Effects on enteric methane production and bacterial and archaeal communities by the addition of cashew nut shell extract or glycerol—An in vitro evaluation

ABSTRACT This study evaluated the in vitro digestibility of nutrients from different diets added with chitosan (Q), technic cashew nut shell liquid (LCC) and the association of Q and LCC. The treatments used consisted of 4 diets (forage: concentrate ratio of 100: 0, 50:50, 40:60 and 20:80) associated with 4 additives (control, chitosan, LCC and the association of Q + LCC), totaling 16 treatments, in a 4x4 factorial randomized block design. The dosages used were: Control (without additives), LCC (600mg/kg DM), Chitosan (900mg/kg DM), and LCCQ (600mg/kg LCC DM + 900mg/kg Chitosan DM). In the laboratory, samples were analyzed for IVDMD, IVNDFD, IVCPD, pH and RAN (ruminal ammonia nitrogen). For pH and RAN analyses, samples were taken at 0, 2, 4, 6 and 8 hours after incubation. The results showed higher digestibility of DM, NDF and CP for diets with chitosan and technic cashew nut shell liquid alone and higher pH and RAN values in the diets containing the two additives. The association of additives brings better results for animal nutrition and increases ruminant productivity.

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In vitro evaluation of cashew nut shell liquid as a methane-inhibiting and propionate-enhancing agent for ruminants

Journal of Dairy Science ◽

10.3168/jds.2009-2754 ◽

2010 ◽

Vol 93 (11) ◽

pp. 5258-5267 ◽

Cited By ~ 42

Author(s):

Y. Watanabe ◽

R. Suzuki ◽

S. Koike ◽

K. Nagashima ◽

M. Mochizuki ◽

...

Keyword(s):

Cashew Nut Shell Liquid ◽

In Vitro Evaluation ◽

Cashew Nut ◽

Cashew Nut Shell

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In vitro evaluation, in vivo quantification, and microbial diversity studies of nutritional strategies for reducing enteric methane production

Tropical Animal Health and Production ◽

10.1007/s11250-011-9992-0 ◽

2011 ◽

Vol 44 (5) ◽

pp. 953-964 ◽

Cited By ~ 19

Author(s):

Adibe Luiz Abdalla ◽

Helder Louvandini ◽

Sobhy Mohamed Abdallah Hassan Sallam ◽

Ives Cláudio da Silva Bueno ◽

Siu Mui Tsai ◽

...

Keyword(s):

Microbial Diversity ◽

Methane Production ◽

In Vitro Evaluation ◽

Enteric Methane ◽

Diversity Studies ◽

Nutritional Strategies

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Reducing enteric methane production from buffalo (Bubalus bubalis) by garlic oil supplementation in in vitro rumen fermentation system

SN Applied Sciences ◽

10.1007/s42452-021-04264-6 ◽

2021 ◽

Vol 3 (2) ◽

Author(s):

Avijit Dey ◽

Shyam Sundar Paul ◽

Puran Chand Lailer ◽

Satbir Singh Dahiya

Keyword(s):

