Qualitative and quantitative analysis of polyphenolic compounds from clone plants of Actaea racemosa L. by high-performance thin layer chromatography and reversed-phase high-performance liquid chromatography

Planta Medica ◽  
2015 ◽  
Vol 81 (16) ◽  
Author(s):  
M Bittner ◽  
R Schenk ◽  
MF Melzig
2010 ◽  
Vol 8 (3) ◽  
pp. 530-535 ◽  
Author(s):  
Dumitru Condrat ◽  
Cristina Mosoarca ◽  
Alina Zamfir ◽  
Florin Crişan ◽  
Maria Szabo ◽  
...  

AbstractThis study presents the results obtained from qualitative and quantitative analysis of gallic acid from hydro-alcoholic extracts (methanol, ethanol) of plants from Plantae regnum. Plant qualitative analysis was performed using a novel mass spectrometric (MS) method based on fully automated chip-nanoelectrospray ionization (nanoESI) high capacity ion trap (HCT) while quantitative analysis was carried out by high performance liquid chromatography (HPLC). These methods were applied to Alchemilla vulgaris — common lady’s-mantle (aerial part), Allium ursinum — bear’s garlic (leaves), Acorus calamus — common sweet flag (roots), Solidago virga-aurea — goldenrod (aerial part). Obtained results indicated that methanol extracts (96%, 80%) have a gallic acid content ranging between 0.0011–0.0576 mg mL−1 extract while the ethanol extracts (96%, 60%) exhibit a gallic acid concentration that varies between 0.0010–0.0182 mg mL−1 extract.


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