Features of the biochemical composition of winter fodder plants in the Indigirka river basin

The article describes the value of the feed of winter pastures in the extreme north of Yakutia. Winter pastures of the Momsky district are located in marsh places and are used as horse food. Equis?tum fluviatile grows on pastures. Equis?tum Fluviatile is winter-green plants. Equis?tum Froviatile is of the greatest value for the horses of the Momsky district. The research was carry out in the Chiskhan peasant farm of the Momsky district, in the Ulakhan-Chistay village and at the Mercury peasant farm in the Myandigi village of the Amginsky district of the Republic of Sakha (Yakutia) at the end of November. 2020. Samples of winter feed take from pastures near the floodplain of the Moma River, which consisted mainly of marsh horsetail. In the Amga district, a cereal plant - creeping wheatgrass is the standard fodder for Yakutian horses; We are study the biochemical features of Equis?tum Froviatile in the conditions of the Arctic, which complement and expand ideas about the signs of the accumulation of nutrients in winter feed, depending on the cultivation zone. Winter pastures consisted mainly of Equis?tum fluviatile. In the Momsky district, the indicators are higher in cheese protein - 3.05%, for raw fat by 1.65%, for raw materials of fiber by 13.44% than in the Amginsky district. In the Momsky district, the indicators are higher in macro microelements by 1.5-2 times. It is assumed that plants of the Far North accumulate a significant amount of minerals due to their adaptive properties to growing conditions and have a high ability to absorb mineral compounds from the soil.

Monitoring of bacterial agents isolated from small cattle on the territory of certain regions of the Russian Federation

The paper presents the results of bacterial screening of goat and sheep breeding enterprises in certain regions of Russia (Tver, Moscow, Smolensk regions, as well as the Republic of Mari-El and Tatarstan), conducted in the period from 2018 to 2021. In the course of this work, 556 samples of sectional material (heart, lungs, gastrointestinal tract, spleen, kidneys, liver, lymph nodes, breast, flushes from the genitourinary system, as well as exudate from purulent lesions) were subjected to a comprehensive bacteriological study. As a result of the conducted studies, 1223 isolates belonging to 25 families (111 bacterial species) were isolated and identified (by the method of time-of-flight mass spectrometry MALDI-ToF). According to the data obtained, the incidence of Escherichia coli isolation was 10.95%, Trueperella pyogenes – 5.47%, Staphylococcus aureus – 5.31%, Proteus mirabilis – 4.08%, Mannheimia haemolytica – 4.00%, Enterococcus faecalis – 3.76%, Enterobacter cloacea and Staphylococcus haemolyticus – 3.59%, Streptococcus dysgalactiae – 3.51%, Pasteurella multocida – 3.27%, Acinetobacter lwoffii – 2.78%, Staphylococcus cohnii – 2.61%, Bibersteinia trehalosi – 2.29%, Pseudomonas aeruginosa – 2.12%, Bacillus cereus and Micrococcus luteus – 1.96%, Corynebacterium pseudotuberculosis and Staphylococcus equorum – 1.88%, Aerococcus viridans – 1.80%, Corynebacterium xerosis – 1.72%, Clostridium perfringens, Streptococcus mitis and Streptococcus pyogenes – 1.39%, Staphylococcus chromogenes and Streptococcus entericus – 1.14%, respectively. The incidence of isolation of other types of microorganisms was below 1%. The data obtained indicate the circulation of a wide range of bacteria in goat and sheep breeding enterprises of the Russian Federation, some of which should be positioned as pathogenic flora (for example, Pasteurella multocida, Listeria monocytogenes, Salmonella enteritidis, Salmonella typhimurium, Clostridium perfringens, etc.), some as conditionally pathogenic (Trueperella pyogenes, Staphylococcus aureus, Bibersteinia trehalosi, Mannheimia haemolytica, Pseudomonas aeruginosa, Moraxella bovis, Moraxella bovoculi, etc.), as well as the normal flora of the animal body. It is worth focusing on these data when conducting a survey of livestock enterprises in order to establish an objective epizootic situation, including taking into account the possibility of circulating pathogens of factor diseases.

