scholarly journals Genotypic glucose-6-phosphate dehydrogenase (G6PD) deficiency protects against Plasmodium falciparum infection in individuals living in Ghana

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0257562
Author(s):  
Linda Eva Amoah ◽  
Kwame Kumi Asare ◽  
Donu Dickson ◽  
Joana Abankwa ◽  
Abena Busayo ◽  
...  
Keyword(s):  
G6pd Deficiency ◽  
Malaria Prevalence ◽  
Health Care Facilities ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Length Polymorphisms ◽  
G6pd Gene ◽  
High Prevalence ◽  
Care Facilities ◽  
Glucose 6 Phosphate Dehydrogenase

Introduction The global effort to eradicate malaria requires a drastic measure to terminate relapse from hypnozoites as well as transmission via gametocytes in malaria-endemic areas. Primaquine has been recommended for the treatment of P. falciparum gametocytes and P. vivax hypnozoites, however, its implementation is challenged by the high prevalence of G6PD deficient (G6PDd) genotypes in malaria endemic countries. The objective of this study was to profile G6PDd genotypic variants and correlate them with malaria prevalence in Ghana. Methods A cross-sectional survey of G6PDd genotypic variants was conducted amongst suspected malaria patients attending health care facilities across the entire country. Malaria was diagnosed using microscopy whilst G6PD deficiency was determined using restriction fragment length polymorphisms at position 376 and 202 of the G6PD gene. The results were analysed using GraphPad prism. Results A total of 6108 subjects were enrolled in the study with females representing 65.59% of the population. The overall prevalence of malaria was 36.31%, with malaria prevalence among G6PDd genotypic variants were 0.07% for A-A- homozygous deficient females, 1.31% and 3.03% for AA- and BA- heterozygous deficient females respectively and 2.03% for A- hemizygous deficient males. The odd ratio (OR) for detecting P. falciparum malaria infection in the A-A- genotypic variant was 0.0784 (95% CI: 0.0265–0.2319, p<0.0001). Also, P. malariae and P. ovale parasites frequently were observed in G6PD B variants relative to G6PD A- variants. Conclusion G6PDd genotypic variants, A-A-, AA- and A- protect against P. falciparum, P. ovale and P. malariae infection in Ghana.

scholarly journals Molecular characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency in patients of Chinese descent and identification of new base substitutions in the human G6PD gene

Blood ◽  
1993 ◽  
Vol 81 (8) ◽  
pp. 2150-2154 ◽  
Author(s):  
DT Chiu ◽  
L Zuo ◽  
L Chao ◽  
E Chen ◽  
E Louie ◽  
...  
Keyword(s):  
G6pd Deficiency ◽  
Point Mutations ◽  
Nucleotide Substitutions ◽  
New Findings ◽  
G6pd Gene ◽  
High Prevalence ◽  
Chinese Descent ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Sequencing Procedure

Abstract The underlying DNA changes associated with glucose-6-phosphate dehydrogenase (G6PD)-deficient Asians have not been extensively investigated. To fill this gap, we sequenced the G6PD gene of 43 G6PD- deficient Chinese whose G6PD was well characterized biochemically. DNA samples were obtained from peripheral blood of these individuals for sequencing using a direct polymerase chain reaction (PCR) sequencing procedure. From these 43 samples, we have identified five different types of nucleotide substitutions in the G6PD gene: at cDNA 1388 from G to A (Arg to His); at cDNA 1376 from G to T (Arg to Leu); at cDNA 1024 from C to T (Leu to Phe); at cDNA 392 from G to T (Gly to Val); at cDNA 95 from A to G (His to Arg). These five nucleotide substitutions account for over 83% of our 43 G6PD-deficient samples and these substitutions have not been reported in non-Asians. The substitutions found at cDNA 392 and cDNA 1024 are new findings. The substitutions at cDNA 1376 and 1388 account for over 50% of the 43 samples examined indicating a high prevalence of these two alleles among G6PD-deficient Chinese. Our findings add support to the notion that diverse point mutations may account largely for much of the phenotypic heterogeneity of G6PD deficiency.

