scholarly journals Daignosis of Staphylococcus aureus mastitis in bovine in Al-Najaf province by using Polymerase chain reaction (PCR)

2013 ◽  
Vol 12 (2) ◽  
pp. 81
Author(s):  
N. A. Al- Anbagi
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Subclinical Mastitis ◽  
Molecular Method ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Significant Incidence ◽  
Left Posterior ◽  
Polymerase Chain

This study was conducted to collect 388 milk samples from cows at different villages and townships in Al-Najaf province to examine about Staphylococcus aureus mastitis .CMT was used for subclinical mastitis screening ,212(54.6%) milk samples were mastitic .The molecular method (PCR assay) was used to detected the presence (glpF) gene in classically diagnosed S.aureus, which appeared that 38(92.6%) S.aureus mastitis as 13(32.5%) clinical and 25(14.5%) subclinical mastitis .There was high significant incidence of Staphylococcus aureus mastitis in left posterior udder quarter rather than others quarters

scholarly journals Streptococcus agalactiae mastitis of bovine detection by Polymerase Chain Reaction (PCR) test in AL-Diwanyia province

2013 ◽  
Vol 12 (2) ◽  
pp. 101
Author(s):  
Gh. K. A. Al-kuzaay ◽  
Q. H. Kshash
Keyword(s):  
Polymerase Chain Reaction ◽  
Streptococcus Agalactiae ◽  
Specific Primer ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Bacteriological Testing ◽  
Polymerase Chain ◽  
Pcr Test

This study was conducted for exam 348 milk samples from (clinically mastitic and other healthy cows) in many areas in AL-Diwanyia province by using CMT and bacteriological testing , which appeared that (64.9%) as percentage of mastitis ( clinically 15.9% , subclinically 84.0% ) Streptococcus agalactiae mastitis 13.2% ( 26.6% clinically , 73.3 % subclinicaly) diagnose by PCR assay by using specific primer (16SrRNA). Streptococcus agalactiae (30 isolates) after classical methods applied for streptococcus agalactiae identification (86 isolates).

scholarly journals Detection and enumeration of Staphylococcus aureus from bovine milk samples by real-time polymerase chain reaction

2013 ◽  
Vol 96 (11) ◽  
pp. 6955-6964 ◽  
Author(s):  
B.G. Botaro ◽  
C.S. Cortinhas ◽  
L.V. Março ◽  
J.F.G. Moreno ◽  
L.F.P. Silva ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Bovine Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain

scholarly journals Identification of subclinical mastitis caused by Mycoplasma spp. from screenings of bulk tanks

2018 ◽  
Vol 70 (6) ◽  
pp. 1793-1797
Author(s):  
S.F. Joaquim ◽  
F.F. Guimarães ◽  
A. Salina ◽  
N.B. Junqueira ◽  
E.N. Gomes ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Subclinical Mastitis ◽  
Mammary Glands ◽  
Dairy Herds ◽  
Mycoplasma Bovis ◽  
Chain Reaction ◽  
Milk Samples ◽  
Polymerase Chain ◽  
Bulk Tank ◽  
Mycoplasma Spp

ABSTRACT Mastitis caused by Mycoplasma spp., regardless of species, are considered highly contagious pathogens and, usually was not responsive to antimicrobial therapy. Five dairy herds, comprising 489 animals and 1,956 mammary glands, were used in this study. Milk samples were obtained from bulk tanks and subjected to polymerase chain reaction (PCR) for the identification of Mollicutes, Mycoplasma spp., and Mycoplasma bovis. Moreover, individual samples from cases of clinical and subclinical mastitis in quarters of the dairy herds’ animals that yielded a positive PCR upon bulk tank analysis were subjected to molecular analysis. Only one bulk tank was positive for class Mollicutes by PCR. All positive samples classified as mastitis teats had their DNA extracted and tested by PCR for both class Mollicutes and M. bovis. Of these, two (2.08%) were positive for Mycoplasma genus, although none was positive for M. bovis. This result suggests that the PCR of bulk tanks is a viable tool in monitoring and preventing mastitis infections caused by Mycoplasma spp.

scholarly journals Positive nasal Staphylococcus aureus polymerase chain reaction assay is not sensitive in predicting concurrent or subsequent Staphylococcus aureus infection in critically ill patients

2020 ◽  
Vol 48 (3) ◽  
pp. 196-202
Author(s):  
Kalai C Kanagasingham ◽  
Kwok M Ho ◽  
J Owen Robinson
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Critically Ill ◽  
Sensitivity And Specificity ◽  
Critically Ill Patients ◽  
High Specificity ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Polymerase Chain ◽  
Mssa Infection

