scholarly journals Characterisation of the S1 satellite DNA family in Rana dalmatina

2021 ◽  
Author(s):  
Vito Abbruzzese
Keyword(s):  
Satellite Dna ◽  
Genomic Dna ◽  
Common Ancestor ◽  
Repeated Dna ◽  
Major Satellite ◽  
Different Types ◽  
Tandem Structure ◽  
High Sequence Homology ◽  
The Moment ◽  
Rana Dalmatina

In this study, the DNA of Rana dalmatina was digested with Asp 718I and the two bands of highly repeated DNA produced were cloned and characterised. The largest fragment (494 bp) corresponded to the entire repetitive unit of the major satellite DNA (RdS1a), while the smaller fragment of 385 bp corresponded to the major fragment of RdS1a produced by digestion. A fragment of 332 bpbcorresponding to the repetitive unit of satellite S1b (RdS1b) was instead achieved by digestion with Eco RV. RdS1b is highly homologous to the corresponding portion of the repetition of RdS1a and presents the first 36 bp repeated and inverted. This suggested that RdS1b would have been derived from satellite S1a by two distinct and subsequent events. Further, the high sequence homology and length between RdS1a and the S1a of Rana italica (RiS1a) confirmed the hypothesis that the satellite S1a is antecedent to S1b and inherited from a common ancestor. Southern blots of R. dalmatina genomic DNA digested with Asp 718I produced hybrid bands of fragments of different sizes containing in addition to the satellite S1a, also one or more copies of the S1b satellites. The only sequenced band at the moment corresponded to the repetitive unit of the satellite RdS1a + b (826 bp) deleted of the fragment Asp 718I less than RdS1a (109 bp), while the other double bands should almost certainly correspond to repetitive units of satellites RdS1a + 2b and RdS1a + 3b. Our data suggested different satellite DNA organisation in R. dalmatina, including the tandem structure of the repetitive units of the RdS1a or RdS1b. Our data also suggested the existence in R. dalmatina of at least four different types of hybrid repeating units in all the populations examined.

scholarly journals Branching processes with interactions: subcritical cooperative regime

2021 ◽  
Vol 53 (1) ◽  
pp. 251-278
Author(s):  
Adrián González Casanova ◽  
Juan Carlos Pardo ◽  
José Luis Pérez
Keyword(s):  
Population Genetics ◽  
Branching Processes ◽  
Jump Diffusion ◽  
Pairwise Interaction ◽  
Pairwise Interactions ◽  
Different Types ◽  
Interesting Object ◽  
The Moment ◽  
Dynamics Of Populations

AbstractIn this paper, we introduce a family of processes with values on the nonnegative integers that describes the dynamics of populations where individuals are allowed to have different types of interactions. The types of interactions that we consider include pairwise interactions, such as competition, annihilation, and cooperation; and interactions among several individuals that can be viewed as catastrophes. We call such families of processes branching processes with interactions. Our aim is to study their long-term behaviour under a specific regime of the pairwise interaction parameters that we introduce as the subcritical cooperative regime. Under such a regime, we prove that a process in this class comes down from infinity and has a moment dual which turns out to be a jump-diffusion that can be thought as the evolution of the frequency of a trait or phenotype, and whose parameters have a classical interpretation in terms of population genetics. The moment dual is an important tool for characterizing the stationary distribution of branching processes with interactions whenever such a distribution exists; it is also an interesting object in its own right.

scholarly journals Identifikasi Ekstraksi Fitur untuk Gerakan Tangan dalam Bahasa Isyarat (SIBI) Menggunakan Sensor MYO Armband

2019 ◽  
Vol 19 (1) ◽  
pp. 127-137
Author(s):  
Angga Rahagiyanto
Keyword(s):  
Sign Language ◽  
Cross Validation ◽  
Hand Movements ◽  
Raw Data ◽  
Moment Invariant ◽  
Recognition Process ◽  
Movement Recognition ◽  
Myo Armband ◽  
Different Types ◽  
The Moment

