Zebrafish thrombocyte aggregation by whole blood aggregometry and flow cytometry

Platelets ◽  
2015 ◽  
Vol 26 (7) ◽  
pp. 613-619 ◽  
Author(s):  
Hemalatha Sundaramoorthi ◽  
Rekha Panapakam ◽  
Pudur Jagadeeswaran
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Thrombocyte Aggregation ◽  
Whole Blood Aggregometry

A Simple and Rapid Method to Determine GPIIb/IIIa-Receptor Inhibitor Activity in Whole Blood

1999 ◽  
Vol 19 (03) ◽  
pp. 134-138
Author(s):  
Gitta Kühnel ◽  
A. C. Matzdorff
Keyword(s):  
Flow Cytometry ◽  
Platelet Count ◽  
Blood Sample ◽  
Platelet Activation ◽  
Whole Blood ◽  
Receptor Occupancy ◽  
Aggregate Formation ◽  
Inhibitor Activity ◽  
Receptor Inhibitor

SummaryWe studied the effect of GPIIb/IIIa-inhibitors on platelet activation with flow cytometry in vitro. Citrated whole blood was incubated with increasing concentrations of three different GPIIb/IIIa-inhibitors (c7E3, DMP728, XJ757), then thrombin or ADP were added and after 1 min the sample was fixed. Samples without c7E3 but with 0.1 U/ml thrombin had a decrease in platelet count. Samples with increasing concentrations of c7E3 had a lesser or no decrease in platelet count. The two other inhibitors (DMP 725, XJ757) gave similar results. GPIIb/IIIa-inhibitors prevent aggregate formation and more single platelets remain in the blood sample. The agonist-induced decrease in platelet count correlates closely with the concentration of the GPIIb/IIIa inhibitor and receptor occupancy. This correlation may be used as a simple measure for inhibitor activity in whole blood.

scholarly journals An easy and reliable whole blood freezing method for flow cytometry immuno‐phenotyping and functional analyses

2021 ◽  
Author(s):  
Cecile Braudeau ◽  
Nina Salabert‐Le Guen ◽  
Justine Chevreuil ◽  
Marie Rimbert ◽  
Jerome C. Martin ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Whole Blood ◽  
Freezing Method ◽  
Functional Analyses

The Effects of Heparin, Protamine, and Heparinase 1 on Platelets in vitro Using Whole Blood Flow Cytometry

2000 ◽  
Vol 9 (4) ◽  
pp. 808-812 ◽  
Author(s):  
Sibylle A. Kozek-Langenecker ◽  
S. Fazal Mohammad ◽  
Takahisa Masaki ◽  
Craig Kamerath ◽  
Alfred K. Cheung
Keyword(s):  
Flow Cytometry ◽  
Blood Flow ◽  
Whole Blood
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