Screening of Glucose-6-Phosphate Dehydrogenase Deficiency in Neonatal Hyperbilirubinaemia at 300-Bedded Pyin Oo Lwin General Hospital

2019 ◽  
Vol 31 (3) ◽  
pp. 226-232
Keyword(s):  
General Hospital ◽  
G6pd Deficiency ◽  
Serum Bilirubin Level ◽  
Total Serum ◽  
Cross Sectional ◽  
Qualitative And Quantitative ◽  
Quantitative Measurements ◽  
Human Enzyme ◽  
High Prevalence ◽  
Glucose 6 Phosphate Dehydrogenase

Glucose-6-phosphate dehydrogenase (G6PD) deficiency is one of the most common human enzyme deficiencies in the world. It is particularly common in populations living in malaria-endemic areas, affecting more than 400 million people worldwide. This hospital- and laboratory-based, cross-sectional descriptive study was conducted with the aim of determining the prevalence of G6PD deficiency among 200 newborns at 300-bedded Pyin Oo Lwin General Hospital during January to March 2017. The participants were 103 girls (58.5%) and 97 boys (41.5%). Both qualitative and quantitative measurements by using Brewer's method and G-SIX kit method were applied for diagnosis of G6PD deficiency. Total serum bilirubin level was measured by Bilirubinometer. Of the 200 newborns, 21(10.5%) were G6PD deficient. The overall prevalence of G6PD deficiency was 10.5% (21/200) and male was predominant than female (17.5% vs 3.9%). Out of 10.5% (21/100)G6PD deficient newborns, 5(23.8%) and 16(76.2%) were mild and moderate G6PD deficiency, respectively. Regarding hyperbilirubinaemia, 9(42.9%), 3(14.3%), 2(19.0%) and 5(23.8%) were severe, moderate and mild hyperbilirubinaemia and normal bilirubin, respectively. This study showed that a significant correlation between the severity of hyperbili- rubinaemia and G6PD activity (p <0.05). Taking into consideration of the above results, the high prevalence can be useful for providing appropriate prevention and early treatment of complications in routine neonatal screening in this area.

scholarly journals KNOWLEDGE AND PERCEPTION OF CAUSES OF GROSS HAEMATURIA AMONG THE INHABITANTS OF KWALKWALAWA RIVERINE SETTLEMENT OF SOKOTO

2017 ◽  
Vol 5 (8) ◽  
pp. 1-10
Author(s):  
Isah ◽  
Musa Giro ◽  
Yahaya ◽  
Awosan ◽  
Ibrahim ◽  
...  
Keyword(s):  
Democratic Republic Of Congo ◽  
Life Time ◽  
Knowledge Score ◽  
Health Care Facilities ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
High Prevalence ◽  
United Republic Of Tanzania ◽  
Domestic Use ◽  
Gross Haematuria

Blood in the urine is a common condition seen in people living around the riverine area which may result from bleeding anywhere along the urinary tract. Schistosomiasis is considered to be the commonest cause of haematuria among the inhabitants of riverine areas. Urinary schistosomiasis affects more than 200 million people worldwide and poses a threat to 600 million in more than 76 countries (WHO, 1993). In Africa alone, about 192 million cases of schistosomiasis have been reported; with Nigeria ranking the highest (15% of cases) followed by the United Republic of Tanzania (about 10% of the cases) and the Democratic Republic of Congo and Ghana with 7-8% each. A descriptive cross sectional survey was conducted among the inhabitants of Kwalkwalawa village in Wamakko local government Sokoto state, Nigeria. Majority of the respondents (81.4%) had passed bloody urine at one time of their life-time, unfortunately only 3.1% of the affected respondents had ever being to Hospital for treatment. Only 5.7% relates hematuria to contact with river water and the Knowledge score of gross hematuria revealed that only 11.4% have good knowledge of the disease. Perceived Social determinants of the disease in the environment include; Farming, Fishing, washing and Bathing in the River as well as fetching for Domestic use. The lack of proper knowledge of the cause of the disease and insufficient safe water supplies coupled with inadequate health care facilities may have influenced the infection rate and distribution of the disease in the area. In conclusion the study was able to find out a high prevalence of hematuria among the inhabitants of this study area, however, there is poor knowledge of the cause of bloody urine. Most of the respondents believed that bloody urine can be cured and prevented, but few relate it to drug treatment.

scholarly journals Perceptions of Healthcare Professionals on Covid-19 in Lesotho: a Cross-sectional Survey

2021 ◽  
Author(s):  
Isabel Nyangu ◽  
Maseabata Ramathebane
Keyword(s):  
Health Care ◽  
Health Care Providers ◽  
Healthcare Professionals ◽  
Survey Design ◽  
Well Being ◽  
Health Care Facilities ◽  
Care Providers ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Care Facilities

