Genetics of white rust resistance in Indian mustard (Brassica juncea L.) and its validation on using molecular markers

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
V. V. Singh ◽  
Monika Dubey ◽  
Neeraj Gurjar ◽  
Balbeer . ◽  
Priyamedha . ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Banding Pattern ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Indian Mustard ◽  
Single Dominant Gene ◽  
White Rust ◽  
Resistant Genotypes

White rust resistance loci (AcB1-A4.1 and AcB1-A5.1) associated with intron polymorphic (IP) markers i.e. At5g41560 and At2g36360, respectively, were used for validation of P1, P2, F1, F2, BC1F1 and BC2F1 generations. The donor parents namely, Bio-YSR and BEC-144 produced desired banding pattern of 430 and 750 bp while recipients viz., NRCHB 101 and DRMR-150-35 exhibited different pattern from donors confirming white rust resistance loci 4.1 and 5.1 with marker At5g41560 and At2g36360, respectively. Confirmation of these set of two IP markers in the parents and F1s lead us to further screening of selected F2, BC1F1 and BC2F1 populations. Available data on white rust reaction in different generations under study revealed that single dominant gene is responsible for white rust resistance. Potential of molecular markers in developing white rust resistant genotypes is proved under present study.

Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.)

2015 ◽  
Vol 95 (5) ◽  
pp. 939-945 ◽  
Author(s):  
Binay K. Singh ◽  
Divakar Nandan ◽  
Supriya Ambawat ◽  
Bhagirath Ram ◽  
Arun Kumar ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Rust Resistance ◽  
Indian Mustard ◽  
Resistance Locus ◽  
White Rust ◽  
Pcr Products ◽  
Percent Disease Index ◽  
Different Populations

Singh, B. K., Nandan, D., Supriya, A., Ram, B., Kumar, A., Singh, T., Meena, H. S., Kumar, V., Singh, V. V., Rai, P. K. and Singh, D. 2015. Validation of molecular markers for marker-assisted pyramiding of white rust resistance loci in Indian Mustard (Brassica juncea L.). Can. J. Plant Sci. 95: 939–945. Successful application of molecular markers in marker-assisted pyramiding relies on effective determination of the target phenotype. In this respect, evaluation of the efficiency of markers for marker-assisted selection through cross-validation in different genetic backgrounds and in different populations is a crucial step. In the present study, the previously identified Arabidopsis-derived intron polymorphic (IP) markers At5g41560 and At2g36360, which were highly linked with AcB1-A4.1 and AcB1-A5.1, respectively, were validated in a set of 25 genotypes of Indian Mustard and in three different F2 populations. The relationships between the variation of PCR products of the two markers with the percent disease index (PDI) of the tested genotypes, and the co-segregation analysis of the markers with disease phenotype in F2 populations clearly indicated that At5g41560 and At2g36360 are genotype-nonspecific markers and are closely linked to white rust resistance loci AcB1-A4.1 and AcB1-A5.1, respectively. It also became evident from the present study that AcB1-A4.1 and an another white rust resistance locus Ac(2)t are likely the same gene locus.

Molecular markers linked to white rust resistance in mustard Brassica juncea

1998 ◽  
Vol 97 (5-6) ◽  
pp. 865-870 ◽  
Author(s):  
K. V. Prabhu ◽  
D. J. Somers ◽  
G. Rakow ◽  
R. K. Gugel
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Rust Resistance ◽  
White Rust

INHERITANCE OF CROWN RUST RESISTANCE IN THREE ACCESSIONS OF AVENA STERILIS

1980 ◽  
Vol 22 (1) ◽  
pp. 27-33 ◽  
Author(s):  
D. E. Harder ◽  
R. I. H. McKenzie ◽  
J. W. Martens
Keyword(s):  
Rust Resistance ◽  
Dominant Gene ◽  
Crown Rust ◽  
Inheritance Of Resistance ◽  
Single Dominant Gene ◽  
Avena Sterilis ◽  
Oat Crown Rust ◽  
Crown Rust Resistance ◽  
Resistance Spectrum ◽  
Dominant Genes

The inheritance of resistance to oat crown rust was studied in three accessions of Avena sterilis L. Accession CAV 4274 originated from Morocco, CAV 4540 from Algeria, and CAV 3695 from Tunisia. Seedling rust tests on F2 backcross families indicated the presence of two dominant genes for crown rust resistance in CAV 4274. One of these, a gene conditioning resistance to most races tested, was linked or allelic to gene Pc-38, and was designated gene Pc-62. The second gene conferred resistance only to one of the six races studied, and was not tested further. In CAV 4540, a single dominant gene, Pc-63 was possibly allelic with Pc-62 and linked or allelic to Pc-38. Genes Pc-62 and 63 are generally similar to Pc-38 in their resistance spectrum, but these three genes are differentiated by races CR 102, CR 103, and CR 107. A single dominant gene in CAV 3695 appeared to be Pc-50.

