Sanggenon C and O inhibit NO production, iNOS expression and NF-κB activation in LPS-induced RAW264.7 cells

2011 ◽  
Vol 34 (1) ◽  
pp. 84-88 ◽  
Author(s):  
Nguyen Tien Dat ◽  
Phung Thi Xuan Binh ◽  
Le Thi Phuong Quynh ◽  
Hoang Thanh Huong ◽  
Chau Van Minh
Keyword(s):  
Inos Expression ◽  
Raw264.7 Cells ◽  
No Production

scholarly journals Anti-Inflammatory Effects of Catalpalactone Isolated from Catalpa ovata in LPS-Induced RAW264.7 Cells

Molecules ◽  
2019 ◽  
Vol 24 (7) ◽  
pp. 1236 ◽  
Author(s):  
Hyo-Young Kim ◽  
Ah-Reum Han ◽  
Yun-Seo Kil ◽  
Eun Seo ◽  
Chang Jin
Keyword(s):  
Nuclear Factor Κb ◽  
Inos Expression ◽  
Raw264.7 Cells ◽  
No Production ◽  
Signal Transducer ◽  
Interferon Β ◽  
Catalpa Ovata ◽  
Anti Inflammatory ◽  
Factor Α ◽  
Necrosis Factor

Catalpa ovata (Bignoniaceae) is widely distributed throughout Korea, China, and Japan. This study investigated the anti-inflammatory effects of catalpalactone isolated from C. ovata in lipopolysaccharide (LPS)-induced RAW264.7 cells. Catalpalactone significantly inhibited nitric oxide (NO) production and inducible NO synthase (iNOS) expression in LPS-induced RAW264.7 cells. The levels of cytokines such as interleukin-6 and tumor necrosis factor-α were reduced under catalpalactone exposure in LPS-induced RAW264.7 cells. Additionally, catalpalactone suppressed signal transducer and activator of transcription 1 (STAT-1) protein expression and interferon-β (IFN-β) production. Treatment with catalpalactone prevented interferon regulatory factor 3 (IRF3) and nuclear factor-κB (NF-κB) activation. Taken together, these results suggest that the anti-inflammatory effects of catalpalactone are associated with the suppression of NO production and iNOS expression through the inhibition of IRF3, NF-κB, and IFN-β/STAT-1 activation.

Inhibitory effect of citral on NO production by suppression of iNOS expression and NF-κB activation in RAW264.7 cells

2008 ◽  
Vol 31 (3) ◽  
pp. 342-349 ◽  
Author(s):  
Hwa Jeong Lee ◽  
Heon Sang Jeong ◽  
Dae Joong Kim ◽  
Young Hee Noh ◽  
Dong Yeon Yuk ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Inos Expression ◽  
Raw264.7 Cells ◽  
No Production

Polyphenols from Cymbopogon citratus inhibit iNOS expression and NO production – a promising source of new anti-inflammatory drugs

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
V Francisco ◽  
A Figueirinha ◽  
B Neves ◽  
C Garcia-Rodriguez ◽  
M Lopes ◽  
...  
Keyword(s):  
Inos Expression ◽  
No Production ◽  
Cymbopogon Citratus ◽  
Inflammatory Drugs ◽  
Anti Inflammatory ◽  
Promising Source

scholarly journals New Truxinic and Truxillic Acid Sucrose Diesters From the Leaves of Trigonostemon honbaensis

2021 ◽  
Vol 16 (2) ◽  
pp. 1934578X2199914
Author(s):  
Ninh Khac Ban ◽  
Bui Huu Tai ◽  
Vu Kim Thu ◽  
Phan Van Kiem
Keyword(s):  
Nmr Spectra ◽  
Raw264.7 Cells ◽  
No Production ◽  
Inhibitory Effects ◽  
Esi Ms ◽  
Chemical Structures ◽  
Extensive Analysis ◽  
Truxillic Acid

A new δ-truxinic acid sucrose diester and a new ε-truxillic acid sucrose diester (named trigohonbanosides E and F) were isolated from the leaves of Trigonostemon honbaensis. Their chemical structures were determined by extensive analysis of their HR-ESI-MS and NMR spectra. At a concentration of 20 µM, trigohonbanosides E and F exhibited weak inhibitory effects on NO production in LPS-activated RAW264.7 cells with inhibitory percentages of 22.7% ± 1.1% and 18.5% ± 1.4%, respectively.