Indonesian: Indonesian SIBI has been widely reviewed by researchers using different types of cameras and sensors. The ultimate goal is to produce a strong, fast and accurate movement recognition process. One that supports talk of movement using sensors on the MYO Armband tool. This paper explains how to use raw data generated from the MYO Armband sensor and extract integration so that it can be used to facilitate complete hand, arm and combination movements in the SIBI sign language dictionary. MYO armband uses five sensors: accelerometer, gyroscope, orientation, euler-orientation and EMG. Each sensor produces data that is different in scale and size. This requires a process to make the data uniform. This study uses the min-max method to normalize any data on the MYO Armband sensor and the Moment Invariant method to extract the vector features of hand movements. Testing is done using sign language Movement statistics both dynamic signals. Testing is done using cross validation.

THE SPECIFIC OF HIERARCHICAL COLLISIONS RESOLUTION IN RUSSIAN LAW

2021 ◽  
pp. 34-37
Author(s):  
Наталья Алексеевна Макарова
Keyword(s):  
Law Enforcement ◽  
Special Property ◽  
Basic Rule ◽  
Constitutional State ◽  
Different Types ◽  
Russian Law ◽  
The Moment ◽  
Practical Recommendations ◽  
The Way ◽  
Legal Force

Статья посвящена рассмотрению особенностей и формулированию практических рекомендаций по разрешению иерархических юридических коллизий в российском праве. Автор статьи предлагает трактовать иерархическую (субординационную) юридическую коллизию расширительно: не только как противоречие между двумя или более нормативно-правовыми актами, но и как возможное противоречие между правовыми актами разных типов, включая правоприменительные (индивидуальные) и интерпретационные акты. Подчеркивается, что базовым правилом разрешения любой иерархической юридической коллизии должно быть правило юридической силы. В то же время в настоящий момент в российском праве нормы, определяющие данное понятие, а равно устанавливающие четкие механизмы преодоления юридических коллизий, включая иерархические, отсутствуют. Под юридической силой предлагается понимать особое свойство правовых актов, благодаря наличию которого данные акты формируют иерархию, и нижестоящие акты вышестоящим противоречить не могут. Выдвигается тезис о том, что не типичные иерархические коллизии в праве (коллизии между нормативно-правовым актом, с одной стороны, и правоприменительным или интерпретационным актом, с другой, а равно несколькими правоприменительными и (или) интерпретационными актами между собой) как разновидность иерархической юридической коллизии должны разрешаться с обязательным использованием правила юридической силы. То есть акты нормативного толкования и правоприменения должны быть приравнены по юридической силе к интерпретируемым и применяемым актам. Именно такой путь позволит избежать остановок в работе механизма реализации права, которых, как известно, современное правовое государство позволить себе не может. The article is devoted to the consideration of the features and the development of practical recommendations for resolving hierarchical legal collisions in Russian law. The author of the article proposes to interpret the hierarchical legal collision broadly, not only as a contradiction between regulatory legal acts, but also as a possible contradiction between legal acts of different types. It is emphasized that the basic rule for resolving any hierarchical legal collision should be the rule of legal force. However, at the moment in Russian law there are no norms defining this concept, as well as establishing clear mechanisms for overcoming legal conflicts, including hierarchical ones. It is proposed to understand legal force as a special property of legal acts, due to the presence of which these acts form a hierarchy, and lower-level acts cannot contradict higher-level ones. The thesis is put forward that non-typical hierarchical collisions in law should also be resolved with the obligatory use of the rule of legal force. This means that the acts of normative interpretation and law enforcement should be equated in legal force with the interpreted and applied acts. This is the way to avoid stoppages in the work of the mechanism for the implementation of the law, which, as you know, the modern constitutional state cannot afford.