Hybridization between resistant and susceptible fenugreek accessions and evaluation of Cercospora leaf spot resistance in segregating generations

2020 ◽  
Vol 100 (1) ◽  
pp. 1-15
Author(s):  
U. Subedi ◽  
S. Acharya ◽  
S. Chatterton ◽  
J. Thomas ◽  
D. Friebel
Keyword(s):  
Leaf Spot ◽  
Dominant Gene ◽  
Single Dominant Gene ◽  
Narrow Sense ◽  
Cercospora Leaf Spot ◽  
Narrow Sense Heritability ◽  
Trigonella Foenum Graecum ◽  
Resistant Genes ◽  
Simple Pathway ◽  
Resistant Genotypes

Cercospora leaf spot (CLS), caused by Cercospora traversoana, is an important phyto-pathological problem of self-pollinated fenugreek (Trigonella-foenum graecum). Developing resistant genotypes in crop plants has been considered the best option to control diseases for economic, environmental, and social reasons. However, before this can be accomplished, knowledge about the inheritance of disease-resistant genes is necessary for creating high-yielding resistant genotypes. One susceptible fenugreek cultivar, Tristar, and two resistant accessions L3717 and PI138687 were used in two-way crosses using hand emasculation and pollination technique in a greenhouse. F1 plants were grown in a greenhouse and allowed to grow till maturity to produce F2 seeds. Some flowers from F1 plants were crossed back to both resistant and susceptible parents separately to generate backcross (BC1) seeds. Parents, F1, F2, and BC1 populations were grown in the greenhouse using a RCBD with four replications. Plants were inoculated 30 d after sowing with a suspension of C. traversoana at 2 × 105 conidia mL−1. Symptoms were observed and rated on individual plants 25 d after inoculation, and plants were categorized according to susceptible or resistant reactions based on rating scores. Mean disease score was significantly different (p < 0.0001) among generations. In both the cross combinations, results showed CLS resistance in fenugreek (from L3717 and PI138687) was governed by a single dominant gene which is moderately heritable (46% narrow sense heritability). This indicates a relatively simple pathway for transfer of genes to adapted fenugreek cultivars.

scholarly journals The Arabidopsis TIR-NB-LRR Gene RAC1 Confers Resistance to Albugo candida (White Rust) and Is Dependent on EDS1 but not PAD4

2004 ◽  
Vol 17 (7) ◽  
pp. 711-719 ◽  
Author(s):  
Mohammad H. Borhan ◽  
Eric B. Holub ◽  
Jim L. Beynon ◽  
Kevin Rozwadowski ◽  
S. Roger Rimmer
Keyword(s):  
Positional Cloning ◽  
Rust Resistance ◽  
Interleukin 1 ◽  
Dominant Gene ◽  
Leucine Rich Repeat ◽  
Albugo Candida ◽  
White Rust ◽  
Candida Isolate ◽  
Turn Structure ◽  
Lrr Domain

Resistance to Albugo candida isolate Acem1 is conferred by a dominant gene, RAC1, in accession Ksk-1 of Arabidopsis thaliana. This gene was isolated by positional cloning and is a member of the Drosophila toll and mammalian interleukin-1 receptor (TIR) nucleotide-binding site leucine-rich repeat (NB-LRR) class of plant resistance genes. Strong identity of the TIR and NB domains was observed between the predicted proteins encoded by the Ksk-1 allele and the allele from an Acem1-susceptible accession Columbia (Col) (99 and 98%, respectively). However, major differences between the two predicted proteins occur within the LRR domain and mainly are confined to the β-strand/β-turn structure of the LRR. Both proteins contain 14 imperfect repeats. RAC1-mediated resistance was analyzed further using mutations in defense regulation, including: pad4-1, eds1-1, and NahG, in the presence of the RAC1 allele from Ksk-1. White rust resistance was completely abolished by eds1-1 but was not affected by either pad4-1 or NahG.

