scholarly journals HDM exposure enhances subsequent responses to OVA-induced food allergy

2021 ◽  
Author(s):  
Jianli Lin ◽  
Desheng Chen ◽  
Kexin Chang ◽  
Dan Li ◽  
Lvxin Guan ◽  
...  
Keyword(s):  
Food Allergy ◽  
House Dust ◽  
Intestinal Barrier ◽  
Allergic Diseases ◽  
Serum Levels ◽  
House Dust Mites ◽  
Lower Body ◽  
Dust Mites ◽  
Barrier Dysfunction ◽  
Control Groups

Abstract HDM (House dust mites) are important environmental trigger factors of airway allergic diseases, the allergens of HDM were detected in the human gut mucosa, which induces local inflammation and increases intestinal permeability. This study tests a hypothesis that house dust mites contribute to the development of OVA (ovalbumin)-induced food allergy. The serum levels of IgE against HDM in patients with food allergy were detected with UniCAP100 (Pharmacia, Uppsala, Sweden); the HDM-induced/the OVA-induced mouse model of food allergy was developed. Difference between 2 groups was determined by Student t test or ANOVA if more than two groups. Compared to the healthy controls, the patients with food allergy have higher levels of serum IgE against HDM. Compared to food allergy alone groups, the levels of IgE against HDM in food allergy with asthma or allergic rhinitis groups were increased significantly. In mouse models, we found that HDM/OVA induced allergy-like symptoms, lower body temperature, and lower body weight. The levels of IgE, IgG1, mMCP-1 (mouse mast cell protease-1), IL-4 and IL-5 in the HDM and HDM + CT (cholera toxin) groups were higher than the control groups, and the levels of IgE, IgG1, IL-4 and IL-5 in the HDM, OVA and HDM + OVA groups were higher than the control groups. The pathology of intestinal tract in the HDM and HDM + CT/the HDM, OVA and HDM + OVA groups were more severe and exhibited more eosinophils than the control groups. Moreover, the prior exposure to HDM induced intestinal barrier dysfunction, and facilitated the development of intestinal allergy in mice. Based on above data and previous researches, we put forward that HDM exposure enhances subsequent responses to OVA-induced food allergy.

HOUSE DUST MITE ALLERGENS: FROM NATIVE EXTRACTS TO MODIFIED PREPARATIONS

2021 ◽  
Author(s):  
Anna Vasilyeva ◽  
Valentina M. Berzhets ◽  
Nina S. Petrova ◽  
Svetlana V. Khlgatian ◽  
Stanislava Yu. Petrova ◽  
...  
Keyword(s):  
House Dust ◽  
Allergic Diseases ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Binding Reaction ◽  
Dust Mite ◽  
Allergenic Activity ◽  
Elisa Inhibition

The growth of allergic diseases dictates the need to develop new forms of therapeutic allergens with high immunogenic and low allergenic activity. For many years, our laboratory has been developing drugs for the diagnosis and treatment of house dust mites (HDM) allergies. The purpose of this study is to summarize the results of the five-year development of therapeutic preparations of HDM allergens. During this period, we obtained the following forms of therapeutic allergens: a granular sublingual dosage form of a mixed allergen from Dermatophagoides pteronyssinus (Der.p) and Dermatophagoides farinaе (Der.f) and a succinylated monomeric HDM allergoid Der.p. The physicochemical and immunobiological properties of the obtained preparations were studied by methods: electrophoresis in PAGE in the presence of SDS-sodium, micropoint immunoblot, ELISA, inhibition of the binding reaction of allergen-specific IgE in the sera of patients. The research results showed that the obtained preparations have a reduced allergenic and increased immunogenic activity in comparison with native extracts. The created forms of mite allergens can be further used to treat patients sensitized to HDM of the genus Dermatophagoides.

scholarly journals TUNGAU DEBU RUMAH YANG DITEMUKAN DI KELURAHAN PERKAMIL KECAMATAN PAAL 2 KOTA MANADO

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Regina F. R. De Breving
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
Allergic Diseases ◽  
House Dust Mites ◽  
Living Room ◽  
Dust Mites ◽  
Dust Mite ◽  
Descriptive Survey

