The Effects of Vasectomy on Testicular Tissue of Mice: Histological Changes and DNA Fragmentation Study

Introduction: We aim to investigate the effect of vasectomy on the histology of the testis as well as to evaluate DNA fragmentation in testicular tissue of male mice. Methods: Bilateral vasectomy was performed on 20 mature male mice; 10 control mice underwent sham-operation. After 6 weeks, the testes were evaluated for histological changes and DNA fragmentation by single cell gel electrophoresis (comet assay). Results: Marked alterations were observed in the testes of vasectomized mice, including degeneration of spermatids, thickened basement membrane, dilatation of the seminiferous tubules, exfoliation of germ cells, reduction in the seminiferous cell population, vacuolated appearance of the epithelium in the tubules and marked interstitial fibrosis. Single cell gel electrophoresis showed a highly significant (P<0.0001) increase in DNA damage among vasectomized mice (46.02%) compared with control group (%27.17) after six weeks of operation. Conclusion: Vasectomy induced deterioration in the seminiferous tubules associated with increased testicular cell’s DNA fragmentation.

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Chromatin supraorganization, DNA fragmentation, and cell death in snake erythrocytes

Biochemistry and Cell Biology ◽

10.1139/o04-108 ◽

2005 ◽

Vol 83 (1) ◽

pp. 15-27 ◽

Cited By ~ 8

Author(s):

Maristela Miyamoto ◽

Benedicto C Vidal ◽

Maria Luiza S Mello

Keyword(s):

Cell Death ◽

Single Cell ◽

Acid Hydrolysis ◽

Dna Fragmentation ◽

Gel Electrophoresis ◽

Cell Biology ◽

Tunel Assay ◽

Snake Species ◽

Single Cell Gel Electrophoresis ◽

Bothrops Alternatus

In nucleate erythrocytes of several vertebrate groups, the frequency and intensity of DNA fragmentation associated with programed cell death vary considerably. Although hemoglobin efficiency may be related to erythrocyte life span, and hemoglobin types and erythrocyte life spans are assumed to vary in reptiles, no data on DNA fragmentation and chromatin organization as related to cell death exist for snakes. In the present study, chromatin supraorganization, DNA fragmentation, and cell death were investigated in four snake species (Crotalus durissus terrificus, Bothrops jararaca, Bothrops alternatus, and Bothrops neuwiedii), which differ in their geographical distribution and habitats, by using image analysis of Feulgen hydrolysis kinetics, the TUNEL assay, single-cell gel electrophoresis, and transmission electron microscopy. Relatively few circulating erythrocytes were found to be simultaneously committed to cell death, although there was some variation among the snake species. Conspicuous nuclear and cytoplasmic organelles suggestive of metabolic activity were seen ultrastructurally in most snake erythrocytes. The DNA of the snake erythrocyte chromatin was much more resistant to Feulgen acid hydrolysis (DNA depurination and breakdown) than that of young adult bullfrog erythrocytes, which had a high frequency and intensity of DNA fragmentation. Of the species studied, B. neuwiedii and C. d. terrificus showed the greatest resistance to Feulgen acid hydrolysis and to the DNA fragmentation, revealed by the TUNEL assay. Although B. neuwiedii also showed the lowest frequency of cells with more damaged DNA in the single-cell gel electrophoresis assay, C. d. terrificus had the highest frequency of damaged cells, possibly because of the abundance of alkaline-sensitive DNA sites. The results for DNA fragmentation and cell death in erythrocytes of B. jararaca and B. alternatus generally differed from those for C. d. terrificus and B. neuwiedii and may reflect differences in the biology of these species selected under different geographical habitats. The differences in erythrocyte cell biology reported here may be related to hemoglobin variants selected in the mentioned snake species and that would lead the cells to different resistances to unfavorable environmental conditions.Key words: erythrocytes, snakes, DNA fragmentation, chromatin supraorganization, cell death.

