scholarly journals Phytochemical analysis, phytotoxic and alpha amylase inhibition activity of Cyanodondactylon

2019 ◽  
Vol 8 ◽  
pp. 10-14
Author(s):  
Khaga Raj Sharma
Keyword(s):  
Enzyme Inhibition ◽  
Plant Extract ◽  
Methanolic Extract ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Moderate Activity ◽  
Standard Protocol ◽  
Inhibition Activity ◽  
Phytotoxic Activity

Herbal medicines that obtained from medicinal plants are safe and oldest natural products used for treatment of different diseases. The present study was undertaken to evaluate the phytochemical analysis, phytotoxic activity and alpha amylase enzyme inhibition activity of methanolic extract of Cyanodondactylon. For this whole plant was collected and shade dried and grinded to get the powder. The extraction was carried out by cold percolation in methanol. The methanolic extract was subjected to phytochemical analysis for the absence or presence of secondary metabolites using standard protocol. In-vitro phytotoxic activity was performed by adopting the standard protocol. The alpha amylase enzyme inhibition activity of plant extract was carried out by using starch as substrate, pancreatic alpha amylase as the enzyme, and acarbose as standard. Phytochemical analysis displayed the presence of different chemical constituents such as alkaloids, flavonoids, tannins, polyphenols, terpenoids etc. The result of in-vitro phytotoxic bioassay revealed that the plant extract showed moderate activity with percentage growth regulation 75% in 1000 µg/ml and 30% in 10 µg/ml. The alpha amylase enzyme inhibition was 96.2% at 1000 µg/ml while 37.06% at 40 µg/ml concentration. The inhibition was found dose dependent.

scholarly journals In-vitro antioxidant, antidiabetic and toxic effect of Ageratum houstonianum from Chitwan district Nepal

2020 ◽  
Vol 9 (1) ◽  
pp. 48-54
Author(s):  
Khaga Raj Sharma
Keyword(s):  
Toxic Effect ◽  
Enzyme Inhibition ◽  
Plant Extract ◽  
Methanolic Extract ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Moderate Activity ◽  
Dependent Manner ◽  
Phytotoxic Activity

 Medicinal plants are safe and the oldest natural products used for many years to conserve food, to treat health disorders and to prevent diseases. The active chemical compounds formed during secondary vegetal metabolism is usually responsible for the biological properties of some plant species used throughout the world for various purposes including treatment of diabetes, cancer, infectious diseases etc. The present study was undertaken to analyze the phytochemicals by colour differentiation method, to evaluate the toxic effect by phytotoxic assay, antidiabetic activity by α amylase enzyme inhibition and antioxidant potential by DPPH (2,2-diphenyl-1-picrylhydrazyl) scavenging activity of methanolic extract of Ageratum houstonianum. Treatment of problem in carbohydrate uptake needed the inhibition of α-amylase plays a role in the digestion of polysaccharide and glycogen, is made a strategy for controlling diabetes. For this study whole plant was collected, dried and the powder was made. The extraction was carried out by cold percolation in which methanol was used as a solvent. The methanolic extract was subjected to In-vitro phytotoxic activity by adopting the standard protocol. The α-amylase enzyme inhibition activity of plant extract was carried out by using starch as substrate, pancreatic α amylase as the enzyme, and acarbose as standard. The result of in-vitro phytotoxic bioassay revealed that the plant extract showed moderate activity with percentage growth regulation 80 and 25 percent in a concentration-dependent manner. The α-amylase enzyme inhibition was 74.13 to 99.39 percent in a dose-dependent manner. The antioxidant potential of Ageratum houstonianum extract showed mild activity with IC50 123.67 μg/ml as compared to the standard ascorbic acid IC50 5.38 μg/ ml. It is concluded from the present study that Ageratum houstonianum could be used as a natural source to isolate antioxidant, anti-hyperglycemic agent, herbicide and weedicide as it shows a good α amylase inhibition, radical scavenging and phytotoxic activity respectively.

