On the Physical-Chemical Effect of Colostral Antibody Binding to Escherichia Coli O 86

1975 ◽  
Vol 4 (6) ◽  
pp. 587-601 ◽  
Author(s):  
L. Edebo ◽  
F. Lindström ◽  
L. Sköldstam ◽  
O. Stendahl ◽  
C. Tagesson
Keyword(s):  
Escherichia Coli ◽  
Antibody Binding ◽  
Chemical Effect ◽  
Physical Chemical ◽  
Colostral Antibody

Structural organization of escherichia coli ribosomes as determined by immuno electron microscopy

1978 ◽  
Vol 36 (2) ◽  
pp. 166-167 ◽  
Author(s):  
G. Stöffler ◽  
R.W. Bald ◽  
J. Dieckhoff ◽  
H. Eckhard ◽  
R. Lührmann ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Ribosomal Proteins ◽  
Structural Organization ◽  
Three Dimensional ◽  
Ribosomal Subunit ◽  
Spatial Arrangement ◽  
Small Subunit ◽  
Antibody Binding ◽  
Immuno Electron Microscopy

A central step towards an understanding of the structure and function of the Escherichia coli ribosome, a large multicomponent assembly, is the elucidation of the spatial arrangement of its 54 proteins and its three rRNA molecules. The structural organization of ribosomal components has been investigated by a number of experimental approaches. Specific antibodies directed against each of the 54 ribosomal proteins of Escherichia coli have been performed to examine antibody-subunit complexes by electron microscopy. The position of the bound antibody, specific for a particular protein, can be determined; it indicates the location of the corresponding protein on the ribosomal surface.The three-dimensional distribution of each of the 21 small subunit proteins on the ribosomal surface has been determined by immuno electron microscopy: the 21 proteins have been found exposed with altogether 43 antibody binding sites. Each one of 12 proteins showed antibody binding at remote positions on the subunit surface, indicating highly extended conformations of the proteins concerned within the 30S ribosomal subunit; the remaining proteins are, however, not necessarily globular in shape (Fig. 1).

scholarly journals Publisher Correction: A novel therapeutic antibody screening method using bacterial high-content imaging reveals functional antibody binding phenotypes of Escherichia coli ST131

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Mailis Maes ◽  
Zoe A. Dyson ◽  
Sarah E. Smith ◽  
David A. Goulding ◽  
Catherine Ludden ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Screening Method ◽  
Antibody Binding ◽  
Therapeutic Antibody ◽  
High Content Imaging ◽  
Antibody Screening ◽  
Novel Therapeutic

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals Study of the changes of some physical & chemical parameters accompanied to Ovine and Caprine urinary system infections

2008 ◽  
Vol 32 (1) ◽  
pp. 198-206
Author(s):  
Wahab A. Hassan
Keyword(s):  
Escherichia Coli ◽  
Specific Gravity ◽  
Urine Sample ◽  
Urine Samples ◽  
Klebsiella Pneumonia ◽  
Urinary System ◽  
Chemical Parameters ◽  
Urine Ph ◽  
Physical Chemical

This study was devoted to determination of normal physical andchemical values of 100 sample of Ovine and Caprine urine withmicroscopically examination of urine samples sterilely collectedfrom sheep & goat slaughtered in Al-Shulla governmental slaughterhouse between may and November 2004 as well as demonstration ofchanges in these values in case of presence of microbes in urine .Thisstudy illustrated no significant differences(P<0.05) of urine analysisvalues between sheep & goat but these values significantlychanged(P<0.05)with the presence of microbes in urine and thesechanges included opacity of urine, decrease of specific gravity &increase of urine pH.25 sample were positive to protein test with concentration extent from 200to 1000mg/dl , 11 samples (44%) were positive to penzedin test. Thebacterial culture of the urine sample showed different species of bacteria thetotal presence of bacteria was 25% in both sheep & goat includingStaphylococcus aurous, Streptococcus spp, Corynobacterium renale,Escherichia coli, Protes vulgaris and Klebsiella pneumonia .

scholarly journals Groundwater quality and underground flow in southern Amazon, Brazil

2019 ◽  
Vol 10 (6) ◽  
pp. 206-217
Author(s):  
Josilena De Jesus Laureano ◽  
Caryne Ferreira Ramos ◽  
Daíse Da Silva Lopes ◽  
Lindolaine Machado de Sousa ◽  
Maria Cristina Nery do Nascimento Recktenvald ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Large Range ◽  
Domestic Sewage ◽  
Fecal Coliforms ◽  
Human Consumption ◽  
Adequate Treatment ◽  
Total Coliforms ◽  
Physical Chemical ◽  
Maximum Value ◽  
Underground Flow

The aim of this study was to evaluate the physical, chemical and microbiological groundwater parameters as well as the mapping of groundwater flow in an urban area of Presidente Médici county in Rondônia State, Southern Amazon. The water samples were collected in 11 wells in April and June of 2017, which corresponds to the dry season in the studied region. Groundwater was acidic, with a large range of nutrients concentration, low turbidity and presence of fecal coliforms in most sampled wells. The parameters that were in disagreement with Consolidation Ordinance No 05/2017/MS and CONAMA no 396/08 Resolution, were pH, turbidity, total coliforms, Escherichia coli, and nitrate. Regarding the microbiological parameter, most of the sampling sites were in disagreement with the established by the legislation that it recommends to be absent in 100mL, when it is destined for human consumption. Densities found were higher than 112,000UFC/100mL for total coliforms and higher than 56,000UFC/100mL for Escherichia coli. The pH ranged from 4.1 to 6.4, and the turbidity showed a maximum value of 6.7 uT. The nitrate concentrations were above the established level (10 mg.L-1) in April in 72.7%, and in June 50% of the wells, with a maximum of 48.5 mg.L-1. The study of the direction of the underground flow demonstrated the predominance of the flow towards the nearest river, as well as potential contributors of microbiological influences. The results indicate contamination of the groundwater by domestic sewage, being proved by the presence of coliforms and nitrate, being the water unfit for human consumption without adequate treatment.

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