Results of clinical trials of a trivalent vaccine for horses

We have developed a trivalent vaccine made from the SV/69 strain of the Rhinopneumonia virus (RHIV), the bacteria strains of Salmonella abortus equi BN-12 - the causative agent of Salmonella abortion and Streptococcus equi N-34 - the causative agent of the horse strangles. Preclinical laboratory studies of the immunogenicity of the vaccine in linear and outbred mice showed that the preparation protects against experimental infection with the Rhinopneumonia virus by 88.8%, from the causative agents of Salmonella abortion and horse strangles by 100%. There were carried out clinical trials of a trivalent inactivated vaccine with a centrifuge strain Bacillus subtilis TNP-3 as an immunomodulator in order to specifically prevent salmonella abortion, rhinopneumonia and horse strangles. Clinical trials were carried out on horses in the Central regions of Yakutia. The business output of foals was 50.2% in the republic. The business yield in immunized mares was 76,0 % and in unvaccinated mares only 38,0 % in a controlled production trial at the "Uyruye" agricultural production complex. Consequently, immunization of mares with trivalent vaccine increased the reproduction rate by 38,0 %. Results of extensive production tests on 2,568 heads of herd horses confirmed the data of preclinical laboratory studies of immunogenicity in linear and outbred mice. The trivalent vaccine against rhinopneumonia, salmonella abortion and horse strangles stimulates immunobiological reactivity, induces the synthesis of specific antibodies in high titers and increases the business yield of foals from 18,0 to 38,0 %. The vaccine is highly effective and can provide recovery of disadvantaged settlements.

Impact of genomic selection on health improvement of high-yielding cows

The use of selection in dairy cattle breeding, focused for many decades on increasing milk productivity and technological properties of milk (fats and proteins), has led to the health problems of cows, including a decrease in the reproductive function, the prevalence of lameness, metritis, mastitis, infectious lesions of hooves, ketosis, milk fever and others (on average, from 30,0 % to 70,0 %). Calves, which are born by high-yielding cows in their early postnatal period, are characterized by a high mortality rate due to diarrhea (56,0 %) and respiratory diseases (47,0 %). The mortality of young animals and the forced culling of cows are global problems in the world of dairy farming. As a result, there is an interest in disease resistance breeding of dairy cattle, given that only healthy animals have an economic value, being effective and profitable. The purpose of this article was to provide some information on the global trends in the selection of dairy cattle. It is shown that genomic selection, which is originally used among bulls for their assessment and selection based on the productivity of offspring, is currently used to select female cattle by predicting their own further productivity. At the same time, the current direction of selection is a new group of economically significant breeding traits related, inter alia, to animal health, when all traits are assessed together (milk productivity, fat production, protein production, number of live calves produced, incidence of mastitis, lameness, metritis and other signs). The level of genomic selection's reliability is shown, which is 49,0 %, achieved as a result of developments begun in 2016 on the use of genomic selection, taking into account the indicated signs of health. The task is to improve the reliability of estimates for a wide range of phenotypic traits that contribute to the profitability resulting from keeping dairy cows throughout their productive life.

Anti-inflammatory therapy of viral pneumonia on the model of chicken infectious bronchitis

The effect of the drug Lumantse on the infectious bronchitis virus of chickens was evaluated. Infectious bronchitis of chickens is a highly contagious disease characterized by damage to the respiratory system and reproductive organs in young animals caused by a virus of the genus Gamma-coronavirus. The condition of the respiratory system caused by the infectious bronchitis virus (IBD) in chickens has much in common with the pathology of the respiratory system caused by SARS-CoV-2 in humans. For broilers, the course of IBD is characterized more often with damage to the respiratory system, manifested by bronchitis, pneumonia, tracheitis, as well as with kidney damage in the case of circulation of nephropathogenic strains. The effectiveness of the virocidal drug Lumantse was tested on 14-day-old cross shawer chickens infected with a ten-fold dose of the Nobilis IB Ma5 vaccine strain against infectious chicken bronchitis. The results of the studies showed that when the immune system is actively stimulated, the inflammatory response increases, with a simultaneous drop in the viral load, which is why a significant percentage of lung inflammation (60%) was observed in the experimental group. Compared with the control group, when using the drug Lumantse, there is a lower number of inflammatory reactions of the lungs, thymus by 40%, as well as the lowest concentration of viral particles in the lungs and intestines.

