scholarly journals Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

2013 ◽  
Vol 96 (11) ◽  
pp. 6955-6964 ◽  
Author(s):  
B.G. Botaro ◽  
C.S. Cortinhas ◽  
L.V. Março ◽  
J.F.G. Moreno ◽  
L.F.P. Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

Detection and Enumeration of Streptococcus agalactiae from Bovine Milk Samples by Real-Time Polymerase Chain Reaction

2015 ◽  
Vol 71 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Nara Ladeira de Carvalho ◽  
Juliano Leonel Gonçalves ◽  
Bruno Garcia Botaro ◽  
Luis Felipe de Prada e Silva ◽  
Marcos Veiga dos Santos
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

scholarly journals PCR Assay With Host Specific Internal Control for Staphylococcus Aureus From Bovine Milk Samples

2015 ◽  
Vol 38 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Zafer Cantekin ◽  
Yasar Ergun ◽  
Hasan Solmaz ◽  
Gamze Özge Özmen ◽  
Melek Demir ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
False Negative ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Specific Primers ◽  
Milk Samples ◽  
Negative Results ◽  
False Negative Results ◽  
Polymerase Chain

Abstract Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.

scholarly journals Daignosis of Staphylococcus aureus mastitis in bovine in Al-Najaf province by using Polymerase chain reaction (PCR)

2013 ◽  
Vol 12 (2) ◽  
pp. 81
Author(s):  
N. A. Al- Anbagi
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Subclinical Mastitis ◽  
Molecular Method ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Significant Incidence ◽  
Left Posterior ◽  
Polymerase Chain

This study was conducted to collect 388 milk samples from cows at different villages and townships in Al-Najaf province to examine about Staphylococcus aureus mastitis .CMT was used for subclinical mastitis screening ,212(54.6%) milk samples were mastitic .The molecular method (PCR assay) was used to detected the presence (glpF) gene in classically diagnosed S.aureus, which appeared that 38(92.6%) S.aureus mastitis as 13(32.5%) clinical and 25(14.5%) subclinical mastitis .There was high significant incidence of Staphylococcus aureus mastitis in left posterior udder quarter rather than others quarters

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