Temporal Changes Mediated by Sri Lankan Sponge Haliclona (Soestella) sp Crude Extract in in vitro Phagocytic Activity of Wistar Rats’ Peritoneal Macrophages

Abstract Investigation on the immunoprotective activity of Cu/Zn superoxide dismutase from Humicola lutea 103 AL (HLSOD) in hamsters with transplanted myeloid tumor was performed. Survivability, tumor growth and tumor transplantability were followed. The immune status of tumor-bearing animals, injected with the optimal protective HLSOD dose, was examined during 27 days after tumor transplantation by the following parameters: (i) the number, migration and phagocytic activity of peritoneal macrophages, (ii) the phagocytic activity of blood polymorphonuclear leukocytes (PMNs), (iii) the responsibility in vitro of spleen lymphocytes to T and B cell mitogens. It was established that intraperitoneal inoculation of HLSOD produced a protective effect on the development of tumors. Elongation of the latent time for tumor appearance and inhibition of the tumor growth were observed. The decreased percentage of mortality in early stage of tumor progression was established. Immunological studies on tumor-bearing hamsters (TBH) induced a tem porary immunorestoring effect on the suppressed phagocytic activities of peritoneal macrophages and blood PMNs during the first 14 days of tumor development. Moreover, HLSOD showed an expressed stimulating effect on proliferative activity in vitro of spleen B lymphocytes from healthy and TBH as well. The immunorestoring and protective effect of the enzyme was probably due to improve of the oxidant-antioxidant balance in peritoneal phagocytes. The tem porary character of the effect can be explained with the interference of immunosuppressing factors produced by tumor tissue as well as by the presence of tumor antigens, tumor cells and antigen-antibody complexes in the circulation.

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Immunomodulatory Activities of Dendrophthoe falcata (L.f) Ettingsh in Experimental Animals: In vitro and In vivo Investigations

Journal of Scientific Research ◽

10.3329/jsr.v3i3.7655 ◽

2011 ◽

Vol 3 (3) ◽

pp. 619-630 ◽

Cited By ~ 2

Author(s):

S. P. Pattanayak ◽

P. M. Mazumder

Keyword(s):

Antibody Titer ◽

Phagocytic Activity ◽

Reticuloendothelial System ◽

Peritoneal Macrophages ◽

Immunomodulatory Activity ◽

Hydroalcoholic Extract ◽

Dendrophthoe Falcata ◽

Stimulation Of

In the present study, an attempt was made to screen immunomodulatory activity of the hydroalcoholic extract (HEDF) of Dendrophthoe falcata (L.f.) Ettingsh (Loranthaceae), an Indian Ayurvedic plant, on different arms of the immune system. HEDF was evaluated for immunological function by studying delayed type hypersensitivity (DTH) to sheep RBCs, nitric oxide (NO) release from murine peritoneal macrophages, phagocytic activity of polymorphonuclear (PMN) cells in vitro and reticuloendothelial system in vivo, plaque forming cell response of splenic lymphocytes to sheep erythrocytes, haemagglutination antibody titer and neutrophil adhesion test. Significant increase in NO production by mouse peritoneal macrophages was detected in culture supernatants indicated increased phagocytic activity of macrophages. After post oral administration of HEDF in three doses of 250, 475 and 950 mg/kg body weight, a significant increase in phagocytic activity of PMN cells/reticuloendothelial system, stimulation of neutrophil function and splenic antibody secreting cells, were also noticed. Stimulation of humoral immune response was further observed with elevation in haemagglutination antibody titer. Heightened DTH reaction suggested convincing evidence for activation of cellular immune system. Present study thus confirms the immunomodulatory activity of the hydroalcoholic extract of D. falcata and the immunomodulatory responses were found to be dose dependent manner.Keywords: Dendrophthoe falcata; Antibody titer; Neutrophil adhesion; Phagocytic activity.© 2011 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi:10.3329/jsr.v3i3.7655 J. Sci. Res. 3 (3), 629-640 (2011)