The effectiveness of complex treatment of acute catarrhal mastitis in cows

The article presents data on the study of the therapeutic efficacy of methods for treating cows with acute catarrhal mastitis. The research was carried out on the basis of 2 cattle farms SPK (collective farm) "Kolos" Myasnikovsky district of Rostov region and individual entrepreneur Head of the peasant (farm) farm AA Berekchiyan Myasnikovsky district of the Rostov region, as well as on the basis of the North Caucasian zonal research veterinary institute - a branch of the Federal State Budgetary Institution "Federal Rostov Agrarian Scientific Center". To study the therapeutic efficacy of the treatment regimen for acute catarrhal mastitis, 2 groups of animals (experimental and control) were also formed according to the principle of analogous pairs, 15 animals each. Control cows were injected with the antibacterial drug amoxicillin LA 15% twice with an interval of 48 hours at the rate of 1 ml of the drug per 10 kg of animal body weight. The drug was administered fractionally, divided into 2 injections. The animals of the experimental group were also injected with the semisynthetic antibiotic amoxicillin LA 15% twice with an interval of 48 hours at the rate of 1 ml of the drug per 10 kg of the animal's body weight. The treatment regimen was supplemented by exposing the animal's body to pulsed low-intensity infrared laser radiation with simultaneous exposure to pulsed incoherent infrared and red radiation and a constant magnetic field in the infiltrate area once a day at the rate of 5 minutes per 10 cm2 of the affected area for 5-7 days. In the course of our research, it was found that therapy for catarrhal mastitis using pulsed low-intensity infrared laser radiation with simultaneous exposure to pulsed incoherent infrared and red radiation and a constant magnetic field in the infiltrate area once a day, as well as the semisynthetic antibiotic amoxicillin LA 15% twice with at intervals of 48 hours showed a high degree of effectiveness. This is confirmed by the rapid recovery of the clinical picture, physiological parameters, and morphological data of the blood of experimental animals. The therapeutic efficiency of the proposed method of treatment was 100%, recovery occurred on average in 3.9 ± 0.15 days. The signs of the inflammatory process disappeared gradually, however, by the 3rd day of treatment, the secretion of milk was completely restored and a significant improvement in the condition of the animals was noted.

Monitoring of mycotoxins in feed of the Krasnodar Territory and their combined effect on the microbiocenosis of the gastrointestinal tract of cattle

Diets for cattle are affected by fungi - producers of mycotoxins: hay, straw, silage, grain, concentrates. Highly productive cattle breeds are more sensitive to mycotoxins. It has been studied that mycotoxins have antibiotic properties, disrupting the biocenosis and functionality of the proventriculus of cattle. This leads not only to a disruption in the nutritional process of animals, but also contributes to a decrease in the enzymatic activity of the rumen microflora and thus facilitates the penetration of other mycotoxins into the animal body. When conducting sanitary and mycological studies of coarse and succulent feed, compound feed and feed additives for cattle, it was found that feed was affected by micromycetes in descending order Mucor sp., Fusarium sp., Aspergillus sp., Candida sp., Penicillium sp. In the study of feeds, it was found that the most frequently recorded mycotoxins in descending order, T-2 toxin, aflatoxin, zearalenonone, ochratoxin A, fumonisin. Monitoring studies found that feed for cattle not containing mycotoxins was 33.4%, containing one mycotoxin 34.8%, two mycotoxins 28.8%, three mycotoxins 3.03%. When conducting scientific and economic experiments, the content of two mycotoxins in feed was diagnosed, the concentration of which for zearalenone was 1.0 mg / kg, for aflatoxin - 0.04 mg / kg. To study the effect of mycotoxins on the microflora of the gastrointestinal tract (proventriculus), an experiment was carried out on 40 bull calves of 7 months of age. The experimental group of animals (20 animals) was given feed contaminated with mycotoxins, the control group received a diet without mycotoxins. the contents of the rumen were collected on days 10, 20, and 30 of the experiment. The clinical picture in experimental animals with combined mycotoxicosis during the experimental period was characterized by a decrease in feed consumption by 10-20%, weight gain by an average of 18-25%, exhaustion, dullness of the coat, and alopecia. The difference between the experimental and control group of lactobacilli was 28.0%; 157.1% and 215.0% log10 CFU, respectively, for study days. Bifidobacteria 13.0%; 86.2% and 192.3% log10 CFU, respectively. The decrease in ciliates in the experimental group in comparison with the control on the 10th day of the experiment was 7.1 thousand units / g, on the 20 day 27.9 thousand units / g, on the 30th day 77.2 thousand units / g. Thus, the content of the main joints of the biocenosis in the rumen of cattle on days 10, 20 and 30 of the experiment was significantly reduced in the experimental group, the diet of which contained the association of mycotoxins. The monitoring carried out indicates that mycotoxins (in descending order) are most often recorded in feed for farm animals in the Krasnodar Territory: T-2 toxin, aflatoxin B1, zearalenone, ochratoxin A, fumonisin. A high contamination of feed and raw materials with molds was established - Mucor sp., Fusarium sp., Aspergillus sp., Candida sp., Penicillium sp. A decrease in the main representatives of the biocenosis actively participating in the digestion of cattle: lactobacilli, bifidobacteria, ciliates, under the influence of the combined effect of mycotoxins, has been established.

