Spontaneous Thyroglobulin Autoantibody Production by Peripheral Blood Lymphocytes is Regulated by T Cells and is Augmented by Soluble T Cell Factors

Approximately 2% to 3% of circulating human T cells co-express CD4 and CD8 (CD4+, CD8+) T-cell antigens. These CD4+, CD8+ cells may be immature precursors that function to replenish functional T-cell subsets. We detected a very high level of CD4+, CD8+ cells in the peripheral blood lymphocytes of a healthy white male, ranging from 21% to 36%. The morphology of his peripheral blood lymphocytes was normal, and he has maintained an elevated level of CD4+, CD8+ cells without clinical disease over a 19-month observation period. The CD4+, CD8+ cells did not possess thymocyte membrane antigen (CD6), nor did they have increased Tac (CD25) antigen. His intact, purified blood T cells had a normal proliferative response to phytohemagglutinin and interleukin-2 (IL-2), provided help for B-cell proliferation at control levels and exposure to IL-2 resulted in generation of cytotoxic cells. However, the purified blood CD4+, CD8+ cells were deficient in these latter functions except for help in B-cell function. Despite defective function, the isolated CD4+, CD8+ cells co-expressed CD2 and CD3. Prolonged in vitro culture of CD4+, CD8+ cells was possible in the presence of recombinant IL-2. The cultured CD4+, CD8+ cells retained the double antigens (CD4 and CD8) throughout a 4-week period. It is likely these cells are less mature than CD4+, CD8- or CD4-, CD8+ T cells as the CD4+, CD8+ have less in vitro function than the former cells, but it is not yet clear if they mature into either CD4+, CD8-, or CD4-, CD8+ cells. Finally, the presence of an expanded, hypofunctioning CD4+, CD8+ cell population in a normal adult male is apparently compatible with excellent clinical health.

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Expansion of a lymphocyte population co-expressing T4 (CD4) and T8 (CD8) antigens in the peripheral blood of a normal adult male

Blood ◽

10.1182/blood.v75.10.2024.2024 ◽

1990 ◽

Vol 75 (10) ◽

pp. 2024-2029 ◽

Cited By ~ 16

Author(s):

NE Kay ◽

N Bone ◽

M Hupke ◽

AP Dalmasso

Keyword(s):

T Cells ◽

T Cell ◽

B Cell ◽

Adult Male ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Normal Adult ◽

Cd8 Cells ◽

Blood Lymphocytes

Abstract Approximately 2% to 3% of circulating human T cells co-express CD4 and CD8 (CD4+, CD8+) T-cell antigens. These CD4+, CD8+ cells may be immature precursors that function to replenish functional T-cell subsets. We detected a very high level of CD4+, CD8+ cells in the peripheral blood lymphocytes of a healthy white male, ranging from 21% to 36%. The morphology of his peripheral blood lymphocytes was normal, and he has maintained an elevated level of CD4+, CD8+ cells without clinical disease over a 19-month observation period. The CD4+, CD8+ cells did not possess thymocyte membrane antigen (CD6), nor did they have increased Tac (CD25) antigen. His intact, purified blood T cells had a normal proliferative response to phytohemagglutinin and interleukin-2 (IL-2), provided help for B-cell proliferation at control levels and exposure to IL-2 resulted in generation of cytotoxic cells. However, the purified blood CD4+, CD8+ cells were deficient in these latter functions except for help in B-cell function. Despite defective function, the isolated CD4+, CD8+ cells co-expressed CD2 and CD3. Prolonged in vitro culture of CD4+, CD8+ cells was possible in the presence of recombinant IL-2. The cultured CD4+, CD8+ cells retained the double antigens (CD4 and CD8) throughout a 4-week period. It is likely these cells are less mature than CD4+, CD8- or CD4-, CD8+ T cells as the CD4+, CD8+ have less in vitro function than the former cells, but it is not yet clear if they mature into either CD4+, CD8-, or CD4-, CD8+ cells. Finally, the presence of an expanded, hypofunctioning CD4+, CD8+ cell population in a normal adult male is apparently compatible with excellent clinical health.