scholarly journals IN-VITRO ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE INHIBITION ACTIVITY OF DECALEPIS HAMILTONII WIGHT AND ARN

2021 ◽  
pp. 1-3
Author(s):  
Srinivasan S ◽  
Rayar A
Keyword(s):  
Methanolic Extract ◽  
Biologically Active ◽  
Alpha Amylase ◽  
Postprandial Blood Glucose ◽  
Herbal Extracts ◽  
Inhibition Activity ◽  
H Nmr ◽  
Decalepis Hamiltonii ◽  
Alpha Glucosidase

Biologically active polyphenol, D-catechin was isolated from Decalepis hamiltonii and characterized by IR, 1 H- NMR 13C- NMR and evaluation of its in-vitro α-amylase and α-glucosidase inhibition activities. Bioactive compounds are deposited in many parts of the plants, such as in roots, stems, leaves, flowers, fruits and seeds. They protect the plants from diseases and contribute aroma, color and flavor. Inhibitors 𝛼 - amylase and 𝛼-glucosidase delay the breaking down of carbohydrates in the small intestine and lower the postprandial blood glucose excursion. Methanolic extract showed the greater % inhibition of the alpha glucosidase enzyme compared to D-catechin. The herbal extracts produced a slightly weak alpha glucosidase enzyme inhibition when compared with alpha amylase.

scholarly journals Anti-glycation and antioxidant properties of Abutilon indicum plant leaves

2021 ◽  
Vol 3 (1) ◽  
pp. 001-005
Author(s):  
Dinesha Ramadas ◽  
Santhosh Kumar Nune ◽  
Chikkanna D ◽  
Maheshwara KV ◽  
Vedamurthy Joshi
Keyword(s):  
Glucose Uptake ◽  
Enzyme Inhibition ◽  
Antioxidant Properties ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Plant Leaves ◽  
Inhibition Studies ◽  
Abutilon Indicum

The study was done to investigate the in vitro anti-diabetic activity of Ethanol-water extract of Abutilon indicum plant leaves. The phytochemicals present in the extract was analyzed by using standard methods. In vitro antioxidant study was done using DPPH radical scavenging activity. The In vitro anti-diabetic studies were done by alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The phytochemical analysis showed that the extract rich with proteins, polyphenols, flavonoids, alkaloids, tannins, polysaccharides. The in vitro antiglycation potential of extract was confirmed through alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The results of the present study showed the significant antioxidant and antidiabetic activity. The potential pharmacological activity of Abutilon indicum might be the presence of phytochemicals.

scholarly journals Anti-glycation and antioxidant properties of Abutilon indicum plant leaves

2021 ◽  
Vol 2 (2) ◽  
pp. 269-273
Author(s):  
Dinesha Ramadas ◽  
Santhosh Kumar Nune ◽  
Chikkanna D ◽  
Maheshwara KV ◽  
Vedamurthy Joshi
Keyword(s):  
Glucose Uptake ◽  
Enzyme Inhibition ◽  
Antioxidant Properties ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Plant Leaves ◽  
Inhibition Studies ◽  
Abutilon Indicum

The study was done to investigate the in vitro anti-diabetic activity of Ethanol-water extract of Abutilon indicum plant leaves. The phytochemicals present in the extract was analyzed by using standard methods. In vitro antioxidant study was done using DPPH radical scavenging activity. The In vitro anti-diabetic studies were done by alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The phytochemical analysis showed that the extract rich with proteins, polyphenols, flavonoids, alkaloids, tannins, polysaccharides. The in vitro antiglycation potential of extract was confirmed through alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The results of the present study showed the significant antioxidant and antidiabetic activity. The potential pharmacological activity of Abutilon indicum might be the presence of phytochemicals.