Abstract: In house dust are a lot of house dust mites that found in damp houses, cotton mattress, pillows, bolsters, and other home furnishings. Sources of dust containing house dust mites are most in the bedroom  especially cotton mattress. Data from distric health centers Paal 2 found that there are many people who suffer from asthma and allergic diseases, coupled with the humid air in Manado City to allow the development of house dust mites in the area. The purpose of this research is to determine the species and density of house dust mites in urban Perkamil Paal 2 districts Manado city. This research method is descriptive survey to obtain data on the species and density of house dust mites by simple random sampling method. The results found 5 species of house dust mites are Dermatophagoides spp, Acarus spp, Glycyphagus destructor, Tarsonemus spp and Cheyletus spp in bedrooms and living room. House dust mite densities obtained an average of 2,33 in bedroom and 2,07 in living room. The conclusion is house dust mite Dermatophagoides spp most commonly found in bedrooms and living room. Keywords: species, density, house dust mite   Abstrak: Dalam debu rumah terdapat banyak tungau debu rumah yang ditemukan pada rumah yang lembab, kasur kapuk, bantal, guling serta perabot rumah yang lain. Sumber debu yang mengandung tungau debu rumah terbanyak adalah debu kamar tidur terutama debu di kasur kapuk. Dari data Puskesmas Kecamatan Paal 2 didapatkan bahwa masih banyak masyarakat yang menderita asma dan penyakit alergi, ditambah lagi dengan udara Kota Manado yang lembab sehingga memungkinkan terjadinya perkembangan tungau debu rumah di daerah tersebut. Tujuan penelitian ini untuk mengetahui jenis dan kepadatan tungau debu rumah di Kelurahan Perkamil Kecamatan Paal 2 Kota Manado. Metode penelitian ini secara survei deskriptif untuk mendapatkan data tentang jenis dan kepadatan tungau debu rumah dengan metode simple random sampling. Hasil yang didapat ditemukan 5 jenis tungau debu rumah yaitu Dermatophagoides spp, Acarus spp, Glycyphagus destructor, Tarsonemus spp dan Cheyletus spp di ruang tidur dan ruang tamu. Kepadatan tungau debu rumah didapatkan rata-rata 2,33 di ruang tidur dan 2,07 di ruang tamu. Kesimpulan yang diambil yaitu tungau debu rumah Dermatophagoides spp paling banyak ditemukan di ruang tidur maupun ruang tamu. Kata kunci: jenis, kepadatan, tungau debu rumah

scholarly journals SURVEI PERILAKU MASYARAKAT TERHADAP POPULASI TUNGAU DEBU RUMAH DI KELURAHAN PERKAMIL KECAMATAN PAAL 2 KOTA MANADO

2013 ◽  
Vol 1 (2) ◽  
Author(s):  
Monalisa Rambing
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Public Attitudes ◽  
Allergic Diseases ◽  
Community Action ◽  
House Dust Mites ◽  
Dust Mites ◽  
The Public ◽  
Care Workers ◽  
Dust Mite

Abstract: House dust mite are insects that can cause allergies in susceptible people. Population of dust mites in the home depends on factors such as the level of the sea, an area with a longer summer than rainy season, a variety of animals in the house, the house is dirty and a lot of dust, as well as the temperature and humidity of the area. Manado city has several of the factors. Purpose of the study is to investigate the people’s behavior of the population of house dust mites in Perkamil village sub-district of Paal 2 Manado city. Result obtained was 34,86% which shows that people’s knowledge about house dust mites are still lacking. The result of attitude is 97,09% indicated public attitudes towards the prevention and eradication of house dust mite is good. And result of the public action showed 75,96% of the community action efforts to prevent and eradicate house dust mites is enough. Necessary education from health care workers about house dust mites and allergic diseases, prevention and eradication, so that the knowledge that still low will be better and the action that will be good enough. Keywords : Behavior, Peoples, House Dust Mites   Abstrak: Tungau debu rumah (TDR) adalah serangga yang dapat menyebabkan alergi pada orang yang rentan. Populasi tungau debu di dalam rumah bergantung pada faktor – faktor, seperti  tinggi rendahnya rumah dari permukaan laut, daerah dengan musim panas yang lebih panjang dari musim hujan, adanya berbagai macam binatang di dalam rumah, rumah yang kotor dan banyak debu, serta suhu dan kelembaban daerah tersebut. Kota Manado memiliki beberapa faktor tersebut. Tujuan penelitian adalah untuk mengetahui perilaku masyarakat terhadap populasi tungau debu rumah di Kelurahan Perkamil Kecamatan Paal 2 Kota Manado. Hasil yang didapatkan adalah 34,86% menunjukkan pengetahuan masyarakat Kelurahan Perkamil mengenai Tungau Debu Rumah masih kurang. Untuk sikap didapatkan hasil 97,09% menunjukkan sikap masyarakat terhadap upaya pencegahan dan pemberantasan Tungau Debu Rumah sudah baik. Dan untuk tindakan masyarakat didapatkan hasil 75,96% menunjukkan tindakan masyarakat terhadap upaya pencegahan dan pemberantasan Tungau Debu Rumah sudah cukup. Perlu dilakukan penyuluhan dari petugas kesehatan tentang Tungau Debu Rumah dan penyakit Alergi, cara pencegahan dan pemberantasan, agar pengetahuan masih rendah akan menjadi lebih baik lagi dan tindakan masyarakat yang sudah cukup akan menjadi baik. Kata Kunci : Perilaku, Masyarakat, Tungau Debu Rumah