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T cell receptor Vb5 and Vb17 clonal diversity in cerebrospinal fluid and peripheral blood lymphocytes of multiple sclerosis patients

Multiple Sclerosis Journal ◽

10.1177/135245859800400313 ◽

1998 ◽

Vol 4 (3) ◽

pp. 154-161 ◽

Cited By ~ 4

Author(s):

P Lozeron ◽

D Chabas ◽

B Duprey ◽

O Lyon-Caen ◽

R Liblau

Keyword(s):

Central Nervous System ◽

T Cells ◽

Nervous System ◽

T Cell ◽

T Cell Receptor ◽

Peripheral Blood ◽

Cell Receptor ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Multiple Sclerosis Patients

To better characterize the cellular immune response taking place in the MS central nervous system, we investigated the blood and CSF T cell receptor (TCR) Vβ5 and Vb17 repertoire in HLA-typed patients with recently diagnosed MS or other neurological diseases (OND). Using a RT-PCR based technique, we analysed directly ex vivo the CDR3 size of TCR β chains utilizing Vβ5 (eight patients with MS and one with OND) or Vβ17 (eight patients with MS and six with OND) gene segments on paired blood-CSF samples. Globally, the analysis of Vβ5-Jβ and Vβ17-Jβ repertoire showed a less diverse pattern in the CSF samples than in the corresponding peripheral blood lymphocytes both in MS and in OND patients. However, we did not detect any recurrent clonal expansion within the Vb5+ T cells in MS patients, underlining the potential limits of Vβ5- based immunotherapy in MS. We found an expanded T cell population using the same Vβ17-Jβ1.6 combination with identical CDR3 length in the CSF of three MS patients and none of the control patients. These results suggest selective expansion of T cells expressing this segment gene in the MS central nervous system.

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Human T Cell Priming Assay: Depletion of Peripheral Blood Lymphocytes in CD25+ Cells Improves the In Vitro Detection of Weak Allergen-Specific T Cells

T Lymphocytes as Tools in Diagnostics and Immunotoxicology - Experientia Supplementum ◽

10.1007/978-3-0348-0726-5_7 ◽

2013 ◽

pp. 89-100 ◽

Cited By ~ 2

Author(s):

Marc Vocanson ◽

Amine Achachi ◽

Virginie Mutez ◽

Magalie Cluzel-Tailhardat ◽

Béatrice Le Varlet ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes ◽

Human T Cell ◽

T Cell Priming

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Peripheral blood lymphocytes and monocytes heterogeneity in SLE patients treated by Belimumab

10.21203/rs.3.rs-622554/v1 ◽

2021 ◽

Author(s):

Jinghan Yang ◽

Shushan Yan ◽

Haibo Li ◽

Chunjuan Yang ◽

Lili Zhang ◽

...

Keyword(s):

T Cells ◽

T Cell ◽

B Cells ◽

Nk Cells ◽

Lupus Erythematosus ◽

Peripheral Blood ◽

Peripheral Blood Lymphocytes ◽

Systemic Lupus ◽

Blood Lymphocytes ◽

Hla Dr

Abstract Objective: To estimate the effect of Belimumab on the heterogeneity of peripheral blood lymphocytes and monocytes subsets in systemic lupus erythematosus (SLE).Methods: There were three groups of populations, namely health controls (HC, n = 92), SLE patients treated (n = 26) and un-treated with BAFF (n = 155) in the present study. Cell subsets of CD3+T cells, CD3+CD4+T cells, CD3+CD8+T cells, CD19+B cells, CD16+CD56+NK cells, CD14+HLA-DR+monocytes, CD14+CD206+monocytes and CD14+CD163+monocytes were estimated by flow cytometry. Results: Compared with HC group, SLE patients had a higher level of CD3+T cells and CD3+CD8+T cells, but a lower level of CD3+CD4+T cells, CD16+CD56+NK cells, CD14+CD206+monocytes, and CD14+CD163+monocytes. Besides, the ratio of CD3+CD4+/CD3+CD8+T cells was much lower in SLE patients. Belimumab could effectively decrease CD19+B cells but increase CD3+T cell and CD3+CD8+T cells in the peripheral blood of SLE patients. Moreover, SLE patients had decreased CD14+CD206+ monocytes and CD14+CD163+ monocytes in peripheral blood, while Belimumab therapy did not affect the heterogeneity of monocytes in SLE.Conclusions: Our results revealed the heterogeneity of lymphocytes and monocytes in SLE patients. The clinical efficacy of Belimumab in SLE treatment is remarkable due to its significant effect on down-regulating B cell but up-regulating CD3+T cell and CD3+CD8+T cells in SLE.

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