High Prevalence of Hemoglobin Disorders and Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency in the Republic of Guinea (West Africa)

Hemoglobin ◽  
2011 ◽  
Vol 36 (1) ◽  
pp. 25-37 ◽  
Author(s):  
Tamba S. Millimono ◽  
Kovana M. Loua ◽  
Silvia L. Rath ◽  
Luis Relvas ◽  
Celeste Bento ◽  
...  
Blood ◽  
1993 ◽  
Vol 81 (8) ◽  
pp. 2150-2154 ◽  
Author(s):  
DT Chiu ◽  
L Zuo ◽  
L Chao ◽  
E Chen ◽  
E Louie ◽  
...  

Abstract The underlying DNA changes associated with glucose-6-phosphate dehydrogenase (G6PD)-deficient Asians have not been extensively investigated. To fill this gap, we sequenced the G6PD gene of 43 G6PD- deficient Chinese whose G6PD was well characterized biochemically. DNA samples were obtained from peripheral blood of these individuals for sequencing using a direct polymerase chain reaction (PCR) sequencing procedure. From these 43 samples, we have identified five different types of nucleotide substitutions in the G6PD gene: at cDNA 1388 from G to A (Arg to His); at cDNA 1376 from G to T (Arg to Leu); at cDNA 1024 from C to T (Leu to Phe); at cDNA 392 from G to T (Gly to Val); at cDNA 95 from A to G (His to Arg). These five nucleotide substitutions account for over 83% of our 43 G6PD-deficient samples and these substitutions have not been reported in non-Asians. The substitutions found at cDNA 392 and cDNA 1024 are new findings. The substitutions at cDNA 1376 and 1388 account for over 50% of the 43 samples examined indicating a high prevalence of these two alleles among G6PD-deficient Chinese. Our findings add support to the notion that diverse point mutations may account largely for much of the phenotypic heterogeneity of G6PD deficiency.


1977 ◽  
Vol 26 (4) ◽  
pp. 756-764 ◽  
Author(s):  
J. G. Breman ◽  
J. M. Lane ◽  
A. B. Alécaut

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257562
Author(s):  
Linda Eva Amoah ◽  
Kwame Kumi Asare ◽  
Donu Dickson ◽  
Joana Abankwa ◽  
Abena Busayo ◽  
...  

Introduction The global effort to eradicate malaria requires a drastic measure to terminate relapse from hypnozoites as well as transmission via gametocytes in malaria-endemic areas. Primaquine has been recommended for the treatment of P. falciparum gametocytes and P. vivax hypnozoites, however, its implementation is challenged by the high prevalence of G6PD deficient (G6PDd) genotypes in malaria endemic countries. The objective of this study was to profile G6PDd genotypic variants and correlate them with malaria prevalence in Ghana. Methods A cross-sectional survey of G6PDd genotypic variants was conducted amongst suspected malaria patients attending health care facilities across the entire country. Malaria was diagnosed using microscopy whilst G6PD deficiency was determined using restriction fragment length polymorphisms at position 376 and 202 of the G6PD gene. The results were analysed using GraphPad prism. Results A total of 6108 subjects were enrolled in the study with females representing 65.59% of the population. The overall prevalence of malaria was 36.31%, with malaria prevalence among G6PDd genotypic variants were 0.07% for A-A- homozygous deficient females, 1.31% and 3.03% for AA- and BA- heterozygous deficient females respectively and 2.03% for A- hemizygous deficient males. The odd ratio (OR) for detecting P. falciparum malaria infection in the A-A- genotypic variant was 0.0784 (95% CI: 0.0265–0.2319, p<0.0001). Also, P. malariae and P. ovale parasites frequently were observed in G6PD B variants relative to G6PD A- variants. Conclusion G6PDd genotypic variants, A-A-, AA- and A- protect against P. falciparum, P. ovale and P. malariae infection in Ghana.


