Genetic analysis for resistance to leaf curl disease in Chilli Peppers (Capsicum annuum L.) under specific situations

Leaf curl is a serious viral disease of chilli caused by a group of bigomoviruses dominated by chilli leaf curl virus (ChiLcv). With the aim to study the mode of inheritance of the ChiLcv disease resistance, a resistant genotype DLS-Sel.10 was crossed with a susceptible genotype Phule Mukta and F1, F2, B1 and B2 generations were developed. The parents along with the segregating generations were screened under natural conditions as well as challenged inoculation with viruliferous whiteflies carrying predominant ChiLcv.PCR amplification of viral genome-specific marker confirmed the presence of virus in all the tested plants however, only susceptible plants produced symptoms. The F1 plants showed susceptibility under both natural and challenged inoculation conditions indicating that the resistance is of recessive nature. On Chi-square test, it was found that susceptible and resistant plants of the two F2populations segregated in a ratio of 3 susceptible : 1 resistant plants and the B2 population derived from DLS-Sel.10/Phule Mukta//DLS-Sel.10 segregated in a ratio of 1 resistant : 1 susceptible plant suggesting that the Chilcv is goverened by a single recessive gene. The findings of this study will help in breeding for ChiLcv resistance in Chilli.

Download Full-text

SUSCEPTIBILITY OF CHILLI LINES / VARIETIES TOWARDS CHILLI LEAF CURL VIRUS AND ITS MANAGEMENT THROUGH VECTOR CONTROL

Pakistan Journal of Phytopathology ◽

10.33866/phytopathol.029.01.0304 ◽

2017 ◽

Vol 29 (1) ◽

pp. 17 ◽

Cited By ~ 1

Author(s):

Muhammad S. Hussain ◽

Khalid Naveed ◽

Muhammad Atiq

Keyword(s):

Disease Incidence ◽

Field Trials ◽

Local Germplasm ◽

Moderately Resistant ◽

Leaf Curl Virus ◽

Chilli Leaf Curl Virus ◽

Leaf Curl Disease ◽

Whitefly Vector ◽

Susceptible Response ◽

Leaf Curl

Chilli leaf curl virus (ChiLCV) is a serious problem for chilli production in Pakistan and India. In this study, fourteen different chilli lines / varieties were screened for their resistance/susceptibility towards ChiLCV in open field trials. Data of disease severity and disease incidence was recorded on weekly basis. Out of all varieties screened, Tatapuri Chilli and CH111 showed high susceptibility towards the chilli leaf curl disease. Talhari, CH 99, CH103, CH 106, CH107, CH108, CH109, GSL111 showed susceptible response whereas CBS1292 showed moderately susceptible response towards the disease. Only two cultivars Hybrid-46 and Hot Queen were found as moderately resistant. The screening experiment showed that most of the chilli varieties are susceptible to chilli leaf curl disease and resistance is lacking in local germplasm. Furthermore, three insecticides: Imidacloprid, Polo and Emamectin were tested for their efficacy for the management of disease and whitefly vector in field conditions at weekly intervals. Out of three insecticides, Polo gave best results as compared to Emamectin and Imidacloprid against chilli leaf curl disease and whitefly vector.

Download Full-text

Molecular characterization of Chilli leaf curl virus and satellite molecules associated with leaf curl disease of Amaranthus spp

Virus Genes ◽

10.1007/s11262-013-1027-7 ◽

2013 ◽

Vol 48 (2) ◽

pp. 397-401 ◽

Cited By ~ 14

Author(s):

B. George ◽

R. Vinoth Kumar ◽

S. Chakraborty

Keyword(s):

Molecular Characterization ◽

Amaranthus Spp ◽

Leaf Curl Virus ◽

Chilli Leaf Curl Virus ◽

Leaf Curl Disease ◽

Leaf Curl

Download Full-text

Association of a monopartite chilli leaf curl virus and defective betasatellite molecule isolated from leaf curl disease affected papaya in India

The Journal of Horticultural Science and Biotechnology ◽

10.1080/14620316.2021.1874837 ◽

2021 ◽

pp. 1-6

Author(s):

Abhinav Kumar ◽

Nishtha Kashyap ◽

Rohit Gupta

Keyword(s):

Leaf Curl Virus ◽

Chilli Leaf Curl Virus ◽

Leaf Curl Disease ◽

Betasatellite Molecule ◽

Leaf Curl

Download Full-text

Virus-vector Relationships, Host Range, Detection and Sequence Comparison of Chilli leaf curl virus Associated with an Epidemic of Leaf Curl Disease of Chilli in Jodhpur, India

Journal of Phytopathology ◽

10.1111/j.1439-0434.2011.01876.x ◽

2012 ◽

Vol 160 (3) ◽

pp. 146-155 ◽

Cited By ~ 26

Author(s):

D. M. J. B. Senanayake ◽

Anupam Varma ◽

Bikash Mandal

Keyword(s):

Host Range ◽

Sequence Comparison ◽

Virus Vector ◽

Leaf Curl Virus ◽

Chilli Leaf Curl Virus ◽

Leaf Curl Disease ◽

Leaf Curl

Download Full-text

Association of recombinant Chilli leaf curl virus with enation leaf curl disease of tomato: a new host for chilli begomovirus in India

Phytoparasitica ◽

10.1007/s12600-016-0510-9 ◽

2016 ◽

Vol 44 (2) ◽

pp. 213-223 ◽

Cited By ~ 7

Author(s):

V. Venkataravanappa ◽

P. Swarnalatha ◽

C. N. Lakshminarayana Reddy ◽

Neha Chauhan ◽

M. Krishna Reddy

Keyword(s):

New Host ◽

Leaf Curl Virus ◽

Chilli Leaf Curl Virus ◽

Leaf Curl Disease ◽

Leaf Curl

Download Full-text

Characterization of novel French bean leaf curl virus and betasatellite associated with leaf curl disease of French bean (Phaseolus vulgaris L.)