scholarly journals Genetics of stem rust resistance in four popular durum wheat cultivars

2020 ◽  
Vol 80 (02) ◽  
Author(s):  
A. Divya ◽  
T. L. Prakasha ◽  
S. Chand ◽  
A. N. Mishra ◽  
V. G. Dubey ◽  
...  
Keyword(s):  
Durum Wheat ◽  
Resistance Genes ◽  
Stem Rust ◽  
Rust Resistance ◽  
Dominant Gene ◽  
Central India ◽  
Stem Rust Resistance ◽  
Single Dominant Gene ◽  
Wheat Cultivars ◽  
Segregation Data

A study was conducted to understand the mode of inheritance and extent of diversity of stem rust resistance in four popular durum wheat cultivars of central India viz., HI 8498 (Malav Shakti), HI 8663 (Poshan), HI 8713 (Pusa Mangal) and HI 8737 (Pusa Anmol) using Puccinia graminis tritici (Pgt) pathotypes 15-1 (123G15) and 40-3 (127G29). These cultivars were crossed with susceptible parents i.e., Motia and Malvi Local and were also crossed among themselves in half diallel fashion. The F2 and F3 segregation data revealed that a single dominant gene each controlled resistance to the pathotype 40-3 in HI 8713 and HI 8663, while two dominant genes each governed resistance to this pathotype in HI 8737 and HI 8498. A single dominant gene each conditioned resistance to the pathotype 15-1 in all the four cultivars. The F2 segregation data of the intercrosses among the resistant parents showed that three different resistance genes controlled resistance among four cultivars against each Pgt pathotype 40-3 and 15-1. These genes seem to be different from the most commonly postulated stem rust resistance genes in Indian durum wheat germplasm viz., Sr11, Sr12, Sr7b and Sr9e which are ineffective/less effective against the test pathotypes. Hence, the genes identified in the present study can be utilized in broadening the genetic base of stem rust resistance in Indian durum wheat.

Single independent genes confer resistance to faba bean rust (Uromyces viciae-fabae) in the current Australian cultivar Doza and a central European line Ac1655

2016 ◽  
Vol 67 (6) ◽  
pp. 649 ◽  
Author(s):  
Kedar N. Adhikari ◽  
Peng Zhang ◽  
Abdus Sadeque ◽  
Sami Hoxha ◽  
Richard Trethowan
Keyword(s):  
Faba Bean ◽  
Rust Resistance ◽  
Animal Feed ◽  
Dominant Gene ◽  
Segregation Ratio ◽  
Human Consumption ◽  
Single Dominant Gene ◽  
Bean Rust ◽  
Resistant Parent ◽  
Independent Gene

Faba bean (Vicia faba L.) is one of the oldest grain legumes and is grown in many countries for both human consumption and animal feed. Faba bean rust, caused by Uromyces viciae-fabae, is a serious disease of faba beans in the subtropical agricultural region of Australia. Experiments were conducted to assess the genetic variation for rust resistance in Australian faba bean germplasm and to determine the genetic basis of rust resistance in selected germplasm. Resistant lines were identified, subsequently crossed to agronomically suitable parents and the ensuing progeny were evaluated for resistance. Many derived lines showed a higher level of resistance than the current cultivars, although none were rated immune. This level of resistance was considered adequate for reliable crop production when combined with limited fungicide application. Genetic studies from the seedling test of F2 and F3 progenies derived from two crosses based on two different sources of resistance showed three distinct responses; highly resistant, moderately resistant and susceptible. However, no homozygous family with a moderate response was found in the F3 progeny test, hence, this infection type could not be attributed to independent gene(s). The segregation ratio in both F2 and F3 in the population derived from Doza#12035, a selection from the commercial cultivar Doza, indicated a single dominant gene was responsible for conferring resistance. In the other population developed from the resistant parent Ac1655, it is likely that also a single dominant gene confers resistance even though the F3 segregation ratio deviated significantly from a one gene hypothesis. An allelism test revealed that each of the resistant parents (Doza#12035 and Ac1655) carried a single and independent gene for resistance, thus providing at least two genes for breeders to choose or pyramid for improving the rust resistance in faba bean.

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