scholarly journals Anti-glycation and antioxidant properties of Abutilon indicum plant leaves

2021 ◽  
Vol 2 (2) ◽  
pp. 269-273
Author(s):  
Dinesha Ramadas ◽  
Santhosh Kumar Nune ◽  
Chikkanna D ◽  
Maheshwara KV ◽  
Vedamurthy Joshi
Keyword(s):  
Glucose Uptake ◽  
Enzyme Inhibition ◽  
Antioxidant Properties ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Plant Leaves ◽  
Inhibition Studies ◽  
Abutilon Indicum

The study was done to investigate the in vitro anti-diabetic activity of Ethanol-water extract of Abutilon indicum plant leaves. The phytochemicals present in the extract was analyzed by using standard methods. In vitro antioxidant study was done using DPPH radical scavenging activity. The In vitro anti-diabetic studies were done by alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The phytochemical analysis showed that the extract rich with proteins, polyphenols, flavonoids, alkaloids, tannins, polysaccharides. The in vitro antiglycation potential of extract was confirmed through alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The results of the present study showed the significant antioxidant and antidiabetic activity. The potential pharmacological activity of Abutilon indicum might be the presence of phytochemicals.

scholarly journals Anti-glycation and antioxidant properties of Abutilon indicum plant leaves

2021 ◽  
Vol 3 (1) ◽  
pp. 001-005
Author(s):  
Dinesha Ramadas ◽  
Santhosh Kumar Nune ◽  
Chikkanna D ◽  
Maheshwara KV ◽  
Vedamurthy Joshi
Keyword(s):  
Glucose Uptake ◽  
Enzyme Inhibition ◽  
Antioxidant Properties ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Plant Leaves ◽  
Inhibition Studies ◽  
Abutilon Indicum

The study was done to investigate the in vitro anti-diabetic activity of Ethanol-water extract of Abutilon indicum plant leaves. The phytochemicals present in the extract was analyzed by using standard methods. In vitro antioxidant study was done using DPPH radical scavenging activity. The In vitro anti-diabetic studies were done by alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The phytochemical analysis showed that the extract rich with proteins, polyphenols, flavonoids, alkaloids, tannins, polysaccharides. The in vitro antiglycation potential of extract was confirmed through alpha amylase enzyme, alpha glucosidases enzyme inhibition studies and Glucose uptake in Yeast cells studies. The results of the present study showed the significant antioxidant and antidiabetic activity. The potential pharmacological activity of Abutilon indicum might be the presence of phytochemicals.

scholarly journals In Vitro Antidiabetic Effects and Antioxidant Potential ofCassia nemophilaPods

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Gauhar Rehman ◽  
Muhammad Hamayun ◽  
Amjad Iqbal ◽  
Saif Ul Islam ◽  
Saba Arshad ◽  
...  
Keyword(s):  
Glucose Uptake ◽  
Radical Scavenging Activity ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Antioxidant Potential ◽  
Yeast Cells ◽  
In Vitro Assays ◽  
Dpph Radical ◽  
Dpph Radical Scavenging

The antidiabetic and antioxidant potential of ethanolic extract ofCassia nemophilapod (EECNP) was evaluated by three in vitro assays, including yeast glucose uptake assay, glucose adsorption assay, and DPPH radical scavenging activity. The result revealed that the extracts have enhanced the uptake of glucose through the plasma membrane of yeast cells. A linear increase in glucose uptake by yeast cells was noticed with gradual increase in the concentration of the test samples. Moreover, the adsorption capacity of the EECNP was directly proportional to the molar concentration of glucose. Also, the DPPH radical scavenging capacity of the extract was increased to a maximum value of 43.3% at 80 μg/ml, which was then decreased to 41.9% at 100 μg/ml. From the results, it was concluded that EECNP possess good antidiabetic and antioxidant properties as shown by in vitro assays.

scholarly journals Anti-hyperglycaemic and Mode of Action of Thaumatococcus danielli (BENN.) BENTH Ethanol Leave Extract in Streptozotocin-induced Diabetic Rats

2019 ◽  
pp. 1-10
Author(s):  
Folorunsho A. Ajayi ◽  
Olubukola. S. Olorunnisola ◽  
Adewale. Adetutu ◽  
Folashade G. Olorunfemi ◽  
Abiodun O. Owoade ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Uptake ◽  
Mode Of Action ◽  
Inhibitory Effect ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Standard Drug ◽  
Uptake Inhibition ◽  
Enzymatic Glycosylation