Abstract BackgroundHealth care professionals at the frontline of the Covid-19 pandemic require continued support to ensure not only their well-being, but enhanced care for those infected by the disease.AimThe aim of this study was to assess the perceptions of health care providers regarding the Covid-19 pandemic in Lesotho. MethodologyA quantitative cross-sectional survey design was used to collect data from a sample of 190 health care workers who were conveniently sampled from a total of 25 health care facilities (12 hospitals and 13 health center clinics). The sample included nurses, doctors, pharmacists, laboratory scientists/ technicians, dentists, counsellors, and administrative assistants. A total of 255 structured questionnaires were distributed and 190 of them were returned completed (75% response rate). Data were collected in September 2021 and analyzed using SPSS (vs23). Permission to conduct this study was granted by the relevant authorities. Informed written consent was given by the participants in writing before they could take part in this study.ResultsThe level of education was found to be associated with positive perceptions of the respondents on the Covid-19 pandemic. Respondents generally had positive views about the Covid-19 pandemic. Their views were positive as they understood that Covid-19 can affect anyone including themselves and is not a disease for any particular race. They were also aware that they could contract Covid-19, even despite pre-existing conditions. Respondents perceived that there was a positive prognosis after contracting Covid-19 if necessary precautions were taken. They had positive perceptions as they knew that the virus was affecting many people worldwide, including the elderly, and were willing to take the test and did not mind having to pay for it if asked to do so. Even though the Covid-19 test was viewed not to take too much time, respondents felt that it is rather painful and uncomfortable. The test providers were perceived as friendly and not rude, and respondents did not have any preferred gender for the test providers. Respondents were also willing to discuss issues on Covid-19 with families and friends. Majority of the respondents lived close to health care facilities.ConclusionsThis study concludes that health care providers at the frontline were mostly nurses. Positive perceptions about the Covid-19 pandemic in Lesotho were found as respondents were knowledgeable, and were willing to test for the disease. Test providers were viewed as pleasant and health care facilities were accessible. This shows positive progress towards fighting the pandemic in Lesotho.RecommendationsSupport for frontline healthcare professionals remains crucial to ensure effective service delivery. More education can be provided to improve the general views about the Covid-19 to improve uptake of services not only amongst healthcare professionals but the general public as well.

scholarly journals Molecular characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency in patients of Chinese descent and identification of new base substitutions in the human G6PD gene

Blood ◽  
1993 ◽  
Vol 81 (8) ◽  
pp. 2150-2154
Author(s):  
DT Chiu ◽  
L Zuo ◽  
L Chao ◽  
E Chen ◽  
E Louie ◽  
...  
Keyword(s):  
G6pd Deficiency ◽  
Point Mutations ◽  
Nucleotide Substitutions ◽  
New Findings ◽  
G6pd Gene ◽  
High Prevalence ◽  
Chinese Descent ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Sequencing Procedure

The underlying DNA changes associated with glucose-6-phosphate dehydrogenase (G6PD)-deficient Asians have not been extensively investigated. To fill this gap, we sequenced the G6PD gene of 43 G6PD- deficient Chinese whose G6PD was well characterized biochemically. DNA samples were obtained from peripheral blood of these individuals for sequencing using a direct polymerase chain reaction (PCR) sequencing procedure. From these 43 samples, we have identified five different types of nucleotide substitutions in the G6PD gene: at cDNA 1388 from G to A (Arg to His); at cDNA 1376 from G to T (Arg to Leu); at cDNA 1024 from C to T (Leu to Phe); at cDNA 392 from G to T (Gly to Val); at cDNA 95 from A to G (His to Arg). These five nucleotide substitutions account for over 83% of our 43 G6PD-deficient samples and these substitutions have not been reported in non-Asians. The substitutions found at cDNA 392 and cDNA 1024 are new findings. The substitutions at cDNA 1376 and 1388 account for over 50% of the 43 samples examined indicating a high prevalence of these two alleles among G6PD-deficient Chinese. Our findings add support to the notion that diverse point mutations may account largely for much of the phenotypic heterogeneity of G6PD deficiency.

scholarly journals A country-wide evaluation of infection control for tuberculosis in health-care facilities in Armenia

2019 ◽  
Vol 13 (05.1) ◽  
pp. 035S-041S
Author(s):  
Joshua Chadwick Jayaraj ◽  
Karapet Davtyan ◽  
Hayk Davatyan ◽  
Anush Khachatryan ◽  
Armen Hayrepetyan ◽  
...  
Keyword(s):  
Health Care ◽  
Infection Control ◽  
Health Care Facilities ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Incentive Model ◽  
Care Facilities ◽  
Control Programmes ◽  
Who Checklist ◽  
Primary Care Doctors

