Transcription of Tal-1, A Putative Oncogene Playing an Important Role in Childhood T-All, Can be Shown in Normal Peripheral Blood Cells by a Highly Sensitive Rt-Pcr Assay

1997 ◽  
Vol 14 (4) ◽  
pp. 349-358 ◽  
Author(s):  
Birgit Anderegg ◽  
Martin Horstmann ◽  
Martin Ernst ◽  
Hartmut Kabisch
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Normal Peripheral Blood ◽  
Highly Sensitive

scholarly journals Production of colony-stimulating factor by leukemic leukocytes

Blood ◽  
1976 ◽  
Vol 47 (3) ◽  
pp. 381-388 ◽  
Author(s):  
JM Goldman ◽  
KH Th'ng ◽  
D Catovsky ◽  
DA Galton
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Colony Stimulating Factor ◽  
Monocytic Leukemia ◽  
Agar Culture ◽  
Normal Peripheral Blood ◽  
Stimulating Factor ◽  
Csf Production

The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced normal or large quantities of CSF, while (with one exception) those from patients with myeloblastic leukemia produced little or no CSF. There was a general relationship between CSF production and serum lysozyme levels. Attempts to demonstrate a consistent inhibitory effect exerted by leukemic peripheral blood cells on normal leukopoiesis in vitro were negative. Results instead suggested that the addition to the feeder layer of cells from patients with monocytic leukemia could raise CSF levels above those obtained with normal peripheral blood leukocytes alone, possibly by recruiting additional CFUc from normal marrow.

scholarly journals Expression levels for many genes in human peripheral blood cells are highly sensitive to ex vivo incubation

2004 ◽  
Vol 5 (5) ◽  
pp. 347-353 ◽  
Author(s):  
E C Baechler ◽  
F M Batliwalla ◽  
G Karypis ◽  
P M Gaffney ◽  
K Moser ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Ex Vivo ◽  
Human Peripheral Blood ◽  
Peripheral Blood Cells ◽  
Expression Levels ◽  
Highly Sensitive

scholarly journals Evidence for differentiation of human leukemic blood cells in diffusion chamber culture

Blood ◽  
1977 ◽  
Vol 49 (5) ◽  
pp. 729-744 ◽  
Author(s):  
D Hoelzer ◽  
E Kurrle ◽  
H Schmucker ◽  
EB Harriss
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Diffusion Chamber ◽  
Peripheral Blood Cells ◽  
Proliferating Cells ◽  
Lymphoid Leukemia ◽  
Poor Growth ◽  
Normal Peripheral Blood ◽  
Blast Cells ◽  
Diffusion Chamber Culture

Abstract Peripheral blood cells of 21 patients with different forms of acute leukemia were cultured in diffusion chambers (5 x 10(5) cells/chamber) implanted intraperitoneally in 650 R preirradiated host mice over a period of up to 21 days. In patients with acute myeloid leukemia (AML), acute erythroleukemia (AEL), or acute myelomonocytic leukemia (AMMoL), the total number of cells which developed during this culture period exceeded the implanted value and also the values for normal peripheral blood cells from ten controls. In acute undifferentiated leukemia (AUL), two out of six patients showed considerable growth whereas the others, and also two patients with acute lymphoid leukemia (ALL), had poor growth. Differential counts revealed that the rise in total cells was due mainly to proliferation of blast cells and formation of granulopoietic cells. The latter exceeded the numbers from normal peripheral blood cells in 9 out of 13 patients with AML, AEL, or AMMoL and in 2 out of 6 patients with ALL. The production of granulopoiesis was not restricted to proliferating cells, but included mature cells which were of abnormal morphology in some cases. From the amount of granulopoiesis and the time of its development it was assumed that they were at least partly derived from leukemic blast cells. Chromosome analyses to decide whether the granulopoietic cells were of leukemic or normal cell origin are in progress.

Differential expression of wild-type and mutant NMMHC-IIA polypeptides in blood cells suggests cell-specific regulation mechanisms in MYH9 disorders

Blood ◽  
2008 ◽  
Vol 111 (6) ◽  
pp. 3015-3023 ◽  
Author(s):  
Shinji Kunishima ◽  
Motohiro Hamaguchi ◽  
Hidehiko Saito
Keyword(s):  
Differential Expression ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Inclusion Bodies ◽  
Peripheral Blood Cells ◽  
Wild Type ◽  
Normal Peripheral Blood ◽  
Peripheral Blood Cd34 ◽  
Regulation Mechanisms ◽  
Specific Regulation

