Granulocyte-Colony Stimulating Factor, Granulocyte-Macrophage Colony Stimulating Factor, PIXY-321, Stem Cell Factor, Interleukin-3, and Interleukin-7: Receptor Binding and Effects on Clonogenic Proliferation in Acute Lymphoblastic Leukemia

1994 ◽  
Vol 16 (1-2) ◽  
pp. 79-88 ◽  
Author(s):  
Doris Drach ◽  
Zeev Estrov ◽  
Shourong Zhao ◽  
Johannes Drach ◽  
Anne Cork ◽  
...  
Keyword(s):  
Stem Cell ◽  
Acute Lymphoblastic Leukemia ◽  
Stem Cell Factor ◽  
Lymphoblastic Leukemia ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Interleukin 7 ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

Granulocyte—Macrophage Colony—Stimulating Factor in Newly Diagnosed Adult Patients with Acute Lymphoblastic Leukemia Treated with Conventional Chemotherapy

Hematology ◽  
1996 ◽  
Vol 1 (3) ◽  
pp. 207-214
Author(s):  
Mohammed S. Harakati ◽  
Abdulkareem Al-Momen ◽  
Dahish S. Ajarim ◽  
Fahad I. Al-Mohareb ◽  
Abdulrahman Al Diab ◽  
...  
Keyword(s):  
Acute Lymphoblastic Leukemia ◽  
Lymphoblastic Leukemia ◽  
Adult Patients ◽  
Newly Diagnosed ◽  
Colony Stimulating Factor ◽  
Conventional Chemotherapy ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

scholarly journals Recombinant human stem cell factor enhances the formation of colonies by CD34+ and CD34+lin- cells, and the generation of colony-forming cell progeny from CD34+lin- cells cultured with interleukin-3, granulocyte colony-stimulating factor, or granulocyte-macrophage colony-stimulating factor

Blood ◽  
1991 ◽  
Vol 77 (11) ◽  
pp. 2316-2321 ◽  
Author(s):  
ID Bernstein ◽  
RG Andrews ◽  
KM Zsebo
Keyword(s):  
Stem Cell Factor ◽  
Liquid Culture ◽  
Granulocyte Colony Stimulating Factor ◽  
Human Stem Cell ◽  
Colony Stimulating Factor ◽  
Gm Csf ◽  
Cd34 Cells ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Stimulating Factor

We tested the ability of recombinant human stem cell factor (SCF) to stimulate isolated marrow precursor cells to form colonies in semisolid media and to generate colony-forming cells (CFC) in liquid culture. SCF, in combination with interleukin-3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), or granulocyte colony-stimulating factor (G-CSF) caused CD34+ cells to form increased numbers of granulocyte-macrophage colonies (CFU-GM), and to form macroscopic erythroid burst-forming units (BFU-E) in the presence of IL-3, erythropoietin (Epo), and SCF. We tested isolated CD34+lin- cells, a minor subset of CD34+ cells that did not display antigens associated with lymphoid or myeloid lineages, and CD34+lin+ cells, which contain the vast majority of CFC, and found that the enhanced colony growth was most dramatic within the CD34+lin- population. CD34+lin- cells cultured in liquid medium containing SCF combined with IL-3, GM-CSF, or G-CSF gave rise to increased numbers of CFC. Maximal numbers of CFU-GM were generated from CD34+lin- cells after 7 to 21 days of culture, and required the presence of SCF from the initiation of liquid culture. The addition of SCF to IL-3 and/or G-CSF in cultures of single CD34+lin- cells resulted in increased numbers of CFC due to the proliferation of otherwise quiescent precursors and an increase in the numbers of CFC generated from individual precursors. These studies demonstrate the potent synergistic interaction between SCF and other hematopoietic growth factors on a highly immature population of CD34+lin- precursor cells.

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