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Effect of Massoia (Massoia aromatica Becc.) Bark on the Phagocytic Activity of Wistar Rat Macrophages

10.20944/preprints201804.0028.v1 ◽

2018 ◽

Author(s):

Triana Hertiani ◽

Agustinus Yuswanto ◽

Sylvia Utami Tunjung Pratiwi ◽

Harlyanti Mashar

Keyword(s):

Phagocytic Activity ◽

Wistar Rats ◽

In Vitro Assay ◽

Phagocytic Index ◽

Dependent Manner ◽

Vitro Assay ◽

Macrophage Cells ◽

In Vivo Assay

Massoia (Massoia aromatica Becc., Lauraceae) bark has been widely used as a component of traditional Indonesian medicine. The indigenous people boil or steam the bark for traditional applications. Our preliminary research revealed the potency of Massoia essential oil and its major compound, C-10 Massoialactone as potential immunomodulator in vitro. However, no scientific evidence regarding its in vivo effects is available. Therefore, this study evaluated the potential immunomodulatory effects of Massoia bark infusion on the nonspecific immune response (phagocytosis) of Wistar rats. The aqueous extract of Massoia bark was obtained by boiling pulverized bark in water, and the C-10 massoialactone content of the extract was determined through Thin Layer Chromatography (TLC) densitometry. For the in vitro assay, macrophages were treated with the freeze-dried infusion at the concentrations of 2.5, 5, 10, 20, or 40 μg/mL media. For the in vivo assay, 2-month-old male Wistar rats were divided into 5 groups. The baseline group received distilled water at the dose of 1 mL/100 g BW with the immunostimulant herbal product “X” administered as the positive control at the dose of 0.54 mL/rat. The treatment groups received the infusion at a dose of 100, 300, or 500 mg/100 g BW. Treatments were given orally every day for 14 days. The ability of macrophage cells to phagocyte latex was determined as phagocytic index (PI) and was observed under microscopy with 300 macrophages. The in vitro study revealed that the phagocytic activity of the infusion-treated macrophages significantly increased in comparison with that of the control macrophages in a concentration-dependent manner. Among all treatment concentrations, the concentration of 40 μg/ml provided the highest activity with a PI value of 70.51% ± 1.11%. The results of the in vivo assay confirmed those of the in vitro assay. The results of the present study indicate that Massoia bark can increase the phagocytic activity of rat macrophage cells. Its potential as a naturally derived immunomodulatory agent requires further study.

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In Vitro Effect of Crude Extract from Traganum Nudatum on Glucose-Uptake in Liver Slices Isolated from Westar Rats

Medical Technologies Journal ◽

10.26415/2572-004x-vol4iss2p550-551 ◽

2020 ◽

Vol 4 (2) ◽

pp. 550-551

Author(s):

Faiza Mouderas

Keyword(s):

Glucose Uptake ◽

Crude Extract ◽

Wistar Rats ◽

Culture Media ◽

Chronic Hyperglycemia ◽

Liver Slices ◽

Insulin Sensitizers ◽

The Media ◽

Vitro Effect

Background: Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia resulting from defects in insulin secretion, insulin action, or both. There are many classes of drugs used for treatment, and these include insulin sensitizers, insulin secretagogues, and agents that delay the absorption of carbohydrates from the bowel. This study intends to investigate the effect of crude extract from a plant from South Algeria Traganum nudatum (Chenopodiaceae) on glucose uptake in liver slices isolated from Wistar rats. Methods: The liver slices were incubated for 90 min at 37° in normoglycaemic (1g/l of glucose) and hyperglycaemic (3g/l of glucose) KRBA Krebs Ringer Bicarbonate Albumin 4% media using 24 well-polyethylene plates. In each, well different concentrations of insulin (10, 50 and 100µU/ml) and hydromethanolic crude extract (100, 200 and 500µg/ml) were added. After every 30 minutes, aliquots of the culture media were assayed for the determination of glucose left. Results: Tests showed that the glucose left after 90 minutes in the media which contained insulin at 100µg/ml was the lowest (0.44 and 1.41 )g/l in the normo and hyperglycaemic media respectively, which reflect that insulin at this concentration was the most effective on the stimulation of glucose uptake. The extract had the highest effect at 500µg/ml, the concentrations of glucose left after 90 minutes of incubation were found to be (0.38 and 1.31)g/l in the normoglycaemic and hyperglycaemic media respectively. Conclusion: From the obtained results, it can be concluded that our extract seems to have an insulin-like effect on glucose uptake in liver slices isolated from Wistar rats.