Thaumatococcus danielli (Benn.) Benth, a member of the Maranthaceae family has continued to be of immense benefit to the people in the tropics especially in Nigeria. The leaf is widely used among the “Yoruba’s” as a wrapping leaf and for the management of diabetes mellitus. Aim: This study, evaluated the anti-diabetic and possible mode of action(s) of ethanol leaves extract of Thaumatococcus danielli using in vivo and in vitro approach. Methods: Diabetes was induced in Albino rats by administration of Streptozotocin (65 mg/kg/b.wt, i.p). The ethanol leave extract of Thaumatococcus danielli (at a dose of 250 mg/kg and 500 mg/kg body weight was administered at single dose per day to diabetes induced rats for a period of 14 days. The possible mode of action of extract was assessed through in vitro inhibitory effect on alpha amylase, non-enzymatic glycosylation of haemoglobin and glucose uptake in yeast cell. Results: The results showed that the plant extracts demonstrated dose and time dependent reduction in blood glucose. The extract at 250 mg/kg /b.wt and 500 mg/kg/b.wt caused a significant percentage reduction (35.00%/42.04% and 42.16%/60.43%) in blood glucose when compared with the group treated with (25 mg/kg/b.wt) of the standard drug (30.51/40.88%) and the diabetic control (10.46%/-13.67%) on day 7 and day 14 respectively. Although, the extract demonstrated significant (p<0.05) dose dependent inhibitory effect on alpha amylase with an IC50 of 837.97 µg/ml, its activity was significantly (P<0.05) lower than the standard Acarbose. Conversely, the extract showed stronger inhibition of non-enzymatic glycosylation of haemoglobin (87.51%) and enhance glucose uptake in yeast cells by 85.56% when compared with the standard drug Trolax and Metronidazole respectively. Conclusion: The results of this study revealed that Thaumatococcus danielli (Benth) leaves contain anti-hyperglycaemic agent (s) and its possible mode of action is by promoting glucose uptake, inhibition of non-enzymatic glycosylation of haemoglobin and alpha amylase activity.

scholarly journals Phytochemical analysis, phytotoxic and alpha amylase inhibition activity of Cyanodondactylon

2019 ◽  
Vol 8 ◽  
pp. 10-14
Author(s):  
Khaga Raj Sharma
Keyword(s):  
Enzyme Inhibition ◽  
Plant Extract ◽  
Methanolic Extract ◽  
Alpha Amylase ◽  
Phytochemical Analysis ◽  
Moderate Activity ◽  
Standard Protocol ◽  
Inhibition Activity ◽  
Phytotoxic Activity

Herbal medicines that obtained from medicinal plants are safe and oldest natural products used for treatment of different diseases. The present study was undertaken to evaluate the phytochemical analysis, phytotoxic activity and alpha amylase enzyme inhibition activity of methanolic extract of Cyanodondactylon. For this whole plant was collected and shade dried and grinded to get the powder. The extraction was carried out by cold percolation in methanol. The methanolic extract was subjected to phytochemical analysis for the absence or presence of secondary metabolites using standard protocol. In-vitro phytotoxic activity was performed by adopting the standard protocol. The alpha amylase enzyme inhibition activity of plant extract was carried out by using starch as substrate, pancreatic alpha amylase as the enzyme, and acarbose as standard. Phytochemical analysis displayed the presence of different chemical constituents such as alkaloids, flavonoids, tannins, polyphenols, terpenoids etc. The result of in-vitro phytotoxic bioassay revealed that the plant extract showed moderate activity with percentage growth regulation 75% in 1000 µg/ml and 30% in 10 µg/ml. The alpha amylase enzyme inhibition was 96.2% at 1000 µg/ml while 37.06% at 40 µg/ml concentration. The inhibition was found dose dependent.

scholarly journals Evaluating the antidiabetic and antioxidant properties of 5- benzyl-1,3,4-oxadiazole-2-thiol

2021 ◽  
Vol 18 (5) ◽  
pp. 1095-1100
Author(s):  
Shaista Qamar ◽  
Khalid Hussain ◽  
Nadeem I. Bukhar ◽  
Sabahat Z. Siddique ◽  
Aziz-ur-Rehman ◽  
...  
Keyword(s):  
Vitamin C ◽  
Glucose Uptake ◽  
Antioxidant Properties ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Yeast Cells ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Inhibition Activity ◽  
Glycosylation Inhibition

Purpose: To evaluate 5-Benzyl-1,3,4-oxadiazole-2-thiol (OXPA) for antidiabetic and antioxidant properties. Methods: Antidiabetic activity was evaluated using three in vitro models, glucose uptake by yeast cells, alpha amylase inhibition assay and hemoglobin glycosylation inhibition assays. Antioxidant potential was determined by DPPH radical scavenging, reducing power and lipid peroxidation assays. Results: OXPA showed antidiabetic activity in all the three models. The activity of the compound was comparable with that of metronidazole in glucose uptake by yeast cells, but the alpha amylase inhibition activity of the compound was slightly lower than that of acarbose, whereas the hemoglobin glycosylation inhibition activity of the compound was higher than that of vitamin E. DPPH free radical and hydrogen peroxide scavenging activity of the compound was comparable with that of vitamin C. In reducing power assay, the activity of the compound was lower than that of vitamin C (p > 0.05). Conclusion: The results of antidiabetic and antioxidant activity indicate that OXPA may be a drugcandidate for treating both diabetes and its associated oxidative stress.