Nitric oxide-mediated regulation of transepithelial sodium and chloride transport in murine nasal epithelium

1999 ◽  
Vol 276 (3) ◽  
pp. L466-L473 ◽  
Author(s):  
Heather L. Elmer ◽  
Kristine G. Brady ◽  
Mitchell L. Drumm ◽  
Thomas J. Kelley
Keyword(s):  
Nitric Oxide ◽  
Chloride Transport ◽  
Chloride Secretion ◽  
Inos Expression ◽  
Potential Difference ◽  
Nasal Epithelium ◽  
Sodium Absorption ◽  
No Production ◽  
Transepithelial Potential ◽  
Transepithelial Potential Difference

Transepithelial ion transport is regulated by a variety of cellular factors. In light of recent evidence that nitric oxide (NO) production is decreased in cystic fibrosis airways, we examined the role of NO in regulating sodium and chloride transport in murine nasal epithelium. Acute intervention with the inducible NO synthase (iNOS)-selective inhibitor S-methylisothiourea resulted in an increase of amiloride-sensitive sodium absorption observed as a hyperpolarization of nasal transepithelial potential difference. Inhibition of iNOS expression with dexamethasone also hyperpolarized transepithelial potential difference, but only a portion of this increase proved to be amiloride sensitive. Chloride secretion was significantly inhibited in C57BL/6J mice by the addition of both S-methylisothiourea and dexamethasone. Mice lacking iNOS expression [NOS2(−/−)] also had a decreased chloride-secretory response compared with control mice. These data suggest that constitutive NO production likely plays some role in the downregulation of sodium absorption and leads to an increase in transepithelial chloride secretion.

Induction of inducible nitric oxide synthase increases the production of reactive oxygen species in RAW264.7 macrophages

2010 ◽  
Vol 30 (4) ◽  
pp. 233-241 ◽  
Author(s):  
Kai Zhao ◽  
Zhen Huang ◽  
Hongling Lu ◽  
Juefei Zhou ◽  
Taotao Wei
Keyword(s):  
Nitric Oxide ◽  
Reactive Oxygen Species ◽  
Nitric Oxide Synthase ◽  
Reactive Oxygen ◽  
Inos Expression ◽  
Raw264.7 Cells ◽  
Ros Production ◽  
Oxygen Species ◽  
Raw264.7 Macrophages ◽  
Oxide Synthase

Macrophages produce a large volume of ROS (reactive oxygen species) through respiratory burst. However, the influence of iNOS [inducible NOS (nitric oxide synthase)] activation on ROS production remains unclear. In the present study, the kinetic generation of ROS in RAW264.7 murine macrophages was monitored by chemiluminescence. PMA induces a robust chemiluminescence in RAW264.7 cells, suggesting PKC (protein kinase C)-related assembly and activation of NOX (NADPH oxidase). The effects of iNOS induction on ROS production were examined. Induction of iNOS expression in RAW264.7 cells with LPS (lipopolysaccharide; 1 μg/ml) causes a significant increase in PMA-induced chemiluminescence, which could be enhanced by the NOS substrate, L-arginine, and could be abolished by the NOS inhibitor, L-NNA (NG-nitro-L-arginine). Further experiments reveal that induction of iNOS expression enhances the PMA-stimulated phosphorylation of the p47phox subunit of NOX, and promotes the relocalization of cytosolic p47phox and p67phox subunits to the membrane. Inhibition of PKCζ by its myristoylated pseudosubstrate significantly decreased the PMA-stimulated phosphorylation of the p47phox in LPS-pretreated cells, suggesting that PKCζ is involved in the iNOS-dependent assembly and activation of NOX. Taken together, the present study suggests that the induction of iNOS upregulates the PMA-induced assembly of NOX and leads to the enhanced production of ROS via a PKCζ-dependent mechanism.

scholarly journals U-Bang-Haequi Tang: A Herbal Prescription that Prevents Acute Inflammation through Inhibition of NF-κB-Mediated Inducible Nitric Oxide Synthase