Evaluation of Snake Venom’s PhospholipaseA2 Enzyme Inhibition Activity of Cyphostemma adenocoule

2020 ◽  
Vol 14 (3) ◽  
pp. 196-202
Author(s):  
Atul Kaushik ◽  
Teamrat S. Tesfai ◽  
Daniel K. Barkh ◽  
Furtuna K. Ghebremeskel ◽  
Habtom G. Zerihun ◽  
...  
Keyword(s):  
Enzyme Inhibition ◽  
Snake Venom ◽  
Ethanol Extract ◽  
Egg Yolk ◽  
Chloroform Extract ◽  
Inhibition Activity ◽  
Traditional Use ◽  
Snake Bites ◽  
Ethanol Extracts

Background: A snake bite is fundamentally an injury often resulting in puncture wounds meted out by the animal's fangs and occasionally resulting in envenomation. Rate of snake bites around 5,400,000 bites per year leads to over 2,500,000 envenomings and around 125,000 fatal cases annually. Snake venom enzymes are rich in metalloproteinases, phospholipaseA2, proteinases, acetylcholinesterases and hyaluronidases. Objective: Cyphostemma adenocoule is traditionally being used for the treatment of snake bites in Eritrea. The present research was aimed at evaluating the snake venom enzyme inhibition activity of C. adenocoule against puff adder venom and developing a base for the traditional use of the plant against snakebites in Eritrea. Methods: The anti-venom activity of C. adenocoule was assessed in-vitro through phospholipaseA2 enzyme inhibition assay using egg yolk as a cell. The ethanol and chloroform extracts of C. adenocoule showed in vitro anti phospholipase A2 activity, whereas the water extracts of the plant showed no activity. Results: Among the extracts of C. adenocoule, the highest percentage of inhibition was obtained from chloroform extract (95.55% at 100mg/ml). The extract showed prominent activity at different concentrations (34.7% at10mg/ml, 48.8% at 20mg/ml, 54.8% at 40mg/ml, 60.9% at 60mg/ml, 80.5% at 80mg /ml). The ethanol extract also showed certain activity at various concentrations (25.22% at10mg/ml, 14.78% at 20mg/ml, 2.6% at40mg/ml). The activity of the chloroform extracts increases as concentration increases, whereas the activity of the ethanol extracts decreases as concentration increases. The aqueous extract of C. adenocoule did not show any activity at all concentrations. Conclusion: In this study, the chloroform and ethanol extracts of the plant inhibited the enzyme of interest and thus proved the efficacy of anti-snake venom activity of the plant.

Phytochemical analysis of olive flowers' hydroalcoholic extract and in vitro evaluation of tyrosinase, elastase and collagenase inhibition activity

Fitoterapia ◽  
2020 ◽  
Vol 143 ◽  
pp. 104602 ◽  
Author(s):  
Apostolis Angelis ◽  
Panagiotis Mavros ◽  
Panagiota Efstathia Nikolaou ◽  
Sofia Mitakou ◽  
Maria Halabalaki ◽  
...  
Keyword(s):  
Phytochemical Analysis ◽  
Inhibition Activity ◽  
Hydroalcoholic Extract ◽  
In Vitro Evaluation ◽  
Collagenase Inhibition

scholarly journals Alpha-Glucosidase and Alpha-Amylase Inhibitory Activities of Novel Abietane Diterpenes from Salvia africana-lutea

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 421 ◽  
Author(s):  
Etsassala ◽  
Badmus ◽  
Waryo ◽  
Marnewick ◽  
Cupido ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
High Resolution Mass Spectrometry ◽  
Ultra Violet ◽  
Cape Floristic Region ◽  
The Body ◽  
Alpha Amylase ◽  
Inhibitory Activities ◽  
Abietane Diterpenes ◽  
Alpha Glucosidase