scholarly journals Chemical Composition of the Essential oil of The Leaves of Pimenta diocia (L.) Merr. & Pimenta racemosa (Mill.) cultivated in Egypt and Evaluation of Their in-vitro Antioxidant and Antidiabetic Activities

2019 ◽  
Vol 10 (4) ◽  
pp. 226
Author(s):  
Heba Abd-Elhady El-gizawy ◽  
Yasser Omar Mosaad ◽  
Naglaa Abd El Khalik Gobba ◽  
Mohammed Abdalla Hussein
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Glucose Uptake ◽  
Antioxidant Properties ◽  
Thiobarbituric Acid ◽  
Positive Control ◽  
Yeast Cells ◽  
Antioxidant Power ◽  
Pimenta Racemosa

<p>The aim of the study is to identify and characterize the chemical composition of the essential oil of both leaves of <em>Pimenta diocia</em> (L.) Merr. and <em>Pimenta racemosa</em> (Mill.)  as well as to evaluate their <em>in-vitro</em> antioxidant and anti-diabetic potency. Both leaves essential oil was analyzed by GC-MS analysis. Different <em>in-vitro</em> antioxidant tests were employed, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH), ferric thiocyanate (FTC), ferric reducing antioxidant power, thiobarbituric acid (TBA) and β-carotene-linoleate bleaching assay. Also, the present work aims to evaluate the α-amylase and α-glucosidase inhibition as well as glucose uptake by yeast cells of essential oils. Essential oil analysis of the leaves of <em>Pimenta racemosa</em> (Mill.) showed high amounts of eugenol (37.95%), β-Myrecene (21.01%), α-Pinene (17.82%), linalool (6.15%) and limonene (5.93%).  GC-MS data of leaves essential oil revealed the presence of  eugenol (30.17%), limonene (17.24),  α-Pinene (16.78%), linalool (9.71), 1,8 cineole (8.31%) and β-myrecene (5.21%) . <em>Pimenta</em> <em>diocia</em> (L.) Merr. and <em>Pimenta</em> <em>racemosa</em> (Mill.) leaves essential oil having antioxidant effect using various methods. In most of the oil samples and assays the antioxidant activity was higher than the one revealed by the positive control BHT. Both plants essential oil showed potent inhibition of α-Amylase at concentration 2.00 mg as it was inhibited by (75 and 63 %); with IC<sub>50</sub> (0.95 and 1.13); respectively as well as inhibition of α-glucosidase enzyme by (61.42 and 53.00%) with IC<sub>50</sub> (3.17 and 4.25); respectively. Also the percentage of glucose uptake by <em>Pimenta diocia</em> (L.) Merr. and <em>Pimenta racemosa</em> (Mill.) leaves essential oil at 2.00 mg/ml in the presence of 25 mM glucose<strong> </strong>is (63.49% and 49.61%); respectively. Conclusion: the present study clearly identified the Egyptian chemotype of <em>Pimenta diocia</em> (L.) Merr. and <em>Pimenta racemosa</em> (Mill.) leaves essential oil, it also displayed safe and promising antidiabetic and antioxidant properties. Therefore the essential oil of both species can be utilized as a natural antioxidant and antidiabetic as well as health benefits.</p>

scholarly journals In Vitro Inhibition of α-Amylase and α-Glucosidase by Extracts from Psiadia punctulata and Meriandra bengalensis

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Yosief Kidane ◽  
Temesgen Bokrezion ◽  
Jimmy Mebrahtu ◽  
Mikias Mehari ◽  
Yacob Berhane Gebreab ◽  
...  
Keyword(s):  
Thin Layer ◽  
Glucose Uptake ◽  
Inhibitory Activity ◽  
Ethanol Extract ◽  
Yeast Cells ◽  
Phytochemical Analysis ◽  
Distilled Water ◽  
Inhibition Activity ◽  
Toxicological Studies