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Interaction between Pseudomonas pseudomallei and cultured rabbit peritoneal macrophages

Canadian Journal of Microbiology ◽

10.1139/m76-193 ◽

1976 ◽

Vol 22 (9) ◽

pp. 1307-1311

Author(s):

Richard A. Kishimoto ◽

Warren C. Eveland

Keyword(s):

Bacterial Strain ◽

Phagocytic Activity ◽

Immune Status ◽

Virulent Strain ◽

Peritoneal Macrophages ◽

Immune Serum ◽

Bacterial Survival ◽

Pseudomonas Pseudomallei ◽

Immune Rabbit

The interaction between two strains of Pseudomonas pseudomallei of different virulence with normal and immune rabbit peritoneal macrophages was compared in vitro. Phagocytic activity of macrophages and bacterial survival within macrophages were dependent upon the virulence of the bacterial strain and the immune status of the macrophages. Virulent bacteria were more resistant than the less virulent strain to phagocytosis and destruction. Immune macrophages were more phagocytic and bactericidal than normal macrophages. Specific immune serum facilitated ingestion and destruction of bacteria by both normal and immune macrophages.

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Ultrastructure and Phagocytic Activity of Rat Peritoneal Macrophages Exposed to Low Temperatures in Vitro

Cryobiology ◽

10.1006/cryo.2000.2267 ◽

2000 ◽

Vol 41 (1) ◽

pp. 66-71 ◽

Cited By ~ 12

Author(s):

H. Salman ◽

M. Bergman ◽

H. Bessler ◽

S. Alexandrova ◽

M. Djaldetti

Keyword(s):

Low Temperatures ◽

Phagocytic Activity ◽

Peritoneal Macrophages

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3. Morphological parameters of the effect of interferon on mouse peritoneal macrophages

Philosophical Transactions of the Royal Society of London Series B Biological Sciences ◽

10.1098/rstb.1982.0115 ◽

1982 ◽

Vol 299 (1094) ◽

pp. 131-133 ◽

Cited By ~ 1

Keyword(s):

Light Microscopy ◽

Phagocytic Activity ◽

Quantitative Data ◽

Phase Contrast ◽

Peritoneal Macrophages ◽

Mouse Fibroblast ◽

Morphological Parameters ◽

Glass Surfaces ◽

Mouse Peritoneal Macrophages

Several macrophage functions are modulated by treatment with homologous interferon (IFN). For example, phagocytic activity of mouse peritoneal macrophages (MPM) is enhanced by moderate concentrations of mouse fibroblast interferon (MuIFN-P) (Rollag & Degré 1981). Spreading of freshly seeded macrophages on glass surfaces is stimulated by macrophage-activating agents (Mörland & Kaplan 1977), IFN inducers (Rabinovitch et al . 1977) and IFN (Schultz et al . 1978). We report here quantitative data on effect of MuIFN-β on the spreading of MPM in vitro . Cells were seeded on glass surfaces in Eagle’s MEM, and spreading was examined after incubation at 37 °C for various periods by phase-contrast light microscopy (p.c.m.). Cells, fixed in 2.5% glutaraldehyde, were scored as round or spread, at least 200 cells in each preparation (Rabinovitch & De Stefano 1973).

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