Journal of General Plant Pathology ◽

10.1007/s10327-020-00979-3 ◽

2021 ◽

Author(s):

V. Venkataravanappa ◽

Priti Sonavane ◽

C. N. Lakshminarayana Reddy ◽

M. Krishna Reddy

Keyword(s):

Phaseolus Vulgaris ◽

French Bean ◽

Phaseolus Vulgaris L ◽

Leaf Curl Virus ◽

Leaf Curl Disease ◽

Leaf Curl

Download Full-text

Resistance Analysis of CRISPR/Cas9 Genome-Edited Chili M2 Mutant Lines against Pepper Yellow Leaf Curl Viral Disease

Jurnal AgroBiogen ◽

10.21082/jbio.v17n1.2020.p1-10 ◽

2021 ◽

Vol 17 (1) ◽

pp. 1

Author(s):

Wandy Murti Prasetya ◽

Toto Hadiarto ◽

Wening Enggarini ◽

Aqwin Polosoro ◽

Suharsono Suharsono

Keyword(s):

Viral Disease ◽

Chili Pepper ◽

Nuclear Antigen ◽

Pcr Analysis ◽

Yellow Leaf ◽

Resistant Lines ◽

Mutant Lines ◽

Leaf Curl Virus ◽

Proliferating Cell ◽

Leaf Curl

<p>Pepper yellow leaf curl virus (PepYLCV) infection transmitted by silverleaf whitefly (Bemisia tabaci [Gennadius]) can decrease chili pepper yield up to 100%. At this moment, there is no chili pepper variety resistant to PepYLCV available. Genome editing approach through CRISPR/Cas9 is an effort to develop variety resistance to the viral infection. The purpose of this study was to obtain M2 lines developed by CRISPR/Cas9 system on proliferating cell nuclear antigen (PCNA) gene for resistance to PepYLCV. A total of four M2 lines (C47-7, L84-2, L84-23, and L120-19) consisting of 60 chili plants were tested for their resistance to PepYLCV. PCR analysis was performed to detect the presence (infection) of the virus. The results showed that a total of 35 plants derived from the four lines were resistant to PepYLCV. They consisted of 7 plants from C47-7 line, 11 plants from L84-2 line, 9 plants from L84-23 line, and 8 plants from L120-19 line. PCR analysis confirmed that the resistant plants obtained from this study were negatively infected by the virus. Since not all tested plants were resistant to virus infection, the PCNA gene allele in these resistant lines were most likely heterozigotes. Sequencing of PCNA gene of the resistant lines is needed to confirm that the resistance phenotypes obtained was due to mutation of the gene. Therefore, further selection needs to be performed to obtain stable and PepYLCV-resistant lines.</p>

Download Full-text

POLIO VACCINATION

The Professional Medical Journal ◽

10.29309/tpmj/2018.25.01.540 ◽

2018 ◽

Vol 25 (01) ◽

pp. 67-72

Author(s):

Ahmad Ali ◽

Liaqat Ali ◽

Mussawar Shah ◽

Naimatullah Khan ◽

Muhammad Shafee ◽

...

Keyword(s):

Viral Disease ◽

Cultural Barriers ◽

Religious Institutions ◽

Test Statistics ◽

Chi Square ◽

Religious Background ◽

Polio Vaccine ◽

Polio Vaccination ◽

Chi Square Test ◽

Infantile Paralysis

Background: Polio is a viral disease causing infantile paralysis in the community.This study cultural and traditional barriers to Polio Vaccination. Pakistan in order to explore thecultural and traditional barriers in vaccination against polio. Setting: Tehsil Kwazakhela, Swat,KPK. Methods: A total of 200 respondents were selected from 364 household’s population. Aconceptual framework based on cultural barriers to polio vaccination (dependent variable) andimpediments to polio vaccination (dependent variable) was worked out and uni-variate analysisfor percentage and Chi square test statistics was carried out for association between variables.Results: At uni-variate level majority of the respondent’s i.e. 98% argued that misconceptionis a great issue in the area and 98.5% respondents treat the new born through traditional way.Furthermore, 79.5% masses were in favor of clergy’s fatwah and 95% were agreed that poliovaccine caused immodesty, 81.5% were facing hurdles due to religious background, and 68 %responded that polio vaccination affected from terrorism, 92% agreed that polio vaccination isagainst the prevalent culture. At bivariate level myth, clergies fatwah, polio vaccine is an Americanploy and common superstition spread by radicals were found significant with impediments topolio vaccination. Conclusion: A well patronized campaign over media through proper displayof victims from polio with explicit display of agonies from the victims is essential. Support fromclergies and other regional religious institutions will help in the success of campaign to fightagainst Polio.

Download Full-text