Background. This research assessed the in vitro antidiabetic activity and phytochemical constituents of the traditionally used medicinal plants, Psiadia punctulata and Meriandra bengalensis. Method. The leaves of both plants were subjected to cold extraction method using 70% ethanol and hot Soxhlet extraction using n-hexane, chloroform, methanol, and distilled water. The extracts were studied for their effect on glucose transport across yeast cells and inhibition of α-amylase and α-glucosidase enzyme activities. Thin-layer chromatographic analysis of ethanol extract was also undertaken. Results. The results of yeast glucose uptake assay revealed that extracts from both plants had a maximum increase in glucose uptake at the 25mM glucose concentration with a maximum dose of 2000μg/ml plant extract. The ethanol extract of P. punctulata and aqueous extract of M. bengalensis showed a high activity of 68% and 96%, respectively, at 25mM and 2000μg/ml of glucose and extract concentration. P. punctulata exerted peak inhibition activity of α-amylase of 37.5 ± 3% mg/dl (IC50 = 0.523 mg/dl) for methanol and distilled water extract at 0.5 mg/dl, respectively. M. bengalensis methanol extract exhibited the highest inhibition activity of 38 ± 8 % mg/dl (IC50 = 0.543 mg/dl) at 0.5 mg/dl. In the α-glucosidase inhibition assay, the methanolic extract of P. punctulata exhibited the highest inhibitory activity of 17.29 ± 9% mg/dl (IC50 = 0.761 mg/dl) at 0.5mg/dl. The chloroform extract of M. bengalensis had the highest inhibitory activity of 30 ± 5% mg/dl (IC50=0.6mg/dl) at 0.5 mg/dL. Phytochemical analysis of the different extracts of P. punctulata and M. bengalensis revealed the presence of flavonoids, alkaloids, tannins, saponins, phytosterols, and carbohydrates. Thin-layer chromatography analysis of ethanolic extract of both plants indicated presence of 15 and 17 spots for P. punctulata and M. bengalensis respectively. Conclusion. P. punctulata and M. bengalensis extracts have moderate inhibitory activity against pancreatic α-amylase and relatively low inhibitory activities against α-glucosidase. The observed effects may be associated with the presence of flavonoids, saponins, and alkaloids. Additional in vivo analysis, toxicological studies, isolation, and structural characterization of the phytomolecules identified in this study and molecular docking studies should be undertaken.

QUALITATIVE AND QUANTITATIVE PHYTOCHEMICAL SCREENING AND IN VITRO ANTIOXIDANT EVALUATION OF PERGULARIA DAEMIA

2018 ◽  
Vol 24 (2) ◽  
Author(s):  
GITA MISHRA ◽  
HEMESHWER KUMAR CHANDRA ◽  
NISHA SAHU ◽  
SATENDRA KUMAR NIRALA ◽  
MONIKA BHADAURIA
Keyword(s):  
Reducing Sugars ◽  
Antioxidant Properties ◽  
Phytochemical Analysis ◽  
Phytochemical Screening ◽  
Qualitative And Quantitative ◽  
The Family ◽  
Ethanolic Extracts ◽  
Antioxidant Evaluation ◽  
In Vitro Antioxidant Activity

Pergularia daemia belongs to the family Asclepiadaceae, known to have anticancer, anti-inflammatory activity. Aim of the present study was to evaluate qualitative and quantitative phytochemical and antioxidant properties of ethanolic extracts of leaf, stem and root parts of P. daemia . Preliminary phytochemical analysis and in vitro antioxidant properties were evaluated by standard methods. The qualitative phytochemical analysis of P. daemia showed presence of flavonoids, tannins, alkaloid, phytosterol, carbohydrate, phenol, saponin, glycosides, terpenoids, steroids proteins and reducing sugars. Quantitative analysis showed polyphenol, flavonoid, flavonone, flavone and flavonol in P. daemia leaves, stem and root in considerable quantity. The in vitro antioxidant activity of P. daemia clearly demonstrated that leaf, stem and root parts have prominent antioxidant properties and was effective in scavenging free radicals.

Mycobacterial DNA GyrB Inhibitors: Ligand Based Pharmacophore Modelling and In Vitro Enzyme Inhibition Studies

2014 ◽  
Vol 14 (17) ◽  
pp. 1990-2005 ◽  
Author(s):  
Shalini Saxena ◽  
Janupally Renuka ◽  
Variam Jeankumar ◽  
Perumal Yogeeswari ◽  
Dharmarajan Sriram
Keyword(s):  
Enzyme Inhibition ◽  
Pharmacophore Modelling ◽  
Inhibition Studies

Synthesis of 1,2,3,triazole modified analogues of hydrochlorothiazide via click chemistry approach and in-vitro α-glucosidase enzyme inhibition studies

2021 ◽  
Author(s):  
Hina Siddiqui ◽  
M. A. A. Baheej ◽  
Saeed Ullah ◽  
Fazila Rizvi ◽  
Shazia Iqbal ◽  
...  
Keyword(s):  
Click Chemistry ◽  
Enzyme Inhibition ◽  
Inhibition Studies
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