scholarly journals Measurement of Ruminal Volatile Fatty Acid Production from Alfalfa Hay or Corn Silage Rations Using a Continuous Infusion Isotope Dilution Technique

1968 ◽  
Vol 51 (11) ◽  
pp. 1823-1830 ◽  
Author(s):  
W.J. Esdale ◽  
G.A. Broderick ◽  
L.D. Satter
Keyword(s):  
Fatty Acid ◽  
Continuous Infusion ◽  
Isotope Dilution ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Corn Silage ◽  
Dilution Technique ◽  
Fatty Acid Production ◽  
Isotope Dilution Technique ◽  
Alfalfa Hay

An investigation into the loss of activity of 14C-labelled volatile fatty acid (VFA) tracers used to measure VFA production rates in the rumen of sheep

1977 ◽  
Vol 88 (1) ◽  
pp. 245-246 ◽  
Author(s):  
S. Wilson ◽  
J. C. Macrae
Keyword(s):  
Fatty Acid ◽  
Isotope Dilution ◽  
Volatile Fatty Acid ◽  
Room Temperature ◽  
Standard Technique ◽  
Personal Communication ◽  
Deionized Water ◽  
Dilution Technique ◽  
Isotope Dilution Technique ◽  
Grassland Research

Few problems have been reported with the use of the isotope dilution technique for measuring volatile fatty acid (VFA) production in the rumen of sheep. However, early studies carried out in this laboratory, using a standard technique (Leng & Leonard, 1965) showed serious limitations associated with losses of activity from the infusates. Subsequent inquiries suggested that other laboratories had experienced similar problems (J. D. Sutton, National Institute for Research in Dairying, Shinfield, and D. E. Beever, Grassland Research Institute, Hurley, personal communication). When solutions of [U-14C]Na acetate, [l·14C]Na propionate and [l·14C]Na butyrate obtained from the Radiochemical Centre, Amersham, were each diluted with deionized water to provide 0·5 μtCi μtmol/ml (hereafter designated 14Ac, 14Pr and 14Bu respectively) and left to stand at room temperature for 6 h, losses of activity of C, 15 and 3% respectively were found.

DRY MATTER LOSS AND VOLATILE FATTY ACID PRODUCTION IN THE ARTIFICIAL RUMEN AS INDICES OF FORAGE QUALITY

1958 ◽  
Vol 38 (2) ◽  
pp. 171-180 ◽  
Author(s):  
J. M. Asplund ◽  
R. T. Berg ◽  
L. W. McElroy ◽  
W. J. Pigden
Keyword(s):  
Fatty Acid ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Dry Matter ◽  
Nutritive Value ◽  
Rumen Fluid ◽  
Fatty Acid Production ◽  
Dry Matter Loss ◽  
Dry Matter Digestibility ◽  
Alfalfa Hay

Procedures are described for determining dry matter losses and for measuring volatile fatty acid production by direct chromatographic separation of acids produced during incubation of finely ground forages in miniature artificial rumen units. The results of preliminary assays done on three samples of unknown digestibility—excellent alfalfa hay, good mixed grass-alfalfa hay, and oat straw—showed that significantly different percentages of dry matter were lost and significantly different amounts of fatty acids were produced when feeds of such diverse quality were fermented. Analysis of the data for two groups of 11 and 6 samples of hay, for which dry matter digestibility coefficients as determined with sheep were available, indicated the existence of a high degree of correlation between dry matter digestibility in vivo and both dry matter loss and fatty acid production in vitro. Differences in assay results were observed when filtered rumen fluid or washed cells from the rumen fluid of sheep fed hay or from sheep fed straw were employed as sources of inocula. Examples of unsolved problems which are pertinent to the appraisal of the efficacy of artificial rumen assays for the estimation of the nutritive value of forages are discussed.

A Techno-Economic case for Volatile Fatty Acid Production for Increased Sustainability in the Wastewater Treatment Industry

2021 ◽  
Author(s):  
Ganesh K Veluswamy ◽  
Andy Ball ◽  
Richard Dinsdale ◽  
Alan Guwy ◽  
Kalpit Shah
Keyword(s):  
Wastewater Treatment ◽  
Fatty Acid ◽  
Anaerobic Digestion ◽  
Acid Production ◽  
Volatile Fatty Acid ◽  
Fugitive Emissions ◽  
Fatty Acid Production

Methane, the final product of methanogenesis during anaerobic digestion is a low value product (0.1$/m3). Concerns over fugitive emissions from methane coupled with recent reduction in costs of solar and...

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