scholarly journals SPREADSHEET AND ITERATION DEAD TIMES OF 2- ALKANONES HOMOLOGUES SERIES ELUTED BY ACETONITRILE/WATER IN REVERSED PHASE HIGHPERFORMANCE LIQUID CHROMATOGRAPHY

2021 ◽  
Vol 14 (02) ◽  
pp. 1041-1048
Author(s):  
R. Idroes ◽  
M. Mahmudi ◽  
S. Saiful ◽  
M. Muslem ◽  
G.M. Idroes ◽  
...  
Author(s):  
Kazuki Akira ◽  
Yui Matsumoto ◽  
Takao Hashimoto

AbstractCarbonyl stress compounds such as glyoxal and methylglyoxal have been recently attracting much attention because of their possible clinical significance in chronic and age-related diseases. A high-performance liquid chromatographic procedure has been developed for the simultaneous quantitation of glyoxal and methylglyoxal in human urine. The assay is based on the reaction of these compounds with 1,2-diamino-4,5-dimethoxybenzene to form fluorescent adducts, which are separated by reversed-phase highperformance liquid chromatography in a total run time of 45 minutes and quantitated fluorometrically using 2,3-pentanedione as an internal standard. Derivatization is performed for diluted urine (100–120 mOsm/kg H


Author(s):  
Raju Chandra ◽  
Manisha Pant ◽  
Harchan Singh ◽  
Deepak Kumar ◽  
Ashwani Sanghi

A reliable and reproducible reversed-phase high performance liquid chromatography (RP-HPLC) was developed for the quantitative determination of Remipril drug content from marketed bulk tablets. The active ingredient of Remipril separation achieved with C18 column using the methanol water mobile phase in the ratio of 40:60 (v/v). The active ingredient of the drug content quantify with UV detector at 215 nm. The retention time of Remipril is 5.63 min. A good linearity relation (R2=0.999) was obtained between drug concentration and average peak areas. The limit of detection and limit of quantification of the instrument were calculated 0.03 and 0.09 µg/mL, respectively. The accuracy of the method validation was determined 102.72% by recoveries method.


Author(s):  
Ling DING ◽  
Jun DONG ◽  
Yuan-Sheng XIAO ◽  
Xiu-Li ZHANG ◽  
Xing-Ya XUE ◽  
...  

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