Convenient Methods for Determination of 5'-Ribonucleotides and their Related Compounds Using Separation by Paper Electrophoresis and Correction on UV-Absorption of Filter Paper Itself.

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

Download Full-text

Quantitative Determination of Diaminocarboxylic Acids and Related Compounds by Rapid Paper Electrophoresis

Analytical Chemistry ◽

10.1021/ac60180a037 ◽

1961 ◽

Vol 33 (12) ◽

pp. 1752-1755 ◽

Cited By ~ 15

Author(s):

M. A. Doran

Keyword(s):

Quantitative Determination ◽

Paper Electrophoresis ◽

Related Compounds

Download Full-text

THE DETERMINATION OF SERUM PROTEIN FRACTIONS ON FILTER PAPER ELECTROPHEROGRAMS BY THE BIURET REACTION, AND SOME OBSERVATIONS ON THE SERUM PROTEINS OF THE ESTROGENIZED IMMATURE PULLET

Canadian Journal of Biochemistry and Physiology ◽

10.1139/o54-022 ◽

1954 ◽

Vol 32 (3) ◽

pp. 189-199 ◽

Cited By ~ 4

Author(s):

W. P. McKinley ◽

W. A. Maw ◽

W. F. Oliver ◽

R. H. Common

Keyword(s):

Serum Protein ◽

Filter Paper ◽

Domestic Fowl ◽

Serum Proteins ◽

Paper Electrophoresis ◽

Protein Fractions ◽

Veronal Buffer

An application of the biuret reaction to the determination of protein fractions on filter paper electropherograms of serum is described. The relative mobilities of the serum protein fractions of the domestic fowl and of man are compared. Values are reported for serum protein fractions as separated by filter paper electrophoresis in a methanolic veronal buffer. Some observations on the serum proteins of the estrogenized immature pullet are reported; and it is tentatively suggested that another fraction as well as serum phosphoprotein appears in the serum of the pullet as a consequence of treatment with estrogen.

Download Full-text

Soil quality. Determination of selected explosives and related compounds

10.3403/30199137 ◽

2013 ◽

Keyword(s):

Soil Quality ◽

Related Compounds ◽

Explosives And Related Compounds

Download Full-text

Cholesterol in Serum and Lipoprotein Fractions

Clinical Chemistry ◽

10.1093/clinchem/2.3.145 ◽

1956 ◽

Vol 2 (3) ◽

pp. 145-159 ◽

Cited By ~ 187

Author(s):

Joseph T Anderson ◽

Ancel Keys

Keyword(s):

Human Serum ◽

Total Cholesterol ◽

Filter Paper ◽

Room Temperature ◽

Paper Electrophoresis ◽

Cholesterol Content ◽

Intraindividual Variability ◽

Detailed Data ◽

The Stability ◽

Source Of Error

Abstract 1. Methods are described for the separation, by paper electrophoresis and by cold ethanol, of α- and β-lipoproteins in 0.1 ml. of serum, with subsequent analysis of cholesterol in the separated portions. 2. It is shown that both methods of separation yield separated fractions containing substantially the same amounts of cholesterol. 3. Detailed data are given on the errors of measurement for total cholesterol and for cholesterol in the separated lipoprotein fractions. 4. Studies are reported on the stability of cholesterol in stored serum and on paper electrophoresis strips. It is shown that simple drying on filter paper causes no change in cholesterol content and yields a product that is stable for many weeks at ordinary room temperature. 5. The sources of variability in human serum cholesterol values are examined and it is shown that spontaneous intraindividual variability is a much greater source of error than the errors of measurement with these methods.

Download Full-text

Liquid Chromatographic Determination of Amiodarone Hydrochloride and Related Compounds in Raw Materials and Tablets

Journal of AOAC International ◽

10.1093/jaoac/77.6.1447 ◽

1994 ◽

Vol 77 (6) ◽

pp. 1447-1453 ◽

Cited By ~ 7

Author(s):

Pauline M Lacrok ◽

Norman M Curran ◽

Wing-Wah Sy ◽

Dennis K J Goreck ◽

Pierre Thibault ◽

...

Keyword(s):

Raw Materials ◽

Chromatographic Determination ◽

Raw Material ◽

Liquid Chromatographic Method ◽

Limit Of Quantitation ◽

Liquid Chromatographic Determination ◽

Liquid Chromatographic ◽

Amiodarone Hydrochloride ◽

Related Compounds

Abstract A liquid chromatographic method for the determination of amiodarone hydrochloride and 10 related compounds in drug raw material and for assay of drug in tablets was developed. The method specifies a 3 jxm Hypersil nitrile column (150 × 4.6 mm), a mobile phase of 1 + 1 acetonitrile–ammonium acetate buffer (0.1 M adjusted to pH 6.0 with 0.1 M acetic acid), a flow rate of 1 mL/min, and detection at 240 nm. The lower limit of quantitation of the related compounds is 0.02% or less. Drug contents in 2 raw material samples were 100.1 and 99.9% and ranged from 98.2 to 99.4% in 3 tablet formulations. Impurity levels in 2 samples of raw material from different manufacturers were ca 0.4%. The presence of 3 of the known related compounds in these samples was confirmed by liquid chromatographymass spectrometry. The method applied to raw materials was evaluated by a second laboratory and found to be satisfactory.

Download Full-text