2021 ◽  
Vol 14 (6.) ◽  
pp. 8-43
Author(s):  
Takehiko Ochiai

This article aims to examine how Matacong Island, a small island just off the coast of the Republic of Guinea, West Africa, was claimed its possession by local chiefs, how it was leased to and was used by European and Sierra Leonean merchants, and how it was colonized by Britain and France in the 19th century. In 1825 the paramount chief of Moriah chiefdom agreed to lease the island to two Sierra Leonean merchants, and in 1826 it was ceded to Britain by a treaty with chiefs of the Sumbuyah and Moriah chiefdoms. Since the island was considered as a territory exempted from duty, British and Sierra Leonean merchants used it as an important trading station throughout the 19th century. Major exports of Matacong Island included palm kernels, palm oil, hides, ivory, pepper and groundnuts, originally brought by local traders from the neighboring rivers, and major imports were tobacco, beads, guns, gunpowder, rum, cotton manufactures, iron bars and hardware of various kinds. In 1853 alone, some 80 vessels, under British, American, and French flags, anchored at Matacong Island. By the convention of 1882, Britain recognized the island as belonging to France. Although the convention was never ratified, it was treated by both countries as accepted terms of agreement. The article considers various dynamics of usage, property, and territorial possession as relates to the island during the 19th century, and reveals how complex they were, widely making use of the documents of The Matacong Island (West Africa) Papers at the University of Birmingham Library in Britain. The collection purchased by the library in 1969 is composed of 265 historical documents relating to Matacong Island, such as letters, agreements, newspaper-cuttings, maps and water-color picture


2020 ◽  
Author(s):  
Wonsig Lee ◽  
Sang-Eun Lee ◽  
Min Jun Lee ◽  
Kyung Tae Noh

Abstract Background Glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most prevalent inborn disorder. This X-chromosome-linked recessive disease affects more than 400 million people globally, and is associated with haemolytic anaemia after medication with the anti-latent malaria drug, primaquine. To prevent malaria, the Republic of Korea (ROK) Army administers malaria chemoprophylaxis. Due to the previously low G6PD deficiency prevalence in the ROK, prior to primaquine administration, testing for G6PD deficiency was not mandatory. In this study, to evaluate the risk from malaria chemoprophylaxis in the ROK, G6PD deficiency prevalence was investigated. Methods Blood specimens from 1,632 soldiers entering training camp for the 3 rd Infantry of the ROK Army were collected. CareStart™ Biosensor for G6PD and haemoglobin (Hb) was used to detect G6PD levels. G6PD variants using the DiaPlexC G6PD Genotyping kit (Asian type) and full-length sequencing were examined. Results Of 1,632 blood specimens tested, none was observed to be G6PD deficient. The median value of all tested samples was 7.582 U/g Hb. An investigation of 170 G6PD DNA variants was analysed and categorized as partially low normal [n = 131, 30–80% (2.27–6.05 U/g Hb)of the median value], high [n=3, >150% (>11.373 U/g Hb) of the median value], or normal [n = 36, 80–150% (6.05–11.373 U/g Hb) of the median value], and none was amplified by the DiaPlexC kit. Five silent mutations (C→T) in 38 partially abnormal specimens were found at the 1,311th nucleotide position by sequence analysis. Another 8 silent mutations (T93C) were also detected in 131 partially low normal specimens. Thus, it is inferred that these silent mutations could be related to G6PD activity. Conclusions This G6PD deficiency prevalence study, conducted among participants from the 3 rd Infantry of the ROK Army, provided crucial evidence for the safety of malaria chemoprophylaxis. This study showed found that the prevalence of G6PD deficiency among 1,632 young soldiers was wholly absent. Although G6PD phenotypic mutations were not detected, many silent mutations (C1311T and T93C) were observed. Thus, it is inferred that malaria chemoprophylaxis is relatively safe against G6PD deficiency-mediated haemolytic anaemia. However, given the number of individuals whose G6PD were at the low end of the normal range and the frequent detection of G6PD deficiency-related mutations, consistent monitoring of G6PD deficiency is needed. Keywords : Glucose-6-phosphate dehydrogenase deficiency, Prevalence, Single nucleotide polymorphism, Primaquine