Introduction: Infection control at health facilities is an important part of TB control programmes. To assess the implementation of tuberculosis infection control (TB IC) measures and barriers hindering their implementation in TB health care facilities (HCFs) in Armenia; to report the feasibility of using the WHO recommended checklist. Methodology: A cross-sectional survey using WHO TB-IC checklist and direct observations was conducted between January and May 2018. Results: The survey included all 62 TB institutions in Armenia. TB HCFs in Armenia had implemented some recommended TB IC measures: offering IC training to staff (48%), shortening time to diagnosing TB to less than one day (29%) and ensuring good ventilation (60%). N95 respirators were available in all HCFs. However, barriers that hindered implementation of TB IC measures were: lack of training, a different incentive model for primary care doctors versus TB doctors and lack of space and poor conditions of the building. Conclusion: The use of the standardized WHO checklist in this first evaluation of TB IC measures in Armenia was found to be useful and feasible in identifying areas of weak IC implementation and barriers to achieving good infection control. Other TB programs may benefit from the use of this model of assessment, based on the WHO checklist.

scholarly journals PO 8527 GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY AND ASSOCIATION WITH UNCOMPLICATED MALARIA IN CONGOLESE CHILDREN CONSULTING IN A PAEDIATRIC HOSPITAL IN BRAZZAVILLE

2019 ◽  
Vol 4 (Suppl 3) ◽  
pp. A49.3-A50
Author(s):  
Nerly S Gampio Gueye ◽  
Velavan P Thirumalaisamy ◽  
Christevy Vouvoungui ◽  
Simon Ch Kobawila ◽  
David Nderu ◽  
...  
Keyword(s):  
G6pd Deficiency ◽  
Uncomplicated Malaria ◽  
Dehydrogenase Deficiency ◽  
Haemoglobin Concentration ◽  
Public Health Problem ◽  
Malaria Prevalence ◽  
Paediatric Hospital ◽  
High Prevalence ◽  
Republic Of The Congo ◽  
Glucose 6 Phosphate Dehydrogenase

BackgroundMalaria remains a public health problem in Republic of the Congo. The sub-microscopic infection including gametocytaemia constitutes a parasite reservoir that is recognised to contribute to malaria transmission. It is known that primaquine, an 8-aminoquinoline, is effective to eliminate Plasmodium falciparum (Pf) gametocytes. However, it induces haemolytic anaemia in individuals with glucose-6-phosphate dehydrogenase deficiency (G6PDd). It has been reported G6PDd also confers protection against severe malaria. To know the prevalence of G6PDd in the Congolese population is important in the case of future utilisation of this drug in the country. Therefore, in this study, we investigated 1) the prevalence of G6PDd in children infected with Pf and 2) the possible association between the presence of malaria, the presence of G6PD mutation and haemoglobin concentration.Methods229 children aged 1 to 10 years old presenting with fever (axillary T°≥37.5°C) were enrolled at the paediatric hospital Marien Ngouabi in Brazzaville. Thick and thin blood smears were done to detect and identify malaria parasites and determine parasite density. To detect the different glucose-6-phosphate dehydrogenase genotypes, a 968 bp fragment of the G6PD gene containing the polymorphisms 202G&gt1;A and 376&gt1;G was amplified by PCR followed by sequencing.ResultsMalaria prevalence was 22 (10%). With regard to G6PD analysis, it was found that 206 patients had G6PD genotype available including 74.8% (154/206) with G6PD normal, 12.1% (25/206) with heterozygous genotypes and 13.1% (27/206) with G6PD deficiency [11.6% (24/206) were male hemizygous and 1.4% (3/206) were female homozygous]. Data are further analysed to investigate the association between G6PD genotype, uncomplicated malaria, haemoglobin concentration as well as parasite densities.ConclusionA high prevalence of G6PD deficiency is reported for these Congolese children. Further investigation with larger sample size in different areas of the country is needed to design future and adapted interventions.

scholarly journals Influence of hemoglobinopathies and glucose-6-phosphate dehydrogenase deficiency on diagnosis of diabetes by HbA1c among Tanzanian adults with and without HIV: A cross-sectional study

PLoS ONE ◽  
2020 ◽  
Vol 15 (12) ◽  
pp. e0244782
Author(s):  
Belinda Kweka ◽  
Eric Lyimo ◽  
Kidola Jeremiah ◽  
Suzanne Filteau ◽  
Andrea M. Rehman ◽  
...  
Keyword(s):  
Sickle Cell ◽  
G6pd Deficiency ◽  
Sickle Cell Trait ◽  
Sub Saharan Africa ◽  
Oral Glucose ◽  
Chronic Infections ◽  
Cross Sectional ◽  
Adjusted Analysis ◽  
High Prevalence ◽  
Glucose 6 Phosphate Dehydrogenase