The re-investigation of a methanolic extract of Salvia africana-lutea collected from the Cape Floristic Region, South Africa (SA), afforded four new abietane diterpenes, namely 19-acetoxy-12-methoxycarnosic acid (1), 3β-acetoxy-7α-methoxyrosmanol (2), 19-acetoxy-7α-methoxyrosmanol (3), 19-acetoxy-12-methoxy carnosol (4), and two known named clinopodiolides A (5), and B (6), in addition to four known triterpenes, oleanolic, and ursolic acids (7, 8), 11,12-dehydroursolic acid lactone (9) and β-amyrin (10). The chemical structural elucidation of the isolated compounds was determined on the basis of one and two dimensional nuclear magnetic resonance (1D and 2D NMR), high-resolution mass spectrometry (HRMS), ultra violet (UV), fourier transform infrared (IR), in comparison with literature data. The in vitro bio-evaluation against alpha-glucosidase showed strong inhibitory activities of 8, 10, and 7, with the half inhibitory concentration (IC50) values of 11.3 ± 1.0, 17.1 ± 1.0 and 22.9 ± 2.0 µg/mL, respectively, while 7 demonstrated the strongest in vitro alpha-amylase inhibitory activity among the tested compounds with IC50 of 12.5 ± 0.7 µg/mL. Additionally, some of the compounds showed significant antioxidant capacities. In conclusion, the methanolic extract of S. africana-lutea is a rich source of terpenoids, especially abietane diterpenes, with strong antioxidant and anti-diabetic activities that can be helpful to modulate the redox status of the body and could therefore be an excellent candidate for the prevention of the development of diabetes, a disease where oxidase stress plays an important role.

scholarly journals In Vitro Antibacterial, Antifungal and Phytochemical Analysis of Methanolic Extract of Fruit Cassia Fistula

2016 ◽  
Vol 32 (3) ◽  
pp. 1329-1346 ◽  
Author(s):  
Mohanad Jawad Kadhim ◽  
Ghaidaa Jihadi Mohammed ◽  
Imad Hadi Hameed
Keyword(s):  
Methanolic Extract ◽  
Phytochemical Analysis ◽  
Cassia Fistula

scholarly journals Investigating Apoptotic Effects of Methanolic Extract of Dorema glabrum Seed on WEHI-164 Cells

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Maryam Bannazadeh Amirkhiz ◽  
Nadereh Rashtchizadeh ◽  
Hossein Nazemiyeh ◽  
Jalal Abdolalizadeh ◽  
Leila Mohammadnejad ◽  
...  
Keyword(s):  
Plant Extract ◽  
Caspase 3 ◽  
Methanolic Extract ◽  
Spherical Shape ◽  
Dependent Manner ◽  
Chromosomal Dna ◽  
Genes Expression ◽  
Apoptotic Effects ◽  
Cancerous Cells

We aimed to investigate the apoptotic effects of the methanolic extract of Dorema glabrum seed on WEHI-164, cancerous cells in comparison with L929, normal cells and compared them with the cytotoxic effects of Taxol. So, MTT test and DNA fragmentation assay were performed on cultured and treated cells. Also electrophoresis which was followed by immunoblotting was done to survey the production of Caspase-3 and Bcl2 proteins, and to inquire into their relative genes expression, RT-PCR was used. According to our findings, the methanolic extract of Dorema glabrum seed can alter cells morphology as they shrink and take a spherical shape and lose their attachment too. So, the plant extract inhibits cell growth albeit in a time- and dose-dependent manner and results in degradation of chromosomal DNA. Induction of apoptosis by the plant extract was proved by the reduction of pro-Caspase-3 and Bcl2 proteins and increase in Caspase-3 gene expression and decrease in that of bcl2 too. Our data well established the antiproliferative effect of methanolic extract of Dorema glabrum seed and clearly showed that the plant extract can induce apoptosis and not necrosis in vitro. These results demonstrated that Dorema glabrum seed might be a novel and attractive therapeutic candidate for tumor treatment.

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