Abstract MYH9 disorders such as May-Hegglin anomaly are characterized by macrothrombocytopenia and cytoplasmic granulocyte inclusion bodies that result from mutations in MYH9, the gene for nonmuscle myosin heavy chain-IIA (NMMHC-IIA). We examined the expression of mutant NMMHC-IIA polypeptide in peripheral blood cells from patients with MYH9 5770delG and 5818delG mutations. A specific antibody to mutant NMMHC-IIA (NT629) was raised against the abnormal carboxyl-terminal residues generated by 5818delG. NT629 reacted to recombinant 5818delG NMMHC-IIA but not to wild-type NMMHC-IIA, and did not recognize any cellular components of normal peripheral blood cells. Immunofluorescence and immunoblotting revealed that mutant NMMHC-IIA was present and sequestrated only in inclusion bodies within neutrophils, diffusely distributed throughout lymphocyte cytoplasm, sparsely localized on a diffuse cytoplasmic background in monocytes, and uniformly distributed at diminished levels only in large platelets. Mutant NMMHC-IIA did not translocate to lamellipodia in surface activated platelets. Wild-type NMMHC-IIA was homogeneously distributed among megakaryocytes derived from the peripheral blood CD34+ cells of patients, but coarse mutant NMMHC-IIA was heterogeneously scattered without abnormal aggregates in the cytoplasm. We show the differential expression of mutant NMMHC-IIA and postulate that cell-specific regulation mechanisms function in MYH9 disorders.

scholarly journals Production of colony-stimulating factor by leukemic leukocytes

Blood ◽  
1976 ◽  
Vol 47 (3) ◽  
pp. 381-388 ◽  
Author(s):  
JM Goldman ◽  
KH Th'ng ◽  
D Catovsky ◽  
DA Galton
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Peripheral Blood Cells ◽  
Colony Stimulating Factor ◽  
Monocytic Leukemia ◽  
Agar Culture ◽  
Normal Peripheral Blood ◽  
Stimulating Factor ◽  
Csf Production

Abstract The production of colony-stimulating factor (CSF) by the peripheral blood cells of untreated patients with acute myeloid leukemia (AML) was measured in the agar culture system using normal human bone marrow as the source of colony-forming units (CFUc). CSF production was found to be variable and was related to the morphologic subtype of AML--cells from patients with monocytic leukemia produced normal or large quantities of CSF, while (with one exception) those from patients with myeloblastic leukemia produced little or no CSF. There was a general relationship between CSF production and serum lysozyme levels. Attempts to demonstrate a consistent inhibitory effect exerted by leukemic peripheral blood cells on normal leukopoiesis in vitro were negative. Results instead suggested that the addition to the feeder layer of cells from patients with monocytic leukemia could raise CSF levels above those obtained with normal peripheral blood leukocytes alone, possibly by recruiting additional CFUc from normal marrow.

scholarly journals Evidence for differentiation of human leukemic blood cells in diffusion chamber culture

Blood ◽  
1977 ◽  
Vol 49 (5) ◽  
pp. 729-744
Author(s):  
D Hoelzer ◽  
E Kurrle ◽  
H Schmucker ◽  
EB Harriss
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Diffusion Chamber ◽  
Peripheral Blood Cells ◽  
Proliferating Cells ◽  
Lymphoid Leukemia ◽  
Poor Growth ◽  
Normal Peripheral Blood ◽  
Blast Cells ◽  
Diffusion Chamber Culture

Peripheral blood cells of 21 patients with different forms of acute leukemia were cultured in diffusion chambers (5 x 10(5) cells/chamber) implanted intraperitoneally in 650 R preirradiated host mice over a period of up to 21 days. In patients with acute myeloid leukemia (AML), acute erythroleukemia (AEL), or acute myelomonocytic leukemia (AMMoL), the total number of cells which developed during this culture period exceeded the implanted value and also the values for normal peripheral blood cells from ten controls. In acute undifferentiated leukemia (AUL), two out of six patients showed considerable growth whereas the others, and also two patients with acute lymphoid leukemia (ALL), had poor growth. Differential counts revealed that the rise in total cells was due mainly to proliferation of blast cells and formation of granulopoietic cells. The latter exceeded the numbers from normal peripheral blood cells in 9 out of 13 patients with AML, AEL, or AMMoL and in 2 out of 6 patients with ALL. The production of granulopoiesis was not restricted to proliferating cells, but included mature cells which were of abnormal morphology in some cases. From the amount of granulopoiesis and the time of its development it was assumed that they were at least partly derived from leukemic blast cells. Chromosome analyses to decide whether the granulopoietic cells were of leukemic or normal cell origin are in progress.

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