scholarly journals SENSITIZATION TO ALLERGENIC COMPONENTS OF HOUSE DUST MITES IN CHILDREN WITH BRONCHIAL ASTHMA IN KHARKIV REGION

2021 ◽  
Vol 2021 (4) ◽  
pp. 43-49
Author(s):  
V. A. Klymenko ◽  
T. V. Kulik ◽  
V. M. Belous ◽  
O. V. Pyontkovskaya ◽  
O. M. Savvo
Keyword(s):  
Bronchial Asthma ◽  
House Dust ◽  
Medical Center ◽  
Persistent Asthma ◽  
Allergic Diseases ◽  
House Dust Mites ◽  
Molecular Profile ◽  
Dust Mites ◽  
Key Words Bronchial Asthma ◽  
Molecular Components

SENSITIZATION TO ALLERGENIC COMPONENTS OF HOUSE DUST MITES IN CHILDREN WITH BRONCHIAL ASTHMA IN KHARKIV REGION V. A. Klymenko1 , T. V. Kulik1 , V. M. Belous2 , O. V. Pyontkovskaya3 , O. M. Savvo3 1 Kharkiv National Medical University, Kharkiv, Ukraine 2 Ecomed Medical Center, Kharkiv, Ukraine 3 Municipal non-profit enterprise of Kharkiv Regional Council «Regional Children’s Clinical Hospital G1», Kharkiv, Ukraine Abstract. The aim of the work is to determine the molecular profile of sensitization to house dust mites in children with bronchial asthma in the Kharkiv region. Object and methods of study. 42 children aged from 6 to 18 years were under observation. Evaluation of the sensitization profile was performed by the method of multicomponent photometric analysis ALEX. Results and discussion. Boys predominated among the patients — 76 %. Patients had intermittent (46 %), mild persistent (31 %), moderate persistent (15 %) and severe persistent asthma (8 %). Allergic rhinitis was noted in 88 % of patients. Elevated IgE levels were observed in 69 % of patients. The median IgE is 385.50 [122.75; 991.00] kU/L. Sensitization to house dust mites was detected in 23 (54 %) patients, of which monosensitization — 10 %; co-sensitization with other aeroallergens — 44 %. Among pediatric patients in the Kharkiv region, the leading sensitization is sensitization to D. farinae (44 %) and D. pteronyssinus (48 %). Molecular sensitization profile: Der f1 — 38.1 %; Der f2 — 38.1 %; Der p1 — 33.33 %; Der p2 — 38.1 %; Der p5 — 14.29 %; Der p7 — 16.67 %; Der p10 — 9.52 %; Der p20 — 2.38 %; Der p23 — 26.19 %. Only minor components (Der p5, 7, 10, 20 or 21) were found in 7.14 % of patients, which proves the need for molecular diagnosis when prescribing immunotherapy. Among the «new» allergens for our region — Blomia tropicalis — sensitizationin 11.9 %; molecular profile: Blo t1 — 2.38 %; Blot 10 — 9.52 %; Blo t21 — 4.76 %. Isolated cases of sensitization to ticks Glycyphagus domesticus, Tyrophagus putrescentiae, Lepidoglyphus destructor have been identified. Conclusions. Differences in sensitization to molecular components of house dust mites in children of the Kharkiv region were proved . It should be taken into account in the diagnosis and treatment of patients with allergic diseases. Key words: bronchial asthma, sensitization, house dust mites, molecular components.

scholarly journals HDM exposure enhances subsequent responses to OVA-induced food allergy

2021 ◽  
Author(s):  
Jianli Lin ◽  
Desheng Chen ◽  
Kexin Chang ◽  
Dan Li ◽  
Lvxin Guan ◽  
...  
Keyword(s):  
Food Allergy ◽  
House Dust ◽  
Intestinal Barrier ◽  
Allergic Diseases ◽  
Serum Levels ◽  
House Dust Mites ◽  
Lower Body ◽  
Dust Mites ◽  
Barrier Dysfunction ◽  
Control Groups