scholarly journals JENIS DAN KEPADATAN TUNGAU DEBU RUMAH PADA BEBERAPA HABITAT DI RUMAH PENDERITA PENYAKIT ALERGI

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Wiska F. Ponggalunggu ◽  
Victor D. Pijoh ◽  
Greta J. P. Wahongan
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Allergic Diseases ◽  
Dermatophagoides Pteronyssinus ◽  
House Dust Mites ◽  
Dust Mites ◽  
Dermatophagoides Farinae ◽  
Cross Sectional ◽  
Dust Mite ◽  
Euroglyphus Maynei

Abstract. House dust mites are the most found allergens in homes scattered throughout the world that associated with allergic manifestations in the respiratory tract and skin, such as bronchial asthma, allergic rhinitis and atopic dermatitis. The most common house dust mite is from the family Pyroglyphidae, four of which have a close relationship with the incidence of allergy: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, and Euroglyphus maynei. This study aims to determine the type and density of house dust mites in several habitats in the homes of people with allergic diseases. This study is a descriptive study using cross-sectional method. Samples were dust in allergic patient’s home in Malalayang I, taken from three habitats, namely bed (mattress and bed linen), bedrooms floor and sofa, as many as 96 samples. Of the 96 samples, there are 60 samples positive of dust mites and 36 negative samples. This study found 157 mites (124 adults and 33 larvae mites). Of the 124 adult mites were identified, there are 122 from order Astigmata with the dominant species is Dermatophagoides pteronyssinus, and two others of the order Prostigmata. In each habitat that is in the bed (mattress and bed linen), bedrooms floor and sofa, Pyroglyphidae is the most prevalent.Keywords: house dust mite, allergiesAbstrak. Tungau debu rumah (TDR) adalah alergen dalam rumah terbanyak yang tersebar di seluruh dunia yang dikaitkan dengan manifestasi alergi pada saluran pernapasan dan kulit, seperti asma bronkial, rinitis alergi dan dermatitis atopik. TDR yang paling sering ditemukan adalah dari famili Pyroglyphidae, empat di antaranya memiliki hubungan yang erat dengan kejadian alergi: Dermatophagoides pteronyssinus, Dermatophagoides farinae, Dermatophagoides microceras, dan Euroglyphus maynei. Penelitian ini bertujuan untuk mengetahui jenis dan kepadatan tungau debu rumah pada beberapa habitat di rumah penderita penyakit alergi. Penelitian ini adalah penelitian deskriptif dengan menggunakan metode cross-sectional. Sampel penelitian adalah debu di rumah penderita alergi di Kelurahan Malalayang I yang diambil dari 3 habitat, yaitu tempat tidur (kasur dan sprei), lantai kamar tidur, dan sofa sebanyak 96 sampel. Dari 96 sampel, 60 sampel positif tungau debu dan 36 sampel negatif. Pada penelitian ini ditemukan 157 tungau (124 tungau dewasa dan 33 larva). Dari 124 tungau dewasa yang terindentifikasi, dari ordo Astigmata sebanyak 122 dengan spesies yang dominan adalah Dermatophagoides pteronyssinus, dan dua lainnya dari ordo Prostigmata. Pada masing-masing habitat yaitu di tempat tidur (kasur dan sprei), lantai kamar tidur dan sofa, famili Pyroglyphidae paling banyak ditemukan.Kata kunci: tungau debu rumah, alergi

scholarly journals SURVEY PERILAKU MASYARAKAT TERHADAP POPULASI TUNGAU DEBU RUMAH DI KELURAHAN TITIWUNGEN SELATAN KECAMATAN SARIO KOTA MANADO