Dissociation of minor satellite from the centromere in mouse

1994 ◽  
Vol 107 (11) ◽  
pp. 3091-3095
Author(s):  
B.K. Vig ◽  
D. Latour ◽  
J. Frankovich
Keyword(s):  
Satellite Dna ◽  
Hoechst 33258 ◽  
Major Satellite ◽  
Primary Constriction ◽  
Minor Satellite ◽  
Antibody Reaction

The minor satellite DNA of mouse is believed to constitute the centromere. We report that centromeres of some chromosomes in the Cl1D cells of mouse are not associated with this DNA even though the latter is present on these chromosomes. The satellite DNA was detected distally from the centromere and could not be mistaken as a component of the centromere. We also report that the site of the primary constriction may not always coincide with the site of the anti-kinetochore antibody reaction. Whereas the regions containing the major satellite decondense upon treatment with bisbenzimidazole (Hoechst 33258), the sites carrying minor satellite resist decondensing.

Chromatin organization in the homogeneously staining regions of a methotrexate-resistant mouse cell line: interspersion of inactive and active chromatin domains distinguished by acetylation of histone H4

1996 ◽  
Vol 109 (9) ◽  
pp. 2221-2228 ◽  
Author(s):  
L. Nicol ◽  
P. Jeppesen
Keyword(s):  
Cell Line ◽  
Dna Sequences ◽  
Satellite Dna ◽  
Mouse Cell ◽  
Histone H4 ◽  
Active Chromatin ◽  
Chromatin Domains ◽  
Resistant Mouse ◽  
Major Satellite ◽  
Drosophila Heterochromatin

We have analyzed the organization of the homogeneously staining regions (HSRs) in chromosomes from a methotrexate-resistant mouse melanoma cell line. Fluorescence in situ hybridization techniques were used to localize satellite DNA sequences and the amplified copies of the dihydrofolate reductase (DHFR) gene that confer drug-resistance, in combination with immunofluorescence using antibody probes to differentiate chromatin structure. We show that the major DNA species contained in the HSRs is mouse major satellite, confirming previous reports, and that this is interspersed with DHFR DNA in an alternating tandem array that can be resolved at the cytological level. Mouse minor satellite DNA, which is normally located at centromeres, is also distributed along the HSRs, but does not appear to interfere with centromere function. The blocks of major satellite DNA are coincident with chromatin domains that are labelled by an autoantibody that recognizes a mammalian homologue of Drosophila heterochromatin-associated protein 1, shown previously to be confined to centric heterochromatin in mouse. An antiserum that specifically recognizes acetylated histone H4, a marker for active chromatin, fails to bind to the satellite DNA domains, but labels the intervening segments containing DHFR DNA. We can find no evidence for the spreading of the inactive chromatin domains into adjacent active chromatin, even after extended passaging of cells in the absence of methotrexate selection.

Isolation and characterization of six different chicken actin genes

1984 ◽  
Vol 4 (11) ◽  
pp. 2498-2508
Author(s):  
K S Chang ◽  
W E Zimmer ◽  
D J Bergsma ◽  
J B Dodgson ◽  
R J Schwartz
Keyword(s):  
Dna Sequences ◽  
Genomic Dna ◽  
Genomic Library ◽  
Smooth Muscle Actin ◽  
Actin Gene ◽  
Muscle Actin ◽  
Repeated Dna ◽  
Alpha Smooth Muscle Actin ◽  
Actin Genes ◽  
Actin Isoform