Abstract HDM (House dust mites) are important environmental trigger factors of airway allergic diseases, the allergens of HDM were detected in the human gut mucosa, which induces local inflammation and increases intestinal permeability. This study tests a hypothesis that house dust mites contribute to the development of OVA (ovalbumin)-induced food allergy. The serum levels of IgE against HDM in patients with food allergy were detected with UniCAP100 (Pharmacia, Uppsala, Sweden); the HDM-induced/the OVA-induced mouse model of food allergy was developed. Difference between 2 groups was determined by Student t test or ANOVA if more than two groups. Compared to the healthy controls, the patients with food allergy have higher levels of serum IgE against HDM. Compared to food allergy alone groups, the levels of IgE against HDM in food allergy with asthma or allergic rhinitis groups were increased significantly. In mouse models, we found that HDM/OVA induced allergy-like symptoms, lower body temperature, and lower body weight. The levels of IgE, IgG1, mMCP-1 (mouse mast cell protease-1), IL-4 and IL-5 in the HDM and HDM + CT (cholera toxin) groups were higher than the control groups, and the levels of IgE, IgG1, IL-4 and IL-5 in the HDM, OVA and HDM + OVA groups were higher than the control groups. The pathology of intestinal tract in the HDM and HDM + CT/the HDM, OVA and HDM + OVA groups were more severe and exhibited more eosinophils than the control groups. Moreover, the prior exposure to HDM induced intestinal barrier dysfunction, and facilitated the development of intestinal allergy in mice. Based on above data and previous researches, we put forward that HDM exposure enhances subsequent responses to OVA-induced food allergy.

HOUSE DUST MITE ALLERGENS: FROM NATIVE EXTRACTS TO MODIFIED PREPARATIONS

2021 ◽  
Author(s):  
Anna Vasilyeva ◽  
Valentina M. Berzhets ◽  
Nina S. Petrova ◽  
Svetlana V. Khlgatian ◽  
Stanislava Yu. Petrova ◽  
...  
Keyword(s):  
House Dust ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Binding Reaction ◽  
Dust Mite ◽  
Allergenic Activity ◽  
Elisa Inhibition

The growth of allergic diseases dictates the need to develop new forms of therapeutic allergens with high immunogenic and low allergenic activity. For many years, our laboratory has been developing drugs for the diagnosis and treatment of house dust mites (HDM) allergies. The purpose of this study is to summarize the results of the five-year development of therapeutic preparations of HDM allergens. During this period, we obtained the following forms of therapeutic allergens: a granular sublingual dosage form of a mixed allergen from Dermatophagoides pteronyssinus (Der.p) and Dermatophagoides farinaе (Der.f) and a succinylated monomeric HDM allergoid Der.p. The physicochemical and immunobiological properties of the obtained preparations were studied by methods: electrophoresis in PAGE in the presence of SDS-sodium, micropoint immunoblot, ELISA, inhibition of the binding reaction of allergen-specific IgE in the sera of patients. The research results showed that the obtained preparations have a reduced allergenic and increased immunogenic activity in comparison with native extracts. The created forms of mite allergens can be further used to treat patients sensitized to HDM of the genus Dermatophagoides.

scholarly journals TUNGAU DEBU RUMAH YANG DITEMUKAN DI KELURAHAN PERKAMIL KECAMATAN PAAL 2 KOTA MANADO

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Regina F. R. De Breving
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Allergic Diseases ◽  
House Dust Mites ◽  
Living Room ◽  
Dust Mites ◽  
Dust Mite ◽  
Descriptive Survey

Abstract: In house dust are a lot of house dust mites that found in damp houses, cotton mattress, pillows, bolsters, and other home furnishings. Sources of dust containing house dust mites are most in the bedroom  especially cotton mattress. Data from distric health centers Paal 2 found that there are many people who suffer from asthma and allergic diseases, coupled with the humid air in Manado City to allow the development of house dust mites in the area. The purpose of this research is to determine the species and density of house dust mites in urban Perkamil Paal 2 districts Manado city. This research method is descriptive survey to obtain data on the species and density of house dust mites by simple random sampling method. The results found 5 species of house dust mites are Dermatophagoides spp, Acarus spp, Glycyphagus destructor, Tarsonemus spp and Cheyletus spp in bedrooms and living room. House dust mite densities obtained an average of 2,33 in bedroom and 2,07 in living room. The conclusion is house dust mite Dermatophagoides spp most commonly found in bedrooms and living room. Keywords: species, density, house dust mite   Abstrak: Dalam debu rumah terdapat banyak tungau debu rumah yang ditemukan pada rumah yang lembab, kasur kapuk, bantal, guling serta perabot rumah yang lain. Sumber debu yang mengandung tungau debu rumah terbanyak adalah debu kamar tidur terutama debu di kasur kapuk. Dari data Puskesmas Kecamatan Paal 2 didapatkan bahwa masih banyak masyarakat yang menderita asma dan penyakit alergi, ditambah lagi dengan udara Kota Manado yang lembab sehingga memungkinkan terjadinya perkembangan tungau debu rumah di daerah tersebut. Tujuan penelitian ini untuk mengetahui jenis dan kepadatan tungau debu rumah di Kelurahan Perkamil Kecamatan Paal 2 Kota Manado. Metode penelitian ini secara survei deskriptif untuk mendapatkan data tentang jenis dan kepadatan tungau debu rumah dengan metode simple random sampling. Hasil yang didapat ditemukan 5 jenis tungau debu rumah yaitu Dermatophagoides spp, Acarus spp, Glycyphagus destructor, Tarsonemus spp dan Cheyletus spp di ruang tidur dan ruang tamu. Kepadatan tungau debu rumah didapatkan rata-rata 2,33 di ruang tidur dan 2,07 di ruang tamu. Kesimpulan yang diambil yaitu tungau debu rumah Dermatophagoides spp paling banyak ditemukan di ruang tidur maupun ruang tamu. Kata kunci: jenis, kepadatan, tungau debu rumah

