Ontogeny of amino-acid metabolism-enzymes in peripheral tissues of developing rats

The phenylalanine/tyrosine degradation pathway is frequently described as a catabolic pathway that funnels aromatic amino acids into citric acid cycle intermediates. Previously, we demonstrated that the accumulation of tyrosine generated during the hydrolysis of blood meal proteins in Rhodnius prolixus is potentially toxic, a harmful outcome that is prevented by the action of the first two enzymes in the tyrosine degradation pathway. In this work, we further evaluated the relevance of all other enzymes involved in phenylalanine/tyrosine metabolism in the physiology of this insect. The knockdown of most of these enzymes produced a wide spectrum of distinct phenotypes associated with reproduction, development and nymph survival, demonstrating a highly pleiotropic role of tyrosine metabolism. The phenotypes obtained for two of these enzymes, homogentisate dioxygenase and fumarylacetoacetase, have never before been described in any arthropod. To our knowledge, this report is the first comprehensive gene-silencing analysis of an amino acid metabolism pathway in insects. Amino acid metabolism is exceptionally important in haematophagous arthropods due to their particular feeding behaviour.

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Influence of diet and non-essential nitrogen on amino acid metabolism enzymes of developing rats

Nutrition Research ◽

10.1016/s0271-5317(05)80580-9 ◽

1992 ◽

Vol 12 (8) ◽

pp. 955-963

Author(s):

M.J. Salvadó ◽

Ll. Arola

Keyword(s):

Amino Acid ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Metabolism Enzymes

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Effect of diet and essential amino acids gavage on young rat amino acid metabolism enzymes

Comparative Biochemistry and Physiology Part A Physiology ◽

10.1016/0300-9629(92)90186-t ◽

1992 ◽

Vol 103 (4) ◽

pp. 817-822

Author(s):

J. Salvadó ◽

Ll. Arola

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Essential Amino Acids ◽

Metabolism Enzymes ◽

Young Rat

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Widespread Inter- and Intra-Domain Horizontal Gene Transfer of d-Amino Acid Metabolism Enzymes in Eukaryotes

Frontiers in Microbiology ◽

10.3389/fmicb.2016.02001 ◽

2016 ◽

Vol 7 ◽

Cited By ~ 8

Author(s):

Miguel A. Naranjo-Ortíz ◽

Matthias Brock ◽

Sascha Brunke ◽

Bernhard Hube ◽

Marina Marcet-Houben ◽

...

Keyword(s):

Amino Acid ◽

Gene Transfer ◽

Horizontal Gene Transfer ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Metabolism Enzymes

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THE ACTIVITY OF ENZYMES OF AMINO ACID METABOLISM OF THE PLACENTA IN DIFFERENT TERMS OF THE PHYSIOLOGICAL AND COMPLICATED PREGNANCY

Russian Clinical Laboratory Diagnostics ◽

10.18821/0869-2084-2019-64-5-260-264 ◽

2019 ◽

Vol 64 (5) ◽

pp. 260-264

Author(s):

Tatyana Nikolaevna Pogorelova ◽

V. O. Gunko ◽

A. A. Mikhelson ◽

A. A. Nikashina ◽

A. F. Mikhelson ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Amino Acid Metabolism ◽

Acid Metabolism ◽

Amino Acid Content ◽

Exchange Chromatography ◽

Glutathione Synthetase ◽

Spectrophotometric Methods ◽

Metabolism Enzymes ◽

Complicated Pregnancy

The activity of amino acid metabolism enzymes and the content of free amino acids in the placenta during physiological pregnancy and placental insufficiency (PI) were studied using spectrophotometric methods and ion-exchange chromatography. It was found that in PI placental activity of the studied enzymes: alanine-, cysteine-e, tyrosine-, glutamino- transferase, glutathione synthetase, glutamate dehydrogenase decreases at different periods of gestation. The opposite variations occur for aspartataminotranferase and glutaminase. Similar changes are detected for amino acids synthesized or used in the course of appropriate reactions: aspartic acid, glutamic acid, glutamine, alanine, cysteine, tyrosine, arginine. The correlation between enzyme activity and amino acid content was revealed. Different periods of pregnancy are characterized by varying degrees of change, especially expressed in the second trimester, characterized by the most intense growth and development of the fetus, and its increased needs for trophic material. The revealed changes obviously play a pathogenetic role in the formation and further development of PI.

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