scholarly journals DETECTION OF COXIELLA BURNETII IN BOVINE MILK SAMPLES USING POLYMERASE CHAIN REACTION

2009 ◽  
Vol 55 (123) ◽  
pp. 1-9
Keyword(s):  
Polymerase Chain Reaction ◽  
Coxiella Burnetii ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

scholarly journals Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

2013 ◽  
Vol 96 (11) ◽  
pp. 6955-6964 ◽  
Author(s):  
B.G. Botaro ◽  
C.S. Cortinhas ◽  
L.V. Março ◽  
J.F.G. Moreno ◽  
L.F.P. Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

Detection and Enumeration of Streptococcus agalactiae from Bovine Milk Samples by Real-Time Polymerase Chain Reaction

2015 ◽  
Vol 71 (3) ◽  
pp. 363-372 ◽  
Author(s):  
Nara Ladeira de Carvalho ◽  
Juliano Leonel Gonçalves ◽  
Bruno Garcia Botaro ◽  
Luis Felipe de Prada e Silva ◽  
Marcos Veiga dos Santos
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Streptococcus Agalactiae ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

scholarly journals PCR Assay With Host Specific Internal Control for Staphylococcus Aureus From Bovine Milk Samples

2015 ◽  
Vol 38 (1) ◽  
pp. 97-100 ◽  
Author(s):  
Zafer Cantekin ◽  
Yasar Ergun ◽  
Hasan Solmaz ◽  
Gamze Özge Özmen ◽  
Melek Demir ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
False Negative ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Specific Primers ◽  
Milk Samples ◽  
Negative Results ◽  
False Negative Results ◽  
Polymerase Chain

Abstract Staphylococcus aureus is considered as one of the most important and common pathogens of bovine mastitis. Polymerase Chain Reaction is frequently proposed in the diagnosis of S. aureus directly from milk samples instead of classical culture. However, false-negative results may occur in the polymerase chain reaction analysis performed directly from clinical material. For the purpose of disclosing the false negative results, the use of internal amplification controls can be beneficial. Therefore, in this study a new polymerase chain reaction technique with host specific internal amplification control was developed by optimizing S. aureus-specific primers in combination with bovine specific primers. The effectiveness of the developed technique in this study was attempted in milk samples from bovine subclinical mastitis. This technique has the potential to detect S. aureus from bovine milk samples or dairy products.

scholarly journals Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province

2013 ◽  
Vol 12 (2) ◽  
pp. 101
Author(s):  
Gh. K. A. Al-kuzaay ◽  
Q. H. Kshash
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Agalactiae ◽  
Specific Primer ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Bacteriological Testing ◽  
Polymerase Chain ◽  
Pcr Test

This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).

scholarly journals Evaluating the bacterial culture technique by Polymerase chain reaction for the diagnosis of Brucella abortus in milk of cows suspected with brucellosis.

2016 ◽  
Vol 40 (1) ◽  
pp. 5-8
Author(s):  
Bashar Sadeq Noomy
Keyword(s):  
Polymerase Chain Reaction ◽  
Brucella Abortus ◽  
Bacterial Culture ◽  
Culture Technique ◽  
Test Results ◽  
Chain Reaction ◽  
Culture Techniques ◽  
Milk Samples ◽  
Polymerase Chain ◽  
Pcr Test

      The aim of this study is to determine the sensitivity of bacterial culture technique in the detection of Brucella abortus in milk samples of aborted cows. Sixty samples of milk were collected from aborted cows during a period which did not exceed two months after the abortion. All of them were positive for rose bengal test. Results showed that Brucella abortus was isolated from 7 out of 60 (11.6%) from the milk of aborted cows, while PCR test showed that 32 out of 60 (53.3%) milk sample contained Brucella abortus. The specificity of culture techniques was 10%, but its sensitivity was only 21.8%. Beside the cautions in dealing with live Brucella abortus (as culture), it is also less sensitive than PCR, though it is better to use PCR technique in the diagnosis of brucellosis in aborted cows milk.

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