Environmental Pollution ◽

Methane Production ◽

Rumen Fluid ◽

Bubalus Bubalis ◽

Rumen Fermentation ◽

Acetyl Esterase ◽

Garlic Oil ◽

Enteric Methane ◽

In Vitro Rumen Fermentation

AbstractEnteric methane production contributes significantly to the greenhouse gas emission globally. Although, buffaloes are integral part of livestock production in Asian countries, contributing milk, meat and draft power, the contribution of enteric methane to environmental pollution attracts attention. The present study investigated the efficacy of garlic (Allium sativum) oil in reducing enteric methane production from buffaloes (Bubalus bubalis) by in vitro rumen fermentation. Garlic oil (GOL) was tested at four concentrations [0 (Control), 33.33 µl (GOL-1), 83.33 µl (GOL-2) and 166.66 µl (GOL-3) per litre of buffered rumen fluid] in 100-ml graduated glass syringes and incubated at 39℃ for 24 h for in vitro rumen fermentation study. Supplementation of GOL-1 increased (p < 0.05) total gas production in comparison with GOL-3; however, it remained comparable (p > 0.05) with control and GOL-2. Graded doses of garlic oil inclusions reduced (p < 0.001) methane concentration (%) in total gas and total methane production (ml/g DM), irrespective of concentrations. The feed degradability, volatile fatty acids and microbial biomass production (MBP) were not affected (p > 0.05) by GOL-1, but these tended to decrease in GOL-2 with marked reduction (p < 0.01) in GOL-3. The decrease (p < 0.01) in NH3–N concentration in fermentation fluid in the presence of garlic oil, irrespective of concentration, suggests reduced deamination by inhibiting rumen proteolytic bacterial population. The activities of ruminal fibrolytic enzymes (CMCase, xylanase, β-glucosidase, acetyl esterase) were not affected by lower dose (GOL-1) of garlic oil; however, reduction (p < 0.05) of these enzymes activity in rumen liquor was evident at higher doses (GOL-2 and GOL-3) of supplementation. This study shows positive impact of garlic oil supplementation at low dose (33.33 µl/l of rumen fluid) in reducing enteric methane production, thereby, abatement of environmental pollution without affecting feed digestibility.

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In vitro gas production kinetics and digestibility in ruminant diets with different levels of cashew nut shell liquid

Semina Ciências Agrárias ◽

10.5433/1679-0359.2018v39n4p1669 ◽

2018 ◽

Vol 39 (4) ◽

pp. 1669

Author(s):

Tatiana García Díaz ◽

Antonio Ferriani Branco ◽

Luís Carlos Vinhas Ítavo ◽

Geraldo Tadeu dos Santos ◽

Silvana Teixeira Carvalho ◽

...

Keyword(s):

Gas Production ◽

Ammoniacal Nitrogen ◽

Ruminal Fermentation ◽

Cashew Nut Shell Liquid ◽

Ruminal Ph ◽

Cashew Nut ◽

Production Kinetics ◽

Ruminant Diets ◽

Cashew Nut Shell

The aim of this study was to evaluate the effect of increasing levels of cashew nut shell liquid (CNSL) in ruminant diets on in vitro dry matter digestibility (IVDMD), gas production kinetics, ruminal fermentation parameters, ammoniacal nitrogen concentration (NH3-N), and pH of the artificial rumen contents. The experimental design was completely randomized in a factorial 5 x 4 + 1 design, with five concentrate levels (200, 400, 600, 800, and 1,000 g kg-1 DM) and four CNSL levels (0, 0.3, 0.6, and 1.2 g kg-1 DM), as well as a control diet comprising only whole-plant corn silage, totaling 21 treatments. The inclusion of concentrate linearly increased IVDMD, while CNSL levels showed a quadratic effect, with the maximum estimated at 0.5 g kg-1 of CNSL. The total gas production, the disappearance of the substrate, the fraction of slow degradation (fraction VF2), and the respective degradation rate (fraction µ2) linearly increased with increasing levels of concentrate in the diet. Increasing concentrate levels resulted in a linear increase in the concentration of NH3-N and a reduction in the pH of the rumen liquid. Increasing CNSL levels decreased the concentration of NH3-N and increased the ruminal pH. The inclusion of 0.5 g CNSL kg-1 in the ruminant diets improved IVDMD, without altering the kinetic parameters of ruminal fermentation. The addition of CNSL to ruminant diets reduces ammoniacal nitrogen production and can avoid drastic reductions in ruminal pH, favoring better fermentation in the rumen.

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In vitro methane production as influenced by harvest date and level of nitrogen application

Proceedings of the British Society of Animal Science ◽

10.1017/s1752756200013223 ◽

2003 ◽

Vol 2003 ◽

pp. 163-163

Author(s):

D. K. Lovett ◽

A. Bortolozzo ◽

P. O’Kiely ◽

P. Conaghan ◽

F. P. O’Mara

Keyword(s):

Methane Production ◽

Production Systems ◽

Livestock Production ◽

Harvest Date ◽

Nitrogen Application ◽

N Level ◽

Grazed Pasture ◽

Enteric Methane ◽

Fertiliser Application

Increasing animal productivity increases daily enteric methane (CH4) emissions but reduces CH4 output per unit of animal production. Irish livestock production systems are characterised by a high dependence on grazed pasture. Increased nitrogen (N) fertiliser application can increase animal productivity through increased intake. The objective of this trial was to assess the effect of N level application and pasture maturity on in vitro methane production.

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