Staphylococcal infection is associated with significant morbidity and mortality in critically ill patients. Using data from 16,681 patients who had a nasal Staphylococcus aureus polymerase chain reaction (PCR) assay on admission to the intensive care unit (ICU) of Royal Perth Hospital between March 2006 and September 2016, this retrospective cohort study assessed whether nasal S. aureus colonisation on admission to an ICU was predictive of concurrent or subsequent S. aureus infections. Culture-proven S. aureus infections were identified using the hospital microbiology database. Of the 16,681 patients included, 565 (3.4%) had a positive methicillin-resistant S. aureus (MRSA) assay, 146 (0.9%) had a positive methicillin-sensitive S. aureus (MSSA) assay and eight (0.05%) had both positive MRSA and MSSA assays. Of those 565 patients with a positive MRSA PCR assay, 79 (13.8%) had concurrent or subsequent MRSA infections. Of those 146 patients with a positive MSSA PCR assay, only 5 (3.4%) had MSSA infection. The sensitivity and specificity for the MRSA PCR assay in predicting concurrent or subsequent MRSA infection were 72.7% (95% confidence intervals (CI) 63.4%–80.8%) and 97.0% (95% CI 96.8%–97.3%), respectively. The sensitivity and specificity for the MSSA PCR assay in predicting concurrent or subsequent MSSA infection were 3.3% (95% CI 1.1%–7.6%) and 99.1% (95% CI 98.9%–99.2%), respectively. Both nasal MRSA and MSSA PCR assays had a high specificity and negative predictive value in predicting MRSA and MSSA infections, respectively, suggesting that in centres without endemic S. aureus infections, a negative nasal MRSA or MSSA PCR assay may be useful to reduce unnecessary empirical antibiotic therapy against S. aureus.

Multiplex polymerase chain reaction as a mastitis screening test for Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis in bulk milk samples

2003 ◽  
Vol 70 (2) ◽  
pp. 149-155 ◽  
Author(s):  
Patchara Phuektes ◽  
Glenn F Browning ◽  
Garry Anderson ◽  
Peter D Mansell
Keyword(s):  
Polymerase Chain Reaction ◽  
Multiplex Polymerase Chain Reaction ◽  
Streptococcus Dysgalactiae ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Milk Samples ◽  
Streptococcus Uberis ◽  
Bulk Milk ◽  
Polymerase Chain ◽  
Total Bacteria

Effective diagnostic tools for screening herds for mastitis pathogens are important in development and monitoring of mastitis control programmes. A multiplex polymerase chain reaction (PCR) assay for simultaneous detection of Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis was used in preliminary studies to assess its applicability as an alternative method for monitoring mastitis caused by these organisms at the herd level. PCR was used to detect the presence of these organisms in bulk milk samples. Correlations with bulk milk somatic cell counts (BMCC), total bacteria counts and thermoduric bacteria counts were evaluated. A total of 176 bulk milk samples were collected from 42 herds on five consecutive occasions at approx. 10-d intervals. Str. uberis was the most common organism in these bulk milk samples. There was no relationship between presence of either Staph. aureus, Str. dysgalactiae or Str. uberis and BMCC, total bacteria counts or thermoduric bacteria counts. However, presence of Str. agalactiae was associated with high BMCC and total bacteria counts. The results of this study show that regular analysis of bulk milk using this multiplex PCR assay may be a useful tool for monitoring herd status with respect to Str. agalactiae, but is of less value for monitoring occurrence of Staph. aureus, Str. dysgalactiae and Str. uberis. Further investigations are needed to clarify the relationship between positive PCR results and the prevalence of infected cows in the herd.

scholarly journals Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction

2019 ◽  
Vol 12 (1) ◽  
pp. 68-71 ◽  
Author(s):  
Mustofa Helmi Effendi ◽  
Mirza Atikah Madarina Hisyam ◽  
Poedji Hastutiek ◽  
Wiwiek Tyasningsih
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Dairy Farms ◽  
Raw Milk ◽  
Chain Reaction ◽  
Milk Samples ◽  
Epidemiological Tool ◽  
Gene Polymerase Chain Reaction ◽  
Polymerase Chain ◽  
Coa Gene

Aim: This study was conducted to study the coagulase (coa) gene-based genetic diversity of Staphylococcus aureus, isolated from different samples of cattle from three different regions in East Java Province, Indonesia. Materials and Methods: A total of 160 raw milk samples collected in East Java Province, Indonesia, were screened for the presence of S. aureus. The presumptive isolates were confirmed by coa test. The confirmed S. aureus isolates were subjected to coa gene polymerase chain reaction. Results: Of 160 different samples, 20 (12.5%) isolates of S. aureus were confirmed by positive coa test. Of 20 S. aureus isolates, 19 (95%) isolates carried coa gene. Six different genotypes of coa gene, i.e., 440 bp, 510 bp, 547 bp, 680 bp, 740 bp, and 820 bp were obtained. One coa genotypes, 510 bp (10 isolates) were observed in polymorphism to be more prevalent than the others, and the genotype was present in at least one isolates from every region. Conclusion: It can be concluded that coa gene is easily epidemiological tool for detection of variation strain from S. aureus.

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