papillary muscle were then sutured and the muscle was carefully cut out of the heart. The papillary muscle was then placed on the freezing device holder and the suture tied to a fixed post and tension transducer post, as shown in Figure 1.

Analysis of the rat uterine wall healing after a full thickness surgical incision

2021 ◽  
Vol 172 (7) ◽  
pp. 120-124
Author(s):  
N. B. Tikhonova ◽  
◽  
A. P. Milovanov ◽  
V. V. Aleksankina ◽  
T. V. Fokina ◽  
...  
Keyword(s):  
Uterine Wall ◽  
Full Thickness ◽  
Surgical Incision

Analysis of Healing of Rat Uterine Wall After Full-Thickness Surgical Incision

2021 ◽  
Author(s):  
N. B. Tikhonova ◽  
A. P. Milovanov ◽  
V. V. Aleksankina ◽  
T. V. Fokina ◽  
M. N. Boltovskaya ◽  
...  
Keyword(s):  
Uterine Wall ◽  
Full Thickness ◽  
Surgical Incision

scholarly journals Uterine torsion in a Sprague Dawley rat (Rattus norvegicus)

2011 ◽  
Vol 82 (3) ◽  
Author(s):  
K. H. Erlwanger ◽  
M. A. Costello ◽  
L. C. R. Meyer
Keyword(s):  
Histopathological Examination ◽  
Rare Condition ◽  
Hepatic Necrosis ◽  
Uterine Horn ◽  
Sprague Dawley ◽  
Metabolic Complications ◽  
Sprague Dawley Rat ◽  
Uterine Torsion ◽  
Lymphocytic Infiltrates ◽  
The Right

Uterine torsion is a twisting of the uterus or uterine horn perpendicular to its long axis.We report a case of uterine torsion in an adult breeding Sprague Dawley rat. The rat died a month after her last recorded delivery. Post mortem examination of the rat revealed 270° torsion of the right uterine horn. The uterus contained a single foetus. The liver was pale and enlarged. The rest of the viscera appeared normal. Histopathological examination showed acute hepatic necrosis and pulmonary congestion with mild lymphocytic infiltrates peribronchially. The acute hepatic necrosis may have been associated with septicaemia due to compromised blood vessels following the uterine torsion. The presence of a single foetus could have resulted in foeto-maternal disproportion with resultant uterine torsion. Torsion of the uterus can be accompanied by haemostatic and metabolic complications, which could have caused the death of the rat. Although uterine torsion is a rare condition in rats, it should be considered as a potential complication of gestation in animal breeding units.

scholarly journals P821 Influence of periplaque fat on coronary plaque vulnerability, a comparative analysis between atherosclerotic lesions located in the right versus left coronary arteries

2020 ◽  
Vol 21 (Supplement_1) ◽  
Author(s):  
N Rat ◽  
D Opincariu ◽  
R Hodas ◽  
M Ratiu ◽  
A Mester ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Comparative Analysis ◽  
Coronary Artery ◽  
Coronary Plaque ◽  
Plaque Vulnerability ◽  
Atherosclerotic Lesions ◽  
Group 2 ◽  
The Right ◽  
Group 1