Introduction Hemoglobin A1c (HbA1c) is recommended for diagnosing and monitoring diabetes. However, in people with sickle cell disease (SCD), sickle cell trait (SCT), α-thalassemia or glucose-6-phosphate dehydrogenase (G6PD) deficiency, HbA1c may underestimate the prevalence of diabetes. There are no data on the extent of this problem in sub-Saharan Africa despite having high prevalence of these red blood cell disorders. Methods Blood samples from 431 adults in northwestern Tanzania, randomly selected from the prospective cohort study, Chronic Infections, Comorbidities and Diabetes in Africa (CICADA), were analysed for SCT/SCD, α-thalassemia and G6PD deficiency and tested for associations with the combined prevalence of prediabetes and diabetes (PD/DM) by HbA1c, using the HemoCue 501 HbA1c instrument, and by 2-hour oral glucose tolerance test (OGTT). Results The mean age of the participants was 40.5 (SD11.6) years; 61% were females and 71% were HIV-infected. Among 431 participants, 110 (25.5%) had SCT and none had SCD. Heterozygous α-thalassemia (heterozygous α+ AT) was present in 186 (43%) of the participants, while 52 participants (12%) had homozygous α-thalassemia (homozygous α+ AT). Furthermore, 40 (9.3%) participants, all females, had heterozygous G6PD deficiency while 24 (5.6%) males and 4 (0.9%) females had hemizygous and homozygous G6PD deficiency, respectively. In adjusted analysis, participants with SCT were 85% less likely to be diagnosed with PD/DM by HbA1c compared to those without SCT (OR = 0.15, 95% CI: 0.08, 0.26, P < 0.001). When using OGTT, in adjusted analysis, SCT was not associated with diagnosis of PD/DM while participants with homozygous α+ AT and hemizygous G6PD deficiency were more likely to be diagnosed with PD/DM. Conclusions HbA1c underestimates the prevalence of PD/DM among Tanzanian adults with SCT. Further research using other HbA1c instruments is needed to optimize HbA1c use among populations with high prevalence of hemoglobinopathies or G6PD deficiency.

FIRST AID AND TREATMENT FOR CHILDREN ACCIDENT INJURY IN BUON MA THUOT CITY, DAK LAK PROVINCE 2014

2017 ◽  
pp. 69-74
Author(s):  
Van Hung Nguyen ◽  
Van Thang Vo
Keyword(s):  
Health Care ◽  
Intervention Program ◽  
First Aid ◽  
Cross Sectional Study ◽  
Health Care Facilities ◽  
Health Staff ◽  
Cross Sectional ◽  
Care Facilities ◽  
Accident Injuries ◽  
Accident Injury

Background: Accident injuries caused has been serious heatlth problem in developing coutries. Children is vulnerable group with accident injury beucase of lacking knowlegde and exposing with risk factors in eviromental household. The treatment outcome for accident injury of children usually has more serious than other groups. The aims of this study to describle some characteristics of first aid and the outcome of treatment for children accident in Buon Ma Thuot, Dak Lak provice in 2014. Methodology: A cross-sectional study was conducted total 2,273 household which was 4,505 children aged under 16 in 8 communes, Buon Ma Thuot city, Daklak province. Interview technique with structural questionnaire and household observation methods were used for data collection. Results: The propotion of first aid was 75.9%; not received any first aid (23.8%); mortality at accident place (0.3%). At the time accident: The highest personal involving first aid was pedestrians 54.1%; 25% of health staff, self- first aid was 14.5%. Two main of first aid methods were hemostasis and bandeged with 45.5%; 28% respectiviely. After first aid, there was 80% delivering to health care facilities. The transport methods were motocycle (91.8%), car (5.6%) and ambulance (0.4%). The rate of approach health care facilities around early 6 hours were 86.7%. The characteristics of damages: sub-damages (scratches, dislocations, sprains...) were 36.9 %, deep damages (fractures, open wounds) accounted for 44.6%. Inpatient treatment was 23.9%; 91.5% medical therapy, surgery of 8.2%. The outcome of treatment were good (97.2%), sequelae/disability 2.6%. Conclusion: First aid activities for children at time and properly right were demonstrated effectively for prevented seriously outcome. There should be an intervention program for children with the appropriate models to reduce accident injuries in children; improvement first aid to communities and health care worker. Key words: accident injury, first aid, capacity first care, children under 16 years old

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