scholarly journals Pengaruh Praperlakuan Dingin Antera terhadap Pembentukan Kalus Cabai Merah Keriting (Capsicum annuum L.) Pretreatment on the Callus Formation of Curly Red Chilli Pepper (Capsicum annuum L.)

2020 ◽  
Vol 4 (2) ◽  
pp. 94-105
Author(s):  
Nida Wafiqah Nabila M Solin ◽  
Dian Adriani ◽  
Zulfahmi Zulfahmi ◽  
Mokhamad Irfan ◽  
Rosmaina Rosmaina

The production of the double haploid plant in vitro through anther culture technique is a plant breeding technique used to obtain pure strain rapidly. A variety of pretreatment has been reported to induce callus and regenerate planlets efficiently. This study aims at describing the influence of cold anther pretreatment towards the callus formation of curly red chili pepper (Capsicum annuum L.). This research was conducted in the laboratory of Genetics and Breeding, Faculty of Agriculture and Animal Science, Universtas Islam Negeri Sultan Syarif Kasim Riau. The explants used are anther of local genotype of curly red chili pepper. The anthers are stored at low temperatures (4 °c) with different time intervals of 0, 24, 48 and 72 hours. The results showed that the percentage of highest callus formation was obtained at 24 and 72 hours length storage, ie 50%. Cold pretreatment of 72 hours anther storage results in a faster callus with a percentage of the highest yellowish white callus color of 17.65% and a compact structure. The cold pretreatment with 72 hours anther storage is the most optimal acceleration in the development stage of anther culture and induces te formation of curly red chili pepper (Capsicum annuum L.) local genotypes.

2019 ◽  
Vol 19 (1) ◽  
pp. 33
Author(s):  
Rudi Tomson Hutasoit ◽  
Hermanu Triwidodo ◽  
Rully Anwar

The Abundance and Diversity of Thrips (Thysanoptera: Thripidae) on Chili (Capsicum annuum L.) and Cayenne (Capsicum frutescens L.) in Bogor. This research aimed to study the abundance and diversity of thrips on chili pepper and cayenne pepper plants. Observation of thrips was conducted on chili pepper and cayenne pepper in fourteen sites in four different locations in Bogor that are: Dramaga, Cibungbulang, Tenjolaya, and Cisarua. Thrips were collected from leaves and flowers from 10 plant samples that had been selected randomly. The thrips were identified and the number of thrips were calculated. The abundance of adults, nymphs, and total of thrips on the flowers of chili pepper was 0.39, 0.01, and 0.40 thrips/flower respectively. Meanwhile, the abundance of adults, nymphs, and total of thrips on the flowers of cayenne pepper was 0.36, 0.02, and 0.38 thrips/flower respectively. The abundance of adults, nymphs, and total of thrips on the leaves of chili pepper was 0.68, 0.12, and 0.81 thrips/twiq respectively. Meanwhile, the abundance of adults, nymphs, and total of thrips on the leaves of cayenne pepper was 0.47, 0.14, and 0.61 thrips/twiq respectively. Four species of thrips were found infesting flowers of chili pepper and cayenne pepper i. e. Thrips parvispinus, T. hawaiiensis, Scirtothrips dorsalis, and Haplothrips gowdeyi.  Species of T. parvispinus, T. hawaiiensis, S. dorsalis belong to suborder of Terebrantia family Thripidae, meanwhile H. gowdeyi belongs to suborder Tubulifera family Phlaeothripidae. T. parvispinus is the most dominant species found infesting flowers and leaves of the chilli pepper and cayenne pepper were 71% and 56 %.


Author(s):  
Hawraa G.H. Salim ◽  
Ekhlas A.J. ElKaaby ◽  
Saadon A. Al-Ajeel ◽  
Manjeet Yadav

Drought stress in plants caused by water deficiency. In Vitro selection with mutation techniques offered stress tolerance in plants. Shoot tips of chilli pepper (Capsicum annuum . L.) exposed for gamma radiations at the doses (0, 10 and 20 Gy). Irradiated shoots were exposed to stress tolerance in the presence of Polyethylene Glycol (PEG 6000) in MS medium. Analysis of variance results for all characters studied were found to be significant, highest dose 20 Gy interaction with 40 g.l-1 of PEG gave highest number 6.2 shoot. explant-1 whereas 10 Gy and 40 g.l-1 of PEG gave highest plant height 6.04 cm. Further, higher accumulation of proline reached 26.97 mg. g-1 at interaction of 20 Gy+ 60 g.l-1 PEG while highest accumulation of CHO found at 20 Gy+ 40 g.l-1 PEG.


Agriculture ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 19
Author(s):  
Dorota Olszewska ◽  
Magdalena Tomaszewska-Sowa

Androgenesis in vitro is a basic method of obtaining haploid plants and DH (doubled haploid) lines of major crops such as potato, rapeseed, tomato, pepper, wheat, maize, and barley, and also many different minor crops and species with lower agricultural impact. Diploid plants appearing among androgenic regenerants are the effect of spontaneous doubling of the chromosome number in haploid cells during an embryo’s early developmental stages and are valuable fully homozygous breeding material. The subject of the presented research is spontaneous diploidization occurring in the development of androgenic, haploid pepper regenerants. In the presented experiment, the formation of diploid seeds was observed in the progeny of an androgenic, haploid plant derived in an anther culture of a hybrid (Capsicum annuum L. ATZ × Capsicum annuum L. `Corno di toro`)F2. Agromorphological and molecular analyses concerned eight diploid plants being progeny of the anther-derived haploid regenerant. Five of the plants constituted a phenotypically balanced group with valuable agromorphological features. Their genetic homogeneity was confirmed using 10 RAPD markers and 16 ISSR markers. Based on the results, it was concluded that anther-derived haploid plants of Capsicum can be the source of diploid, apomictic seeds, and the obtained offspring may constitute genetically stable, valuable breeding material.


2011 ◽  
Vol 33 (5) ◽  
pp. 1559-1570 ◽  
Author(s):  
Teodora Irikova ◽  
Stanislava Grozeva ◽  
Velichka Rodeva

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