scholarly journals SURVEI PERILAKU MASYARAKAT TERHADAP POPULASI TUNGAU DEBU RUMAH DI KELURAHAN PERKAMIL KECAMATAN PAAL 2 KOTA MANADO

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Monalisa Rambing
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Public Attitudes ◽  
Allergic Diseases ◽  
Community Action ◽  
House Dust Mites ◽  
Dust Mites ◽  
The Public ◽  
Care Workers ◽  
Dust Mite

Abstract: House dust mite are insects that can cause allergies in susceptible people. Population of dust mites in the home depends on factors such as the level of the sea, an area with a longer summer than rainy season, a variety of animals in the house, the house is dirty and a lot of dust, as well as the temperature and humidity of the area. Manado city has several of the factors. Purpose of the study is to investigate the people’s behavior of the population of house dust mites in Perkamil village sub-district of Paal 2 Manado city. Result obtained was 34,86% which shows that people’s knowledge about house dust mites are still lacking. The result of attitude is 97,09% indicated public attitudes towards the prevention and eradication of house dust mite is good. And result of the public action showed 75,96% of the community action efforts to prevent and eradicate house dust mites is enough. Necessary education from health care workers about house dust mites and allergic diseases, prevention and eradication, so that the knowledge that still low will be better and the action that will be good enough. Keywords : Behavior, Peoples, House Dust Mites   Abstrak: Tungau debu rumah (TDR) adalah serangga yang dapat menyebabkan alergi pada orang yang rentan. Populasi tungau debu di dalam rumah bergantung pada faktor – faktor, seperti  tinggi rendahnya rumah dari permukaan laut, daerah dengan musim panas yang lebih panjang dari musim hujan, adanya berbagai macam binatang di dalam rumah, rumah yang kotor dan banyak debu, serta suhu dan kelembaban daerah tersebut. Kota Manado memiliki beberapa faktor tersebut. Tujuan penelitian adalah untuk mengetahui perilaku masyarakat terhadap populasi tungau debu rumah di Kelurahan Perkamil Kecamatan Paal 2 Kota Manado. Hasil yang didapatkan adalah 34,86% menunjukkan pengetahuan masyarakat Kelurahan Perkamil mengenai Tungau Debu Rumah masih kurang. Untuk sikap didapatkan hasil 97,09% menunjukkan sikap masyarakat terhadap upaya pencegahan dan pemberantasan Tungau Debu Rumah sudah baik. Dan untuk tindakan masyarakat didapatkan hasil 75,96% menunjukkan tindakan masyarakat terhadap upaya pencegahan dan pemberantasan Tungau Debu Rumah sudah cukup. Perlu dilakukan penyuluhan dari petugas kesehatan tentang Tungau Debu Rumah dan penyakit Alergi, cara pencegahan dan pemberantasan, agar pengetahuan masih rendah akan menjadi lebih baik lagi dan tindakan masyarakat yang sudah cukup akan menjadi baik. Kata Kunci : Perilaku, Masyarakat, Tungau Debu Rumah

Bronchial asthma triggered by house dust mites in patients with local allergic rhinitis