2014 ◽  
Vol 2014 ◽  
pp. 1-12 ◽  
Author(s):  
Min Hwangbo ◽  
Ji Yun Jung ◽  
Sung Hwan Ki ◽  
Sang Mi Park ◽  
Kyung Hwan Jegal ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Acute Inflammation ◽  
Nuclear Translocation ◽  
Inflammatory Cells ◽  
Raw264.7 Cells ◽  
No Production ◽  
Traditional Korean Medicine ◽  
Korean Medicine ◽  
Forsythia Suspensa ◽  
Herbal Prescription

Since antiquity, medical herbs have been prescribed for both treatment and preventative purposes. Herbal formulas are used to reduce toxicity as well as increase efficacy in traditional Korean medicine.U-bang-haequi tang(UBT) is a herbal prescription containingArctii fructusandForsythia suspensaas its main components and has treated many human diseases in traditional Korean medicine. This research investigated the effects of UBT against an acute phase of inflammation. For this, we measured induction of nitric oxide (NO) and related proteins in macrophage cell line stimulated by lipopolysaccharide (LPS). Further, paw swelling was measured in carrageenan-treated rats. Carrageenan significantly induced activation of inflammatory cells and increases in paw volume, whereas oral administration of 0.3 or 1 g/kg/day of UBT inhibited the acute inflammatory response. In RAW264.7 cells, UBT inhibited mRNA and protein expression levels of iNOS. UBT treatment also blocked elevation of NO production, nuclear translocation of NF-κB, phosphorylation of Iκ-Bαinduced by LPS. Moreover, UBT treatment significantly blocked the phosphorylation of p38 and c-Jun NH2-terminal kinases by LPS. In conclusion, UBT prevented both acute inflammation in rats as well as LPS-induced NO and iNOS gene expression through inhibition of NF-κB in RAW264.7 cells.

Arctigenin inhibits lipopolysaccharide-induced iNOS expression in RAW264.7 cells through suppressing JAK-STAT signal pathway

2011 ◽  
Vol 11 (8) ◽  
pp. 1095-1102 ◽  
Author(s):  
Xianjuan Kou ◽  
Shimei Qi ◽  
Wuxing Dai ◽  
Lan Luo ◽  
Zhimin Yin
Keyword(s):  
Signal Pathway ◽  
Inos Expression ◽  
Raw264.7 Cells

scholarly journals Effects of Lycopene, Indole-3-Carbinol, and Luteolin on Nitric Oxide Production and iNOS Expression are Organ-Specific in Rats

2010 ◽  
Vol 61 (3) ◽  
pp. 275-285 ◽  
Author(s):  
Evita Rostoka ◽  
Sergejs Isajevs ◽  
Larisa Baumane ◽  
Aija Line ◽  
Karina Silina ◽  
...  
Keyword(s):  
Gene Expression ◽  
Brain Cortex ◽  
Natural Compounds ◽  
Inos Expression ◽  
No Production ◽  
Nitric Oxide Production ◽  
Inos Gene ◽  
Organ Specific ◽  
The Brain ◽  
Inos Gene Expression

Effects of Lycopene, Indole-3-Carbinol, and Luteolin on Nitric Oxide Production and iNOS Expression are Organ-Specific in RatsNatural compounds are known to modify NO content in tissues; however, the biological activity of polyphenol-rich food often does not correspond to the effects of individual polyphenols on NO synthase activity. The aim of this study was to see how natural compounds luteolin, indole-3-carbinol, and lycopene modify NO production in rat tissues and change the expression of the iNOS gene and protein. Indole-3-carbinol produced multiple effects on the NO level; it significantly decreased NO concentration in blood, lungs, and skeletal muscles and increased it in the liver. Indole-3-carbinol enhanced lipopolyssaccharide (LPS)-induced NO production in all rat organs. It decreased iNOS gene expression in the brain cortex of animals that did not receive LPS and up-regulated it in the LPS-treated animals. Lycopene increased the iNOS gene transcription rate in the brain cortex of LPS-treated animals. Luteolin did not modify NO production in any organ of LPS-untreated rats, nor did it affect gene expression in the liver. In the brain it slightly decreased iNOS gene expression. Luteolin decreased NO production in the blood of LPS-treated animals and the number of iNOS-positive cells in these animals. Our results suggest that changes in tissue NO levels caused by natural compounds cannot be predicted from their effect on NOS expression or activity obtained in model systems. This stresses the importance of direct measurements of NO and NOS expression in animal tissues.

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