Abstract Funding Acknowledgements Research grant PlaqueImage, contract number 26/01.09.2016, SMIS code 103544, Project funded by the European Union and the Government of Romania Background The role of epicardial adipose tissue on coronary plaque vulnerability has been well established. However, the role of periplaque fat (PPF) has not been elucidated so far. Moreover, there is scarce data on the role of plaque location, in relation to peri-atheromatous adipose tissue on the vulnerability degree and morphology of coronary atherosclerotic lesions. Purpose To evaluate the influence of PPF on coronary plaque vulnerability, in a comparative analysis between atherosclerotic lesions located in the right and left coronary arteries. Methods This is an observational study which included 82 patients with stable CAD, who underwent 128-multislice CT coronary angiography, presented at least one coronary lesion with at least 50% degree of stenosis and exhibited ≥1 vulnerability markers in the respective coronary plaque. Plaques presenting features of vulnerability (spotty calcifications – SC, Napkin ring sign – NRS, low attenuation plaque – LAP, positive remodeling – PR), were defined as vulnerable plaques (VP). Image postprocessing was performed with the Syngo.via Frontier software and PPF was measured 10 mm around the analyzed VP. Based to the plaque location within the coronary tree, the study subjects were divided into: group 1 (location of VP in the right coronary artery - RCA) - n = 17; group 2 (location of VP in the left coronary artery - LCA) - n = 65. Results The analysis of the plaque characteristics indicated that the VPs from the RCA were significantly longer (20.81± 6.45 vs. 17.37 ± 4.59 mm, p = 0.02) and had a larger volume (269.3± 120.4 vs. 161.6 ± 80.89 mm3, p < 0.0001) compared to the VPs from the LCA. Compared to group 2, coronary plaques in group 1 exhibited a higher vulnerability degree, illustrated by a larger non-calcified volume (232.5 ± 111 vs. 134.5 ± 83.29 mm3, p = 0.0006), lipid-rich volume (19.4 ± 19.07 vs. 10.27 ± 17.08 mm3, p = 0.0106), and fibro-fatty volume (213 ± 101.3 vs. 124.2 ± 7.98 mm3, p = 0.0009). The PPF was significantly larger in VPs from the RCA (0.92 ± 0.48 mm3 vs. 0. 57± 0.34 mm3, p = 0.0041) compared to VPs located in the LCA. No differences were found regarding the total epicardial fat between the two groups (p = 0.386). Conclusions Atherosclerotic plaques located in the RCA exhibited a higher number of vulnerability characteristics compared to those located in the left coronary system and PPF was more pronounced in the regions surrounding VPs located within the RCA. This difference in vulnerability features could be explained not only by geometrical and hemodynamical characteristics of the coronary circulation, but also by inflammation-mediated alteration of endothelial shear stress triggered by release of inflammatory mediators from the local epicardial fat.

scholarly journals Fatal Placental Subinvolution in a Captive Capybara (Hydrochaeris hydrochaeris, Order Rodentia)

2005 ◽  
Vol 42 (4) ◽  
pp. 513-516
Author(s):  
C. Juan-Sallés ◽  
L. S. Martínez ◽  
M. M. Garner
Keyword(s):  
Blood Clot ◽  
Abdominal Cavity ◽  
Uterine Wall ◽  
Uterine Horn ◽  
Vascular Lesions ◽  
Coagulative Necrosis ◽  
Small Perforation ◽  
The Right ◽  
The Masses ◽  
Hydrochaeris Hydrochaeris

An adult, captive-born female capybara died of systemic thrombosis and hemoperitoneum associated with placental subinvolution. Grossly, the uterus was enlarged, segmentally thickened, and associated with a large blood clot in the abdominal cavity. There was hemometra and a large ovoid mass in each uterine horn weakly adhered to the endometrium, and the right uterine horn wall had a small perforation over the mass. The mesometrial veins were markedly dilated due to thrombosis and occasionally perforated. Histologically, the uterine masses consisted of partly necrotic placental and subplacental tissue. The uterine wall surrounding the masses had full-thickness coagulative necrosis of the myometrium and diffuse endometrial ulceration with abundant syncytiotrophoblast-like cells within capillaries. Vascular lesions in the uterus and mesometrium consisted of mural invasion by cytotrophoblast and syncytiotrophoblast-like cells, thrombosis, fibrinoid necrosis, and/or heterophilic vasculitis. This is the first report of placental subinvolution in capybaras or any rodent species, to the authors' knowledge.

Resorption of Residual Ridges (RRR) in Rats

1987 ◽  
Vol 66 (12) ◽  
pp. 1753-1757 ◽  
Author(s):  
I. Nishimura ◽  
P.J. Damiani ◽  
D.A. Atwood
Keyword(s):  
Bone Resorption ◽  
Alveolar Bone ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Minimal Trauma ◽  
Specific Factors ◽  
Maxilla And Mandible ◽  
The Right ◽  
Mandibular First Molar