2013 ◽  
Vol 1 (1) ◽  
Author(s):  
Ihat S. E. Purba ◽  
Victor D. Pijoh ◽  
J. Runtuwene
Keyword(s):  
House Dust ◽  
Physical Environment ◽  
Allergic Diseases ◽  
Total Sample ◽  
House Dust Mites ◽  
Survey Method ◽  
Dust Mites ◽  
Descriptive Research ◽  
Family Based ◽  
Ways Of Life

Abstract: The term House Dust Mites has been applied to a large number of mites found associated with dust in dwellings. This mites can be found in mattresses, pillows, overstuffed furniture and another place where human rest. House Dust Mites can be a important cause of allergic reaction like asthma, rhinitis, conjunctivitis, and atopic  dermatitis. The increase of house dust mites and allergic diseases because some factors such as problems of inadequate treatment of patients, medical officers are less able to correctly diagnose, problems of the physical environment, and different ways of life that may be supported from the socioeconomic individuals. This research are aimed to know the knowledge, atitude, and action of community about House Dust Mites. This research is a descriptive research using survey method. It was conducted in the South Titiwungen Village, Sario Sub-District, Manado with total sample 92 head of  family. Based on the result of research method showed that 56,5% of community have the good knowledge about house dust mites , 98,7%  of community have the good atitude and 89,1% of community also have the good action. Keywords: behavior,  community, house dust mites     Abstrak: Istilah tungau debu rumah telah digunakan untuk menyatakan sejumlah tungau yang ditemukan berasosiasi dengan debu di rumah-rumah tempat tinggal. Tungau ini dapat ditemukan di kasur, bantal, perabot rumah tangga dan di tempat lain dimana manusia beristirahat. Tungau debu rumah merupakan penyebab penting dalam timbulnya penyakit alergi seperti asma,  konjungtivitis, rinithis, dan atopik dermatitis. Terjadinya peningkatan tungau debu rumah dan penyakit alergi ini karena beberapa faktor seperti masalah penanganan penderita yang tidak adekuat, petugas medis kurang mampu mendiagnosis dengan tepat, dan juga masalah faktor lingkungan fisik serta perbedaan cara hidup masyarakat yang kemungkinan di tunjang dari sosioekonomi individu. Penelitian ini bertujuan untuk mengetahui pengetahuan, sikap dan tindakan masyarakat terhadap populasi tungau debu rumah. Penelitian ini merupakan jenis penelitian Deskriptif dengan metode survey. Lokasi di Kelurahan Titiwungen Selatan, Kecamatan Sario dengan jumlah sampel sebanyak 92 KK. Penelitian ini menunjukkan bahwa  56,7% masyarakat memiliki pengetahuan baik, 97,8% masyrakat memiliki sikap baik dan 89,1% tindakan masyarakat juga sudah baik. Kata kunci: perilaku,  masyarakat, tungau debu rumah

scholarly journals SURVEI PERILAKU MASYARAKAT TERHADAP POPULASI TUNGAU DEBU RUMAH DI KELURAHAN BITUNG KARANGRIA KECAMATAN TUMINTING KOTA MANADO

2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Greis Emilia Tawurisi
Keyword(s):  
House Dust ◽  
House Dust Mite ◽  
Health Workers ◽  
Allergic Diseases ◽  
Health Department ◽  
House Dust Mites ◽  
Local Health ◽  
Dust Mites ◽  
The Public ◽  
Dust Mite

Abstract: House dust mites (HDM) is very small creatures and can not visible with the unaided eyes, found in the house dust and can causing allergic diseases. The existence of dust mites can not be separated from people's behavior about the house dust mite populations.The purpose of research is to know of the public behavior about the population House Dust Mites in the Bitung Karangria Village District of Tuminting Manado.The results obtained 34,86% are show that the cognition of the mites in Bitung Karangria Village is still deficient, and for attitude 97.83% are show that people have a good attitude, and for the action are obtained 82,44%, this show the attitude of the community is good.The cognition about house dust mitesis still less, and for the attitudes and actions of society is good. It takes counseling from health workers especially local health department about the house dust mite. Keywords: behavior of the public, House Dust Mites  Abstrak: Tungau debu rumah (TDR) adalah mahluk yang sangat kecil dan  tidak dapat dilihat dengan mata telanjang, ditemukan pada debu rumah dan dapat menyebabkan penyakit alergi. Keberadaan tungau  debu rumah tidak terlepas dari perilaku masyarakat terhadap populasi tungau debu rumah tersebut. Tujuan penelitian adalah Mengetahui perilaku masyarakat terhadap populasi Tungau Debu Rumah di kelurahan Bitung karangria Kecamatan Tuminting kota Manado. Hasil yang didapatkan adalah 34,86% menunjukan pengetahuan masyarakat Kelurahan Bitungkarangria terhadap tungau debu rumah masi kurang, dan sikap didapatkan hasil 97,83% menunjukan bahwa sikap masyarakat sudah baik, serta untuk tindakan didapatkan hasil 82,44% ini menunjukan sikap masyarakat sudah baik. Pengetahuan  masyarakat mengenai tungau debu rumah masi kurang, dan untuk sikap serta tindakan masyarakat sudah baik. Dibutuhkan penyuluhan dari petugas kesehatan kususnya dinas kesehatan setempat mengenai tungau debu rumah. Kata kunci: perilaku masyarakat, Tungau Debu Rumah