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Low semen quality and adverse histological changes in testes of adult male mice treated with bee venom (Apis mellifera)

Open Veterinary Journal ◽

10.4314/ovj.v11i1.11 ◽

2021 ◽

Vol 11 (1) ◽

pp. 70-79

Author(s):

Sassia O. Regeai ◽

Salma A. Abusrer ◽

Naema S. Shibani

Keyword(s):

Male Infertility ◽

Adult Male ◽

Semen Quality ◽

Sperm Count ◽

Bee Venom ◽

Reproductive Status ◽

Seminiferous Tubules ◽

Control Group ◽

Male Mice ◽

Histological Changes

Background: Male infertility has been on the rise since the past seven decades. Recently, in Libya, bee venom therapy (BVT) has become a popular method among alternative healthcare practitioners for treating male infertility. However, a literature search did not find any published studies that investigated the use of BVT for infertility treatment. Aim: To investigate the effect of bee venom on the male reproductive status through measurements of semen quality parameters and testicular histological changes in adult male mice. Methods: A total of 48 male mice were randomly divided into three experimental groups (which were subdivided into two subgroups with eight mice each) as follows: control, bee venom sting (BVS), and bee venom injection (BVI). The normal control subgroup mice were not subjected to any treatment, while the vehicle control subgroup mice were injected (i.p.) with 200 μl of 0.9% saline solution. In the BVS-treated subgroups, each mouse was stung by one live bee for five times (BVS-5) or seven times (BVS-7) every third day for 2 or 3 weeks. While each mouse in the BVI-treated subgroups received 23 μg/kg in a dose volume of 200 μl BVIs (i.p.) for five times (BVI-5) or seven times (BVI-7) every third day for 15 or 21 days. Results: The findings of this study showed that repeated bee venom treatment by sting or injection to adult male mice resulted in a significant decline in testosterone levels, sperm count, sperm motility, and a very significant increase in the percentage of abnormal sperm morphology; also, there were harmful testicular histological changes in the structural organization of seminiferous tubules and degenerative changes in the germinal epithelium compared to control group. Conclusion: The results of this study provide evidence for the low semen quality and adverse testicular histological changes in male mice treated with bee venom. Hence, there is a desperate need for educating alternative healthcare practitioners and infertile couples about the harmful effects of BVT on reproductive status.

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Histological Effects of Titanium Dioxide Nanoparticles Size 10 Nm in Mice Testes

Science Journal of University of Zakho ◽

10.25271/2017.5.2.361 ◽

2017 ◽

Vol 5 (2) ◽

pp. 158 ◽

Cited By ~ 1

Author(s):

Imad Taher Abdulla

Keyword(s):

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Testicular Tissue ◽

Germinal Epithelium ◽

Seminiferous Tubules ◽

Control Group ◽

Histological Changes ◽

Low Concentrations ◽

High Concentrations ◽

Histological Effect

In the present study, the histological effect of titanium dioxide nanoparticles (TDN) on testicular tissue of mature Mus musculus mice was investigated. The animals were divided into six groups, control group treated with TDN free saline and five groups treated with TDN as follow 5, 10, 50, 100 and 150 mg/kg B.W. The results showed that TDN has histological effects on testicular tissue like sever congestion, mild edema between seminiferous tubules (STs) and decrease the thickness of germinal epithelium at low concentrations, While, the histological changes at high concentrations involved disturbance in STs diameters, sever edema between STs, sever vaculation in the germinal epithelium and necrosis in spermatogonia, germinal epithelium and Sertoli cells.

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Evaluation of genotoxicity after application of Listerine® on human lymphocytes by micronucleus and single cell gel electrophoresis assays

Toxicology and Industrial Health ◽

10.1177/0748233711410918 ◽

2011 ◽

Vol 28 (3) ◽

pp. 271-275 ◽

Cited By ~ 4

Author(s):

Hasan Türkez ◽

Başak Toğar ◽

Taner Arabacı

Keyword(s):

Single Cell ◽

Gel Electrophoresis ◽

Human Lymphocytes ◽

Oral Care ◽

Control Group ◽

Limited Information ◽

Single Cell Gel Electrophoresis ◽

Test Models ◽

Mouth Rinses

Listerine (LN) is one of the most commonly used mouth rinses worldwide although very limited information is available concerning its genotoxicity. In another view, the biological safety profile of oral care products is frequently assumed on the basis of simplistic test models. Therefore, the present study was undertaken to investigate the in vitro genotoxic potential of LN using micronucleus and single cell gel electrophoresis tests as genetic endpoints. Different concentrations of LN (0–100% of ml/culture, v/v) were applied to whole human blood cultures ( n = 5). The result of the present study showed that there were no statistically significant differences ( p > 0.05) between the control group and the groups treated with LN alone in both analysed endpoints. In conclusion, our result first demonstrated the absence of genotoxicity of LN on human lymphocytes.

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