The post-extraction resorption of residual ridges (RRR) is a major and largely unsolved health problem, probably of multifactorial etiology. In order for one to study the role of specific factors in the pathophysiology of RRR, the use of animal models is desirable. The purpose of this study was to establish a reliable animal model and a standardized assay system to measure RRR for future experiments. A new oblique cephalometric device was designed to take pairs of xeroradiographs on the right and left sides of the rat skull and mandible at a 45-degree angle to the horizontal plane. Preliminary studies confirmed the reproducibility of the technique. All molars were extracted with minimal trauma from the right maxilla and mandible in five male Sprague-Dawley rats (40 days old). Longitudinal cephalographic examinations were performed before and immediately after extraction and at two, four, eight, and 12 weeks after extraction. Alveolar bone resorption was measured on enlarged cephalographs (5. 7X) at a point mesial to the mandibular first molar. Sequential mean bone resorption was 0.8 ± 0.2 mm (S.D.), 1.0 ± 0.3, 1.3 ± 0.3, and 1.5 ± 0.4, respectively. Graphically, these findings produced bone loss curves similar to those observed in man. These results indicate that the rat model may be utilized in longitudinal studies of the resorption of the residual ridge.

scholarly journals Role of Red Blood Cell Lysis and Iron in Hydrocephalus after Intraventricular Hemorrhage

2014 ◽  
Vol 34 (6) ◽  
pp. 1070-1075 ◽  
Author(s):  
Chao Gao ◽  
Hanjian Du ◽  
Ya Hua ◽  
Richard F Keep ◽  
Jennifer Strahle ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Intraventricular Hemorrhage ◽  
Intraventricular Injection ◽  
Heme Oxygenase 1 ◽  
Ventricular Enlargement ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
The Right

Thrombin and iron are two major players in intracerebral hemorrhage-induced brain injury and our recent study found that thrombin contributes to hydrocephalus development in a rat model of intraventricular hemorrhage (IVH). This study investigated the role of red blood cell (RBC) lysis and iron in hydrocephalus after IVH. There were three parts to this study. First, male Sprague-Dawley rats received an injection of saline, packed, or lysed RBCs into the right lateral ventricle. Second, rats had an intraventricular injection of iron or saline. Third, the rats received intraventricular injection of lysed RBCs mixed with deferoxamine (0.5 mg in 5 μL saline) or saline. All rats underwent magnetic resonance imaging at 24 hours and were then euthanized for brain edema measurement, western blot analysis, or brain histology. We found that intraventricular injection of lysed RBCs, but not packed RBCs, resulted in ventricular enlargement and marked increases in brain heme oxygenase-1 and ferritin at 24 hours. Intraventricular injection of iron also resulted in ventricular enlargement and ventricular wall damage 24 hours later. Coinjection of deferoxamine reduced lysed RBC-induced ventricular enlargement ( P<0.01). These results suggest that iron, a degradation product of hemoglobin, has an important role in hydrocephalus development after IVH.

Surface ultrastructure of danazol treated rat endometrium: A SEM study

1983 ◽  
Vol 41 ◽  
pp. 556-557
Author(s):  
R.P. Apkarian ◽  
J.S. Sanfilippo
Keyword(s):  
Cross Sections ◽  
Reproductive Tract ◽  
Preliminary Investigation ◽  
Breast Disease ◽  
Distal Segment ◽  
Uterine Horn ◽  
Female Reproductive Tract ◽  
Ultrastructural Changes ◽  
Cycle Phase ◽  
Sprague Dawley

The synthetic androgen danazol, is an isoxazol derivative of ethisterone. It is utilized in the treatment of endometriosis, fibrocystic breast disease, and has a potential use as a contraceptive. A study was designed to evaluate the ultrastructural changes associated with danazol therapy in a rat model. The preliminary investigation of the distal segment of the rat uterine horn was undertaken as part of a larger study intended to elucidate the effects of danazol on the female reproductive tract.Cross-sections (2-3 mm in length) of the distal segment of the uterine horn from sixteen Sprague-Dawley rats were prepared for SEM. Ten rats in estrus served as controls and six danazol treated rats were noted to have alterations of the estrus cycle i.e. a lag in cycle phase or noncycling patterns. Specimens were fixed in 3% glutaraldehyde in 0.05M phosphate buffer containing CaCl2 at pH 7.0-7.4 and chilled to 4°C. After a brief wash in distilled water, specimens were passed through a graded series of ethanol, critical point dryed in CO2 from absolute ethanol, and coated with 6nm Au. Observations were made with an IS1-40 SEM operated at 15kV.

Sign in / Sign up

Export Citation Format

Share Document