scholarly journals Assessment of the cytokine profile in patients with allergic rhinitis caused by sensitization to house dust mite

2020 ◽  
pp. 25-31
Author(s):  
Serhii Yuriev
Keyword(s):  
Allergic Rhinitis ◽  
House Dust ◽  
Serum Levels ◽  
Cytokine Profile ◽  
House Dust Mites ◽  
Dust Mites ◽  
Independent Form ◽  
Dependent Form ◽  
Ige Synthesis ◽  
Th1 Cytokines

The aim of the study was to evaluate the serum cytokine profile of patients with allergic rhinitis (AR) sensitized to house dust mites and to establish their role in the regulation of IgE synthesis Materials and Methods. The study included 60 patients with AR at the age of 20-60. According to the level of total IgE (IgE>100 kU/L), patients were divided into two groups with an IgE-dependent and IgE-independent form of AR. In the study, the serum levels of IL-2, g-IFN, IL-4, IL-5, IL-13, IL-10 and TGF-b in the blood serum were determined by ELISA. Results. According to the study, it was found that patients with an IgE-dependent form of AR are characterized by a decrease in the level of Th1 cytokines IL-2 and g-IFN, an increase in the level of Th2 cytokines – IL-4, IL-5 and IL-13 and a decrease in Treg cytokines – IL-10 and TGF- b. At the same time, a significant decrease in the level of inhibiting cytokine IL-10 was established in comparison with the group of patients with an IgE-independent form of AR. For patients with an IgE-independent form, only elevated levels of IL-13 were found. No significant differences were found between other Th1 and Th2 and Treg cytokines. Conclusions. The study showed that in patients with AR sensitization to house dust mites, IL-13 acts as a key cytokine in the IgE-independent form of AR, which can be important in the future for both predicting and treating AR.

Phylogenetic analysis of house dust mites

Open Medicine ◽  
2010 ◽  
Vol 5 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Yubao Cui ◽  
Cuixiang Gao ◽  
Ying Zhou ◽  
Peng Zhou ◽  
Ming Peng ◽  
...  
Keyword(s):  
Amino Acid ◽  
House Dust ◽  
Phylogenetic Trees ◽  
Allergic Diseases ◽  
Amino Acid Sequences ◽  
House Dust Mites ◽  
Dust Mites ◽  
Der P 1 ◽  
The Many ◽  
Euroglyphus Maynei

AbstractHouse dust mites live in house dusts and affect the health of humans. Among the many species, Dermatophagoides farinae, D. pteronyssinus, and Euroglyphus maynei have been found to be commonly associated with Ig-E-mediated allergic diseases. As a result, there is increasing effort to develop methods for the diagnosis and treatment of diseases caused by these species. The purpose of the current study was to explore the evolutionary relationships among house dust mites. After adult D. farinae were separated and isolated for total RNA extract, the cDNA coding for Der f 1 and Der f 2 were cloned and sequenced. Then amino acid sequences for group 1 and 2 allergens of two of the most common house dust mites, D. pteronyssinus, E. maynei, were obtained from databases. Interestingly, homological analysis of amino acid sequences showed that both Der p 1 and Der p 2 from D. pteronyssinus had more similarities to Eur m 1 and Eur m 2, respectively, than they had to Der f 1 and Der f 2 from D. farinae. In the phylogenetic trees, D. pteronyssinus clustered with E. maynei, but not with D. farinae, although D. pteronyssinus and D. farinae belong to the same genus according to morphological taxonomy. It was previously assumed that D. pteronyssinus was more similar to E. maynei than to D. farinae at evolutionary levels.

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