Method for determining the viability of eggs of strongylates of the gastrointestinal tract of horses at critically low temperatures in Yakutia

It is known that the climate of Yakutia is much harsh and hard to tolerate by any living organism. It is considered that the peculiarities of adaptation to the conditions of the Far North are determined by the influence of special natural factors, which is a universal phenomenon of influence on all living organisms. Parasitic nematodes are found in all zones of Yakutia and in almost all domestic animals, among which representatives of the Strongylata suborder dominate. As the authors point out, the parasitization of strongylates in the gastrointestinal tract of herd horses causes great damage, since they reduce not only the productivity of animals, but also cause it to be difficult to endure the winter. Even at subzero temperatures, strongylate eggs are able to maintain their viability. In this paper, the authors of the article were able to present material on determining the ability to maintain the viability of strongylate eggs at low temperatures in Yakutia. For the first time, the results on the survival of strongylate eggs at critically low temperatures of the Yakut winter were obtained and interesting results were obtained, which are described in this article.

The influence of the carnitine-containing complex on the morphostructure at the muscle tissue of Japanese quail

The quality of meat is determined by the set of nutrients that the poultry receives. It's possible to assess the effect of feed additives on poultry meat products by studying the morphostructure of muscle tissue. The carnitine-containing complex was administered to quails of the experimental groups throughout the entire growing period from 2 to 80 days of age. For groups 2 and 3, the drug was drunk for 5 consecutive days with a 10-day interval at a dose of 0.25 ml / l and 0.5 ml / l, respectively; 4 and 5 groups - at a dose of 0.25 ml / l and 0.5 ml / l for 5 days with a 5-day interval, group 1 served as a control and received clean drinking water. For morphological examination, samples of muscle tissues of the superficial pectoralis and biceps femoris were prepared according to a standard protocol, measurement and photographic documentation were performed, followed by statistical data processing. As a result, in the experimental groups, there was a tendency to an increase in the cross-sectional area of the muscle fiber of the superficial pectoral muscle, the fibers are more intensely colored, the nuclei are brightly basophilic, well contoured compared to the control. In the study of the biceps femoris in the experimental groups, an increase in the cross-sectional area of the muscle fiber and a decrease in the thickness of the internal perimisia between the beams of the 2nd order were also noted, which indicates a positive effect of the carnitine-containing complex on the development of muscle fibers of the pectoral and femoral muscles. In group 4, the cross-sectional area of the muscle fiber of the superficial pectoral muscle reached 431.67 mkm2, the thickness of the endomysium was 4.87 mkm, and the internal perimisia was 12.54 mkm; the cross-sectional area of the muscle fiber and the thickness of the internal perimisium of the biceps femoris reached 625.24 mkm2 and 31.02 mkm, which significantly distinguishes the development of the studied muscles from similar indicators in other groups of quails and allows us to recommend this scheme for growing quails and obtaining dietary products.

Antibiotic sensitivity of Pseudomonas aeruginosa strains isolated from milk of highly productive dairy cows

Pseudomonas aeruginosa is a free-living bacterium that is conditionally pathogenic in natural conditions, but when ingested by an animal can cause severe infectious diseases, depending on the location and stage of infection, increasing the time of convalescence. It is naturally resistant to a number of widely used antibacterial drugs: fluoroquinolones, tetracyclines, chloramphenicol; it is capable of forming a biofilm. The aim of this work was to assess the level of sensitivity of strains isolated from cow's milk to the main groups of antibiotics with clinically significant anti-pseudomonasal activities. The work provides microbiological monitoring data for 2020-2021. During this period, 350 milk samples were taken from Holstinized black-and-white cows of the dairy direction of various lactation ages. All isolates were characterized by generally accepted phenotypic methods, with confirmation of biochemical properties. A number of antibiotics selected in this work comply with the recommendations of EUCAST. Sensitivity testing of the isolated strains was carried out by the disco-diffuse method. Pseudomonas aeruginosa was detected in 69 milk samples, which accounted for 19.7% of the total number of milk samples. Pseudomonas Aeruginosa was isolated as a monoculture in 42.03% of cases, in association with gram (-) bacteria in 20.29% and gram (+) in 37.68%. The percentage of pigmented strains in our work was 98.55% of all isolated strains, and 1.45% of poorly pigmented. During the research work, it was found that more than 90% of the strains were resistant to cefepime, the rest showed partial resistance. Therefore, it can be recommended for use only after a correction for sensitivity to this drug. The aminoglycoside group drugs, amikacin and gentamicin, had the highest activity - over 90% against the isolated Ps.a. strains. The results obtained indicate that the isolated strains of Ps.a. they showed high resistance to representatives of the cephalosporin group, which is also increasing with respect to fluoroquinolones, which is a serious problem in the spread of antibiotic resistance.