2019 ◽  
Vol 31 (3) ◽  
pp. 226-232

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas, affecting more than 400 million people worldwide. This hospital- and laboratory-based, cross-sectional descriptive study was conducted with the aim of determining the prevalence of G6PD deficiency among 200 newborns at 300-bedded Pyin Oo Lwin General Hospital during January to March 2017. The participants were 103 girls (58.5%) and 97 boys (41.5%). Both qualitative and quantitative measurements by using Brewer's method and G-SIX kit method were applied for diagnosis of G6PD deficiency. Total serum bilirubin level was measured by Bilirubinometer. Of the 200 newborns, 21(10.5%) were G6PD deficient. The overall prevalence of G6PD deficiency was 10.5% (21/200) and male was predominant than female (17.5% vs 3.9%). Out of 10.5% (21/100)G6PD deficient newborns, 5(23.8%) and 16(76.2%) were mild and moderate G6PD deficiency, respectively. Regarding hyperbilirubinaemia, 9(42.9%), 3(14.3%), 2(19.0%) and 5(23.8%) were severe, moderate and mild hyperbilirubinaemia and normal bilirubin, respectively. This study showed that a significant correlation between the severity of hyperbili- rubinaemia and G6PD activity (p <0.05). Taking into consideration of the above results, the high prevalence can be useful for providing appropriate prevention and early treatment of complications in routine neonatal screening in this area.


Oryx ◽  
2012 ◽  
Vol 46 (2) ◽  
pp. 253-259 ◽  
Author(s):  
David Brugière

AbstractThe Republic of Guinea has one of the highest diversities of mammal species in West Africa. However, its protected area network is poorly developed and little quantitative information has been available to help guide national conservation strategies. I therefore examined the distribution of antelopes and related species (families Bovidae and Tragulidae) across 17 sites, including four protected areas, to determine how the existing protected area network contributes to the conservation of antelope species and where action should best be focused for the conservation of this group. A total of 21 species of antelope have been recorded in the 17 sites; four of these species are absent from the four protected areas. An iterative heuristic complementarity approach was used to determine an irreplaceability index, which accounts for both species richness and species rarity, for each of the sites. The Kankan Faunal Reserve and Nimba Strict Nature Reserve have the second and fourth highest irreplaceability indices, respectively. The two other protected areas have moderate to very low irreplaceability indices, showing that they protect species widespread throughout the 17 sites. The Ziama Forest has the highest index (because it contains a high number of species and of globally threatened species), highlighting the significance of this site. I discuss the importance of the other sites and the threats affecting antelopes in Guinea, and make recommendations to improve the study and conservation of antelope species in the country.


2020 ◽  
Author(s):  
Jorge da Rocha ◽  
Houcemeddine Othman ◽  
Caroline T. Tiemessen ◽  
Gerrit Botha ◽  
Michèle Ramsay ◽  
...  

AbstractChloroquine/hydroxychloroquine have been proposed as potential treatments for COVID-19. These drugs have warning labels for use in individuals with glucose-6-phosphate dehydrogenase (G6PD) deficiency. Analysis of whole-genome sequence data of 458 individuals from sub-Saharan Africa showed significant G6PD variation across the continent. We identified nine variants, of which four are potentially deleterious to G6PD function, and one (rs1050828) that is known to cause G6PD deficiency. We supplemented data for the rs1050828 variant with genotype array data from over 11,000 Africans. Although this variant is common in Africans overall, large allele frequency differences exist between sub-populations. African sub-populations in the same country can show significant differences in allele frequency (e.g. 16.0% in Tsonga vs 0.8% in Xhosa, both in South Africa, p = 2.4 × 10−3). The high prevalence of variants in the G6PD gene found in this analysis suggests that it may be a significant interaction factor in clinical trials of chloroquine and hydrochloroquine for treatment of COVID-19 in Africans.


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