Allergy ◽  
2019 ◽  
Author(s):  
Paloma Campo ◽  
Ibon Eguiluz‐Gracia ◽  
María Carmen Plaza‐Serón ◽  
María Salas ◽  
María José Rodríguez ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Bronchial Asthma ◽  
House Dust ◽  
House Dust Mites ◽  
Dust Mites ◽  
Local Allergic Rhinitis

scholarly journals MODERN VIEW ON THE PROBLEM OF HOUSE DUST MITES SENSITIZATION

2013 ◽  
Vol 10 (5) ◽  
pp. 3-12
Author(s):  
O M Kurbacheva ◽  
K S Pavlova ◽  
E A Melnikova
Keyword(s):  
Allergic Rhinitis ◽  
Bronchial Asthma ◽  
House Dust ◽  
House Dust Mites ◽  
Dust Mites ◽  
Vital Activity ◽  
Modern View ◽  
Atopic Bronchial Asthma

Dermatophagoides pteronissinus, Dermatophagoides farinea and their products of vital activity are the main allergenic components of house dust. The only pathogenetic and effective method of treatment of patients with house dust mites induced allergic rhinitis and atopic bronchial asthma according to positions of evidencebased medicine is allergenspecific immunotherapy (ASIT) with house dust mites (Dermatophagoides pteronissinus, Dermatophagoides farinea) allergens. Modern data on prevalence of a sensitization to house dust mites at patients with respiratory manifestations of allergy are presented in the review, questions of carrying out ASIT by various methods with application of the standardized allergens, its efficiency and safety are discussed.

scholarly journals JENIS DAN KEPADATAN TUNGAU DEBU RUMAH PADA BEBERAPA HABITAT DI RUMAH PENDERITA PENYAKIT ALERGI

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Wiska F. Ponggalunggu ◽  
Victor D. Pijoh ◽  
Greta J. P. Wahongan
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Allergic Diseases ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Cross Sectional ◽  
Dust Mite ◽  
Euroglyphus Maynei

Abstract. House dust mites are the most found allergens in homes scattered throughout the world that associated with allergic manifestations in the respiratory tract and skin, such as bronchial asthma, allergic rhinitis and atopic dermatitis. The most common house dust mite is from the family Pyroglyphidae, four of which have a close relationship with the incidence of allergy: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, and Euroglyphus maynei. This study aims to determine the type and density of house dust mites in several habitats in the homes of people with allergic diseases. This study is a descriptive study using cross-sectional method. Samples were dust in allergic patient’s home in Malalayang I, taken from three habitats, namely bed (mattress and bed linen), bedrooms floor and sofa, as many as 96 samples. Of the 96 samples, there are 60 samples positive of dust mites and 36 negative samples. This study found 157 mites (124 adults and 33 larvae mites). Of the 124 adult mites were identified, there are 122 from order Astigmata with the dominant species is Dermatophagoides pteronyssinus, and two others of the order Prostigmata. In each habitat that is in the bed (mattress and bed linen), bedrooms floor and sofa, Pyroglyphidae is the most prevalent.Keywords: house dust mite, allergiesAbstrak. Tungau debu rumah (TDR) adalah alergen dalam rumah terbanyak yang tersebar di seluruh dunia yang dikaitkan dengan manifestasi alergi pada saluran pernapasan dan kulit, seperti asma bronkial, rinitis alergi dan dermatitis atopik. TDR yang paling sering ditemukan adalah dari famili Pyroglyphidae, empat di antaranya memiliki hubungan yang erat dengan kejadian alergi: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, dan Euroglyphus maynei. Penelitian ini bertujuan untuk mengetahui jenis dan kepadatan tungau debu rumah pada beberapa habitat di rumah penderita penyakit alergi. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode cross-sectional. Sampel penelitian adalah debu di rumah penderita alergi di Kelurahan Malalayang I yang diambil dari 3 habitat, yaitu tempat tidur (kasur dan sprei), lantai kamar tidur, dan sofa sebanyak 96 sampel. Dari 96 sampel, 60 sampel positif tungau debu dan 36 sampel negatif. Pada penelitian ini ditemukan 157 tungau (124 tungau dewasa dan 33 larva). Dari 124 tungau dewasa yang terindentifikasi, dari ordo Astigmata sebanyak 122 dengan spesies yang dominan adalah Dermatophagoides pteronyssinus, dan dua lainnya dari ordo Prostigmata. Pada masing-masing habitat yaitu di tempat tidur (kasur dan sprei), lantai kamar tidur dan sofa, famili Pyroglyphidae paling banyak ditemukan.Kata kunci: tungau debu rumah, alergi

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