Genes representing six different actin isoforms were isolated from a chicken genomic library. Cloned actin cDNAs as well as tissue-specific mRNAs enriched in different actin species were used as hybridization probes to group individual actin genomic clones by their relative thermal stability. Restriction maps showed that these actin genes were derived from separate and nonoverlapping regions of genomic DNA. Of the six isolated genes, five included sequences from both the 5' and 3' ends of the actin-coding area. Amino acid sequence analysis from both the NH2- and COOH-terminal regions provided for the unequivocal identification of these genes. The striated isoforms were represented by the isolated alpha-skeletal, alpha-cardiac, and alpha-smooth muscle actin genes. The nonmuscle isoforms included the beta-cytoplasmic actin gene and an actin gene fragment which lacked the 5' coding and flanking sequence; presumably, this region of DNA was removed from this gene during construction of the genomic library. Unexpectedly, a third nonmuscle chicken actin gene was found which resembled the amphibian type 5 actin isoform (J. Vandekerckhove, W. W. Franke, and K. Weber, J. Mol. Biol., 152:413-426). This nonmuscle actin type has not been previously detected in warm-blooded vertebrates. We showed that interspersed, repeated DNA sequences closely flanked the alpha-skeletal, alpha-cardiac, beta-, and type 5-like actin genes. The repeated DNA sequences which surround the alpha-skeletal actin-coding regions were not related to repetitious DNA located on the other actin genes. Analysis of genomic DNA blots showed that the chicken actin multigene family was represented by 8 to 10 separate coding loci. The six isolated actin genes corresponded to 7 of 11 genomic EcoRI fragments. Only the alpha-smooth muscle actin gene was shown to be split by an EcoRI site. Thus, in the chicken genome each actin isoform appeared to be encoded by a single gene.

Solid C60 – How we Found It

1990 ◽  
Vol 206 ◽  
Author(s):  
Donald R. Huffman ◽  
Wolfgang Krätschmer
Keyword(s):  
Gas Phase ◽  
Mass Spectra ◽  
Scanning Tunneling ◽  
Spectral Features ◽  
Small Particles ◽  
Tunneling Microscopy ◽  
X Ray ◽  
Different Types ◽  
Interstellar Material ◽  
The Moment

ABSTRACTIn May of 1990, crystals of a new form of carbon were observed crystallizing from a solution of the newly-produced macroscopic quantities of fullerene molecules. This discovery followed many years of work we had done in producing and measuring the optical properties of small particles of graphitic carbon, a work which was aimed, in part, at a better understanding of the various spectral features of interstellar material. Among three distinctly different types of carbon particulates, distinguished by their differing ultraviolet and Raman spectra, was one that later proved to contain macroscopic quantities of C60 and C70. Evidence for fullerenes came from mass spectra, infrared spectra, and X-ray and electron diffraction. More, recent experiments with our collaborators have included scanning tunneling microscopy and gas phase emission spectroscopy in the infrared. At the moment, there is no obvious connection with interstellar spectral features.

Chromosomal distribution of the major satellite DNA of South American rodents of the genus Ctenomys

1995 ◽  
Vol 69 (3-4) ◽  
pp. 179-184 ◽  
Author(s):  
M.S. Rossi ◽  
C.A. Redi ◽  
G. Viale ◽  
A.I. Massarini ◽  
E. Capanna
Keyword(s):  
Satellite Dna ◽  
South American ◽  
Chromosomal Distribution ◽  
Major Satellite

scholarly journals The calculation, design and optimization of sandwich panels using author’s programs of the automated designing of development

2018 ◽  
Vol 251 ◽  
pp. 04006
Author(s):  
Stanislav Petrov ◽  
Irina Kuznetsova ◽  
Yuri Doladov ◽  
Nikita Krasnov
Keyword(s):  
Sandwich Panels ◽  
Middle Layer ◽  
Russian Language ◽  
Design And Optimization ◽  
Cost Criterion ◽  
Different Types ◽  
The Moment ◽  
The Cost ◽  
Program Interface ◽  
Building Engineering

Three-layer sandwich panels are widely used in insulation of walls and roofing of buildings and other various structures. At the moment building products markets are full of various types of panels by produced different manufactures but skinned with one and the same material only. Panels skinned with two different types of materials are widely used in the sphere of transport. It may be also of considerable economical effect in building engineering. The article presents an analysis of the current state of the problem of calculation of thin-walled profiles in load-bearing structures. The authors developed a program of automated calculation of three-layer panels. The program is certified in Russia. The program allows you to optimize the panel parameters according to the cost criterion. The article presents the basic calculation ideas incorporated in the algorithm of the program. The figures show the program interface. To date, the program has only one Russian language interface. The paper introduces automated methods of singleand multi-span sandwich panels trial design. Different types of materials can be used while skinning these panels. Their middle-layer shift and compliance of supporting structures are taken into account.

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